Q5SJ76 (RS15_THET8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
30S ribosomal protein S15

Gene names

Name:	rpsO
Ordered Locus Names:	TTHA1138

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus

Protein attributes

Sequence length	89 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA By similarity. HAMAP-Rule MF_01343 Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome. HAMAP-Rule MF_01343
Subunit structure	Part of the 30S ribosomal subunit. Forms a bridge to the 50S subunit in the 70S ribosome, contacting the 23S rRNA. Ref.4 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17
Sequence similarities	Belongs to the ribosomal protein S15P family.
Mass spectrometry	Molecular mass is 10424 Da from positions 2 - 89. Determined by MALDI. Ref.5

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Cellular_component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.3

Chain

2 – 89

88

30S ribosomal protein S15 HAMAP-Rule MF_01343

PRO_0000115574

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

89

Helix Strand Turn

Details...

Helix

5 – 13

9

Beta strand

17 – 20

4

Beta strand

22 – 24

3

Helix

25 – 42

18

Turn

43 – 45

3

Helix

50 – 73

24

Helix

75 – 85

11

Sequences

	Sequence		Length	Mass (Da)
	Q5SJ76 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 9DB7E1C533DBF340	FASTA	89	10,554
	10 20 30 40 50 60 MPITKEEKQK VIQEFARFPG DTGSTEVQVA LLTLRINRLS EHLKVHKKDH HSHRGLLMMV 70 80 GQRRRLLRYL QREDPERYRA LIEKLGIRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular genetic and protein chemical characterization of the cytochrome ba3 from Thermus thermophilus HB8." Keightley J.A., Zimmermann B.H., Mather M.W., Springer P., Pastuszyn A., Lawrence D.M., Fee J.A. J. Biol. Chem. 270:20345-20358(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.
[3]	"Purification and characterization of the 30S ribosomal proteins from the bacterium Thermus thermophilus." Tsiboli P., Herfurth E., Choli T. Eur. J. Biochem. 226:169-177(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-22.
[4]	"Identification of an RNA-protein bridge spanning the ribosomal subunit interface." Culver G.M., Cate J.H.D., Yusupova G.Z., Yusupov M.M., Noller H.F. Science 285:2133-2135(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERSUBUNIT BRIDGE FORMATION.
[5]	"Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry." Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A. Proteomics 5:4818-4831(2005) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY.
[6]	"Structure of a bacterial 30S ribosomal subunit at 5.5 A resolution." Clemons W.M. Jr., May J.L.C., Wimberly B.T., McCutcheon J.P., Capel M.S., Ramakrishnan V. Nature 400:833-840(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE 30S SUBUNIT.
[7]	"Structure of the 30S ribosomal subunit." Wimberly B.T., Brodersen D.E., Clemons W.M. Jr., Morgan-Warren R.J., Carter A.P., Vonrhein C., Hartsch T., Ramakrishnan V. Nature 407:327-339(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.05 ANGSTROMS) OF THE 30S SUBUNIT.
[8]	"Structure of functionally activated small ribosomal subunit at 3.3 A resolution." Schluenzen F., Tocilj A., Zarivach R., Harms J., Gluehmann M., Janell D., Bashan A., Bartels H., Agmon I., Franceschi F., Yonath A. Cell 102:615-623(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) OF THE 30S SUBUNIT.
[9]	"The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit." Brodersen D.E., Clemons W.M. Jr., Carter A.P., Morgan-Warren R.J., Wimberly B.T., Ramakrishnan V. Cell 103:1143-1154(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) OF THE 30S SUBUNIT.
[10]	"Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics." Carter A.P., Clemons W.M. Jr., Brodersen D.E., Morgan-Warren R.J., Wimberly B.T., Ramakrishnan V. Nature 407:340-348(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 30S SUBUNIT.
[11]	"Structure of the S15,S6,S18-rRNA complex: assembly of the 30S ribosome central domain." Agalarov S.C., Prasad G.S., Funke P.M., Stout C.D., Williamson J.R. Science 288:107-113(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF A 30S SUBUNIT FRAGMENT.
[12]	"The path of messenger RNA through the ribosome." Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F. Cell 106:233-241(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
[13]	"Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3." Pioletti M., Schluenzen F., Harms J., Zarivach R., Gluehmann M., Avila H., Bashan A., Bartels H., Auerbach T., Jacobi C., Hartsch T., Yonath A., Franceschi F. EMBO J. 20:1829-1839(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF THE 30S SUBUNIT.
[14]	"Crystal structure of an initiation factor bound to the 30S ribosomal subunit." Carter A.P., Clemons W.M. Jr., Brodersen D.E., Morgan-Warren R.J., Hartsch T., Wimberly B.T., Ramakrishnan V. Science 291:498-501(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF THE 30S SUBUNIT.
[15]	"Crystal structure of the ribosome at 5.5 A resolution." Yusupov M.M., Yusupova G.Z., Baucom A., Lieberman K., Earnest T.N., Cate J.H.D., Noller H.F. Science 292:883-896(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME, INTERSUBUNIT BRIDGE FORMATION.
[16]	"Recognition of cognate transfer RNA by the 30S ribosomal subunit." Ogle J.M., Brodersen D.E., Clemons W.M. Jr., Tarry M.J., Carter A.P., Ramakrishnan V. Science 292:897-902(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.11 ANGSTROMS) OF THE 30S SUBUNIT.
[17]	"Crystal structure of the 30S ribosomal subunit from Thermus thermophilus: structure of the proteins and their interactions with 16S RNA." Brodersen D.E., Clemons W.M. Jr., Carter A.P., Wimberly B.T., Ramakrishnan V. J. Mol. Biol. 316:725-768(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.05 ANGSTROMS) OF THE 30S SUBUNIT.
+	Additional computationally mapped references.

