Q5SI29 (LUXS_THET8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-ribosylhomocysteine lyase
EC=4.4.1.21

Alternative name(s):
AI-2 synthesis protein
Autoinducer-2 production protein LuxS

Gene names

Name:	luxS
Ordered Locus Names:	TTHA1551

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD) By similarity. HAMAP MF_00091
Catalytic activity	S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione. HAMAP MF_00091
Cofactor	Binds 1 iron ion per subunit By similarity. HAMAP MF_00091
Subunit structure	Homodimer By similarity. HAMAP MF_00091
Sequence similarities	Belongs to the LuxS family.

Ontologies

Keywords
Biological process	Autoinducer synthesis Quorum sensing
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	quorum sensing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	S-ribosylhomocysteine lyase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 155	155	S-ribosylhomocysteine lyase HAMAP MF_00091		PRO_0000298054
Sites
	Metal binding	53	1	Iron By similarity
	Metal binding	57	1	Iron By similarity
	Metal binding	121	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5SI29 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: FAB8FA5818E7E9BA	FASTA	155	16,823
	10 20 30 40 50 60 MAEVESFSLD HTKVKAPYVR LAGRKALAGG VVEKYDLRLA QPNREALPTG ALHTLEHLLA 70 80 90 100 110 120 GYLRDHLPGV IDLSPMGCRT GFYLVVEGPV GEEKVLEAFA QALKDVLAHE GEVPGASFRE 130 140 150 CGNYRDHDLP GAKAWAEKVL KAGLRVQATV PLEAR

References

[1]

"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008226 Genomic DNA. Translation: BAD71374.1.
RefSeq	YP_144817.1. NC_006461.1.
3D structure databases
HSSP	HSSP built from PDB template 1VH2 based on UniProtKB Q9RRU8.
ProteinModelPortal	Q5SI29.
SMR	Q5SI29. Positions 4-153.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5SI29.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3167928.
GenomeReviews	Gene locus TTHA1551 in contig AP008226_GR.
KEGG	ttj:TTHA1551.
PATRIC	23958059. VBITheThe93045_1522.
Phylogenomic databases
eggNOG	COG1854.
HOGENOM	HBG347473.
OMA	PHGDTIT.
ProtClustDB	PRK02260.
Enzyme and pathway databases
BioCyc	TTHE300852:TTHA1551-MONOMER.
Family and domain databases
HAMAP	MF_00091. LuxS. [Tree]
InterPro	IPR011249. Metalloenz_metal-bd. IPR003815. S-ribosylhomocysteinase. [Graphical view]
Gene3D	G3DSA:3.30.1360.80. S-ribosylhomocysteinase. 1 hit.
KO	K07173.
Pfam	PF02664. LuxS. 1 hit. [Graphical view]
PIRSF	PIRSF006160. AI2. 1 hit.
PRINTS	PR01487. LUXSPROTEIN.
ProDom	PD013172. S-ribosylhomocysteinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF63411. Metalloenz_metal-bd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LUXS_THET8

Accession

Primary (citable) accession number: Q5SI29

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	December 21, 2004
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program