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Protein

CTP synthase

Gene

pyrG

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.UniRule annotation

Catalytic activityi

ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.UniRule annotation

Enzyme regulationi

Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP.UniRule annotation

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes CTP from UDP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. CTP synthase (pyrG)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from UDP, the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei391 – 3911NucleophileUniRule annotation
Active sitei522 – 5221UniRule annotation
Active sitei524 – 5241UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1502-MONOMER.
BRENDAi6.3.4.2. 7852.
UniPathwayiUPA00159; UER00277.

Protein family/group databases

MEROPSiC26.964.

Names & Taxonomyi

Protein namesi
Recommended name:
CTP synthaseUniRule annotation (EC:6.3.4.2UniRule annotation)
Alternative name(s):
CTP synthetaseUniRule annotation
UTP--ammonia ligaseUniRule annotation
Gene namesi
Name:pyrGUniRule annotation
Ordered Locus Names:TTHA1466
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 550550CTP synthasePRO_0000266251Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi300852.TTHA1466.

Structurei

Secondary structure

1
550
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi13 – 197Combined sources
Beta strandi21 – 233Combined sources
Helixi27 – 3913Combined sources
Turni40 – 423Combined sources
Beta strandi45 – 517Combined sources
Helixi58 – 603Combined sources
Helixi81 – 899Combined sources
Helixi95 – 973Combined sources
Beta strandi98 – 1003Combined sources
Helixi101 – 11313Combined sources
Turni114 – 1196Combined sources
Turni124 – 1263Combined sources
Helixi127 – 14216Combined sources
Beta strandi146 – 1527Combined sources
Helixi159 – 1613Combined sources
Helixi162 – 1698Combined sources
Helixi171 – 1755Combined sources
Beta strandi179 – 1879Combined sources
Turni192 – 1954Combined sources
Helixi200 – 21112Combined sources
Beta strandi217 – 2259Combined sources
Helixi229 – 23911Combined sources
Helixi243 – 2453Combined sources
Beta strandi246 – 2505Combined sources
Helixi257 – 2659Combined sources
Helixi267 – 2748Combined sources
Helixi285 – 29511Combined sources
Beta strandi298 – 30811Combined sources
Helixi314 – 3163Combined sources
Helixi317 – 32913Combined sources
Beta strandi332 – 3409Combined sources
Helixi341 – 3433Combined sources
Helixi349 – 3524Combined sources
Turni353 – 3553Combined sources
Beta strandi359 – 3613Combined sources
Helixi370 – 38213Combined sources
Beta strandi387 – 3904Combined sources
Helixi392 – 40413Combined sources
Turni414 – 4163Combined sources
Beta strandi423 – 4286Combined sources
Helixi430 – 4323Combined sources
Beta strandi442 – 4509Combined sources
Helixi455 – 4606Combined sources
Beta strandi463 – 47513Combined sources
Helixi477 – 48610Combined sources
Beta strandi488 – 4936Combined sources
Beta strandi497 – 5015Combined sources
Beta strandi505 – 5106Combined sources
Beta strandi513 – 5219Combined sources
Helixi523 – 5264Combined sources
Beta strandi529 – 5313Combined sources
Helixi534 – 54613Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VCMX-ray2.35A1-550[»]
1VCNX-ray2.25A1-550[»]
1VCOX-ray2.15A1-550[»]
ProteinModelPortaliQ5SIA8.
SMRiQ5SIA8. Positions 10-548.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SIA8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini302 – 549248Glutamine amidotransferase type-1UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 264264Aminator domainAdd
BLAST

Sequence similaritiesi

Belongs to the CTP synthase family.UniRule annotation
Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105C8D. Bacteria.
COG0504. LUCA.
HOGENOMiHOG000077515.
KOiK01937.
OMAiMRLGEYE.
OrthoDBiEOG6RC3NR.
PhylomeDBiQ5SIA8.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_01227. PyrG.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SIA8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGSADAGPR PRKYVFITGG VVSSLGKGIL TSSLGALLRA RGYRVTAIKI
60 70 80 90 100
DPYVNVDAGT MRPYEHGEVF VTADGAETDL DIGHYERFLD MDLSRGNNLT
110 120 130 140 150
TGQVYLSVIQ KERRGEYLSQ TVQVIPHITD EIKERIRKVA EEQKAEIVVV
160 170 180 190 200
EVGGTVGDIE SLPFLEAIRQ FRFDEGEGNT LYLHLTLVPY LETSEEFKTK
210 220 230 240 250
PTQHSVATLR GVGIQPDILV LRSARPVPEE VRRKVALFTN VRPGHVFSSP
260 270 280 290 300
TVEHLYEVPL LLEEQGLGRA VERALGLEAV IPNLSFWQEA VRVLKHPERT
310 320 330 340 350
VKIAIAGKYV KMPDAYLSLL EALRHAGIKN RARVEVKWVD AESLEAADLD
360 370 380 390 400
EAFRDVSGIL VPGGFGVRGI EGKVRAAQYA RERKIPYLGI CLGLQIAVIE
410 420 430 440 450
FARNVAGLKG ANSTEFDPHT PHPVIDLMPE QLEVEGLGGT MRLGDWPMRI
460 470 480 490 500
KPGTLLHRLY GKEEVLERHR HRYEVNPLYV DGLERAGLVV SATTPGMRGR
510 520 530 540 550
GAGLVEAIEL KDHPFFLGLQ SHPEFKSRPM RPSPPFVGFV EAALAYQERA
Length:550
Mass (Da):61,003
Last modified:December 21, 2004 - v1
Checksum:iF3D20D9FC50DFDF8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71289.1.
RefSeqiWP_011228700.1. NC_006461.1.
YP_144732.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71289; BAD71289; BAD71289.
GeneIDi3169502.
KEGGittj:TTHA1466.
PATRICi23957889. VBITheThe93045_1440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71289.1.
RefSeqiWP_011228700.1. NC_006461.1.
YP_144732.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VCMX-ray2.35A1-550[»]
1VCNX-ray2.25A1-550[»]
1VCOX-ray2.15A1-550[»]
ProteinModelPortaliQ5SIA8.
SMRiQ5SIA8. Positions 10-548.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1466.

Protein family/group databases

MEROPSiC26.964.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71289; BAD71289; BAD71289.
GeneIDi3169502.
KEGGittj:TTHA1466.
PATRICi23957889. VBITheThe93045_1440.

Phylogenomic databases

eggNOGiENOG4105C8D. Bacteria.
COG0504. LUCA.
HOGENOMiHOG000077515.
KOiK01937.
OMAiMRLGEYE.
OrthoDBiEOG6RC3NR.
PhylomeDBiQ5SIA8.

Enzyme and pathway databases

UniPathwayiUPA00159; UER00277.
BioCyciTTHE300852:GH8R-1502-MONOMER.
BRENDAi6.3.4.2. 7852.

Miscellaneous databases

EvolutionaryTraceiQ5SIA8.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_01227. PyrG.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiPYRG_THET8
AccessioniPrimary (citable) accession number: Q5SIA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 21, 2004
Last modified: November 11, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.