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Protein

Protein translocase subunit SecA

Gene

secA

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi99 – 106ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein translocase subunit SecAUniRule annotation
Gene namesi
Name:secAUniRule annotation
Ordered Locus Names:TTHA1251
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
  • Cytoplasm UniRule annotation

  • Note: Distribution is 50-50.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003210261 – 997Protein translocase subunit SecAAdd BLAST997

Interactioni

Subunit structurei

Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved. Monomer and homodimer.1 Publication

Protein-protein interaction databases

DIPiDIP-59815N.
STRINGi300852.TTHA1251.

Structurei

Secondary structure

1997
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 9Combined sources6
Helixi11 – 22Combined sources12
Helixi24 – 29Combined sources6
Helixi31 – 35Combined sources5
Helixi40 – 52Combined sources13
Helixi57 – 75Combined sources19
Helixi82 – 92Combined sources11
Beta strandi95 – 98Combined sources4
Helixi103 – 117Combined sources15
Beta strandi124 – 129Combined sources6
Helixi130 – 145Combined sources16
Turni146 – 148Combined sources3
Beta strandi151 – 153Combined sources3
Helixi160 – 167Combined sources8
Beta strandi169 – 175Combined sources7
Helixi176 – 186Combined sources11
Turni192 – 194Combined sources3
Beta strandi199 – 203Combined sources5
Beta strandi205 – 208Combined sources4
Helixi211 – 214Combined sources4
Beta strandi223 – 228Combined sources6
Helixi233 – 245Combined sources13
Beta strandi254 – 256Combined sources3
Helixi277 – 287Combined sources11
Helixi290 – 293Combined sources4
Turni294 – 296Combined sources3
Helixi298 – 312Combined sources15
Helixi316 – 319Combined sources4
Beta strandi320 – 322Combined sources3
Beta strandi327 – 331Combined sources5
Turni332 – 335Combined sources4
Helixi344 – 346Combined sources3
Helixi347 – 354Combined sources8
Beta strandi364 – 369Combined sources6
Helixi371 – 375Combined sources5
Beta strandi378 – 387Combined sources10
Helixi389 – 391Combined sources3
Helixi392 – 399Combined sources8
Beta strandi403 – 405Combined sources3
Beta strandi415 – 424Combined sources10
Helixi425 – 442Combined sources18
Beta strandi446 – 449Combined sources4
Helixi453 – 464Combined sources12
Helixi466 – 469Combined sources4
Helixi470 – 484Combined sources15
Helixi490 – 498Combined sources9
Beta strandi500 – 502Combined sources3
Helixi507 – 510Combined sources4
Helixi512 – 516Combined sources5
Helixi521 – 542Combined sources22
Beta strandi551 – 553Combined sources3
Helixi554 – 562Combined sources9
Turni563 – 565Combined sources3
Beta strandi570 – 573Combined sources4
Turni577 – 580Combined sources4
Helixi589 – 595Combined sources7
Beta strandi598 – 600Combined sources3
Helixi606 – 618Combined sources13
Helixi621 – 630Combined sources10
Helixi635 – 655Combined sources21
Turni656 – 658Combined sources3
Beta strandi663 – 667Combined sources5
Helixi672 – 680Combined sources9
Beta strandi690 – 699Combined sources10
Helixi700 – 704Combined sources5
Helixi710 – 716Combined sources7
Beta strandi721 – 723Combined sources3
Helixi728 – 774Combined sources47
Helixi778 – 801Combined sources24
Beta strandi807 – 809Combined sources3
Helixi812 – 821Combined sources10
Helixi830 – 836Combined sources7
Helixi840 – 860Combined sources21
Helixi863 – 896Combined sources34
Beta strandi901 – 904Combined sources4
Helixi906 – 934Combined sources29

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IPCX-ray2.80A/B/C/D1-997[»]
ProteinModelPortaliQ5SIW3.
SMRiQ5SIW3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SIW3.

Family & Domainsi

Sequence similaritiesi

Belongs to the SecA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CI6. Bacteria.
COG0653. LUCA.
HOGENOMiHOG000218168.
KOiK03070.
OMAiIATERHE.
PhylomeDBiQ5SIW3.

