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Protein

Protein translocase subunit SecA

Gene

secA

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi99 – 1068ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1285-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein translocase subunit SecAUniRule annotation
Gene namesi
Name:secAUniRule annotation
Ordered Locus Names:TTHA1251
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
  • Cytoplasm UniRule annotation

  • Note: Distribution is 50-50.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 997997Protein translocase subunit SecAPRO_0000321026Add
BLAST

Interactioni

Subunit structurei

Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved. Monomer and homodimer.1 Publication

Protein-protein interaction databases

DIPiDIP-59815N.
STRINGi300852.TTHA1251.

Structurei

Secondary structure

1
997
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 96Combined sources
Helixi11 – 2212Combined sources
Helixi24 – 296Combined sources
Helixi31 – 355Combined sources
Helixi40 – 5213Combined sources
Helixi57 – 7519Combined sources
Helixi82 – 9211Combined sources
Beta strandi95 – 984Combined sources
Helixi103 – 11715Combined sources
Beta strandi124 – 1296Combined sources
Helixi130 – 14516Combined sources
Turni146 – 1483Combined sources
Beta strandi151 – 1533Combined sources
Helixi160 – 1678Combined sources
Beta strandi169 – 1757Combined sources
Helixi176 – 18611Combined sources
Turni192 – 1943Combined sources
Beta strandi199 – 2035Combined sources
Beta strandi205 – 2084Combined sources
Helixi211 – 2144Combined sources
Beta strandi223 – 2286Combined sources
Helixi233 – 24513Combined sources
Beta strandi254 – 2563Combined sources
Helixi277 – 28711Combined sources
Helixi290 – 2934Combined sources
Turni294 – 2963Combined sources
Helixi298 – 31215Combined sources
Helixi316 – 3194Combined sources
Beta strandi320 – 3223Combined sources
Beta strandi327 – 3315Combined sources
Turni332 – 3354Combined sources
Helixi344 – 3463Combined sources
Helixi347 – 3548Combined sources
Beta strandi364 – 3696Combined sources
Helixi371 – 3755Combined sources
Beta strandi378 – 38710Combined sources
Helixi389 – 3913Combined sources
Helixi392 – 3998Combined sources
Beta strandi403 – 4053Combined sources
Beta strandi415 – 42410Combined sources
Helixi425 – 44218Combined sources
Beta strandi446 – 4494Combined sources
Helixi453 – 46412Combined sources
Helixi466 – 4694Combined sources
Helixi470 – 48415Combined sources
Helixi490 – 4989Combined sources
Beta strandi500 – 5023Combined sources
Helixi507 – 5104Combined sources
Helixi512 – 5165Combined sources
Helixi521 – 54222Combined sources
Beta strandi551 – 5533Combined sources
Helixi554 – 5629Combined sources
Turni563 – 5653Combined sources
Beta strandi570 – 5734Combined sources
Turni577 – 5804Combined sources
Helixi589 – 5957Combined sources
Beta strandi598 – 6003Combined sources
Helixi606 – 61813Combined sources
Helixi621 – 63010Combined sources
Helixi635 – 65521Combined sources
Turni656 – 6583Combined sources
Beta strandi663 – 6675Combined sources
Helixi672 – 6809Combined sources
Beta strandi690 – 69910Combined sources
Helixi700 – 7045Combined sources
Helixi710 – 7167Combined sources
Beta strandi721 – 7233Combined sources
Helixi728 – 77447Combined sources
Helixi778 – 80124Combined sources
Beta strandi807 – 8093Combined sources
Helixi812 – 82110Combined sources
Helixi830 – 8367Combined sources
Helixi840 – 86021Combined sources
Helixi863 – 89634Combined sources
Beta strandi901 – 9044Combined sources
Helixi906 – 93429Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IPCX-ray2.80A/B/C/D1-997[»]
ProteinModelPortaliQ5SIW3.
SMRiQ5SIW3. Positions 1-939.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SIW3.

Family & Domainsi

Sequence similaritiesi

Belongs to the SecA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CI6. Bacteria.
COG0653. LUCA.
HOGENOMiHOG000218168.
KOiK03070.
OMAiIATERHE.
OrthoDBiEOG654P48.
PhylomeDBiQ5SIW3.