Web resources

T.thermophilus ribosome structure and function

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L09121 Genomic DNA. Translation: AAD38865.1.
AP008226 Genomic DNA. Translation: BAD70961.1.

PIR

T52484.

RefSeq

YP_144404.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FJG	X-ray	3.00	O	1-89	[»]
1FKA	X-ray	3.30	O	1-89	[»]
1G1X	X-ray	2.60	B/G	2-89	[»]
1GIX	X-ray	5.50	R	1-89	[»]
1HNW	X-ray	3.40	O	1-89	[»]
1HNX	X-ray	3.40	O	1-89	[»]
1HNZ	X-ray	3.30	O	1-89	[»]
1HR0	X-ray	3.20	O	1-89	[»]
1I94	X-ray	3.20	O	2-89	[»]
1I95	X-ray	4.50	O	2-89	[»]
1I96	X-ray	4.20	O	2-89	[»]
1I97	X-ray	4.50	O	2-89	[»]
1IBK	X-ray	3.31	O	1-89	[»]
1IBL	X-ray	3.11	O	1-89	[»]
1IBM	X-ray	3.31	O	1-89	[»]
1J5E	X-ray	3.05	O	2-89	[»]
1JGO	X-ray	5.60	R	1-89	[»]
1JGP	X-ray	7.00	R	1-89	[»]
1JGQ	X-ray	5.00	R	1-89	[»]
1L1U	model	-	O	1-89	[»]
1N32	X-ray	3.00	O	2-89	[»]
1N33	X-ray	3.35	O	2-89	[»]
1N34	X-ray	3.80	O	2-89	[»]
1N36	X-ray	3.65	O	2-89	[»]
1QD7	X-ray	5.50	H	21-70	[»]
1XMO	X-ray	3.25	O	1-89	[»]
1XMQ	X-ray	3.00	O	1-89	[»]
1XNQ	X-ray	3.05	O	1-89	[»]
1XNR	X-ray	3.10	O	1-89	[»]
1YL4	X-ray	5.50	R	1-89	[»]
2B64	X-ray	5.90	O	1-89	[»]
2B9M	X-ray	6.76	O	1-89	[»]
2B9O	X-ray	6.46	O	1-89	[»]
2E5L	X-ray	3.30	O	2-89	[»]
2F4V	X-ray	3.80	O	1-89	[»]
2HGI	X-ray	5.00	R	1-89	[»]
2HGP	X-ray	5.50	R	1-89	[»]
2HGR	X-ray	4.51	R	1-89	[»]
2HHH	X-ray	3.35	O	1-89	[»]
2J00	X-ray	2.80	O	2-88	[»]
2J02	X-ray	2.80	O	2-88	[»]
2OW8	X-ray	3.71	p	-	[»]
2UU9	X-ray	3.10	O	2-88	[»]
2UUA	X-ray	2.90	O	1-89	[»]
2UUB	X-ray	2.90	O	2-88	[»]
2UUC	X-ray	3.10	O	2-88	[»]
2UXB	X-ray	3.10	O	1-89	[»]
2UXC	X-ray	2.90	O	1-89	[»]
2UXD	X-ray	3.20	O	1-89	[»]
2V46	X-ray	3.80	O	2-88	[»]
2V48	X-ray	3.50	O	1-89	[»]
2VQE	X-ray	2.50	O	1-89	[»]
2VQF	X-ray	2.90	O	1-89	[»]
2WDG	X-ray	3.30	O	1-89	[»]
2WDH	X-ray	3.30	O	1-89	[»]
2WDK	X-ray	3.50	O	1-89	[»]
2WDM	X-ray	3.50	O	1-89	[»]
2WH1	X-ray	3.45	O	1-89	[»]
2WH3	X-ray	3.45	O	1-89	[»]
2WRI	X-ray	3.60	O	1-89	[»]
2WRK	X-ray	3.60	O	1-89	[»]
2WRN	X-ray	3.60	O	1-89	[»]
2WRQ	X-ray	3.60	O	1-89	[»]
2X9R	X-ray	3.10	O	1-89	[»]
2X9T	X-ray	3.10	O	1-89	[»]
2XFZ	X-ray	3.20	O	1-89	[»]
2XG1	X-ray	3.20	O	1-89	[»]
2XQD	X-ray	3.10	O	1-89	[»]
2XSY	electron microscopy	7.80	O	1-89	[»]
2XUY	electron microscopy	7.60	O	1-89	[»]
