Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

50S ribosomal protein L6

Gene

rplF

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L6UniRule annotation
Gene namesi
Name:rplFUniRule annotation
Ordered Locus Names:TTHA1677
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001310722 – 18050S ribosomal protein L6Add BLAST179

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit.

Protein-protein interaction databases

STRINGi300852.TTHA1677.

Structurei

Secondary structure

1180
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi21 – 28Combined sources8
Beta strandi31 – 36Combined sources6
Beta strandi47 – 49Combined sources3
Helixi59 – 79Combined sources21
Beta strandi86 – 93Combined sources8
Beta strandi95 – 99Combined sources5
Beta strandi102 – 106Combined sources5
Beta strandi108 – 111Combined sources4
Beta strandi113 – 115Combined sources3
Beta strandi121 – 124Combined sources4
Beta strandi126 – 138Combined sources13
Helixi139 – 150Combined sources12
Beta strandi161 – 163Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VVJX-ray3.44RH/YH1-180[»]
1VY4X-ray2.60BH/DH1-180[»]
1VY5X-ray2.55BH/DH1-180[»]
1VY6X-ray2.90BH/DH1-180[»]
1VY7X-ray2.80BH/DH1-180[»]
4L47X-ray3.22RH/YH1-180[»]
4L71X-ray3.90RH/YH1-180[»]
4LELX-ray3.90RH/YH1-180[»]
4LFZX-ray3.92RH/YH1-180[»]
4LNTX-ray2.94RH/YH1-180[»]
4LSKX-ray3.48RH/YH1-180[»]
4LT8X-ray3.14RH/YH1-180[»]
4P6FX-ray3.60RH/YH1-180[»]
4P70X-ray3.68RH/YH1-180[»]
4TUAX-ray3.60RH/YH1-180[»]
4TUBX-ray3.60RH/YH1-180[»]
4TUCX-ray3.60RH/YH1-180[»]
4TUDX-ray3.60RH/YH1-180[»]
4TUEX-ray3.50RH/YH1-180[»]
4V42X-ray5.50BH26-177[»]
4V4PX-ray5.50H24-177[»]
4V4XX-ray5.00BH1-180[»]
4V4YX-ray5.50BH1-180[»]
4V4ZX-ray4.51BH1-180[»]
4V51X-ray2.80BH/DH2-180[»]
4V5AX-ray3.50BH/DH2-180[»]
4V5CX-ray3.30BH/DH1-180[»]
4V5DX-ray3.50BH/DH1-180[»]
4V5EX-ray3.45BH/DH1-180[»]
4V5FX-ray3.60BH/DH1-180[»]
4V5GX-ray3.60BH/DH1-180[»]
4V5JX-ray3.10BH/DH1-180[»]
4V5KX-ray3.20BH/DH1-180[»]
4V5LX-ray3.10BH1-180[»]
4V5Melectron microscopy7.80BH1-180[»]
4V5Nelectron microscopy7.60BH1-180[»]
4V5PX-ray3.10BH/DH1-180[»]
4V5QX-ray3.10BH/DH1-180[»]
4V5RX-ray3.10BH/DH1-180[»]
4V5SX-ray3.10BH/DH1-180[»]
4V68electron microscopy6.40BH12-171[»]
4V6AX-ray3.10BH/DH1-180[»]
4V6FX-ray3.10AH/DH1-180[»]
4V6GX-ray3.50BH/DH1-180[»]
4V7JX-ray3.30AH/BH1-180[»]
4V7KX-ray3.60AH/BH1-180[»]
4V7LX-ray3.00BH/DH1-180[»]
4V7MX-ray3.45BH/DH1-180[»]
4V7WX-ray3.00BH/DH1-180[»]
4V7XX-ray3.00BH/DH1-180[»]
4V7YX-ray3.00BH/DH1-180[»]
4V7ZX-ray3.10BH/DH1-180[»]
4V87X-ray3.10AH/DH2-171[»]
4V8AX-ray3.20AH/BH1-180[»]
4V8BX-ray3.00BH/DH1-180[»]
