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Q5SHZ3 (DDL_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase
EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:TTHA1587
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus ›

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 319319D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341188

Regions

Domain120 – 315196ATP-grasp
Nucleotide binding147 – 19852ATP By similarity

Sites

Metal binding2701Magnesium or manganese 1 By similarity
Metal binding2821Magnesium or manganese 1 By similarity
Metal binding2821Magnesium or manganese 2 By similarity
Metal binding2841Magnesium or manganese 2 By similarity

Secondary structure

1 ........................................................ 319
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q5SHZ3 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: 85E6F0AA367A13AF

FASTA31934,666
        10         20         30         40         50         60 
MRVLLIAGGV SPEHEVSLLS AEGVLRHIPF PTDLAVIAQD GRWLLGEKAL TALEAKAAPE 

        70         80         90        100        110        120 
GEHPFPPPLS WERYDVVFPL LHGRFGEDGT VQGFLELLGK PYVGAGVAAS ALCMDKDLSK 

       130        140        150        160        170        180 
RVLAQAGVPV VPWVAVRKGE PPVVPFDPPF FVKPANTGSS VGISRVERFQ DLEAALALAF 

       190        200        210        220        230        240 
RYDEKAVVEK ALSPVRELEV GVLGNVFGEA SPVGEVRYEA PFYDYETKYT PGRAELLIPA 

       250        260        270        280        290        300 
PLDPGTQETV QELALKAYKV LGVRGMARVD FFLAEGELYL NELNTIPGFT PTSMYPRLFE 

       310 
AGGVAYPELL RRLVELALT

« Hide

References

[1]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP008226 Genomic DNA. Translation: BAD71410.1.
RefSeqYP_144853.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2FB9X-ray1.90A1-319[»]
2YZGX-ray2.30A/B/C1-319[»]
2YZMX-ray2.21A/B/C1-319[»]
2YZNX-ray2.60A/B/C1-319[»]
2ZDGX-ray2.20A/B/C/D1-319[»]
2ZDHX-ray1.90A/B/C/D1-319[»]
2ZDQX-ray2.30A/B1-319[»]
ProteinModelPortalQ5SHZ3.
SMRQ5SHZ3. Positions 1-319.
ModBaseSearch...

Protein-protein interaction databases

STRING300852.TTHA1587.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD71410; BAD71410; BAD71410.
GeneID3168507.
KEGGttj:TTHA1587.
PATRIC23958129. VBITheThe93045_1557.

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAALENWGS.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-1626-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ5SHZ3.

Entry information

Entry nameDDL_THET8
AccessionPrimary (citable) accession number: Q5SHZ3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: December 21, 2004
Last modified: May 29, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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