2Y0U	X-ray	3.10	O	1-89	[»]
2Y0W	X-ray	3.10	O	1-89	[»]
2Y0Y	X-ray	3.10	O	1-89	[»]
2Y10	X-ray	3.10	O	1-89	[»]
2Y12	X-ray	3.10	O	1-89	[»]
2Y14	X-ray	3.10	O	1-89	[»]
2Y16	X-ray	3.10	O	1-89	[»]
2Y18	X-ray	3.10	O	1-89	[»]
2ZM6	X-ray	3.30	O	2-89	[»]
3FIC	electron microscopy	6.40	O	2-89	[»]
3HUW	X-ray	3.10	O	1-89	[»]
3HUY	X-ray	3.10	O	1-89	[»]
3I8G	X-ray	3.10	R	1-89	[»]
3I8H	X-ray	3.10	R	1-89	[»]
3I9B	X-ray	3.50	R	1-89	[»]
3I9D	X-ray	3.50	R	1-89	[»]
3KIQ	X-ray	3.30	o	1-89	[»]
3KIS	X-ray	3.30	o	1-89	[»]
3KIU	X-ray	3.60	o	1-89	[»]
3KIX	X-ray	3.60	o	1-89	[»]
3KNH	X-ray	3.00	O	1-89	[»]
3KNJ	X-ray	3.15	O	1-89	[»]
3KNL	X-ray	3.45	O	1-89	[»]
3KNN	X-ray	3.45	O	1-89	[»]
3OGE	X-ray	3.00	O	1-89	[»]
3OGY	X-ray	3.00	O	1-89	[»]
3OHC	X-ray	3.00	O	1-89	[»]
3OHD	X-ray	3.00	O	1-89	[»]
3OHY	X-ray	3.00	O	1-89	[»]
3OI0	X-ray	3.00	O	1-89	[»]
3OI2	X-ray	3.10	O	1-89	[»]
3OI4	X-ray	3.10	O	1-89	[»]
3OTO	X-ray	3.69	O	1-89	[»]
3T1H	X-ray	3.11	O	1-89	[»]
3T1Y	X-ray	2.80	O	1-89	[»]
3TVF	X-ray	3.10	R	1-89	[»]
3TVG	X-ray	3.10	R	1-89	[»]
3UXS	X-ray	3.20	O	1-89	[»]
3UXT	X-ray	3.20	O	1-89	[»]
3UYD	X-ray	3.00	R	1-89	[»]
3UYF	X-ray	3.00	R	1-89	[»]
3UZ3	X-ray	3.30	R	1-89	[»]
3UZ4	X-ray	3.30	R	1-89	[»]
3UZ6	X-ray	3.00	R	1-89	[»]
3UZ7	X-ray	3.00	R	1-89	[»]
3UZG	X-ray	3.30	R	1-89	[»]
3UZI	X-ray	3.30	R	1-89	[»]
3UZL	X-ray	3.30	R	1-89	[»]
3UZM	X-ray	3.30	R	1-89	[»]
3V22	X-ray	3.00	O	1-89	[»]
3V24	X-ray	3.00	O	1-89	[»]
3V26	X-ray	3.10	O	1-89	[»]
3V28	X-ray	3.10	O	1-89	[»]
3V2C	X-ray	2.70	O	1-89	[»]
3V2E	X-ray	2.70	O	1-89	[»]
3ZVO	X-ray	3.80	O	1-89	[»]
4ABR	X-ray	3.10	O	1-89	[»]
4AQY	X-ray	3.50	O	2-89	[»]
4DH9	X-ray	3.20	O	1-89	[»]
4DHB	X-ray	3.20	O	1-89	[»]
4DR1	X-ray	3.60	O	1-89	[»]
4DR2	X-ray	3.25	O	1-89	[»]
4DR3	X-ray	3.35	O	1-89	[»]
4DR4	X-ray	3.97	O	1-89	[»]
4DR5	X-ray	3.45	O	1-89	[»]
4DR6	X-ray	3.30	O	1-89	[»]
4DR7	X-ray	3.75	O	1-89	[»]
4DUY	X-ray	3.39	O	1-89	[»]
4DUZ	X-ray	3.65	O	1-89	[»]
4DV0	X-ray	3.85	O	1-89	[»]
4DV1	X-ray	3.85	O	1-89	[»]
4DV2	X-ray	3.65	O	1-89	[»]
4DV3	X-ray	3.55	O	1-89	[»]
4DV4	X-ray	3.65	O	1-89	[»]
4DV5	X-ray	3.68	O	1-89	[»]
4DV6	X-ray	3.30	O	1-89	[»]
4DV7	X-ray	3.29	O	1-89	[»]
4G5K	X-ray	3.30	R	1-89	[»]
4G5M	X-ray	3.30	R	1-89	[»]
4G5T	X-ray	3.10	R	1-89	[»]
4G5V	X-ray	3.10	R	1-89	[»]