4V8CX-ray3.30AH/BH1-180[»]
4V8DX-ray3.00BH/DH1-180[»]
4V8EX-ray3.30AH/CH1-180[»]
4V8FX-ray3.30AH/DH1-180[»]
4V8GX-ray3.00BH/DH1-180[»]
4V8HX-ray3.10BH/DH1-180[»]
4V8IX-ray2.70BH/DH1-180[»]
4V8JX-ray3.90BH/DH1-180[»]
4V8NX-ray3.10BH/DH1-180[»]
4V8OX-ray3.80BH1-180[»]
4V8QX-ray3.10AH1-180[»]
4V8UX-ray3.70BH/DH1-180[»]
4V8XX-ray3.35BH/DH1-180[»]
4V90X-ray2.95BH1-180[»]
4V95X-ray3.20BH/DH1-180[»]
4V97X-ray3.52BH/DH1-180[»]
4V9AX-ray3.30BH/DH1-180[»]
4V9BX-ray3.10BH/DH1-180[»]
4V9HX-ray2.86BH1-180[»]
4V9IX-ray3.30BH/DH12-170[»]
4V9RX-ray3.00BH/DH1-180[»]
4V9SX-ray3.10BH/DH1-180[»]
4W2EX-ray2.90H1-180[»]
4W2FX-ray2.40BH/DH1-180[»]
4W2GX-ray2.55BH/DH1-180[»]
4W2HX-ray2.70BH/DH1-180[»]
4W2IX-ray2.70BH/DH1-180[»]
4W4GX-ray3.30RH/YH1-180[»]
4WPOX-ray2.80AH/CH1-180[»]
4WQ1X-ray3.1051/591-180[»]
4WQFX-ray2.80AH/CH1-180[»]
4WQRX-ray3.1551/591-180[»]
4WQUX-ray2.80AH/CH1-180[»]
4WQYX-ray2.80AH/CH1-180[»]
4WR6X-ray3.0551/591-180[»]
4WRAX-ray3.0551/591-180[»]
4WROX-ray3.05511-180[»]
4WSDX-ray2.9551/591-180[»]
4WSMX-ray3.3051/591-180[»]
4WT1X-ray3.0551/591-180[»]
4WT8X-ray3.40CF/DF12-170[»]
4WU1X-ray3.2051/591-180[»]
4WZDX-ray3.1051/591-180[»]
4WZOX-ray3.3051/591-180[»]
4Y4OX-ray2.301H/2H1-180[»]
4Y4PX-ray2.501H/2H1-180[»]
4YPBX-ray3.40RH/YH1-180[»]
4YZVX-ray3.10RH/YH1-180[»]
4Z3SX-ray2.651H/2H1-180[»]
4Z8CX-ray2.901H/2H1-180[»]
4ZERX-ray3.101H/2H2-175[»]
4ZSNX-ray3.60RH/YH1-180[»]
5A9Zelectron microscopy4.70AH4-177[»]
5AA0electron microscopy5.00AH4-177[»]
5CZPX-ray3.30RH/YH1-180[»]
5D8BX-ray3.63AB/E1-180[»]
5DFEX-ray3.10RH/YH1-180[»]
5DOXX-ray3.101H/2H1-180[»]
5DOYX-ray2.601H/2H1-180[»]
5E7KX-ray3.2051/591-180[»]
5E81X-ray2.9551/591-180[»]
5EL4X-ray3.1551/591-180[»]
5EL5X-ray3.1551/591-180[»]
5EL6X-ray3.1051/591-180[»]
5EL7X-ray3.1551/591-180[»]
5F8KX-ray2.801H/2H2-175[»]
5FDUX-ray2.901H/2H2-175[»]
5FDVX-ray2.801H/2H2-175[»]
5HAUX-ray3.001H/2H1-180[»]
5HCPX-ray2.891H/2H1-180[»]
5HCQX-ray2.801H/2H1-180[»]
5HCRX-ray2.801H/2H1-180[»]
5HD1X-ray2.701H/2H1-180[»]
5IB7X-ray2.9951/591-180[»]
5IB8X-ray3.1351/591-180[»]
5IBBX-ray2.9651/591-180[»]
5IMQelectron microscopy3.80e1-180[»]
5IMRelectron microscopy-e1-180[»]
5J30X-ray3.20RH/YH1-180[»]
5J3CX-ray3.04RH/YH1-180[»]
5J4BX-ray2.601H/2H1-180[»]
5J4CX-ray2.801H/2H1-180[»]
5J8BX-ray2.60H1-180[»]
ProteinModelPortaliQ5SHQ3.
SMRiQ5SHQ3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SHQ3.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L6P family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108R5J. Bacteria.
COG0097. LUCA.
HOGENOMiHOG000039903.
KOiK02933.
OMAiLIANMVE.
PhylomeDBiQ5SHQ3.