Family and domain databases

Gene3Di1.10.3060.10. 1 hit.
3.40.50.300. 4 hits.
3.90.1440.10. 1 hit.
HAMAPiMF_01382. SecA. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000185. SecA.
IPR020937. SecA_CS.
IPR011115. SecA_DEAD.
IPR014018. SecA_motor_DEAD.
IPR011130. SecA_preprotein_X-link_dom.
IPR011116. SecA_Wing/Scaffold.
[Graphical view]
PfamiPF07517. SecA_DEAD. 1 hit.
PF01043. SecA_PP_bind. 1 hit.
PF07516. SecA_SW. 1 hit.
[Graphical view]
PRINTSiPR00906. SECA.
SMARTiSM00957. SecA_DEAD. 1 hit.
SM00958. SecA_PP_bind. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 5 hits.
SSF81767. SSF81767. 1 hit.
SSF81886. SSF81886. 1 hit.
TIGRFAMsiTIGR00963. secA. 1 hit.
PROSITEiPS01312. SECA. 1 hit.
PS51196. SECA_MOTOR_DEAD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SIW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGLLRRLFD NNEREIARYY KQVVEPVNRL EAEVEKLPDL AAAYRELKEK
60 70 80 90 100
HEKGASLDEL LPMAFALTRE SAKRYLGMRH FDVQLIGGAV LHEGKIAEMK
110 120 130 140 150
TGEGKTLVAT LAVALNALTG KGVHVVTVND YLARRDAEWM GPVYRGLGLS
160 170 180 190 200
VGVIQHASTP AERRKAYLAD VTYVTNSELG FDYLRDNMAI SPDQLVLRHD
210 220 230 240 250
HPLHYAIIDE VDSILIDEAR TPLIISGPAE KATDLYYKMA EIAKKLERGL
260 270 280 290 300
PAEPGVRKEP TGDYTVEEKN RSVHLTLQGI AKAEKLLGIE GLFSPENMEL
310 320 330 340 350
AHMLIQAIRA KELYHRDRDY IVQDGQVIIV DEFTGRLMPG RRYGEGLHQA
360 370 380 390 400
IEAKEGVRIE RENQTLATIT YQNFFRLYEK RAGMTGTAKT EEKEFQEIYG
410 420 430 440 450
MDVVVVPTNR PVIRKDFPDV VYRTEKGKFY AVVEEIAEKY ERGQPVLVGT
460 470 480 490 500
ISIEKSERLS QMLKEPRLYL PRLEMRLELF KKASQKQQGP EWERLRKLLE
510 520 530 540 550
RPAQLKDEDL APFEGLIPPK GNLRTAWEGL KRAVHTLAVL RQGIPHQVLN
560 570 580 590 600
AKHHAREAEI VAQAGRSKTV TIATNMAGRG TDIKLGGNPE YLAAALLEKE
610 620 630 640 650
GFDRYEWKVE LFIKKMVAGK EEEARALAQE LGIREELLER IREIREECKQ
660 670 680 690 700
DEERVRALGG LFIIGTERHE SRRIDNQLRG RAGRQGDPGG SRFYVSFDDD
710 720 730 740 750
LMRLFASDRV IAMLDRMGFD DSEPIEHPMV TRSIERAQKR VEDRNFAIRK
760 770 780 790 800
QLLQFDDVLS RQREVIYAQR RLILLGKDEE VKEAAIGMVE ETVASLAENF
810 820 830 840 850
LNPEVHPEDW DLEGLKATLL DTAPQLQDFP FAELRALKAE EAVERLVEAA
860 870 880 890 900
LKAYEAREAE LSPPLMRAVE RFVILNVVDN AWKEHLHNLD VLRQGIFLRG
910 920 930 940 950
YGQKDPFQEY KIEATRLFNE MVAFIKSEVA KFLFRLKVEA EPVRPVREAP
960 970 980 990
YVPVPEAKPE PSEVFGVERK RATPPPQPGL SRAERRRLMR QEKKRKK
Length:997
Mass (Da):113,953
Last modified:December 21, 2004 - v1
Checksum:i9A22888B52698CCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71074.1.
RefSeqiWP_011228548.1. NC_006461.1.
YP_144517.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71074; BAD71074; BAD71074.
GeneIDi3169354.
KEGGittj:TTHA1251.
PATRICi23957465. VBITheThe93045_1230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71074.1.
RefSeqiWP_011228548.1. NC_006461.1.
YP_144517.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IPCX-ray2.80A/B/C/D1-997[»]
ProteinModelPortaliQ5SIW3.
SMRiQ5SIW3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59815N.
STRINGi300852.TTHA1251.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71074; BAD71074; BAD71074.
GeneIDi3169354.
KEGGittj:TTHA1251.
PATRICi23957465. VBITheThe93045_1230.

Phylogenomic databases

eggNOGiENOG4105CI6. Bacteria.
COG0653. LUCA.
HOGENOMiHOG000218168.
KOiK03070.
OMAiIATERHE.
PhylomeDBiQ5SIW3.

Miscellaneous databases

EvolutionaryTraceiQ5SIW3.

Family and domain databases

Gene3Di1.10.3060.10. 1 hit.
3.40.50.300. 4 hits.
3.90.1440.10. 1 hit.
HAMAPiMF_01382. SecA. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000185. SecA.
IPR020937. SecA_CS.
IPR011115. SecA_DEAD.
IPR014018. SecA_motor_DEAD.
IPR011130. SecA_preprotein_X-link_dom.
IPR011116. SecA_Wing/Scaffold.
[Graphical view]
PfamiPF07517. SecA_DEAD. 1 hit.
PF01043. SecA_PP_bind. 1 hit.
PF07516. SecA_SW. 1 hit.
[Graphical view]
PRINTSiPR00906. SECA.
SMARTiSM00957. SecA_DEAD. 1 hit.
SM00958. SecA_PP_bind. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 5 hits.
SSF81767. SSF81767. 1 hit.
SSF81886. SSF81886. 1 hit.
TIGRFAMsiTIGR00963. secA. 1 hit.
PROSITEiPS01312. SECA. 1 hit.
PS51196. SECA_MOTOR_DEAD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSECA_THET8
AccessioniPrimary (citable) accession number: Q5SIW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.