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Q5SHP8

- RL14_THET8

UniProt

Q5SHP8 - RL14_THET8

Protein

50S ribosomal protein L14

Gene

rplN

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    This protein binds directly to 23S ribosomal RNA.By similarity
    Contacts the 16S rRNA of the 30S subunit in two different positions helping to form bridges B5 and B8.

    GO - Molecular functioni

    1. rRNA binding Source: UniProtKB-HAMAP
    2. structural constituent of ribosome Source: InterPro

    GO - Biological processi

    1. translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Ligandi

    RNA-binding, rRNA-binding

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-1721-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    50S ribosomal protein L14UniRule annotation
    Gene namesi
    Name:rplNUniRule annotation
    Ordered Locus Names:TTHA1682
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    ProteomesiUP000000532: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. large ribosomal subunit Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12212250S ribosomal protein L14PRO_0000128566Add
    BLAST

    Interactioni

    Subunit structurei

    Part of the 50S ribosomal subunit. Contacts protein L19. Forms a bridge to the 30S subunit in the 70S ribosome, contacting the 16S rRNA.

    Protein-protein interaction databases

    STRINGi300852.TTHA1682.

    Structurei

    Secondary structure

    1
    122
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 104
    Beta strandi12 – 2615
    Turni27 – 293
    Beta strandi38 – 469
    Beta strandi48 – 525
    Beta strandi57 – 648
    Beta strandi69 – 713
    Beta strandi72 – 743
    Beta strandi76 – 816
    Beta strandi83 – 875
    Turni89 – 913
    Beta strandi93 – 964
    Beta strandi100 – 1034
    Helixi105 – 1084
    Helixi112 – 1176
    Beta strandi118 – 1225

