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Protein

Endonuclease V

Gene

nfi

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi42 – 421MagnesiumUniRule annotation
Metal bindingi110 – 1101MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1381-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease VUniRule annotation (EC:3.1.21.7UniRule annotation)
Alternative name(s):
Deoxyinosine 3'endonucleaseUniRule annotation
Deoxyribonuclease VUniRule annotation
Short name:
DNase VUniRule annotation
Gene namesi
Name:nfiUniRule annotation
Ordered Locus Names:TTHA1347
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 226226Endonuclease VPRO_0000159676Add
BLAST

Interactioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei80 – 801Interaction with target DNAUniRule annotation

Protein-protein interaction databases

STRINGi300852.TTHA1347.

Structurei

3D structure databases

ProteinModelPortaliQ5SIM2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the endonuclease V family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105Y7X. Bacteria.
COG1515. LUCA.
HOGENOMiHOG000229135.
KOiK05982.
OMAiSYKGNKA.
PhylomeDBiQ5SIM2.

Family and domain databases

CDDicd06559. Endonuclease_V. 1 hit.
HAMAPiMF_00801. Endonuclease_5. 1 hit.
InterProiIPR007581. Endonuclease-V.
[Graphical view]
PfamiPF04493. Endonuclease_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SIM2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPPPFPKPA HPEEALALQR ALAKKVRLAG SLEGVRRIAA LDASHKRGRP
60 70 80 90 100
LVAVALLYDL EKGPLHVATA LLPEEALFPY VPGLLSFREA PAYLEALAAL
110 120 130 140 150
PEAPEALLVD GQGVAHPRGL GIASHLGVHL DLPSVGVAKR LLYGRPEAPL
160 170 180 190 200
PEEKGAAVRL LAPDGRPLGY VYRSRTGVKP LYVSPGHRVG LEEALRFVRR
210 220
LPTRFRLPEP LRLAHLEAGR ALKALD
Length:226
Mass (Da):24,431
Last modified:December 21, 2004 - v1
Checksum:i64E55AC96B507DCC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71170.1.
RefSeqiWP_011173403.1. NC_006461.1.
YP_144613.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71170; BAD71170; BAD71170.
GeneIDi3168384.
KEGGittj:TTHA1347.
PATRICi23957653. VBITheThe93045_1324.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71170.1.
RefSeqiWP_011173403.1. NC_006461.1.
YP_144613.1. NC_006461.1.

3D structure databases

ProteinModelPortaliQ5SIM2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1347.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71170; BAD71170; BAD71170.
GeneIDi3168384.
KEGGittj:TTHA1347.
PATRICi23957653. VBITheThe93045_1324.

Phylogenomic databases

eggNOGiENOG4105Y7X. Bacteria.
COG1515. LUCA.
HOGENOMiHOG000229135.
KOiK05982.
OMAiSYKGNKA.
PhylomeDBiQ5SIM2.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1381-MONOMER.

Family and domain databases

CDDicd06559. Endonuclease_V. 1 hit.
HAMAPiMF_00801. Endonuclease_5. 1 hit.
InterProiIPR007581. Endonuclease-V.
[Graphical view]
PfamiPF04493. Endonuclease_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNFI_THET8
AccessioniPrimary (citable) accession number: Q5SIM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: December 21, 2004
Last modified: September 7, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.