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Q5SHW1 (TRMB_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:TTHA1619
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus ›

Protein attributes

Sequence length311 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from electronic annotation. Source: GOC

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 311311tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000229205

Sites

Binding site281S-adenosyl-L-methionine By similarity
Binding site531S-adenosyl-L-methionine By similarity
Binding site1031S-adenosyl-L-methionine By similarity
Binding site1071Substrate By similarity
Binding site1391Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
Q5SHW1 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: 46161AC2A5DA32D8

FASTA31135,375
        10         20         30         40         50         60 
MLVVPARLHR WPPEVQDLFG REGPLVLEIG FGDGRFTAEL ARSRPDWLVL GAEVSAASVL 

        70         80         90        100        110        120 
RALRRMRREG LANVRLYHGQ GPFALRNLVL PGTLDQVIVN FPDPWPKKRH QERRLLREAF 

       130        140        150        160        170        180 
FRRLSTRLKP GGSLLLTTDH EEYFRFALEE AERTGLYRVE VRPPPEAHLR TKYALKWKEA 

       190        200        210        220        230        240 
GRAFFHAAFI KLREDPAPWP PLRRYDVAHA LLSGELPQDL ALEKTPVRLK EGVAVFLEVA 

       250        260        270        280        290        300 
RGKEGFYVLT HVEEEDLTQD LLLEVRKSAR GVYAGVSRFG SPLITEAVKG AVRALVDRLE 

       310 
AHGLEVVQDH T

« Hide

References

[1]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP008226 Genomic DNA. Translation: BAD71442.1.
RefSeqYP_144885.1. NC_006461.1.

3D structure databases

ProteinModelPortalQ5SHW1.
ModBaseSearch...

Protein-protein interaction databases

STRING300852.TTHA1619.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD71442; BAD71442; BAD71442.
GeneID3168435.
KEGGttj:TTHA1619.
PATRIC23958193. VBITheThe93045_1589.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000099573.
KOK03439.
OMATKYALKW.
ProtClustDBCLSK445190.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-1658-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMB_THET8
AccessionPrimary (citable) accession number: Q5SHW1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: December 21, 2004
Last modified: May 29, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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