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GIYX-ray5.50N57-121[»]
    1YL3X-ray5.50N52-121[»]
    2HGJX-ray5.00N1-122[»]
    2HGQX-ray5.50N1-122[»]
    2HGUX-ray4.51N1-122[»]
    2J01X-ray2.80O1-122[»]
    2J03X-ray2.80O1-122[»]
    2V47X-ray3.80O1-122[»]
    2V49X-ray3.80O1-122[»]
    2WDIX-ray3.30O1-122[»]
    2WDJX-ray3.30O1-122[»]
    2WDLX-ray3.50O1-122[»]
    2WDNX-ray3.50O1-122[»]
    2WH2X-ray3.45O1-122[»]
    2WH4X-ray3.45O1-122[»]
    2WRJX-ray3.60O1-122[»]
    2WRLX-ray3.60O1-122[»]
    2WROX-ray3.60O1-122[»]
    2WRRX-ray3.60O1-122[»]
    2X9SX-ray3.10O1-122[»]
    2X9UX-ray3.10O1-122[»]
    2XG0X-ray3.20O1-122[»]
    2XG2X-ray3.20O1-122[»]
    2XQEX-ray3.10O1-122[»]
    2XTGelectron microscopy7.80O1-122[»]
    2XUXelectron microscopy7.60O1-122[»]
    2Y0VX-ray3.10O1-122[»]
    2Y0XX-ray3.10O1-122[»]
    2Y0ZX-ray3.10O1-122[»]
    2Y11X-ray3.10O1-122[»]
    2Y13X-ray3.10O1-122[»]
    2Y15X-ray3.10O1-122[»]
    2Y17X-ray3.10O1-122[»]
    2Y19X-ray3.10O1-122[»]
    3FINelectron microscopy6.40O1-122[»]
    3HUXX-ray3.10O1-122[»]
    3HUZX-ray3.10O1-122[»]
    3I8FX-ray3.10N1-122[»]
    3I8IX-ray3.10N1-122[»]
    3I9CX-ray3.50N1-122[»]
    3I9EX-ray3.50N1-122[»]
    3KIRX-ray3.30O1-122[»]
    3KITX-ray3.30O1-122[»]
    3KIWX-ray3.60O1-122[»]
    3KIYX-ray3.60O1-122[»]
    3KNIX-ray3.00O1-122[»]
    3KNKX-ray3.00O1-122[»]
    3KNMX-ray3.45O1-122[»]
    3KNOX-ray3.45O1-122[»]
    3TVEX-ray3.10N1-122[»]
    3TVHX-ray3.10N1-122[»]
    3UXQX-ray3.20O1-122[»]
    3UXRX-ray3.20O1-122[»]
    3UYEX-ray3.00N1-122[»]
    3UYGX-ray3.00N1-122[»]
    3UZ1X-ray3.30N1-122[»]
    3UZ2X-ray3.30N1-122[»]
    3UZ8X-ray3.00N1-122[»]
    3UZ9X-ray3.00N1-122[»]
    3UZFX-ray3.30N1-122[»]
    3UZHX-ray3.30N1-122[»]
    3UZKX-ray3.30N1-122[»]
    3UZNX-ray3.30N1-122[»]
    3V23X-ray3.00O1-122[»]
    3V25X-ray3.00O1-122[»]
    3V27X-ray3.10O1-122[»]
    3V29X-ray3.10O1-122[»]
    3V2DX-ray2.70O1-122[»]
    3V2FX-ray2.70O1-122[»]
    3V6WX-ray3.90O1-122[»]
    3V6XX-ray3.90O1-122[»]
    3ZN9X-ray3.10O1-122[»]
    3ZNEX-ray3.10O1-122[»]
    3ZVPX-ray3.80O1-122[»]
    4ABSX-ray3.10O1-122[»]
    4B8GX-ray3.70O1-122[»]
    4B8IX-ray3.70O1-122[»]
    4BTDX-ray2.95O1-122[»]
    4BYCX-ray3.35O1-122[»]
    4BYEX-ray3.35O1-122[»]
    4DHAX-ray3.20O1-122[»]
    4DHCX-ray3.20O1-122[»]
    4EJBX-ray3.52O1-122[»]
    4EJCX-ray3.52O1-122[»]
    4G5LX-ray3.30N1-122[»]
    4G5NX-ray3.30N1-122[»]
    4G5UX-ray3.10N1-122[»]
    4G5WX-ray3.10N1-122[»]
    4JUXX-ray2.86O1-122[»]
    4K0MX-ray3.30O1-122[»]
    4K0QX-ray3.30O1-122[»]
    4NVVX-ray3.00O1-122[»]
    4NVXX-ray3.00O1-122[»]
    4NVZX-ray3.10O1-122[»]
    4NW1X-ray3.10O1-122[»]
    ProteinModelPortaliQ5SHP8.
    SMRiQ5SHP8. Positions 1-122.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ5SHP8.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L14P family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0093.
    HOGENOMiHOG000183702.
    KOiK02874.
    OMAiTPNMPVK.
    OrthoDBiEOG6GBMJ6.
    PhylomeDBiQ5SHP8.

    Family and domain databases

    Gene3Di2.40.150.20. 1 hit.
    HAMAPiMF_01367. Ribosomal_L14.
    InterProiIPR005745. Ribosomal_L14_bac-type.
    IPR019972. Ribosomal_L14_CS.
    IPR023571. Ribosomal_L14_dom.
    IPR000218. Ribosomal_L14b/L23e.
    [Graphical view]
    PANTHERiPTHR11761. PTHR11761. 1 hit.
    PfamiPF00238. Ribosomal_L14. 1 hit.
    [Graphical view]
    SUPFAMiSSF50193. SSF50193. 1 hit.
    TIGRFAMsiTIGR01067. rplN_bact. 1 hit.
    PROSITEiPS00049. RIBOSOMAL_L14. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5SHP8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIQPQTYLEV ADNTGARKIM CIRVLKGSNA KYATVGDVIV ASVKEAIPRG    50
    AVKEGDVVKA VVVRTKKEIK RPDGSAIRFD DNAAVIINNQ LEPRGTRVFG 100
    PVARELREKG FMKIVSLAPE VL 122
    Length:122
    Mass (Da):13,303
    Last modified:December 21, 2004 - v1
    Checksum:i9305B92EBAAB9B6C
    GO

