Reviewed, UniProtKB/Swiss-Prot Q5SHP6 (RL29_THET8)

Names and origin

Protein names

Recommended name:
    50S ribosomal protein L29

Gene names

Name:	rpmC
Ordered Locus Names:	TTHA1684

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Complete proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	72 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit. HAMAP MF_00374
Subunit structure	Part of the 50S ribosomal subunit. HAMAP MF_00374
Sequence similarities	Belongs to the ribosomal protein L29P family.
Mass spectrometry	Molecular mass is 8652 Da from positions 1 - 72. Determined by MALDI. Ref.4

Ontologies

Keywords
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 72

72

50S ribosomal protein L29 HAMAP MF_00374

PRO_0000130483

Experimental info

Sequence conflict

6

1

V → M AA sequence Ref.2

Sequence conflict

19

1

V → M AA sequence Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

72

Helix Strand Turn

Details...

Turn

14 – 16

3

Helix

17 – 31

15

Beta strand

32 – 35

4

Helix

45 – 47

3

Beta strand

50 – 54

5

Sequences

	Sequence		Length	Mass (Da)
	Q5SHP6-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: C113B72000929584	FASTA	72	8,650
	10 20 30 40 50 60 MKLSEVRKQL EEARKLSPVE LEKLVREKKR ELMELRFQAS IGQLSQNHKI RDLKRQIARL 70 LTVLNEKRRQ NA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Systematic, computer-assisted optimisation of the isolation of Thermus thermophilus 50S ribosomal proteins by reversed-phase high-performance liquid chromatography." Boysen R.I., Erdmann V.A., Hearn M.T. J. Biochem. Biophys. Methods 37:69-89(1998) [PubMed: 9825301] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-59.
[3]	"Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus." Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T. Biol. Chem. 381:1079-1087(2000) [PubMed: 11154066] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-20.
[4]	"Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry." Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A. Proteomics 5:4818-4831(2005) [PubMed: 16287167] [Abstract] Cited for: MASS SPECTROMETRY.
[5]	"The path of messenger RNA through the ribosome." Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F. Cell 106:233-241(2001) [PubMed: 11511350] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
[6]	"Crystal structure of the ribosome at 5.5 A resolution." Yusupov M.M., Yusupova G.Z., Baucom A., Lieberman K., Earnest T.N., Cate J.H.D., Noller H.F. Science 292:883-896(2001) [PubMed: 11283358] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AP008226 Genomic DNA. Translation: BAD71507.1.

RefSeq

YP_144950.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GIY	X-ray	5.50	W	1-72	[»]
1YL3	X-ray	5.50	W	1-72	[»]
2HGJ	X-ray	5.00	1	1-72	[»]
2HGQ	X-ray	5.50	1	1-72	[»]
2HGU	X-ray	4.51	1	1-72	[»]
2J01	X-ray	2.80	2	1-72	[»]
2J03	X-ray	2.80	2	1-72	[»]
2V47	X-ray	3.80	2	1-72	[»]
2V49	X-ray	3.80	2	1-72	[»]
2WDI	X-ray	3.30	2	1-72	[»]
2WDJ	X-ray	3.30	2	1-72	[»]
2WDL	X-ray	3.50	2	1-72	[»]
2WDN	X-ray	3.50	2	1-72	[»]
2WH2	X-ray	3.45	2	1-72	[»]
2WH4	X-ray	3.45	2	1-72	[»]
2WRJ	X-ray	3.60	2	1-72	[»]
2WRL	X-ray	3.60	2	1-72	[»]
2WRO	X-ray	3.60	2	1-72	[»]
2WRR	X-ray	3.60	2	1-72	[»]
3FIN	electron microscopy	6.40	2	12-62	[»]
3HUX	X-ray	3.10	2	1-72	[»]
3HUZ	X-ray	3.10	2	1-72	[»]
3KIR	X-ray	3.30	2	1-72	[»]
3KIT	X-ray	3.30	2	1-72	[»]
3KIW	X-ray	3.60	2	1-72	[»]
3KIY	X-ray	3.60	2	1-72	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q5SHP6.

Genome annotation databases

GeneID

3169832.

GenomeReviews

Gene locus TTHA1684 in contig AP008226_GR.

KEGG

ttj:TTHA1684.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

HBG244712.

Enzyme and pathway databases

BioCyc

TTHE300852:TTHA1684-MONOMER.

Family and domain databases

HAMAP

MF_00374_B. Ribosomal_L29_B.
[Tree]

InterPro

IPR001854. Ribosomal_L29.
IPR018254. Ribosomal_L29_CS.
[Graphical view]

Gene3D

G3DSA:1.10.287.310. Ribosomal_L29. 1 hit.

Pfam

PF00831. Ribosomal_L29. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00012. L29. 1 hit.

PROSITE

PS00579. RIBOSOMAL_L29. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RL29_THET8

Accession

Primary (citable) accession number: Q5SHP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	December 21, 2004
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)