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Q5SIA8 (PYRG_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
CTP synthase
EC=6.3.4.2
Alternative name(s):
CTP synthetase
UTP--ammonia ligase
Gene names
Name:pyrG
Ordered Locus Names:TTHA1466
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length550 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen By similarity. HAMAP MF_01227

Catalytic activity

ATP + UTP + NH3 = ADP + phosphate + CTP. HAMAP MF_01227

Enzyme regulation

Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP By similarity. HAMAP MF_01227

Pathway

Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. HAMAP MF_01227

Subunit structure

Homotetramer By similarity. HAMAP MF_01227

Sequence similarities

Belongs to the CTP synthase family.

Contains 1 glutamine amidotransferase type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 550550CTP synthase HAMAP MF_01227
PRO_0000266251

Regions

Domain302 – 549248Glutamine amidotransferase type-1
Region1 – 264264Aminator domain HAMAP MF_01227

Sites

Active site3911Nucleophile By similarity
Active site5221 By similarity
Active site5241 By similarity

Secondary structure

1 ........................................................................................... 550
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q5SIA8 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: F3D20D9FC50DFDF8

FASTA55061,003
        10         20         30         40         50         60 
MNGSADAGPR PRKYVFITGG VVSSLGKGIL TSSLGALLRA RGYRVTAIKI DPYVNVDAGT 

        70         80         90        100        110        120 
MRPYEHGEVF VTADGAETDL DIGHYERFLD MDLSRGNNLT TGQVYLSVIQ KERRGEYLSQ 

       130        140        150        160        170        180 
TVQVIPHITD EIKERIRKVA EEQKAEIVVV EVGGTVGDIE SLPFLEAIRQ FRFDEGEGNT 

       190        200        210        220        230        240 
LYLHLTLVPY LETSEEFKTK PTQHSVATLR GVGIQPDILV LRSARPVPEE VRRKVALFTN 

       250        260        270        280        290        300 
VRPGHVFSSP TVEHLYEVPL LLEEQGLGRA VERALGLEAV IPNLSFWQEA VRVLKHPERT 

       310        320        330        340        350        360 
VKIAIAGKYV KMPDAYLSLL EALRHAGIKN RARVEVKWVD AESLEAADLD EAFRDVSGIL 

       370        380        390        400        410        420 
VPGGFGVRGI EGKVRAAQYA RERKIPYLGI CLGLQIAVIE FARNVAGLKG ANSTEFDPHT 

       430        440        450        460        470        480 
PHPVIDLMPE QLEVEGLGGT MRLGDWPMRI KPGTLLHRLY GKEEVLERHR HRYEVNPLYV 

       490        500        510        520        530        540 
DGLERAGLVV SATTPGMRGR GAGLVEAIEL KDHPFFLGLQ SHPEFKSRPM RPSPPFVGFV 

       550 
EAALAYQERA

« Hide

References

[1]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP008226 Genomic DNA. Translation: BAD71289.1.
RefSeqYP_144732.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1VCMX-ray2.35A1-550[»]
1VCNX-ray2.25A1-550[»]
1VCOX-ray2.15A1-550[»]
ProteinModelPortalQ5SIA8.
SMRQ5SIA8. Positions 10-548.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ5SIA8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3169502.
GenomeReviewsGene locus TTHA1466 in contig AP008226_GR.
KEGGttj:TTHA1466.
NMPDRfig|300852.3.peg.1777.
PATRIC23957889. VBITheThe93045_1440.

Phylogenomic databases

eggNOGCOG0504.
HOGENOMHBG597806.
OMARVTMQKL.
PhylomeDBQ5SIA8.
ProtClustDBPRK05380.

Enzyme and pathway databases

BioCycTTHE300852:TTHA1466-MONOMER.

Family and domain databases

HAMAPMF_01227. PyrG.
[Tree]
InterProIPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE_1.
[Graphical view]
KOK01937.
PANTHERPTHR11550. PyrG_synth. 1 hit.
PfamPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00337. PyrG. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRG_THET8
AccessionPrimary (citable) accession number: Q5SIA8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 21, 2004
Last modified: January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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