    Mass spectrometryi

    Molecular mass is 13304 Da from positions 1 - 122. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008226 Genomic DNA. Translation: BAD71505.1.
    RefSeqiWP_011228844.1. NC_006461.1.
    YP_144948.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD71505; BAD71505; BAD71505.
    GeneIDi3168727.
    KEGGittj:TTHA1682.
    PATRICi23958319. VBITheThe93045_1652.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008226 Genomic DNA. Translation: BAD71505.1 .
    RefSeqi WP_011228844.1. NC_006461.1.
    YP_144948.1. NC_006461.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GIY X-ray 5.50 N 57-121 [» ]
    1YL3 X-ray 5.50 N 52-121 [» ]
    2HGJ X-ray 5.00 N 1-122 [» ]
    2HGQ X-ray 5.50 N 1-122 [» ]
    2HGU X-ray 4.51 N 1-122 [» ]
    2J01 X-ray 2.80 O 1-122 [» ]
    2J03 X-ray 2.80 O 1-122 [» ]
    2V47 X-ray 3.80 O 1-122 [» ]
    2V49 X-ray 3.80 O 1-122 [» ]
    2WDI X-ray 3.30 O 1-122 [» ]
    2WDJ X-ray 3.30 O 1-122 [» ]
    2WDL X-ray 3.50 O 1-122 [» ]
    2WDN X-ray 3.50 O 1-122 [» ]
    2WH2 X-ray 3.45 O 1-122 [» ]
    2WH4 X-ray 3.45 O 1-122 [» ]
    2WRJ X-ray 3.60 O 1-122 [» ]
    2WRL X-ray 3.60 O 1-122 [» ]
    2WRO X-ray 3.60 O 1-122 [» ]
    2WRR X-ray 3.60 O 1-122 [» ]
    2X9S X-ray 3.10 O 1-122 [» ]
    2X9U X-ray 3.10 O 1-122 [» ]
    2XG0 X-ray 3.20 O 1-122 [» ]
    2XG2 X-ray 3.20 O 1-122 [» ]
    2XQE X-ray 3.10 O 1-122 [» ]
    2XTG electron microscopy 7.80 O 1-122 [» ]
    2XUX electron microscopy 7.60 O 1-122 [» ]
    2Y0V X-ray 3.10 O 1-122 [» ]
    2Y0X X-ray 3.10 O 1-122 [» ]
    2Y0Z X-ray 3.10 O 1-122 [» ]
    2Y11 X-ray 3.10 O 1-122 [» ]
    2Y13 X-ray 3.10 O 1-122 [» ]
    2Y15 X-ray 3.10 O 1-122 [» ]
    2Y17 X-ray 3.10 O 1-122 [» ]
    2Y19 X-ray 3.10 O 1-122 [» ]
    3FIN electron microscopy 6.40 O 1-122 [» ]
    3HUX X-ray 3.10 O 1-122 [» ]
    3HUZ X-ray 3.10 O 1-122 [» ]
    3I8F X-ray 3.10 N 1-122 [» ]
    3I8I X-ray 3.10 N 1-122 [» ]
    3I9C X-ray 3.50 N 1-122 [» ]
    3I9E X-ray 3.50 N 1-122 [» ]
    3KIR X-ray 3.30 O 1-122 [» ]
    3KIT X-ray 3.30 O 1-122 [» ]
    3KIW X-ray 3.60 O 1-122 [» ]
    3KIY X-ray 3.60 O 1-122 [» ]
    3KNI X-ray 3.00 O 1-122 [» ]
    3KNK X-ray 3.00 O 1-122 [» ]
    3KNM X-ray 3.45 O 1-122 [» ]
    3KNO X-ray 3.45 O 1-122 [» ]
    3TVE X-ray 3.10 N 1-122 [» ]
    3TVH X-ray 3.10 N 1-122 [» ]
    3UXQ X-ray 3.20 O 1-122 [» ]
    3UXR X-ray 3.20 O 1-122 [» ]
    3UYE X-ray 3.00 N 1-122 [» ]
    3UYG X-ray 3.00 N 1-122 [» ]
    3UZ1 X-ray 3.30 N 1-122 [» ]
    3UZ2 X-ray 3.30 N 1-122 [» ]
    3UZ8 X-ray 3.00 N 1-122 [» ]
    3UZ9 X-ray 3.00 N 1-122 [» ]
    3UZF X-ray 3.30 N 1-122 [» ]
    3UZH X-ray 3.30 N 1-122 [» ]
    3UZK X-ray 3.30 N 1-122 [» ]
    3UZN X-ray 3.30 N 1-122 [» ]
    3V23 X-ray 3.00 O 1-122 [» ]
    3V25 X-ray 3.00 O 1-122 [» ]
    3V27 X-ray 3.10 O 1-122 [» ]
    3V29 X-ray 3.10 O 1-122 [» ]
    3V2D X-ray 2.70 O 1-122 [» ]
    3V2F X-ray 2.70 O 1-122 [» ]
    3V6W X-ray 3.90 O 1-122 [» ]
    3V6X X-ray 3.90 O 1-122 [» ]
    3ZN9 X-ray 3.10 O 1-122 [» ]
    3ZNE X-ray 3.10 O 1-122 [» ]
    3ZVP X-ray 3.80 O 1-122 [» ]
    4ABS X-ray 3.10 O 1-122 [» ]
    4B8G X-ray 3.70 O 1-122 [» ]
    4B8I X-ray 3.70 O 1-122 [» ]
    4BTD X-ray 2.95 O 1-122 [» ]
    4BYC X-ray 3.35 O 1-122 [» ]
    4BYE X-ray 3.35 O 1-122 [» ]
    4DHA X-ray 3.20 O 1-122 [» ]
    4DHC X-ray 3.20 O 1-122 [» ]
    4EJB X-ray 3.52 O 1-122 [» ]
    4EJC X-ray 3.52 O 1-122 [» ]
    4G5L X-ray 3.30 N 1-122 [» ]
    4G5N X-ray 3.30 N 1-122 [» ]
    4G5U X-ray 3.10 N 1-122 [» ]
    4G5W X-ray 3.10 N 1-122 [» ]
    4JUX X-ray 2.86 O 1-122 [» ]
    4K0M X-ray 3.30 O 1-122 [» ]
    4K0Q X-ray 3.30 O 1-122 [» ]
    4NVV X-ray 3.00 O 1-122 [» ]
    4NVX X-ray 3.00 O 1-122 [» ]
    4NVZ X-ray 3.10 O 1-122 [» ]
    4NW1 X-ray 3.10 O 1-122 [» ]
    ProteinModelPortali Q5SHP8.
    SMRi Q5SHP8. Positions 1-122.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 300852.TTHA1682.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD71505 ; BAD71505 ; BAD71505 .
    GeneIDi 3168727.
    KEGGi ttj:TTHA1682.
    PATRICi 23958319. VBITheThe93045_1652.