Family and domain databases

Gene3Di1.10.3060.10. 1 hit.
3.40.50.300. 4 hits.
3.90.1440.10. 1 hit.
HAMAPiMF_01382. SecA.
InterProiIPR027417. P-loop_NTPase.
IPR000185. SecA.
IPR020937. SecA_CS.
IPR011115. SecA_DEAD.
IPR014018. SecA_motor_DEAD.
IPR011130. SecA_preprotein_X-link_dom.
IPR011116. SecA_Wing/Scaffold.
[Graphical view]
PfamiPF07517. SecA_DEAD. 1 hit.
PF01043. SecA_PP_bind. 1 hit.
PF07516. SecA_SW. 1 hit.
[Graphical view]
PRINTSiPR00906. SECA.
SMARTiSM00957. SecA_DEAD. 1 hit.
SM00958. SecA_PP_bind. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 5 hits.
SSF81767. SSF81767. 1 hit.
SSF81886. SSF81886. 1 hit.
TIGRFAMsiTIGR00963. secA. 1 hit.
PROSITEiPS01312. SECA. 1 hit.
PS51196. SECA_MOTOR_DEAD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SIW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGLLRRLFD NNEREIARYY KQVVEPVNRL EAEVEKLPDL AAAYRELKEK
60 70 80 90 100
HEKGASLDEL LPMAFALTRE SAKRYLGMRH FDVQLIGGAV LHEGKIAEMK
110 120 130 140 150
TGEGKTLVAT LAVALNALTG KGVHVVTVND YLARRDAEWM GPVYRGLGLS
160 170 180 190 200
VGVIQHASTP AERRKAYLAD VTYVTNSELG FDYLRDNMAI SPDQLVLRHD
210 220 230 240 250
HPLHYAIIDE VDSILIDEAR TPLIISGPAE KATDLYYKMA EIAKKLERGL
260 270 280 290 300
PAEPGVRKEP TGDYTVEEKN RSVHLTLQGI AKAEKLLGIE GLFSPENMEL
310 320 330 340 350
AHMLIQAIRA KELYHRDRDY IVQDGQVIIV DEFTGRLMPG RRYGEGLHQA
360 370 380 390 400
IEAKEGVRIE RENQTLATIT YQNFFRLYEK RAGMTGTAKT EEKEFQEIYG
410 420 430 440 450
MDVVVVPTNR PVIRKDFPDV VYRTEKGKFY AVVEEIAEKY ERGQPVLVGT
460 470 480 490 500
ISIEKSERLS QMLKEPRLYL PRLEMRLELF KKASQKQQGP EWERLRKLLE
510 520 530 540 550
RPAQLKDEDL APFEGLIPPK GNLRTAWEGL KRAVHTLAVL RQGIPHQVLN
560 570 580 590 600
AKHHAREAEI VAQAGRSKTV TIATNMAGRG TDIKLGGNPE YLAAALLEKE
610 620 630 640 650
GFDRYEWKVE LFIKKMVAGK EEEARALAQE LGIREELLER IREIREECKQ
660 670 680 690 700
DEERVRALGG LFIIGTERHE SRRIDNQLRG RAGRQGDPGG SRFYVSFDDD
710 720 730 740 750
LMRLFASDRV IAMLDRMGFD DSEPIEHPMV TRSIERAQKR VEDRNFAIRK
760 770 780 790 800
QLLQFDDVLS RQREVIYAQR RLILLGKDEE VKEAAIGMVE ETVASLAENF
810 820 830 840 850
LNPEVHPEDW DLEGLKATLL DTAPQLQDFP FAELRALKAE EAVERLVEAA
860 870 880 890 900
LKAYEAREAE LSPPLMRAVE RFVILNVVDN AWKEHLHNLD VLRQGIFLRG
910 920 930 940 950
YGQKDPFQEY KIEATRLFNE MVAFIKSEVA KFLFRLKVEA EPVRPVREAP
960 970 980 990
YVPVPEAKPE PSEVFGVERK RATPPPQPGL SRAERRRLMR QEKKRKK
Length:997
Mass (Da):113,953
Last modified:December 21, 2004 - v1
Checksum:i9A22888B52698CCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71074.1.
RefSeqiWP_011228548.1. NC_006461.1.
YP_144517.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71074; BAD71074; BAD71074.
GeneIDi3169354.
KEGGittj:TTHA1251.
PATRICi23957465. VBITheThe93045_1230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71074.1.
RefSeqiWP_011228548.1. NC_006461.1.
YP_144517.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IPCX-ray2.80A/B/C/D1-997[»]
ProteinModelPortaliQ5SIW3.
SMRiQ5SIW3. Positions 1-939.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59815N.
STRINGi300852.TTHA1251.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71074; BAD71074; BAD71074.
GeneIDi3169354.
KEGGittj:TTHA1251.
PATRICi23957465. VBITheThe93045_1230.

Phylogenomic databases

eggNOGiENOG4105CI6. Bacteria.
COG0653. LUCA.
HOGENOMiHOG000218168.
KOiK03070.
OMAiIATERHE.
OrthoDBiEOG654P48.
PhylomeDBiQ5SIW3.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1285-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ5SIW3.

Family and domain databases

Gene3Di1.10.3060.10. 1 hit.
3.40.50.300. 4 hits.
3.90.1440.10. 1 hit.
HAMAPiMF_01382. SecA.
InterProiIPR027417. P-loop_NTPase.
IPR000185. SecA.
IPR020937. SecA_CS.
IPR011115. SecA_DEAD.
IPR014018. SecA_motor_DEAD.
IPR011130. SecA_preprotein_X-link_dom.
IPR011116. SecA_Wing/Scaffold.
[Graphical view]
PfamiPF07517. SecA_DEAD. 1 hit.
PF01043. SecA_PP_bind. 1 hit.
PF07516. SecA_SW. 1 hit.
[Graphical view]
PRINTSiPR00906. SECA.
SMARTiSM00957. SecA_DEAD. 1 hit.
SM00958. SecA_PP_bind. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 5 hits.
SSF81767. SSF81767. 1 hit.
SSF81886. SSF81886. 1 hit.
TIGRFAMsiTIGR00963. secA. 1 hit.
PROSITEiPS01312. SECA. 1 hit.
PS51196. SECA_MOTOR_DEAD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.
  2. "Conformational transition of Sec machinery inferred from bacterial SecYE structures."
    Tsukazaki T., Mori H., Fukai S., Ishitani R., Mori T., Dohmae N., Perederina A., Sugita Y., Vassylyev D.G., Ito K., Nureki O.
    Nature 455:988-991(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SECY.
  3. "Crystal structure of the translocation ATPase SecA from Thermus thermophilus reveals a parallel, head-to-head dimer."
    Vassylyev D.G., Mori H., Vassylyeva M.N., Tsukazaki T., Kimura Y., Tahirov T.H., Ito K.
    J. Mol. Biol. 364:248-258(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiSECA_THET8
AccessioniPrimary (citable) accession number: Q5SIW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: December 21, 2004
Last modified: November 11, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.