Q5SHQ1 (RS14Z_THET8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
30S ribosomal protein S14 type Z

Gene names

Name:	rpsZ
Synonyms:	rpsN
Ordered Locus Names:	TTHA1679

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus

Protein attributes

Sequence length	61 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site By similarity. Binds 16S rRNA in center of the 30S subunit head. HAMAP-Rule MF_01364_B
Cofactor	Binds 1 zinc ion per subunit. Ref.5
Subunit structure	Part of the 30S ribosomal subunit. Forms a tight complex with proteins S3 and S10, which cover it. Ref.7 Ref.8 Ref.9 Ref.10 Ref.12 Ref.13 Ref.15 Ref.16
Sequence similarities	Belongs to the ribosomal protein S14P family. Zinc-binding S14P subfamily.
Mass spectrometry	Molecular mass is 7010 Da from positions 2 - 61. Determined by MALDI. Ref.6

Ontologies

Keywords
Ligand	Metal-binding RNA-binding Zinc rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Cellular_component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.2 Ref.4

Chain

2 – 61

60

30S ribosomal protein S14 type Z HAMAP-Rule MF_01364_B

PRO_0000130955

Sites

Metal binding

24

1

Zinc

Metal binding

27

1

Zinc

Metal binding

40

1

Zinc

Metal binding

43

1

Zinc

Experimental info

Sequence conflict

36

1

F → Y in CAA39897. Ref.1

Sequence conflict

57

1

R → K in CAA39897. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

61

Helix Strand Turn

Details...

Helix

4 – 9

6

Beta strand

10 – 12

3

Helix

17 – 19

3

Turn

25 – 27

3

Beta strand

31 – 33

3

Turn

35 – 37

3

Beta strand

38 – 40

3

Helix

41 – 50

10

Beta strand

53 – 55

3

Beta strand

56 – 58

3

Sequences

	Sequence		Length	Mass (Da)
	Q5SHQ1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: BEE8AB1E1E86B531	FASTA	61	7,140
	10 20 30 40 50 60 MARKALIEKA KRTPKFKVRA YTRCVRCGRA RSVYRFFGLC RICLRELAHK GQLPGVRKAS W

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the spc ribosomal protein operon of Thermus aquaticus." Jahn O., Hartmann R.K., Erdmann V.A. Eur. J. Biochem. 197:733-740(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Studies on S14 protein from Thermus thermophilus possessing zinc finger-like motifs." Tsiboli P., Choli T. Biol. Chem. Hoppe-Seyler 376:127-130(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-61.
[3]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.
[4]	"Purification and characterization of the 30S ribosomal proteins from the bacterium Thermus thermophilus." Tsiboli P., Herfurth E., Choli T. Eur. J. Biochem. 226:169-177(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-23.
[5]	"Studies on the Zn-containing S14 ribosomal protein from Thermus thermophilus." Tsiboli P., Triantafillidou D., Franceschi F., Choli-Papadopoulou T. Eur. J. Biochem. 256:136-141(1998) [PubMed] [Europe PMC] [Abstract] Cited for: COFACTOR.
[6]	"Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry." Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A. Proteomics 5:4818-4831(2005) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY.
[7]	"Structure of the 30S ribosomal subunit." Wimberly B.T., Brodersen D.E., Clemons W.M. Jr., Morgan-Warren R.J., Carter A.P., Vonrhein C., Hartsch T., Ramakrishnan V. Nature 407:327-339(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.05 ANGSTROMS) OF THE 30S SUBUNIT.
[8]	"Structure of functionally activated small ribosomal subunit at 3.3 A resolution." Schluenzen F., Tocilj A., Zarivach R., Harms J., Gluehmann M., Janell D., Bashan A., Bartels H., Agmon I., Franceschi F., Yonath A. Cell 102:615-623(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) OF THE 30S SUBUNIT.
[9]	"The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit." Brodersen D.E., Clemons W.M. Jr., Carter A.P., Morgan-Warren R.J., Wimberly B.T., Ramakrishnan V. Cell 103:1143-1154(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) OF THE 30S SUBUNIT.
[10]	"Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics." Carter A.P., Clemons W.M. Jr., Brodersen D.E., Morgan-Warren R.J., Wimberly B.T., Ramakrishnan V. Nature 407:340-348(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 30S SUBUNIT.
[11]	"The path of messenger RNA through the ribosome." Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F. Cell 106:233-241(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
[12]	"Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3." Pioletti M., Schluenzen F., Harms J., Zarivach R., Gluehmann M., Avila H., Bashan A., Bartels H., Auerbach T., Jacobi C., Hartsch T., Yonath A., Franceschi F. EMBO J. 20:1829-1839(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF THE 30S SUBUNIT.
[13]	"Crystal structure of an initiation factor bound to the 30S ribosomal subunit." Carter A.P., Clemons W.M. Jr., Brodersen D.E., Morgan-Warren R.J., Hartsch T., Wimberly B.T., Ramakrishnan V. Science 291:498-501(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF THE 30S SUBUNIT.
[14]	"Crystal structure of the ribosome at 5.5 A resolution." Yusupov M.M., Yusupova G.Z., Baucom A., Lieberman K., Earnest T.N., Cate J.H.D., Noller H.F. Science 292:883-896(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME.
[15]	"Recognition of cognate transfer RNA by the 30S ribosomal subunit." Ogle J.M., Brodersen D.E., Clemons W.M. Jr., Tarry M.J., Carter A.P., Ramakrishnan V. Science 292:897-902(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.11 ANGSTROMS) OF THE 30S SUBUNIT.
[16]	"Crystal structure of the 30S ribosomal subunit from Thermus thermophilus: structure of the proteins and their interactions with 16S RNA." Brodersen D.E., Clemons W.M. Jr., Carter A.P., Wimberly B.T., Ramakrishnan V. J. Mol. Biol. 316:725-768(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.05 ANGSTROMS) OF THE 30S SUBUNIT.
+	Additional computationally mapped references.

