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Q5SHQ3 (RL6_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
50S ribosomal protein L6
Gene names
Name:rplF
Ordered Locus Names:TTHA1677
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length180 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center. HAMAP-Rule MF_01365

Subunit structure

Part of the 50S ribosomal subunit.

Sequence similarities

Belongs to the ribosomal protein L6P family.

Mass spectrometry

Molecular mass is 19401 Da from positions 2 - 180. Determined by MALDI. Ref.4

Ontologies

Keywords
   LigandRNA-binding
rRNA-binding
   Molecular functionRibonucleoprotein
Ribosomal protein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentribosome

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionrRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

structural constituent of ribosome

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 18017950S ribosomal protein L6 HAMAP-Rule MF_01365
PRO_0000131072

Experimental info

Sequence conflict161S → Q Ref.2
Sequence conflict181E → Q Ref.2
Sequence conflict19 – 202VA → AV AA sequence Ref.3
Sequence conflict201A → S Ref.2
Sequence conflict231R → L Ref.2
Sequence conflict251K → L AA sequence Ref.3
Sequence conflict271K → Y AA sequence Ref.3
Sequence conflict341E → S in CAA39899. Ref.2
Sequence conflict41 – 422MR → LK in CAA39899. Ref.2
Sequence conflict481G → N in CAA39899. Ref.2
Sequence conflict621K → R in CAA39899. Ref.2
Sequence conflict841S → V in CAA39899. Ref.2
Sequence conflict102 – 1032AL → SI in CAA39899. Ref.2
Sequence conflict1311V → I in CAA39899. Ref.2

Secondary structure

...................................... 180
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q5SHQ3 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 1913833C0C30E95D

FASTA18019,532
        10         20         30         40         50         60 
MSRIGRLPIP VPKGVSVEVA PGRVKVKGPK GELEVPVSPE MRVVVEEGVV RVERPSDERR 

        70         80         90        100        110        120 
HKSLHGLTRT LIANAVKGVS EGYSKELLIK GIGYRARLVG RALELTVGFS HPVVVEPPEG 

