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Protein

50S ribosomal protein L23

Gene

rplW

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome.UniRule annotation

GO - Molecular functioni

  1. nucleotide binding Source: InterPro
  2. rRNA binding Source: UniProtKB-HAMAP
  3. structural constituent of ribosome Source: InterPro

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1729-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L23UniRule annotation
Gene namesi
Name:rplWUniRule annotation
Ordered Locus Names:TTHA1690
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. ribosome Source: UniProtKB-KW
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 969650S ribosomal protein L23PRO_0000129427Add
BLAST

Interactioni

Subunit structurei

Contacts protein L29, and trigger factor when it is bound to the ribosome (By similarity). Part of the 50S ribosomal subunit.By similarity

Protein-protein interaction databases

STRINGi300852.TTHA1690.

Structurei

Secondary structure

1
96
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 74Combined sources
Beta strandi8 – 114Combined sources
Helixi15 – 184Combined sources
Helixi19 – 235Combined sources
Beta strandi25 – 306Combined sources
Beta strandi32 – 343Combined sources
Helixi36 – 4712Combined sources
Beta strandi51 – 599Combined sources
Beta strandi63 – 664Combined sources
Beta strandi69 – 724Combined sources
Beta strandi76 – 838Combined sources
Turni90 – 923Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VVJX-ray3.44RX/YX1-96[»]
1VY4X-ray2.60BX/DX1-96[»]
1VY5X-ray2.55BX/DX1-96[»]
1VY6X-ray2.90BX/DX1-96[»]
1VY7X-ray2.80BX/DX1-96[»]
4L47X-ray3.22RX/YX1-96[»]
4L71X-ray3.90RX/YX1-96[»]
4LELX-ray3.90RX/YX1-96[»]
4LFZX-ray3.92RX/YX1-96[»]
4LNTX-ray2.94RX/YX1-96[»]
4LSKX-ray3.48RX/YX1-96[»]
4LT8X-ray3.14RX/YX1-96[»]
4P6FX-ray3.60RX/YX1-96[»]
4P70X-ray3.68RX/YX1-96[»]
4V42X-ray5.50T1-96[»]
4V4PX-ray5.50T1-96[»]
4V51X-ray2.80BX/DX1-96[»]
4V5AX-ray3.50BX/DX1-96[»]
4V5CX-ray3.30BX/DX1-96[»]
4V5DX-ray3.50BX/DX1-96[»]
4V5EX-ray3.45BX/DX1-96[»]
4V5FX-ray3.60BX/DX1-96[»]
4V5GX-ray3.60BX/DX1-96[»]
4V5JX-ray3.10BX/DX1-96[»]
4V5KX-ray3.20BX/DX1-96[»]
4V5LX-ray3.10BX1-96[»]
4V5Melectron microscopy7.80BX1-96[»]