Web resources

T.thermophilus ribosome structure and function

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X56552 Genomic DNA. Translation: CAA39897.1.
S78490 Genomic DNA. Translation: AAB34721.1.
AP008226 Genomic DNA. Translation: BAD71502.1.

RefSeq

YP_144945.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FJG	X-ray	3.00	N	1-61	[»]
1GIX	X-ray	5.50	Q	1-61	[»]
1HNW	X-ray	3.40	N	1-61	[»]
1HNX	X-ray	3.40	N	1-61	[»]
1HNZ	X-ray	3.30	N	1-61	[»]
1HR0	X-ray	3.20	N	1-61	[»]
1I94	X-ray	3.20	N	2-61	[»]
1I95	X-ray	4.50	N	2-61	[»]
1I96	X-ray	4.20	N	2-61	[»]
1I97	X-ray	4.50	N	2-61	[»]
1IBK	X-ray	3.31	N	1-61	[»]
1IBL	X-ray	3.11	N	1-61	[»]
1IBM	X-ray	3.31	N	1-61	[»]
1J5E	X-ray	3.05	N	2-61	[»]
1JGO	X-ray	5.60	Q	1-61	[»]
1JGP	X-ray	7.00	Q	1-61	[»]
1JGQ	X-ray	5.00	Q	1-61	[»]
1L1U	model	-	N	1-61	[»]
1N32	X-ray	3.00	N	2-61	[»]
1N33	X-ray	3.35	N	2-61	[»]
1N34	X-ray	3.80	N	2-61	[»]
1N36	X-ray	3.65	N	2-61	[»]
1XMO	X-ray	3.25	N	1-61	[»]
1XMQ	X-ray	3.00	N	1-61	[»]
1XNQ	X-ray	3.05	N	1-61	[»]
1XNR	X-ray	3.10	N	1-61	[»]
1YL4	X-ray	5.50	Q	1-61	[»]
2B64	X-ray	5.90	N	1-61	[»]
2B9M	X-ray	6.76	N	1-61	[»]
2B9O	X-ray	6.46	N	1-61	[»]
2E5L	X-ray	3.30	N	2-61	[»]
2F4V	X-ray	3.80	N	1-61	[»]
2HGI	X-ray	5.00	Q	1-61	[»]
2HGP	X-ray	5.50	Q	1-61	[»]
2HGR	X-ray	4.51	Q	1-61	[»]
2HHH	X-ray	3.35	N	1-61	[»]
2J00	X-ray	2.80	N	1-61	[»]
2J02	X-ray	2.80	N	1-61	[»]
2OW8	X-ray	3.71	o	-	[»]
2UU9	X-ray	3.10	N	1-61	[»]
2UUA	X-ray	2.90	N	1-61	[»]
2UUB	X-ray	2.90	N	1-61	[»]
2UUC	X-ray	3.10	N	1-61	[»]
2UXB	X-ray	3.10	N	1-61	[»]
2UXC	X-ray	2.90	N	1-61	[»]
2UXD	X-ray	3.20	N	1-61	[»]
2V46	X-ray	3.80	N	1-61	[»]
2V48	X-ray	3.80	N	1-61	[»]
2VQE	X-ray	2.50	N	1-61	[»]
2VQF	X-ray	2.90	N	1-61	[»]
2WDG	X-ray	3.30	N	1-61	[»]
2WDH	X-ray	3.30	N	1-61	[»]
2WDK	X-ray	3.50	N	1-61	[»]
2WDM	X-ray	3.50	N	1-61	[»]
