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Protein

4-hydroxy-4-methyl-2-oxoglutarate aldolase

Gene

TTHA1322

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions.1 Publication

Catalytic activityi

4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate.1 Publication
Oxaloacetate = pyruvate + CO2.1 Publication

Cofactori

Ni2+1 Publication, Co2+1 Publication, Zn2+1 PublicationNote: Divalent metal cation. Has preference for nickel and cobalt ions for the HMG aldolase activity. Has preference for zinc ions for the OAA decarboxylase activity.1 Publication

Enzyme regulationi

Competitively inhibited by oxalate, a pyruvate enolate analog.1 Publication

Kineticsi

kcat is 0.356 sec(-1) with 4-hydroxy-4-methyl-2-oxoglutarate as substrate and 1.06 sec(-1) with oxaloacetate as substrate.

  1. KM=150.7 µM for 4-hydroxy-4-methyl-2-oxoglutarate1 Publication
  2. KM=211.4 µM for oxaloacetate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei96 – 961SubstrateBy similarity
    Metal bindingi97 – 971Divalent metal cationBy similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Metal-binding

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-1356-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxy-4-methyl-2-oxoglutarate aldolase (EC:4.1.3.17)
    Short name:
    HMG aldolase
    Alternative name(s):
    Oxaloacetate decarboxylase (EC:4.1.1.3)
    Short name:
    OAA decarboxylase
    Regulator of ribonuclease activity homolog
    Gene namesi
    Ordered Locus Names:TTHA1322
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    Proteomesi
    • UP000000532 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 1641644-hydroxy-4-methyl-2-oxoglutarate aldolasePRO_1000013877Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer.1 Publication

    Protein-protein interaction databases

    STRINGi300852.TTHA1322.

    Structurei

    Secondary structure

    1
    164
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 117Combined sources
    Beta strandi15 – 184Combined sources
    Beta strandi22 – 243Combined sources
    Beta strandi26 – 3914Combined sources
    Beta strandi41 – 433Combined sources
    Helixi45 – 517Combined sources
    Beta strandi58 – 636Combined sources
    Beta strandi69 – 724Combined sources
    Helixi75 – 839Combined sources
    Beta strandi88 – 958Combined sources
    Helixi98 – 1014Combined sources
    Beta strandi104 – 11310Combined sources
    Beta strandi115 – 1173Combined sources
    Beta strandi125 – 1273Combined sources
    Beta strandi130 – 1323Combined sources
    Beta strandi135 – 1373Combined sources
    Beta strandi142 – 1465Combined sources
    Beta strandi149 – 1568Combined sources
    Beta strandi160 – 1623Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1J3LX-ray2.30A/B/C/D/E/F1-164[»]
    ProteinModelPortaliQ5SIP7.
    SMRiQ5SIP7. Positions 1-164.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ5SIP7.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni74 – 774Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class II aldolase/RraA-like family.Curated

    Phylogenomic databases

    eggNOGiENOG4108YYX. Bacteria.
    COG0684. LUCA.
    HOGENOMiHOG000252803.
    KOiK02553.
    OMAiEDNTLVR.
    PhylomeDBiQ5SIP7.

    Family and domain databases

    Gene3Di3.50.30.40. 1 hit.
    InterProiIPR010203. RraA.
    IPR005493. RraA/RraA-like.
    [Graphical view]
    PfamiPF03737. Methyltransf_6. 1 hit.
    [Graphical view]
    SUPFAMiSSF89562. SSF89562. 1 hit.
    TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5SIP7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEARTTDLSD LYPEGEALPM VFKSFGGRAR FAGRVRTLRV FEDNALVRKV
    60 70 80 90 100
    LEEEGAGQVL FVDGGGSLRT ALLGGNLARL AWEKGWAGVV VHGAVRDTEE
    110 120 130 140 150
    LREVPIGLLA LAATPKKSAK EGKGEVDVPL KVLGVEVLPG SFLLADEDGL
    160
    LLLPEPPSGV RSGG
    Length:164
    Mass (Da):17,345
    Last modified:December 21, 2004 - v1
    Checksum:i84F94FD9FDD952FA
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AP008226 Genomic DNA. Translation: BAD71145.1.
    RefSeqiWP_011228595.1. NC_006461.1.
    YP_144588.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD71145; BAD71145; BAD71145.
    GeneIDi3168568.
    KEGGittj:TTHA1322.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AP008226 Genomic DNA. Translation: BAD71145.1.
    RefSeqiWP_011228595.1. NC_006461.1.
    YP_144588.1. NC_006461.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1J3LX-ray2.30A/B/C/D/E/F1-164[»]
    ProteinModelPortaliQ5SIP7.
    SMRiQ5SIP7. Positions 1-164.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi300852.TTHA1322.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiBAD71145; BAD71145; BAD71145.
    GeneIDi3168568.
    KEGGittj:TTHA1322.

    Phylogenomic databases

    eggNOGiENOG4108YYX. Bacteria.
    COG0684. LUCA.
    HOGENOMiHOG000252803.
    KOiK02553.
    OMAiEDNTLVR.
    PhylomeDBiQ5SIP7.

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-1356-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiQ5SIP7.

    Family and domain databases

    Gene3Di3.50.30.40. 1 hit.
    InterProiIPR010203. RraA.
    IPR005493. RraA/RraA-like.
    [Graphical view]
    PfamiPF03737. Methyltransf_6. 1 hit.
    [Graphical view]
    SUPFAMiSSF89562. SSF89562. 1 hit.
    TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiRRAAH_THET8
    AccessioniPrimary (citable) accession number: Q5SIP7
    Secondary accession number(s): P83846
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: December 21, 2004
    Last modified: September 7, 2016
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.