Family and domain databases

Gene3Di3.90.930.12. 2 hits.
HAMAPiMF_01365_B. Ribosomal_L6_B. 1 hit.
InterProiIPR000702. Ribosomal_L6.
IPR020040. Ribosomal_L6_a/b-dom.
IPR019906. Ribosomal_L6_bac-type.
[Graphical view]
PANTHERiPTHR11655. PTHR11655. 1 hit.
PfamiPF00347. Ribosomal_L6. 2 hits.
[Graphical view]
PIRSFiPIRSF002162. Ribosomal_L6. 1 hit.
PRINTSiPR00059. RIBOSOMALL6.
SUPFAMiSSF56053. SSF56053. 2 hits.
TIGRFAMsiTIGR03654. L6_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5SHQ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRIGRLPIP VPKGVSVEVA PGRVKVKGPK GELEVPVSPE MRVVVEEGVV
60 70 80 90 100
RVERPSDERR HKSLHGLTRT LIANAVKGVS EGYSKELLIK GIGYRARLVG
110 120 130 140 150
RALELTVGFS HPVVVEPPEG ITFEVPEPTR VRVSGIDKQK VGQVAANIRA
160 170 180
IRKPSAYHEK GIYYAGEPVR LKPGKAGAKK
Length:180
Mass (Da):19,532
Last modified:January 23, 2007 - v3
Checksum:i1913833C0C30E95D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16S → Q (PubMed:2029902).Curated1
Sequence conflicti18E → Q (PubMed:2029902).Curated1
Sequence conflicti19 – 20VA → AV AA sequence (PubMed:11154066).Curated2
Sequence conflicti20A → S (PubMed:2029902).Curated1
Sequence conflicti23R → L (PubMed:2029902).Curated1
Sequence conflicti25K → L AA sequence (PubMed:11154066).Curated1
Sequence conflicti27K → Y AA sequence (PubMed:11154066).Curated1
Sequence conflicti34E → S in CAA39899 (PubMed:2029902).Curated1
Sequence conflicti41 – 42MR → LK in CAA39899 (PubMed:2029902).Curated2
Sequence conflicti48G → N in CAA39899 (PubMed:2029902).Curated1
Sequence conflicti62K → R in CAA39899 (PubMed:2029902).Curated1
Sequence conflicti84S → V in CAA39899 (PubMed:2029902).Curated1
Sequence conflicti102 – 103AL → SI in CAA39899 (PubMed:2029902).Curated2
Sequence conflicti131V → I in CAA39899 (PubMed:2029902).Curated1