2WH1	X-ray	3.45	N	1-61	[»]
2WH3	X-ray	3.45	N	1-61	[»]
2WRI	X-ray	3.60	N	1-61	[»]
2WRK	X-ray	3.60	N	1-61	[»]
2WRN	X-ray	3.60	N	1-61	[»]
2WRQ	X-ray	3.60	N	1-61	[»]
2X9R	X-ray	3.10	N	1-61	[»]
2X9T	X-ray	3.10	N	1-61	[»]
2XFZ	X-ray	3.20	N	1-61	[»]
2XG1	X-ray	3.20	N	1-61	[»]
2XQD	X-ray	3.10	N	1-61	[»]
2XSY	electron microscopy	7.80	N	1-61	[»]
2XUY	electron microscopy	7.60	N	1-61	[»]
2Y0U	X-ray	3.10	N	1-61	[»]
2Y0W	X-ray	3.10	N	1-61	[»]
2Y0Y	X-ray	3.10	N	1-61	[»]
2Y10	X-ray	3.10	N	1-61	[»]
2Y12	X-ray	3.10	N	1-61	[»]
2Y14	X-ray	3.10	N	1-61	[»]
2Y16	X-ray	3.10	N	1-61	[»]
2Y18	X-ray	3.10	N	1-61	[»]
2ZM6	X-ray	3.30	N	2-61	[»]
3FIC	electron microscopy	6.40	N	2-61	[»]
3HUW	X-ray	3.10	N	1-61	[»]
3HUY	X-ray	3.10	N	1-61	[»]
3I8G	X-ray	3.10	Q	1-61	[»]
3I8H	X-ray	3.10	Q	1-61	[»]
3I9B	X-ray	3.50	Q	1-61	[»]
3I9D	X-ray	3.50	Q	1-61	[»]
3KIQ	X-ray	3.30	n	1-61	[»]
3KIS	X-ray	3.30	n	1-61	[»]
3KIU	X-ray	3.60	n	1-61	[»]
3KIX	X-ray	3.60	n	1-61	[»]
3KNH	X-ray	3.00	N	1-61	[»]
3KNJ	X-ray	3.15	N	1-61	[»]
3KNL	X-ray	3.45	N	1-61	[»]
3KNN	X-ray	3.45	N	1-61	[»]
3OGE	X-ray	3.00	N	1-61	[»]
3OGY	X-ray	3.00	N	1-61	[»]
3OHC	X-ray	3.00	N	1-61	[»]
3OHD	X-ray	3.00	N	1-61	[»]
3OHY	X-ray	3.00	N	1-61	[»]
3OI0	X-ray	3.00	N	1-61	[»]
3OI2	X-ray	3.10	N	1-61	[»]
3OI4	X-ray	3.10	N	1-61	[»]
3OTO	X-ray	3.69	N	1-61	[»]
3T1H	X-ray	3.11	N	1-61	[»]
3T1Y	X-ray	2.80	N	1-61	[»]
3TVF	X-ray	3.10	Q	1-61	[»]
3TVG	X-ray	3.10	Q	1-61	[»]
3UXS	X-ray	3.20	N	1-61	[»]
3UXT	X-ray	3.20	N	1-61	[»]
3UYD	X-ray	3.00	Q	1-61	[»]
3UYF	X-ray	3.00	Q	1-61	[»]
3UZ3	X-ray	3.30	Q	1-61	[»]
3UZ4	X-ray	3.30	Q	1-61	[»]
3UZ6	X-ray	3.00	Q	1-61	[»]
3UZ7	X-ray	3.00	Q	1-61	[»]
3UZG	X-ray	3.30	Q	1-61	[»]
3UZI	X-ray	3.30	Q	1-61	[»]
3UZL	X-ray	3.30	Q	1-61	[»]
3UZM	X-ray	3.30	Q	1-61	[»]
3V22	X-ray	3.00	N	1-61	[»]
3V24	X-ray	3.00	N	1-61	[»]