       130        140        150        160        170        180 
ITFEVPEPTR VRVSGIDKQK VGQVAANIRA IRKPSAYHEK GIYYAGEPVR LKPGKAGAKK 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
[2]"Analysis of the spc ribosomal protein operon of Thermus aquaticus."
Jahn O., Hartmann R.K., Erdmann V.A.
Eur. J. Biochem. 197:733-740(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-131.
[3]"Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus."
Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T.
Biol. Chem. 381:1079-1087(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-29.
[4]"Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry."
Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A.
Proteomics 5:4818-4831(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY.
[5]"The path of messenger RNA through the ribosome."
Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F.
Cell 106:233-241(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
[6]"Crystal structure of the ribosome at 5.5 A resolution."
Yusupov M.M., Yusupova G.Z., Baucom A., Lieberman K., Earnest T.N., Cate J.H.D., Noller H.F.
Science 292:883-896(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008226 Genomic DNA. Translation: BAD71500.1.
X56552 Genomic DNA. Translation: CAA39899.1.
PIRS15442.
RefSeqYP_144943.1. NC_006461.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GIYX-ray5.50H26-177[»]
1YL3X-ray5.50H24-177[»]
2HGJX-ray5.00H1-180[»]
2HGQX-ray5.50H1-180[»]
2HGUX-ray4.51H1-180[»]
2J01X-ray2.80H2-180[»]
2J03X-ray2.80H2-180[»]
2V47X-ray3.50H2-180[»]
2V49X-ray3.50H2-180[»]
2WDIX-ray3.30H1-180[»]
2WDJX-ray3.30H1-180[»]
2WDLX-ray3.50H1-180[»]
2WDNX-ray3.50H1-180[»]
2WH2X-ray3.45H1-180[»]
2WH4X-ray3.45H1-180[»]
2WRJX-ray3.60H1-180[»]
2WRLX-ray3.60H1-180[»]
2WROX-ray3.60H1-180[»]
2WRRX-ray3.60H1-180[»]
2X9SX-ray3.10H1-180[»]
2X9UX-ray3.10H1-180[»]
2XG0X-ray3.20H1-180[»]
2XG2X-ray3.20H1-180[»]
2XQEX-ray3.10H1-180[»]
2XTGelectron microscopy7.80H1-180[»]
2XUXelectron microscopy7.60H1-180[»]
2Y0VX-ray3.10H1-180[»]
2Y0XX-ray3.10H1-180[»]
2Y0ZX-ray3.10H1-180[»]
2Y11X-ray3.10H1-180[»]
2Y13X-ray3.10H1-180[»]
2Y15X-ray3.10H1-180[»]
2Y17X-ray3.10H1-180[»]
2Y19X-ray3.10H1-180[»]
3FINelectron microscopy6.40H12-171[»]
3HUXX-ray3.10H1-180[»]
3HUZX-ray3.10H1-180[»]
3I8FX-ray3.10H1-180[»]
3I8IX-ray3.10H1-180[»]
3I9CX-ray3.50H1-180[»]
3I9EX-ray3.50H1-180[»]
3KIRX-ray3.30H1-180[»]
3KITX-ray3.30H1-180[»]
3KIWX-ray3.60H1-180[»]
3KIYX-ray3.60H1-180[»]
3KNIX-ray3.00H1-180[»]
3KNKX-ray3.00H1-180[»]
3KNMX-ray3.45H1-180[»]
3KNOX-ray3.45H1-180[»]
3TVEX-ray3.10H2-171[»]
3TVHX-ray3.10H2-171[»]
3UXQX-ray3.20H1-180[»]
3UXRX-ray3.20H1-180[»]
3UYEX-ray3.00H1-180[»]
3UYGX-ray3.00H1-180[»]
3UZ1X-ray3.30H1-180[»]
3UZ2X-ray3.30H1-180[»]
3UZ8X-ray3.00H1-180[»]
3UZ9X-ray3.00H1-180[»]
3UZFX-ray3.30H1-180[»]
3UZHX-ray3.30H1-180[»]
3UZKX-ray3.30H1-180[»]
3UZNX-ray3.30H1-180[»]
3V23X-ray3.00H1-180[»]
3V25X-ray3.00H1-180[»]
3V27X-ray3.10H1-180[»]
3V29X-ray3.10H1-180[»]
3V2DX-ray2.70H1-180[»]
3V2FX-ray2.70H1-180[»]
3V6WX-ray3.90H1-180[»]
3V6XX-ray3.90H1-180[»]
3ZN9X-ray3.10H1-180[»]
3ZNEX-ray3.10H1-180[»]
3ZVPX-ray3.80H1-180[»]
4ABSX-ray3.10H1-180[»]
4B8GX-ray3.70H1-180[»]
4B8IX-ray3.70H1-180[»]
4BTDX-ray2.95H1-180[»]
4BYCX-ray3.35H1-180[»]
4BYEX-ray3.35H1-180[»]
4DHAX-ray3.20H1-180[»]
4DHCX-ray3.20H1-180[»]
4EJBX-ray3.52H1-180[»]
4EJCX-ray3.52H1-180[»]
4G5LX-ray3.30H1-180[»]
4G5NX-ray3.30H1-180[»]
4G5UX-ray3.10H1-180[»]
4G5WX-ray3.10H1-180[»]
4JUXX-ray2.86H1-180[»]
4K0MX-ray3.30H12-170[»]
4K0QX-ray3.30H12-170[»]
4NVVX-ray3.00H1-180[»]
4NVXX-ray3.00H1-180[»]
4NVZX-ray3.10H1-180[»]
4NW1X-ray3.10H1-180[»]
ProteinModelPortalQ5SHQ3.
SMRQ5SHQ3. Positions 4-177.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING300852.TTHA1677.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD71500; BAD71500; BAD71500.
GeneID3169807.
KEGGttj:TTHA1677.
PATRIC23958309. VBITheThe93045_1647.

Phylogenomic databases

eggNOGCOG0097.
HOGENOMHOG000039903.
KOK02933.
OMAAANKNAR.
OrthoDBEOG67DPRD.
PhylomeDBQ5SHQ3.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-1716-MONOMER.

Family and domain databases

Gene3D3.90.930.12. 2 hits.
HAMAPMF_01365_B. Ribosomal_L6_B.
InterProIPR000702. Ribosomal_L6.
IPR020040. Ribosomal_L6_a/b-dom.
IPR019906. Ribosomal_L6_bac-type.
[Graphical view]
PANTHERPTHR11655. PTHR11655. 1 hit.
PfamPF00347. Ribosomal_L6. 2 hits.
[Graphical view]
PIRSFPIRSF002162. Ribosomal_L6. 1 hit.
PRINTSPR00059. RIBOSOMALL6.
SUPFAMSSF56053. SSF56053. 2 hits.
TIGRFAMsTIGR03654. L6_bact. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ5SHQ3.

Entry information

Entry nameRL6_THET8
AccessionPrimary (citable) accession number: Q5SHQ3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 85 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references