Mass spectrometryi

Molecular mass is 19401 Da from positions 2 - 180. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71500.1.
X56552 Genomic DNA. Translation: CAA39899.1.
PIRiS15442.
RefSeqiWP_011173704.1. NC_006461.1.
YP_144943.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71500; BAD71500; BAD71500.
GeneIDi3169807.
KEGGittj:TTHA1677.
PATRICi23958309. VBITheThe93045_1647.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71500.1.
X56552 Genomic DNA. Translation: CAA39899.1.
PIRiS15442.
RefSeqiWP_011173704.1. NC_006461.1.
YP_144943.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VVJX-ray3.44RH/YH1-180[»]
1VY4X-ray2.60BH/DH1-180[»]
1VY5X-ray2.55BH/DH1-180[»]
1VY6X-ray2.90BH/DH1-180[»]
1VY7X-ray2.80BH/DH1-180[»]
4L47X-ray3.22RH/YH1-180[»]
4L71X-ray3.90RH/YH1-180[»]
4LELX-ray3.90RH/YH1-180[»]
4LFZX-ray3.92RH/YH1-180[»]
4LNTX-ray2.94RH/YH1-180[»]
4LSKX-ray3.48RH/YH1-180[»]
4LT8X-ray3.14RH/YH1-180[»]
4P6FX-ray3.60RH/YH1-180[»]
4P70X-ray3.68RH/YH1-180[»]
4TUAX-ray3.60RH/YH1-180[»]
4TUBX-ray3.60RH/YH1-180[»]
4TUCX-ray3.60RH/YH1-180[»]
4TUDX-ray3.60RH/YH1-180[»]
4TUEX-ray3.50RH/YH1-180[»]
4V42X-ray5.50BH26-177[»]
4V4PX-ray5.50H24-177[»]
4V4XX-ray5.00BH1-180[»]
4V4YX-ray5.50BH1-180[»]
4V4ZX-ray4.51BH1-180[»]
4V51X-ray2.80BH/DH2-180[»]
4V5AX-ray3.50BH/DH2-180[»]
4V5CX-ray3.30BH/DH1-180[»]
4V5DX-ray3.50BH/DH1-180[»]
4V5EX-ray3.45BH/DH1-180[»]
4V5FX-ray3.60BH/DH1-180[»]
4V5GX-ray3.60BH/DH1-180[»]
4V5JX-ray3.10BH/DH1-180[»]
4V5KX-ray3.20BH/DH1-180[»]
4V5LX-ray3.10BH1-180[»]
4V5Melectron microscopy7.80BH1-180[»]
4V5Nelectron microscopy7.60BH1-180[»]
4V5PX-ray3.10BH/DH1-180[»]
4V5QX-ray3.10BH/DH1-180[»]
4V5RX-ray3.10BH/DH1-180[»]
4V5SX-ray3.10BH/DH1-180[»]
4V68electron microscopy6.40BH12-171[»]
4V6AX-ray3.10BH/DH1-180[»]
4V6FX-ray3.10AH/DH1-180[»]
4V6GX-ray3.50BH/DH1-180[»]
4V7JX-ray3.30AH/BH1-180[»]
4V7KX-ray3.60AH/BH1-180[»]
4V7LX-ray3.00BH/DH1-180[»]
4V7MX-ray3.45BH/DH1-180[»]
4V7WX-ray3.00BH/DH1-180[»]
4V7XX-ray3.00BH/DH1-180[»]
4V7YX-ray3.00BH/DH1-180[»]
4V7ZX-ray3.10BH/DH1-180[»]
4V87X-ray3.10AH/DH2-171[»]
4V8AX-ray3.20AH/BH1-180[»]
4V8BX-ray3.00BH/DH1-180[»]
4V8CX-ray3.30AH/BH1-180[»]
4V8DX-ray3.00BH/DH1-180[»]
4V8EX-ray3.30AH/CH1-180[»]
4V8FX-ray3.30AH/DH1-180[»]
4V8GX-ray3.00BH/DH1-180[»]
4V8HX-ray3.10BH/DH1-180[»]
4V8IX-ray2.70BH/DH1-180[»]
4V8JX-ray3.90BH/DH1-180[»]
4V8NX-ray3.10BH/DH1-180[»]
4V8OX-ray3.80BH1-180[»]
4V8QX-ray3.10AH1-180[»]
4V8UX-ray3.70BH/DH1-180[»]
4V8XX-ray3.35BH/DH1-180[»]
4V90X-ray2.95BH1-180[»]
4V95X-ray3.20BH/DH1-180[»]
4V97X-ray3.52BH/DH1-180[»]
4V9AX-ray3.30BH/DH1-180[»]
4V9BX-ray3.10BH/DH1-180[»]
4V9HX-ray2.86BH1-180[»]
4V9IX-ray3.30BH/DH12-170[»]
4V9RX-ray3.00BH/DH1-180[»]
4V9SX-ray3.10BH/DH1-180[»]
4W2EX-ray2.90H1-180[»]
