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Q5SJ76 (RS15_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
30S ribosomal protein S15
Gene names
Name:rpsO
Ordered Locus Names:TTHA1138
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length89 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA By similarity. HAMAP-Rule MF_01343

Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome. HAMAP-Rule MF_01343

Subunit structure

Part of the 30S ribosomal subunit. Forms a bridge to the 50S subunit in the 70S ribosome, contacting the 23S rRNA. Ref.4 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17

Sequence similarities

Belongs to the ribosomal protein S15P family.

Mass spectrometry

Molecular mass is 10424 Da from positions 2 - 89. Determined by MALDI. Ref.5

Ontologies

Keywords
   LigandRNA-binding
rRNA-binding
   Molecular functionRibonucleoprotein
Ribosomal protein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentribosome

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionrRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

structural constituent of ribosome

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 898830S ribosomal protein S15 HAMAP-Rule MF_01343
PRO_0000115574

Secondary structure

............. 89
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q5SJ76 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 9DB7E1C533DBF340

FASTA8910,554
        10         20         30         40         50         60 
MPITKEEKQK VIQEFARFPG DTGSTEVQVA LLTLRINRLS EHLKVHKKDH HSHRGLLMMV 

        70         80 
GQRRRLLRYL QREDPERYRA LIEKLGIRG 

« Hide

References

« Hide 'large scale' references
[1]"Molecular genetic and protein chemical characterization of the cytochrome ba3 from Thermus thermophilus HB8."
Keightley J.A., Zimmermann B.H., Mather M.W., Springer P., Pastuszyn A., Lawrence D.M., Fee J.A.
J. Biol. Chem. 270:20345-20358(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
[3]"Purification and characterization of the 30S ribosomal proteins from the bacterium Thermus thermophilus."
Tsiboli P., Herfurth E., Choli T.
Eur. J. Biochem. 226:169-177(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-22.
[4]"Identification of an RNA-protein bridge spanning the ribosomal subunit interface."
Culver G.M., Cate J.H.D., Yusupova G.Z., Yusupov M.M., Noller H.F.
Science 285:2133-2135(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERSUBUNIT BRIDGE FORMATION.
[5]"Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry."
Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A.
Proteomics 5:4818-4831(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY.
[6]"Structure of a bacterial 30S ribosomal subunit at 5.5 A resolution."
Clemons W.M. Jr., May J.L.C., Wimberly B.T., McCutcheon J.P., Capel M.S., Ramakrishnan V.
Nature 400:833-840(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE 30S SUBUNIT.