4V5Nelectron microscopy7.60BX1-96[»]
4V5PX-ray3.10BX/DX1-96[»]
4V5QX-ray3.10BX/DX1-96[»]
4V5RX-ray3.10BX/DX1-96[»]
4V5SX-ray3.10BX/DX1-96[»]
4V68electron microscopy6.40BX3-95[»]
4V6AX-ray3.10BX/DX1-96[»]
4V6FX-ray3.10AT/DT1-96[»]
4V6GX-ray3.50BT/DT1-96[»]
4V7JX-ray3.30AX/BX1-96[»]
4V7KX-ray3.60AX/BX1-96[»]
4V7LX-ray3.00BX/DX1-96[»]
4V7MX-ray3.45BX/DX1-96[»]
4V7WX-ray3.00BX/DX1-96[»]
4V7XX-ray3.00BX/DX1-96[»]
4V7YX-ray3.00BX/DX1-96[»]
4V7ZX-ray3.10BX/DX1-96[»]
4V87X-ray3.10AT/DT3-94[»]
4V8AX-ray3.20AX/BX1-96[»]
4V8BX-ray3.00BT/DT1-96[»]
4V8CX-ray3.30AT/BT1-96[»]
4V8DX-ray3.00BT/DT1-96[»]
4V8EX-ray3.30AT/CT1-96[»]
4V8FX-ray3.30AT/DT1-96[»]
4V8GX-ray3.00BX/DX1-96[»]
4V8HX-ray3.10BX/DX1-96[»]
4V8IX-ray2.70BX/DX1-96[»]
4V8JX-ray3.90BX/DX1-96[»]
4V8NX-ray3.10BX/DX1-96[»]
4V8OX-ray3.80BX1-96[»]
4V8QX-ray3.10AX1-96[»]
4V8UX-ray3.70BX/DX1-96[»]
4V8XX-ray3.35BX/DX1-96[»]
4V90X-ray2.95BX2-96[»]
4V95X-ray3.20BX/DX1-96[»]
4V97X-ray3.52BX/DX1-96[»]
4V9AX-ray3.30BT/DT1-96[»]
4V9BX-ray3.10BT/DT1-96[»]
4V9HX-ray2.86BX1-96[»]
4V9IX-ray3.30BX/DX3-94[»]
4V9RX-ray3.00BX/DX1-96[»]
4V9SX-ray3.10BX/DX1-96[»]
4W2EX-ray2.90X1-96[»]
4W2FX-ray2.40BX/DX1-96[»]
4W2GX-ray2.55BX/DX1-96[»]
4W2HX-ray2.70BX/DX1-96[»]
4W2IX-ray2.70BX/DX1-96[»]
SMRiQ5SHP0. Positions 1-96.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SHP0.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L23P family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0089.
HOGENOMiHOG000231366.
KOiK02892.
OMAiMIRRENN.
OrthoDBiEOG6HTP4P.
PhylomeDBiQ5SHP0.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
HAMAPiMF_01369_B. Ribosomal_L23_B.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR012678. Ribosomal_L23/L15e_core_dom.
IPR013025. Ribosomal_L25/23.
[Graphical view]
PfamiPF00276. Ribosomal_L23. 1 hit.
[Graphical view]
SUPFAMiSSF54189. SSF54189. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5SHP0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTAYDVILA PVLSEKAYAG FAEGKYTFWV HPKATKTEIK NAVETAFKVK
60 70 80 90
VVKVNTLHVR GKKKRLGRYL GKRPDRKKAI VQVAPGQKIE ALEGLI
Length:96
Mass (Da):10,737
Last modified:December 21, 2004 - v1
Checksum:i28ABA3C2D83A102A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181Y → R AA sequence (PubMed:11154066).Curated