ProteinModelPortal

Q5SJ76.

SMR

Q5SJ76. Positions 2-89.

ModBase

Search...

Protein-protein interaction databases

STRING

300852.TTHA1138.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAD70961; BAD70961; BAD70961.

GeneID

3169463.

KEGG

ttj:TTHA1138.

PATRIC

23957235. VBITheThe93045_1117.

Phylogenomic databases

eggNOG

COG0184.

HOGENOM

HOG000040097.

KO

K02956.

OMA

LKMVSRR.

ProtClustDB

PRK05626.

Family and domain databases

Gene3D

1.10.287.10. 1 hit.

HAMAP

MF_01343_B. Ribosomal_S15_S13e_B.

InterPro

IPR000589. Ribosomal_S15.
IPR005290. Ribosomal_S15_bac-type.
IPR009068. S15_NS1_RNA-bd.
[Graphical view]

PANTHER

PTHR23321:SF10. PTHR23321:SF10. 1 hit.

Pfam

PF00312. Ribosomal_S15. 1 hit.
[Graphical view]

SUPFAM

SSF47060. S15/NS1_bind. 1 hit.

TIGRFAMs

TIGR00952. S15_bact. 1 hit.

PROSITE

PS00362. RIBOSOMAL_S15. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q5SJ76.

Entry information

Entry name

RS15_THET8

Accession

Primary (citable) accession number: Q5SJ76

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program