    Phylogenomic databases

    eggNOGi COG0093.
    HOGENOMi HOG000183702.
    KOi K02874.
    OMAi TPNMPVK.
    OrthoDBi EOG6GBMJ6.
    PhylomeDBi Q5SHP8.

    Enzyme and pathway databases

    BioCyci TTHE300852:GH8R-1721-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q5SHP8.

    Family and domain databases

    Gene3Di 2.40.150.20. 1 hit.
    HAMAPi MF_01367. Ribosomal_L14.
    InterProi IPR005745. Ribosomal_L14_bac-type.
    IPR019972. Ribosomal_L14_CS.
    IPR023571. Ribosomal_L14_dom.
    IPR000218. Ribosomal_L14b/L23e.
    [Graphical view ]
    PANTHERi PTHR11761. PTHR11761. 1 hit.
    Pfami PF00238. Ribosomal_L14. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50193. SSF50193. 1 hit.
    TIGRFAMsi TIGR01067. rplN_bact. 1 hit.
    PROSITEi PS00049. RIBOSOMAL_L14. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Thermus thermophilus HB8."
      Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB8 / ATCC 27634 / DSM 579.
    2. "Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus."
      Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T.
      Biol. Chem. 381:1079-1087(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-15.
    3. "Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry."
      Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A.
      Proteomics 5:4818-4831(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: MASS SPECTROMETRY.
    4. "The path of messenger RNA through the ribosome."
      Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F.
      Cell 106:233-241(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
    5. Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME, INTERSUBUNIT BRIDGE FORMATION.

    Entry informationi

    Entry nameiRL14_THET8
    AccessioniPrimary (citable) accession number: Q5SHP8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: December 21, 2004
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3