4W2FX-ray2.40BH/DH1-180[»]
4W2GX-ray2.55BH/DH1-180[»]
4W2HX-ray2.70BH/DH1-180[»]
4W2IX-ray2.70BH/DH1-180[»]
4W4GX-ray3.30RH/YH1-180[»]
4WPOX-ray2.80AH/CH1-180[»]
4WQ1X-ray3.1051/591-180[»]
4WQFX-ray2.80AH/CH1-180[»]
4WQRX-ray3.1551/591-180[»]
4WQUX-ray2.80AH/CH1-180[»]
4WQYX-ray2.80AH/CH1-180[»]
4WR6X-ray3.0551/591-180[»]
4WRAX-ray3.0551/591-180[»]
4WROX-ray3.05511-180[»]
4WSDX-ray2.9551/591-180[»]
4WSMX-ray3.3051/591-180[»]
4WT1X-ray3.0551/591-180[»]
4WT8X-ray3.40CF/DF12-170[»]
4WU1X-ray3.2051/591-180[»]
4WZDX-ray3.1051/591-180[»]
4WZOX-ray3.3051/591-180[»]
4Y4OX-ray2.301H/2H1-180[»]
4Y4PX-ray2.501H/2H1-180[»]
4YPBX-ray3.40RH/YH1-180[»]
4YZVX-ray3.10RH/YH1-180[»]
4Z3SX-ray2.651H/2H1-180[»]
4Z8CX-ray2.901H/2H1-180[»]
4ZERX-ray3.101H/2H2-175[»]
4ZSNX-ray3.60RH/YH1-180[»]
5A9Zelectron microscopy4.70AH4-177[»]
5AA0electron microscopy5.00AH4-177[»]
5CZPX-ray3.30RH/YH1-180[»]
5D8BX-ray3.63AB/E1-180[»]
5DFEX-ray3.10RH/YH1-180[»]
5DOXX-ray3.101H/2H1-180[»]
5DOYX-ray2.601H/2H1-180[»]
5E7KX-ray3.2051/591-180[»]
5E81X-ray2.9551/591-180[»]
5EL4X-ray3.1551/591-180[»]
5EL5X-ray3.1551/591-180[»]
5EL6X-ray3.1051/591-180[»]
5EL7X-ray3.1551/591-180[»]
5F8KX-ray2.801H/2H2-175[»]
5FDUX-ray2.901H/2H2-175[»]
5FDVX-ray2.801H/2H2-175[»]
5HAUX-ray3.001H/2H1-180[»]
5HCPX-ray2.891H/2H1-180[»]
5HCQX-ray2.801H/2H1-180[»]
5HCRX-ray2.801H/2H1-180[»]
5HD1X-ray2.701H/2H1-180[»]
5IB7X-ray2.9951/591-180[»]
5IB8X-ray3.1351/591-180[»]
5IBBX-ray2.9651/591-180[»]
5IMQelectron microscopy3.80e1-180[»]
5IMRelectron microscopy-e1-180[»]
5J30X-ray3.20RH/YH1-180[»]
5J3CX-ray3.04RH/YH1-180[»]
5J4BX-ray2.601H/2H1-180[»]
5J4CX-ray2.801H/2H1-180[»]
5J8BX-ray2.60H1-180[»]
ProteinModelPortaliQ5SHQ3.
SMRiQ5SHQ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1677.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71500; BAD71500; BAD71500.
GeneIDi3169807.
KEGGittj:TTHA1677.
PATRICi23958309. VBITheThe93045_1647.

Phylogenomic databases

eggNOGiENOG4108R5J. Bacteria.
COG0097. LUCA.
HOGENOMiHOG000039903.
KOiK02933.
OMAiLIANMVE.
PhylomeDBiQ5SHQ3.

Miscellaneous databases

EvolutionaryTraceiQ5SHQ3.

Family and domain databases

Gene3Di3.90.930.12. 2 hits.
HAMAPiMF_01365_B. Ribosomal_L6_B. 1 hit.
InterProiIPR000702. Ribosomal_L6.
IPR020040. Ribosomal_L6_a/b-dom.
IPR019906. Ribosomal_L6_bac-type.
[Graphical view]
PANTHERiPTHR11655. PTHR11655. 1 hit.
PfamiPF00347. Ribosomal_L6. 2 hits.
[Graphical view]
PIRSFiPIRSF002162. Ribosomal_L6. 1 hit.
PRINTSiPR00059. RIBOSOMALL6.
SUPFAMiSSF56053. SSF56053. 2 hits.
TIGRFAMsiTIGR03654. L6_bact. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRL6_THET8
AccessioniPrimary (citable) accession number: Q5SHQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.