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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 136FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1478-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Ordered Locus Names:TTHA1442
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 443443Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_0000346420Add
BLAST

Proteomic databases

PRIDEiQ5SID2.

Interactioni

Protein-protein interaction databases

STRINGi300852.TTHA1442.

Structurei

Secondary structure

1
443
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 74Combined sources
Helixi11 – 2212Combined sources
Beta strandi27 – 304Combined sources
Turni33 – 353Combined sources
Beta strandi54 – 574Combined sources
Helixi63 – 7412Combined sources
Helixi77 – 848Combined sources
Beta strandi85 – 873Combined sources
Beta strandi92 – 954Combined sources
Helixi97 – 10913Combined sources
Beta strandi114 – 1174Combined sources
Beta strandi126 – 1316Combined sources
Helixi139 – 14911Combined sources
Helixi151 – 1533Combined sources
Beta strandi154 – 1607Combined sources
Beta strandi163 – 1653Combined sources
Helixi166 – 1683Combined sources
Helixi171 – 1733Combined sources
Beta strandi187 – 1915Combined sources
Helixi193 – 20513Combined sources
Helixi226 – 2316Combined sources
Helixi236 – 2394Combined sources
Turni240 – 2423Combined sources
Turni250 – 2523Combined sources
Beta strandi257 – 2648Combined sources
Beta strandi271 – 2744Combined sources
Helixi283 – 2908Combined sources
Beta strandi301 – 3033Combined sources
Beta strandi306 – 3138Combined sources
Helixi315 – 3184Combined sources
Beta strandi323 – 3253Combined sources
Beta strandi328 – 3336Combined sources
Helixi335 – 3384Combined sources
Helixi343 – 36119Combined sources
Helixi374 – 38411Combined sources
Helixi397 – 3993Combined sources
Beta strandi405 – 4073Combined sources
Helixi409 – 42921Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3G5QX-ray2.10A1-443[»]
3G5RX-ray1.60A1-443[»]
3G5SX-ray1.05A1-443[»]
ProteinModelPortaliQ5SID2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SID2.

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.
PhylomeDBiQ5SID2.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.50.50.60. 2 hits.
HAMAPiMF_01037. TrmFO.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR016040. NAD(P)-bd_dom.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SID2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERVNVVGAG LAGSEAAWTL LRLGVPVRLF EMRPKRMTPA HGTDRFAEIV
60 70 80 90 100
CSNSLGGEGE TNAKGLLQAE MRRAGSLVME AADLARVPAG GALAVDREEF
110 120 130 140 150
SGYITERLTG HPLLEVVREE VREIPPGITV LATGPLTSEA LAEALKRRFG
160 170 180 190 200
DHFLAYYDAA SPIVLYESID LTKCFRAGRY GQSADYLNCP MTEEEYRRFH
210 220 230 240 250
QALLEAQRHT PHDWEKLEFF EACVPVEELA RRGYQTLLFG PMKPVGLVDP
260 270 280 290 300
RTGKEPFAVV QLRQEDKAGR MWSLVGFQTG LKWPEQKRLI QMIPGLENAE
310 320 330 340 350
IVRYGVMHRN TYLNAPRLLG ETLEFREAEG LYAAGVLAGV EGYLESAATG
360 370 380 390 400
FLAGLNAARK ALGLPPVAPP EESMLGGLVR YLATANPEGF QPMYANWGLV
410 420 430 440
PPVEGRMGKK EKRQAMYRRG LEAFSAWLSG LNPPLPRPEA ALV
Length:443
Mass (Da):48,863
Last modified:December 21, 2004 - v1
Checksum:iC3D2B7B800C4A107
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71265.1.
RefSeqiWP_011228685.1. NC_006461.1.
YP_144708.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71265; BAD71265; BAD71265.
GeneIDi3169920.
KEGGittj:TTHA1442.
PATRICi23957841. VBITheThe93045_1416.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71265.1.
RefSeqiWP_011228685.1. NC_006461.1.
YP_144708.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3G5QX-ray2.10A1-443[»]
3G5RX-ray1.60A1-443[»]
3G5SX-ray1.05A1-443[»]
ProteinModelPortaliQ5SID2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1442.

Proteomic databases

PRIDEiQ5SID2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71265; BAD71265; BAD71265.
GeneIDi3169920.
KEGGittj:TTHA1442.
PATRICi23957841. VBITheThe93045_1416.

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.
PhylomeDBiQ5SID2.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1478-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ5SID2.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.50.50.60. 2 hits.
HAMAPiMF_01037. TrmFO.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR016040. NAD(P)-bd_dom.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiTRMFO_THET8
AccessioniPrimary (citable) accession number: Q5SID2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: December 21, 2004
Last modified: January 20, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.