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Q5SHW1 (TRMB_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:TTHA1619
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length311 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 311311tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000229205

Sites

Active site1031 By similarity
Binding site281S-adenosyl-L-methionine By similarity
Binding site531S-adenosyl-L-methionine By similarity
Binding site1031S-adenosyl-L-methionine By similarity
Binding site1071Substrate By similarity
Binding site1391Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5SHW1 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: 46161AC2A5DA32D8

FASTA31135,375
        10         20         30         40         50         60 
MLVVPARLHR WPPEVQDLFG REGPLVLEIG FGDGRFTAEL ARSRPDWLVL GAEVSAASVL 

        70         80         90        100        110        120 
RALRRMRREG LANVRLYHGQ GPFALRNLVL PGTLDQVIVN FPDPWPKKRH QERRLLREAF 

       130        140        150        160        170        180 
FRRLSTRLKP GGSLLLTTDH EEYFRFALEE AERTGLYRVE VRPPPEAHLR TKYALKWKEA 

       190        200        210        220        230        240 
GRAFFHAAFI KLREDPAPWP PLRRYDVAHA LLSGELPQDL ALEKTPVRLK EGVAVFLEVA 

       250        260        270        280        290        300 
RGKEGFYVLT HVEEEDLTQD LLLEVRKSAR GVYAGVSRFG SPLITEAVKG AVRALVDRLE 

       310 
AHGLEVVQDH T 

« Hide

References

[1]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008226 Genomic DNA. Translation: BAD71442.1.
RefSeqYP_144885.1. NC_006461.1.

3D structure databases

ProteinModelPortalQ5SHW1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING300852.TTHA1619.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD71442; BAD71442; BAD71442.
GeneID3168435.
KEGGttj:TTHA1619.
PATRIC23958193. VBITheThe93045_1589.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000099573.
KOK03439.
OMATKYALKW.
OrthoDBEOG6K6VBC.
ProtClustDBCLSK445190.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-1658-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_THET8
AccessionPrimary (citable) accession number: Q5SHW1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: December 21, 2004
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways