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Protein

Transcriptional regulator SdrP

Gene

TTHA1359

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates transcription. The consensus DNA-binding site of this transcriptional regulator is 5'-WWGTGAN5-7ACACWW-3' in which W is A or T and N is G, A, T or C. Regulated genes include those encoding proteins involved in nutrient and energy supply, redox control and polyadenylation of mRNA (PubMed:18699868). Also regulates genes involved in oxidative stress response such as genes encoding manganese superoxide dismutase and catalase, and genes encoding a protein involved in nucleotide excision repair of damaged DNA and putative proteins involved in redox control, protein degradation and transcriptional regulation (PubMed:21054499).2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi149 – 168H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding Source: UniProtKB
  • DNA binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • response to oxidative stress Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1348-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulator SdrP1 Publication
Alternative name(s):
Cyclic AMP receptor protein/Fumarate and nitrate reduction regulator superfamily protein SdrP1 Publication
Short name:
CRP/FNR superfamily protein SdrP1 Publication
Oxidative stress-responsive transcriptional activator SdrP1 Publication
Stationary phase-dependent regulatory protein1 Publication
Short name:
SdrP1 Publication
Gene namesi
Ordered Locus Names:TTHA1359Imported
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)Imported
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Viable, but has growth defects, particularly when grown in a synthetic medium. Increased sensitivity to disulfide stress. Decreased expression of TTHA0986, TTHA0770, TTHA0337, TTHA1028, TTHA0654, TTHA0655, TTHA0769, TTHA0425, TTHA0634, TTHA0635, TTHA0636, TTHA0637, TTHA0638, TTHA0570, TTHA0030, TTHA0460, TTHB243, TTHA1128, TTHA0035, TTHA0520 and TTHA1803 genes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00004362592 – 202Transcriptional regulator SdrPAdd BLAST201

Expressioni

Inductioni

Increased expression during the stationary phase when grown at 70 degrees Celsius (PubMed:18699868). Increased expression during the logarithmic growth phase in oxidative stress and upon treatment with diamide. Increased expression by heavy metal ion, antibiotic, high salt and organic solvent stresses (PubMed:21054499).2 Publications

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

STRINGi300852.TTHA1359.

Structurei

Secondary structure

1202
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 10Combined sources3
Beta strandi15 – 17Combined sources3
Beta strandi19 – 21Combined sources3
Beta strandi29 – 34Combined sources6
Beta strandi36 – 41Combined sources6
Beta strandi47 – 53Combined sources7
Helixi61 – 64Combined sources4
Beta strandi71 – 77Combined sources7
Beta strandi79 – 83Combined sources5
Helixi90 – 116Combined sources27
Helixi119 – 130Combined sources12
Beta strandi136 – 139Combined sources4
Beta strandi142 – 146Combined sources5
Helixi149 – 156Combined sources8
Helixi160 – 172Combined sources13
Beta strandi175 – 179Combined sources5
Beta strandi182 – 186Combined sources5
Helixi188 – 195Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZCWX-ray1.50A1-202[»]
ProteinModelPortaliQ5SIL0.
SMRiQ5SIL0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SIL0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini117 – 189HTH crp-typePROSITE-ProRule annotationAdd BLAST73

Sequence similaritiesi

Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108W63. Bacteria.
COG0664. LUCA.
HOGENOMiHOG000250566.
OMAiNAEDNLE.
PhylomeDBiQ5SIL0.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP.
IPR014710. RmlC-like_jellyroll.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF13545. HTH_Crp_2. 1 hit.
[Graphical view]
SMARTiSM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5SIL0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQVRETVSF KAGDVILYPG VPGPRDRAYR VLEGLVRLEA VDEEGNALTL
60 70 80 90 100
RLVRPGGFFG EEALFGQERI YFAEAATDVR LEPLPENPDP ELLKDLAQHL
110 120 130 140 150
SQGLAEAYRR IERLATQRLK NRMAAALLEL SETPLAHEEE GKVVLKATHD
160 170 180 190 200
ELAAAVGSVR ETVTKVIGEL AREGYIRSGY GKIQLLDLKG LKELAESRGQ

GR
Length:202
Mass (Da):22,317
Last modified:December 21, 2004 - v1
Checksum:i5B2D974220CAFFD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71182.1.
RefSeqiWP_011173413.1. NC_006461.1.
YP_144625.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71182; BAD71182; BAD71182.
GeneIDi3169114.
KEGGittj:TTHA1359.
PATRICi23957677. VBITheThe93045_1336.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71182.1.
RefSeqiWP_011173413.1. NC_006461.1.
YP_144625.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZCWX-ray1.50A1-202[»]
ProteinModelPortaliQ5SIL0.
SMRiQ5SIL0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1359.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71182; BAD71182; BAD71182.
GeneIDi3169114.
KEGGittj:TTHA1359.
PATRICi23957677. VBITheThe93045_1336.

Phylogenomic databases

eggNOGiENOG4108W63. Bacteria.
COG0664. LUCA.
HOGENOMiHOG000250566.
OMAiNAEDNLE.
PhylomeDBiQ5SIL0.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1348-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ5SIL0.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP.
IPR014710. RmlC-like_jellyroll.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF13545. HTH_Crp_2. 1 hit.
[Graphical view]
SMARTiSM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSDRP_THET8
AccessioniPrimary (citable) accession number: Q5SIL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 11, 2016
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.