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Q5SHZ1 (RL25_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
50S ribosomal protein L25

Short name=TL5
Gene names
Name:rplY
Synonyms:rpl25, rptL5
Ordered Locus Names:TTHA1589
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length206 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This is one of 3 proteins that mediate the attachment of the 5S rRNA onto the large ribosomal subunit. HAMAP-Rule MF_01334

Subunit structure

Part of the 50S ribosomal subunit. Contacts the 5S rRNA; has been isolated in a complex with 5S rRNA and RL5, 5S rRNA and DNA binding protein II; 5S rRNA and RL18; 5S rRNA, RL5 and DNA binding protein II (Ref.4).

Sequence similarities

Belongs to the ribosomal protein L25P family. CTC subfamily.

Mass spectrometry

Molecular mass is 23207 Da from positions 1 - 206. Determined by MALDI. Ref.5

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 20620650S ribosomal protein L25 HAMAP-Rule MF_01334
PRO_0000181608

Experimental info

Sequence conflict43 – 453EFD → QRF AA sequence Ref.2
Sequence conflict60 – 7011ELPDGQSLPTL → QLRRGTRLSPE AA sequence Ref.2

Secondary structure

......................................................... 206
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q5SHZ1 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: ACC20EC1279181CD

FASTA20623,205
        10         20         30         40         50         60 
MEYRLKAYYR EGEKPSALRR AGKLPGVMYN RHLNRKVYVD LVEFDKVFRQ ASIHHVIVLE 

        70         80         90        100        110        120 
LPDGQSLPTL VRQVNLDKRR RRPEHVDFFV LSDEPVEMYV PLRFVGTPAG VRAGGVLQEI 

       130        140        150        160        170        180 
HRDILVKVSP RNIPEFIEVD VSGLEIGDSL HASDLKLPPG VELAVSPEET IAAVVPPEDV 

