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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 13FADUniRule annotation6

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Ordered Locus Names:TTHA1442
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003464201 – 443Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOAdd BLAST443

Proteomic databases

PRIDEiQ5SID2.

Interactioni

Protein-protein interaction databases

STRINGi300852.TTHA1442.

Structurei

Secondary structure

1443
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 7Combined sources4
Helixi11 – 22Combined sources12
Beta strandi27 – 30Combined sources4
Turni33 – 35Combined sources3
Beta strandi54 – 57Combined sources4
Helixi63 – 74Combined sources12
Helixi77 – 84Combined sources8
Beta strandi85 – 87Combined sources3
Beta strandi92 – 95Combined sources4
Helixi97 – 109Combined sources13
Beta strandi114 – 117Combined sources4
Beta strandi126 – 131Combined sources6
Helixi139 – 149Combined sources11
Helixi151 – 153Combined sources3
Beta strandi154 – 160Combined sources7
Beta strandi163 – 165Combined sources3
Helixi166 – 168Combined sources3
Helixi171 – 173Combined sources3
Beta strandi187 – 191Combined sources5
Helixi193 – 205Combined sources13
Helixi226 – 231Combined sources6
Helixi236 – 239Combined sources4
Turni240 – 242Combined sources3
Turni250 – 252Combined sources3
Beta strandi257 – 264Combined sources8
Beta strandi271 – 274Combined sources4
Helixi283 – 290Combined sources8
Beta strandi301 – 303Combined sources3
Beta strandi306 – 313Combined sources8
Helixi315 – 318Combined sources4
Beta strandi323 – 325Combined sources3
Beta strandi328 – 333Combined sources6
Helixi335 – 338Combined sources4
Helixi343 – 361Combined sources19
Helixi374 – 384Combined sources11
Helixi397 – 399Combined sources3
Beta strandi405 – 407Combined sources3
Helixi409 – 429Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3G5QX-ray2.10A1-443[»]
3G5RX-ray1.60A1-443[»]
3G5SX-ray1.05A1-443[»]
ProteinModelPortaliQ5SID2.
SMRiQ5SID2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SID2.

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
PhylomeDBiQ5SID2.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.50.50.60. 2 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR016040. NAD(P)-bd_dom.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SID2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERVNVVGAG LAGSEAAWTL LRLGVPVRLF EMRPKRMTPA HGTDRFAEIV
60 70 80 90 100
CSNSLGGEGE TNAKGLLQAE MRRAGSLVME AADLARVPAG GALAVDREEF
110 120 130 140 150
SGYITERLTG HPLLEVVREE VREIPPGITV LATGPLTSEA LAEALKRRFG
160 170 180 190 200
DHFLAYYDAA SPIVLYESID LTKCFRAGRY GQSADYLNCP MTEEEYRRFH
210 220 230 240 250
QALLEAQRHT PHDWEKLEFF EACVPVEELA RRGYQTLLFG PMKPVGLVDP
260 270 280 290 300
RTGKEPFAVV QLRQEDKAGR MWSLVGFQTG LKWPEQKRLI QMIPGLENAE
310 320 330 340 350
IVRYGVMHRN TYLNAPRLLG ETLEFREAEG LYAAGVLAGV EGYLESAATG
360 370 380 390 400
FLAGLNAARK ALGLPPVAPP EESMLGGLVR YLATANPEGF QPMYANWGLV
410 420 430 440
PPVEGRMGKK EKRQAMYRRG LEAFSAWLSG LNPPLPRPEA ALV
Length:443
Mass (Da):48,863
Last modified:December 21, 2004 - v1
Checksum:iC3D2B7B800C4A107
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71265.1.
RefSeqiWP_011228685.1. NC_006461.1.
YP_144708.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71265; BAD71265; BAD71265.
GeneIDi3169920.
KEGGittj:TTHA1442.
PATRICi23957841. VBITheThe93045_1416.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71265.1.
RefSeqiWP_011228685.1. NC_006461.1.
YP_144708.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3G5QX-ray2.10A1-443[»]
3G5RX-ray1.60A1-443[»]
3G5SX-ray1.05A1-443[»]
ProteinModelPortaliQ5SID2.
SMRiQ5SID2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1442.

Proteomic databases

PRIDEiQ5SID2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71265; BAD71265; BAD71265.
GeneIDi3169920.
KEGGittj:TTHA1442.
PATRICi23957841. VBITheThe93045_1416.

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
PhylomeDBiQ5SID2.

Miscellaneous databases

EvolutionaryTraceiQ5SID2.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.50.50.60. 2 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR016040. NAD(P)-bd_dom.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMFO_THET8
AccessioniPrimary (citable) accession number: Q5SID2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.