Q5SI02 (Q5SI02_THET8) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
1-pyrroline-5-carboxylate dehydrogenase EMBL BAD71401.1

Gene names

Ordered Locus Names:

TTHA1578

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	516 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the aldehyde dehydrogenase family. RuleBase RU003345

Ontologies

Keywords
Ligand	NAD PDB 2BHP PDB 2BJA PDB 2BJK PDB 2EHU PDB 2EII PDB 2EIT PDB 2J40 PDB 2J5N NADP PDB 2EHQ Nucleotide-binding PDB 2BHP PDB 2BJA PDB 2BJK PDB 2EHU PDB 2EII PDB 2EIT PDB 2J40 PDB 2J5N PDB 2EHQ
Molecular function	Oxidoreductase RuleBase RU003344
Technical term	3D-structure PDB 2BHP PDB 2BJA PDB 2BJK PDB 2EHU PDB 2EII PDB 2EIT PDB 2J40 PDB 2J5N PDB 2EHQ PDB 2EJD PDB 2EJL PDB 2IY6 PDB 2BHQ PDB 2EJ6 Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	181 – 183	3	NAD PDB 2BHP PDB 2BJA PDB 2BJK PDB 2EHU PDB 2EII PDB 2EIT PDB 2J40 PDB 2J5N
	Nucleotide binding	181 – 183	3	NADP PDB 2EHQ
	Nucleotide binding	207 – 210	4	NAD PDB 2BHP PDB 2BJA PDB 2BJK PDB 2EHU PDB 2EII PDB 2EIT PDB 2J40 PDB 2J5N
	Nucleotide binding	207 – 210	4	NADP PDB 2EHQ
	Nucleotide binding	240 – 241	2	NAD PDB 2BHP PDB 2BJA PDB 2BJK PDB 2EHU PDB 2EII PDB 2EIT PDB 2J40 PDB 2J5N
	Nucleotide binding	260 – 261	2	NAD PDB 2BHP PDB 2BJA PDB 2BJK PDB 2EHU PDB 2EII PDB 2EIT PDB 2J40 PDB 2J5N
	Nucleotide binding	260 – 261	2	NADP PDB 2EHQ
	Nucleotide binding	288 – 290	3	NAD PDB 2BHP PDB 2BJK PDB 2EHU PDB 2EII PDB 2EIT PDB 2J40 PDB 2J5N
	Nucleotide binding	288 – 289	2	NADP PDB 2EHQ
	Nucleotide binding	417 – 419	3	NAD PDB 2BHP PDB 2BJK PDB 2EHU PDB 2EII PDB 2EIT PDB 2J40 PDB 2J5N
	Nucleotide binding	417 – 419	3	NADP PDB 2EHQ
Sites
	Binding site	240	1	NADP PDB 2EHQ
	Binding site	322	1	NAD PDB 2BHP PDB 2BJK PDB 2EII PDB 2EIT
	Binding site	322	1	NADP PDB 2EHQ
Amino acid modifications
	Modified residue	322	1	Cysteine sulfenic acid (-SOH) PDB 2BJA PDB 2J40 PDB 2EJD PDB 2EJL