3V26	X-ray	3.10	N	1-61	[»]
3V28	X-ray	3.10	N	1-61	[»]
3V2C	X-ray	2.70	N	1-61	[»]
3V2E	X-ray	2.70	N	1-61	[»]
3ZVO	X-ray	3.80	N	1-61	[»]
4ABR	X-ray	3.10	N	1-61	[»]
4AQY	X-ray	3.50	N	2-61	[»]
4DH9	X-ray	3.20	N	1-61	[»]
4DHB	X-ray	3.20	N	1-61	[»]
4DR1	X-ray	3.60	N	1-61	[»]
4DR2	X-ray	3.25	N	1-61	[»]
4DR3	X-ray	3.35	N	1-61	[»]
4DR4	X-ray	3.97	N	1-61	[»]
4DR5	X-ray	3.45	N	1-61	[»]
4DR6	X-ray	3.30	N	1-61	[»]
4DR7	X-ray	3.75	N	1-61	[»]
4DUY	X-ray	3.39	N	1-61	[»]
4DUZ	X-ray	3.65	N	1-61	[»]
4DV0	X-ray	3.85	N	1-61	[»]
4DV1	X-ray	3.85	N	1-61	[»]
4DV2	X-ray	3.65	N	1-61	[»]
4DV3	X-ray	3.55	N	1-61	[»]
4DV4	X-ray	3.65	N	1-61	[»]
4DV5	X-ray	3.68	N	1-61	[»]
4DV6	X-ray	3.30	N	1-61	[»]
4DV7	X-ray	3.29	N	1-61	[»]
4G5K	X-ray	3.30	Q	1-61	[»]
4G5M	X-ray	3.30	Q	1-61	[»]
4G5T	X-ray	3.10	Q	1-61	[»]
4G5V	X-ray	3.10	Q	1-61	[»]

ProteinModelPortal

Q5SHQ1.

SMR

Q5SHQ1. Positions 2-61.

ModBase

Search...

Protein-protein interaction databases

STRING

300852.TTHA1679.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAD71502; BAD71502; BAD71502.

GeneID

3169802.

KEGG

ttj:TTHA1679.

PATRIC

23958313. VBITheThe93045_1649.

Phylogenomic databases

eggNOG

COG0199.

HOGENOM

HOG000039865.

KO

K02954.

OMA

KMANEGL.

ProtClustDB

PRK08061.

Family and domain databases

HAMAP

MF_01364_B. Ribosomal_S14_2_B.

InterPro

IPR001209. Ribosomal_S14.
IPR018271. Ribosomal_S14_CS.
IPR023053. Ribosomal_S14_Z.
[Graphical view]

PANTHER

PTHR19836. PTHR19836. 1 hit.

Pfam

PF00253. Ribosomal_S14. 1 hit.
[Graphical view]

PROSITE

PS00527. RIBOSOMAL_S14. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q5SHQ1.

Entry information

Entry name

RS14Z_THET8

Accession

Primary (citable) accession number: Q5SHQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program