       190        200 
EKLAEEAAAE VAEPEVIKKG KEEEEE 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
[2]"The isolation and complete amino acid sequence of the ribosomal protein L36 from Thermus thermophilus and its zinc-binding motif."
Boysen R.I., Lorenz S., Kim J.S., Schroeder W.F.K.J., Erdmann V.A.
Endocyt. Cell Res. 11:41-58(1995)
Cited for: PROTEIN SEQUENCE OF 1-70.
[3]"Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus."
Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T.
Biol. Chem. 381:1079-1087(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-30.
[4]"Identification, purification and partial sequence of four Thermus thermophilus 5S rRNA binding proteins."
Kim J.-S., Boysen R.I., Schroeder W., Erdmann V.A., Gessner R.V.
Endocyt. Cell Res. 11:177-194(1996)
Cited for: PROTEIN SEQUENCE OF 1-22, ISOLATION OF 5S RRNA-ASSOCIATED COMPLEXES.
[5]"Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry."
Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A.
Proteomics 5:4818-4831(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY.
[6]"The path of messenger RNA through the ribosome."
Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F.
Cell 106:233-241(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
[7]"Crystal structure of the ribosome at 5.5 A resolution."
Yusupov M.M., Yusupova G.Z., Baucom A., Lieberman K., Earnest T.N., Cate J.H.D., Noller H.F.
Science 292:883-896(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008226 Genomic DNA. Translation: BAD71412.1.
RefSeqYP_144855.1. NC_006461.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GIYX-ray5.50V1-206[»]
2HGJX-ray5.00Y1-206[»]
2HGQX-ray5.50Y1-206[»]
2HGUX-ray4.51Y1-206[»]
2J01X-ray2.80Z1-206[»]
2J03X-ray2.80Z1-206[»]
2V47X-ray3.80Z1-206[»]
2V49X-ray3.80Z1-206[»]
2WDIX-ray3.30Z1-206[»]
2WDJX-ray3.30Z1-206[»]
2WDLX-ray3.50Z1-206[»]
2WDNX-ray3.50Z1-206[»]
2WH2X-ray3.45Z1-206[»]
2WH4X-ray3.45Z1-206[»]
2WRJX-ray3.60Z1-206[»]
2WRLX-ray3.60Z1-206[»]
2WROX-ray3.60Z1-206[»]
2WRRX-ray3.60Z1-206[»]
2X9SX-ray3.10Z1-206[»]
2X9UX-ray3.10Z1-206[»]
2XG0X-ray3.20Z1-206[»]
2XG2X-ray3.20Z1-206[»]
2XQEX-ray3.10Z1-206[»]
2XTGelectron microscopy7.80Z1-206[»]
2XUXelectron microscopy7.60Z1-206[»]
2Y0VX-ray3.10Z1-206[»]
2Y0XX-ray3.10Z1-206[»]
2Y0ZX-ray3.10Z1-206[»]
2Y11X-ray3.10Z1-206[»]
2Y13X-ray3.10Z1-206[»]
2Y15X-ray3.10Z1-206[»]
2Y17X-ray3.10Z1-206[»]
2Y19X-ray3.10Z1-206[»]
3FINelectron microscopy6.40Z3-179[»]
3HUXX-ray3.10Z1-206[»]
3HUZX-ray3.10Z1-206[»]
3I8FX-ray3.10V1-206[»]
3I8IX-ray3.10V1-206[»]
3I9CX-ray3.50V1-206[»]
3I9EX-ray3.50V1-206[»]
3KIRX-ray3.30Z1-206[»]
3KITX-ray3.30Z1-206[»]
3KIWX-ray3.60Z1-206[»]
3KIYX-ray3.60Z1-206[»]
3KNIX-ray3.00Z1-206[»]
3KNKX-ray3.00Z1-206[»]
3KNMX-ray3.45Z1-206[»]
3KNOX-ray3.45Z1-206[»]
3TVEX-ray3.10V1-175[»]
3TVHX-ray3.10V1-179[»]
3UXQX-ray3.20Z1-206[»]
3UXRX-ray3.20Z1-206[»]
3UYEX-ray3.00V1-206[»]
3UYGX-ray3.00V1-206[»]
3UZ1X-ray3.30V1-206[»]
3UZ2X-ray3.30V1-206[»]
3UZ8X-ray3.00V1-206[»]
3UZ9X-ray3.00V1-206[»]
3UZFX-ray3.30V1-206[»]
3UZHX-ray3.30V1-206[»]
3UZKX-ray3.30V1-206[»]
3UZNX-ray3.30V1-206[»]
3V23X-ray3.00Z1-206[»]
3V25X-ray3.00Z1-206[»]
3V27X-ray3.10Z1-206[»]
3V29X-ray3.10Z1-206[»]
3V2DX-ray2.70Z1-206[»]
3V2FX-ray2.70Z1-206[»]
3V6WX-ray3.90Z1-206[»]
3V6XX-ray3.90Z1-206[»]
3ZN9X-ray3.10Z1-206[»]
3ZNEX-ray3.10Z1-206[»]
3ZVPX-ray3.80Z1-206[»]
4ABSX-ray3.10Z1-206[»]
4B8GX-ray3.70Z1-206[»]
4B8IX-ray3.70Z1-206[»]
4BTDX-ray2.95Z2-206[»]
4BYCX-ray3.35Z1-206[»]
4BYEX-ray3.35Z1-206[»]
4DHAX-ray3.20Z1-206[»]
4DHCX-ray3.20Z1-206[»]
4EJBX-ray3.52Z1-206[»]
4EJCX-ray3.52Z1-206[»]
4G5LX-ray3.30V1-206[»]
4G5NX-ray3.30V1-206[»]
4G5UX-ray3.10V1-206[»]
4G5WX-ray3.10V1-206[»]
4JUXX-ray2.86Z1-206[»]
4K0MX-ray3.30Z3-178[»]
4K0QX-ray3.30Z3-178[»]
4NVVX-ray3.00Z1-206[»]
4NVXX-ray3.00Z1-206[»]
4NVZX-ray3.10Z1-206[»]
4NW1X-ray3.10Z1-206[»]
ProteinModelPortalQ5SHZ1.
SMRQ5SHZ1. Positions 1-185.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING300852.TTHA1589.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD71412; BAD71412; BAD71412.
GeneID3169859.
KEGGttj:TTHA1589.
PATRIC23958133. VBITheThe93045_1559.

Phylogenomic databases

eggNOGCOG1825.
HOGENOMHOG000214906.
KOK02897.
OMACIGVKLG.
OrthoDBEOG6SZ1PC.
PhylomeDBQ5SHZ1.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-1628-MONOMER.

Family and domain databases

Gene3D2.170.120.20. 1 hit.
2.40.240.10. 1 hit.
HAMAPMF_01334. Ribosomal_L25_CTC.
InterProIPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR020057. Ribosomal_L25_b-dom.
IPR001021. Ribosomal_L25_long.
IPR020055. Ribosomal_L25_short.
[Graphical view]
PfamPF01386. Ribosomal_L25p. 1 hit.
PF14693. Ribosomal_TL5_C. 1 hit.
[Graphical view]
ProDomPD012503. Ribosomal_L25. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00731. ctc_TL5. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ5SHZ1.

Entry information

Entry nameRL25_THET8
AccessionPrimary (citable) accession number: Q5SHZ1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: December 21, 2004
Last modified: July 9, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references