Sequences

	Sequence		Length	Mass (Da)
	Q5SI02 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 3546187E26C16F43	FASTA	516	57,046
	10 20 30 40 50 60 MTVEPFRNEP IETFQTEEAR RAMREALRRV REEFGRHYPL YIGGEWVDTK ERMVSLNPSA 70 80 90 100 110 120 PSEVVGTTAK AGKAEAEAAL EAAWKAFKTW KDWPQEDRSR LLLKAAALMR RRKRELEATL 130 140 150 160 170 180 VYEVGKNWVE ASADVAEAID FIEYYARAAL RYRYPAVEVV PYPGEDNESF YVPLGAGVVI 190 200 210 220 230 240 APWNFPVAIF TGMIVGPVAV GNTVIAKPAE DAVVVGAKVF EIFHEAGFPP GVVNFLPGVG 250 260 270 280 290 300 EEVGAYLVEH PRIRFINFTG SLEVGLKIYE AAGRLAPGQT WFKRAYVETG GKDAIIVDET 310 320 330 340 350 360 ADFDLAAEGV VVSAYGFQGQ KCSAASRLIL TQGAYEPVLE RVLKRAERLS VGPAEENPDL 370 380 390 400 410 420 GPVVSAEQER KVLSYIEIGK NEGQLVLGGK RLEGEGYFIA PTVFTEVPPK ARIAQEEIFG 430 440 450 460 470 480 PVLSVIRVKD FAEALEVAND TPYGLTGGVY SRKREHLEWA RREFHVGNLY FNRKITGALV 490 500 510 GVQPFGGFKL SGTNAKTGAL DYLRLFLEMK AVAERF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.
[2]	"Crystal structure analysis of delta1-pyrroline-5-carboxylate dehydrogenase in ternary complex with inhibitor and NAD." Inagaki E., Ohshima N., Sakamoto K., Yokoyama S., Tahirov T.H. Submitted (MAR-2007) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS), CYSTEINE SULFENIC ACID (-SOH) AT CYS-322.
[3]	"Crystal structure of Thermus thermophilus Delta1-pyrroline-5-carboxylate dehydrogenase." Inagaki E., Ohshima N., Takahashi H., Kuroishi C., Yokoyama S., Tahirov T.H. J. Mol. Biol. 362:490-501(2006) [PubMed: 16934832] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) IN COMPLEX WITH NAD, CYSTEINE SULFENIC ACID (-SOH) AT CYS-322.
[4]	"New insights into the binding mode of coenzymes: structure of Thermus thermophilus Delta1-pyrroline-5-carboxylate dehydrogenase complexed with NADP+." Inagaki E., Ohshima N., Sakamoto K., Babayeva N.D., Kato H., Yokoyama S., Tahirov T.H. Acta Crystallogr. F 63:462-465(2007) [PubMed: 17554163] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) IN COMPLEX WITH NADP.
[5]	"Crystal Structure of Ternary Complex of Delta1-Pyrroline-5-Carboxylate Dehydrogenase with Substrate Mimic and Co-Factoer." Inagaki E., Sakamoto K., Nishio M., Yokoyama S., Tahirov T.H. Submitted (SEP-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.63 ANGSTROMS) IN COMPLEX WITH NAD.
[6]	"Crystal Structure Analysis of delta1-pyrroline-5-carboxylate dehydrogenase in ternary complex with inhibitor and NAD." Inagaki E., Ohshima N. Submitted (MAR-2007) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH NAD.
[7]	"Crystal structure analysis of delta1-pyrroline-5-carboxylate dehydrogenase in ternary complex with inhibitor and NAD." Inagaki E., Ohshima N., Sakamoto K., Kato H., Yokoyama S., Tahirov T.H. Submitted (MAR-2007) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.88 ANGSTROMS) IN COMPLEX WITH NAD.
[8]	"Crystal Structure of Ternary Complex of Delta1-Pyrroline-5-Carboxylate Dehydrogenase with Substrate Mimic and Co-Factoer." Inagaki E., Sakamoto K., Nishio M., Yokoyama S., Tahirov T.H. Submitted (AUG-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH NAD, CYSTEINE SULFENIC ACID (-SOH) AT CYS-322.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AP008226 Genomic DNA. Translation: BAD71401.1.

RefSeq

YP_144844.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BHP	X-ray	1.80	A/B	1-516	[»]
2BHQ	X-ray	1.40	A/B	1-516	[»]
2BJA	X-ray	1.90	A/B	1-516	[»]
2BJK	X-ray	1.40	A/B	1-516	[»]
2EHQ	X-ray	1.55	A/B	1-516	[»]
2EHU	X-ray	1.80	A/B	1-516	[»]
2EII	X-ray	1.88	A/B	1-516	[»]
2EIT	X-ray	1.65	A/B	1-516	[»]
2EJ6	X-ray	2.06	A/B	1-516	[»]
2EJD	X-ray	1.85	A/B	1-516	[»]
2EJL	X-ray	1.50	A/B	1-516	[»]
2IY6	X-ray	1.80	A/B	1-516	[»]
2J40	X-ray	2.10	A/B	1-516	[»]
2J5N	X-ray	1.63	A/B	1-516	[»]

ProteinModelPortal

Q5SI02.

SMR

Q5SI02. Positions 1-516.

ModBase

Search...

Protein-protein interaction databases

STRING

Q5SI02.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

3167997.

GenomeReviews

Gene locus TTHA1578 in contig AP008226_GR.

KEGG

ttj:TTHA1578.

NMPDR

fig|300852.3.peg.1890.

PATRIC

23958113. VBITheThe93045_1549.

Phylogenomic databases

eggNOG

COG1012.

HOGENOM

HBG752218.

OMA

KITGALV.

PhylomeDB

Q5SI02.

ProtClustDB

PRK03137.

Enzyme and pathway databases

BioCyc

TTHE300852:TTHA1578-MONOMER.

Family and domain databases

InterPro

IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR005932. Delta1-pyrroline-5-COlate_DH-2.
[Graphical view]

Gene3D

G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit.
G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.

KO

K00294.

Pfam

PF00171. Aldedh. 1 hit.
[Graphical view]

SUPFAM

SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.

TIGRFAMs

TIGR01237. D1pyr5carbox2. 1 hit.

PROSITE

PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q5SI02_THET8

Accession

Primary (citable) accession number: Q5SI02

Entry history

Integrated into UniProtKB/TrEMBL:	December 21, 2004
Last sequence update:	December 21, 2004
Last modified:	December 14, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)