[7]"Structure of the 30S ribosomal subunit."
Wimberly B.T., Brodersen D.E., Clemons W.M. Jr., Morgan-Warren R.J., Carter A.P., Vonrhein C., Hartsch T., Ramakrishnan V.
Nature 407:327-339(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.05 ANGSTROMS) OF THE 30S SUBUNIT.
[8]"Structure of functionally activated small ribosomal subunit at 3.3 A resolution."
Schluenzen F., Tocilj A., Zarivach R., Harms J., Gluehmann M., Janell D., Bashan A., Bartels H., Agmon I., Franceschi F., Yonath A.
Cell 102:615-623(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) OF THE 30S SUBUNIT.
[9]"The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit."
Brodersen D.E., Clemons W.M. Jr., Carter A.P., Morgan-Warren R.J., Wimberly B.T., Ramakrishnan V.
Cell 103:1143-1154(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) OF THE 30S SUBUNIT.
[10]"Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics."
Carter A.P., Clemons W.M. Jr., Brodersen D.E., Morgan-Warren R.J., Wimberly B.T., Ramakrishnan V.
Nature 407:340-348(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 30S SUBUNIT.
[11]"Structure of the S15,S6,S18-rRNA complex: assembly of the 30S ribosome central domain."
Agalarov S.C., Prasad G.S., Funke P.M., Stout C.D., Williamson J.R.
Science 288:107-113(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF A 30S SUBUNIT FRAGMENT.
[12]"The path of messenger RNA through the ribosome."
Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F.
Cell 106:233-241(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
[13]"Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3."
Pioletti M., Schluenzen F., Harms J., Zarivach R., Gluehmann M., Avila H., Bashan A., Bartels H., Auerbach T., Jacobi C., Hartsch T., Yonath A., Franceschi F.
EMBO J. 20:1829-1839(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF THE 30S SUBUNIT.
[14]"Crystal structure of an initiation factor bound to the 30S ribosomal subunit."
Carter A.P., Clemons W.M. Jr., Brodersen D.E., Morgan-Warren R.J., Hartsch T., Wimberly B.T., Ramakrishnan V.
Science 291:498-501(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF THE 30S SUBUNIT.
[15]"Crystal structure of the ribosome at 5.5 A resolution."
Yusupov M.M., Yusupova G.Z., Baucom A., Lieberman K., Earnest T.N., Cate J.H.D., Noller H.F.
Science 292:883-896(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME, INTERSUBUNIT BRIDGE FORMATION.
[16]"Recognition of cognate transfer RNA by the 30S ribosomal subunit."
Ogle J.M., Brodersen D.E., Clemons W.M. Jr., Tarry M.J., Carter A.P., Ramakrishnan V.
Science 292:897-902(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.11 ANGSTROMS) OF THE 30S SUBUNIT.
[17]"Crystal structure of the 30S ribosomal subunit from Thermus thermophilus: structure of the proteins and their interactions with 16S RNA."
Brodersen D.E., Clemons W.M. Jr., Carter A.P., Wimberly B.T., Ramakrishnan V.
J. Mol. Biol. 316:725-768(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.05 ANGSTROMS) OF THE 30S SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L09121 Genomic DNA. Translation: AAD38865.1.
AP008226 Genomic DNA. Translation: BAD70961.1.
PIRT52484.
RefSeqYP_144404.1. NC_006461.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1FJGX-ray3.00O1-89[»]
1FKAX-ray3.30O1-89[»]
1G1XX-ray2.60B/G2-89[»]
1GIXX-ray5.50R1-89[»]
1HNWX-ray3.40O1-89[»]
1HNXX-ray3.40O1-89[»]
1HNZX-ray3.30O1-89[»]
1HR0X-ray3.20O1-89[»]
1I94X-ray3.20O2-89[»]
1I95X-ray4.50O2-89[»]
1I96X-ray4.20O2-89[»]
1I97X-ray4.50O2-89[»]
1IBKX-ray3.31O1-89[»]
1IBLX-ray3.11O1-89[»]
1IBMX-ray3.31O1-89[»]
1J5EX-ray3.05O2-89[»]
1JGOX-ray5.60R1-89[»]
1JGPX-ray7.00R1-89[»]
1JGQX-ray5.00R1-89[»]
1L1Umodel-O1-89[»]
1ML5electron microscopy14.00R1-89[»]
1N32X-ray3.00O2-89[»]
1N33X-ray3.35O2-89[»]
1N34X-ray3.80O2-89[»]
1N36X-ray3.65O2-89[»]
1PNXX-ray9.50O2-89[»]
1QD7X-ray5.50H21-70[»]
1XMOX-ray3.25O1-89[»]
1XMQX-ray3.00O1-89[»]
1XNQX-ray3.05O1-89[»]
1XNRX-ray3.10O1-89[»]
1YL4X-ray5.50R1-89[»]
2B64X-ray5.90O1-89[»]
2B9MX-ray6.76O1-89[»]
2B9OX-ray6.46O1-89[»]
2E5LX-ray3.30O2-89[»]
2F4VX-ray3.80O1-89[»]
2HGIX-ray5.00R1-89[»]
2HGPX-ray5.50R1-89[»]
2HGRX-ray4.51R1-89[»]
2HHHX-ray3.35O1-89[»]
2J00X-ray2.80O2-88[»]
2J02X-ray2.80O2-89[»]
2OW8X-ray3.71p-[»]
2UU9X-ray3.10O2-89[»]
2UUAX-ray2.90O2-89[»]
2UUBX-ray2.80O2-89[»]
2UUCX-ray3.10O2-89[»]
2UXBX-ray3.10O2-89[»]
2UXCX-ray2.90O2-89[»]
2UXDX-ray3.20O2-89[»]
2V46X-ray3.50O2-89[»]
2V48X-ray3.50O2-89[»]
2VQEX-ray2.50O1-89[»]
2VQFX-ray2.90O1-89[»]
2WDGX-ray3.30O1-89[»]
2WDHX-ray3.30O1-89[»]
2WDKX-ray3.50O1-89[»]
2WDMX-ray3.50O1-89[»]
2WH1X-ray3.45O1-89[»]
2WH3X-ray3.45O1-89[»]
2WRIX-ray3.60O1-89[»]
2WRKX-ray3.60O1-89[»]
2WRNX-ray3.60O1-89[»]
2WRQX-ray3.60O1-89[»]
2X9RX-ray3.10O1-89[»]
2X9TX-ray3.10O1-89[»]
2XFZX-ray3.20O1-89[»]
2XG1X-ray3.20O1-89[»]
2XQDX-ray3.10O1-89[»]
2XSYelectron microscopy7.80O1-89[»]
2XUYelectron microscopy7.60O1-89[»]
2Y0UX-ray3.10O1-89[»]
2Y0WX-ray3.10O1-89[»]
2Y0YX-ray3.10O1-89[»]
2Y10X-ray3.10O1-89[»]
2Y12X-ray3.10O1-89[»]
2Y14X-ray3.10O1-89[»]
2Y16X-ray3.10O1-89[»]
2Y18X-ray3.10O1-89[»]
2ZKQelectron microscopy8.70o1-89[»]
2ZM6X-ray3.30O2-89[»]
3FICelectron microscopy6.40O2-89[»]
3HUWX-ray3.10O1-89[»]
3HUYX-ray3.10O1-89[»]
3I8GX-ray3.10R1-89[»]
3I8HX-ray3.10R1-89[»]
3I9BX-ray3.50R1-89[»]
3I9DX-ray3.50R1-89[»]
3KIQX-ray3.30o1-89[»]
3KISX-ray3.30o1-89[»]
3KIUX-ray3.60o1-89[»]
3KIXX-ray3.60o1-89[»]
3KNHX-ray3.00O1-89[»]
3KNJX-ray3.15O1-89[»]
3KNLX-ray3.45O1-89[»]
3KNNX-ray3.45O1-89[»]
3OGEX-ray3.00O1-89[»]
3OGYX-ray3.00O1-89[»]
3OHCX-ray3.00O1-89[»]
3OHDX-ray3.00O1-89[»]
3OHYX-ray3.00O1-89[»]
3OI0X-ray3.00O1-89[»]
3OI2X-ray3.10O1-89[»]
3OI4X-ray3.10O1-89[»]
3OTOX-ray3.69O1-89[»]
3T1HX-ray3.11O1-89[»]
3T1YX-ray2.80O1-89[»]
3TVFX-ray3.10R1-89[»]
3TVGX-ray3.10R1-89[»]
3UXSX-ray3.20O1-89[»]
3UXTX-ray3.20O1-89[»]
3UYDX-ray3.00R1-89[»]
3UYFX-ray3.00R1-89[»]
3UZ3X-ray3.30R1-89[»]
3UZ4X-ray3.30R1-89[»]
3UZ6X-ray3.00R1-89[»]
3UZ7X-ray3.00R1-89[»]
3UZGX-ray3.30R1-89[»]
3UZIX-ray3.30R1-89[»]
3UZLX-ray3.30R1-89[»]
3UZMX-ray3.30R1-89[»]
3V22X-ray3.00O1-89[»]
3V24X-ray3.00O1-89[»]
3V26X-ray3.10O1-89[»]
3V28X-ray3.10O1-89[»]
3V2CX-ray2.70O1-89[»]
3V2EX-ray2.70O1-89[»]
3V6UX-ray3.90O1-89[»]
3V6VX-ray3.90O1-89[»]
3ZN7X-ray3.10O1-89[»]
3ZNDX-ray3.10O1-89[»]
3ZVOX-ray3.80O1-89[»]
4ABRX-ray3.10O1-89[»]
4AQYX-ray3.50O2-89[»]
4B3MX-ray2.90O2-89[»]
4B3RX-ray3.00O2-89[»]
4B3SX-ray3.15O2-89[»]
4B3TX-ray3.00O2-89[»]
4B8FX-ray3.70O1-89[»]
4B8HX-ray3.70O1-89[»]
4BYDX-ray3.35O1-89[»]
4CR1X-ray2.95O1-89[»]
4DH9X-ray3.20O1-89[»]
4DHBX-ray3.20O1-89[»]
4DR1X-ray3.60O1-89[»]
4DR2X-ray3.25O1-89[»]
4DR3X-ray3.35O1-89[»]
4DR4X-ray3.97O1-89[»]
4DR5X-ray3.45O1-89[»]
4DR6X-ray3.30O1-89[»]
4DR7X-ray3.75O1-89[»]
4DUYX-ray3.39O1-89[»]
4DUZX-ray3.65O1-89[»]
4DV0X-ray3.85O1-89[»]
4DV1X-ray3.85O1-89[»]
4DV2X-ray3.65O1-89[»]
4DV3X-ray3.55O1-89[»]
4DV4X-ray3.65O1-89[»]
4DV5X-ray3.68O1-89[»]
4DV6X-ray3.30O1-89[»]
4DV7X-ray3.29O1-89[»]
4EJ9X-ray3.52O1-89[»]
4EJAX-ray3.52O1-89[»]
4G5KX-ray3.30R1-89[»]
4G5MX-ray3.30R1-89[»]
4G5TX-ray3.10R1-89[»]
4G5VX-ray3.10R1-89[»]
4GKJX-ray3.30O2-89[»]
4GKKX-ray3.20O2-89[»]
4JI0X-ray3.49O1-89[»]
4JI1X-ray3.14O1-89[»]
4JI2X-ray3.64O1-89[»]
4JI3X-ray3.35O1-89[»]
4JI4X-ray3.69O1-89[»]
4JI5X-ray3.85O1-89[»]
4JI6X-ray3.55O1-89[»]
4JI7X-ray3.50O1-89[»]
4JI8X-ray3.74O1-89[»]
4JUWX-ray2.86O2-89[»]
4JV5X-ray3.16O2-89[»]
4JYAX-ray3.10O2-89[»]
4K0KX-ray3.40O2-89[»]
4K0LX-ray3.30O2-89[»]
4K0PX-ray3.30O2-89[»]
4KHPX-ray3.10O2-89[»]
4NVUX-ray3.00O1-89[»]
4NVWX-ray3.00O1-89[»]
4NVYX-ray3.10O1-89[»]
4NW0X-ray3.10O1-89[»]
4NXMX-ray3.65O1-89[»]
4NXNX-ray3.54O1-89[»]
4OX9X-ray3.80O2-89[»]
ProteinModelPortalQ5SJ76.
SMRQ5SJ76. Positions 2-89.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING300852.TTHA1138.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD70961; BAD70961; BAD70961.
GeneID3169463.
KEGGttj:TTHA1138.
PATRIC23957235. VBITheThe93045_1117.

Phylogenomic databases

eggNOGCOG0184.
HOGENOMHOG000040097.
KOK02956.
OMANKQDHHS.
OrthoDBEOG6B368J.
PhylomeDBQ5SJ76.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-1170-MONOMER.

Family and domain databases

Gene3D1.10.287.10. 1 hit.
HAMAPMF_01343_B. Ribosomal_S15_B.
InterProIPR000589. Ribosomal_S15.
IPR005290. Ribosomal_S15_bac-type.
IPR009068. S15_NS1_RNA-bd.
[Graphical view]
PfamPF00312. Ribosomal_S15. 1 hit.
[Graphical view]
SUPFAMSSF47060. SSF47060. 1 hit.
TIGRFAMsTIGR00952. S15_bact. 1 hit.
PROSITEPS00362. RIBOSOMAL_S15. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ5SJ76.

Entry information

Entry nameRS15_THET8
AccessionPrimary (citable) accession number: Q5SJ76
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references