Mass spectrometryi

Molecular mass is 10737 Da from positions 1 - 96. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71513.1.
RefSeqiYP_144956.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71513; BAD71513; BAD71513.
GeneIDi3168722.
KEGGittj:TTHA1690.
PATRICi23958335. VBITheThe93045_1660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71513.1.
RefSeqiYP_144956.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VVJX-ray3.44RX/YX1-96[»]
1VY4X-ray2.60BX/DX1-96[»]
1VY5X-ray2.55BX/DX1-96[»]
1VY6X-ray2.90BX/DX1-96[»]
1VY7X-ray2.80BX/DX1-96[»]
4L47X-ray3.22RX/YX1-96[»]
4L71X-ray3.90RX/YX1-96[»]
4LELX-ray3.90RX/YX1-96[»]
4LFZX-ray3.92RX/YX1-96[»]
4LNTX-ray2.94RX/YX1-96[»]
4LSKX-ray3.48RX/YX1-96[»]
4LT8X-ray3.14RX/YX1-96[»]
4P6FX-ray3.60RX/YX1-96[»]
4P70X-ray3.68RX/YX1-96[»]
4V42X-ray5.50T1-96[»]
4V4PX-ray5.50T1-96[»]
4V51X-ray2.80BX/DX1-96[»]
4V5AX-ray3.50BX/DX1-96[»]
4V5CX-ray3.30BX/DX1-96[»]
4V5DX-ray3.50BX/DX1-96[»]
4V5EX-ray3.45BX/DX1-96[»]
4V5FX-ray3.60BX/DX1-96[»]
4V5GX-ray3.60BX/DX1-96[»]
4V5JX-ray3.10BX/DX1-96[»]
4V5KX-ray3.20BX/DX1-96[»]
4V5LX-ray3.10BX1-96[»]
4V5Melectron microscopy7.80BX1-96[»]
4V5Nelectron microscopy7.60BX1-96[»]
4V5PX-ray3.10BX/DX1-96[»]
4V5QX-ray3.10BX/DX1-96[»]
4V5RX-ray3.10BX/DX1-96[»]
4V5SX-ray3.10BX/DX1-96[»]
4V68electron microscopy6.40BX3-95[»]
4V6AX-ray3.10BX/DX1-96[»]
4V6FX-ray3.10AT/DT1-96[»]
4V6GX-ray3.50BT/DT1-96[»]
4V7JX-ray3.30AX/BX1-96[»]
4V7KX-ray3.60AX/BX1-96[»]
4V7LX-ray3.00BX/DX1-96[»]
4V7MX-ray3.45BX/DX1-96[»]
4V7WX-ray3.00BX/DX1-96[»]
4V7XX-ray3.00BX/DX1-96[»]
4V7YX-ray3.00BX/DX1-96[»]
4V7ZX-ray3.10BX/DX1-96[»]
4V87X-ray3.10AT/DT3-94[»]
4V8AX-ray3.20AX/BX1-96[»]
4V8BX-ray3.00BT/DT1-96[»]
4V8CX-ray3.30AT/BT1-96[»]
4V8DX-ray3.00BT/DT1-96[»]
4V8EX-ray3.30AT/CT1-96[»]
4V8FX-ray3.30AT/DT1-96[»]
4V8GX-ray3.00BX/DX1-96[»]
4V8HX-ray3.10BX/DX1-96[»]
4V8IX-ray2.70BX/DX1-96[»]
4V8JX-ray3.90BX/DX1-96[»]
4V8NX-ray3.10BX/DX1-96[»]
4V8OX-ray3.80BX1-96[»]
4V8QX-ray3.10AX1-96[»]
4V8UX-ray3.70BX/DX1-96[»]
4V8XX-ray3.35BX/DX1-96[»]
4V90X-ray2.95BX2-96[»]
4V95X-ray3.20BX/DX1-96[»]
4V97X-ray3.52BX/DX1-96[»]
4V9AX-ray3.30BT/DT1-96[»]
4V9BX-ray3.10BT/DT1-96[»]
4V9HX-ray2.86BX1-96[»]
4V9IX-ray3.30BX/DX3-94[»]
4V9RX-ray3.00BX/DX1-96[»]
4V9SX-ray3.10BX/DX1-96[»]
4W2EX-ray2.90X1-96[»]
4W2FX-ray2.40BX/DX1-96[»]
4W2GX-ray2.55BX/DX1-96[»]
4W2HX-ray2.70BX/DX1-96[»]
4W2IX-ray2.70BX/DX1-96[»]
SMRiQ5SHP0. Positions 1-96.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1690.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71513; BAD71513; BAD71513.
GeneIDi3168722.
KEGGittj:TTHA1690.
PATRICi23958335. VBITheThe93045_1660.

Phylogenomic databases

eggNOGiCOG0089.
HOGENOMiHOG000231366.
KOiK02892.
OMAiMIRRENN.
OrthoDBiEOG6HTP4P.
PhylomeDBiQ5SHP0.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1729-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ5SHP0.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
HAMAPiMF_01369_B. Ribosomal_L23_B.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR012678. Ribosomal_L23/L15e_core_dom.
IPR013025. Ribosomal_L25/23.
[Graphical view]
PfamiPF00276. Ribosomal_L23. 1 hit.
[Graphical view]
SUPFAMiSSF54189. SSF54189. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.
  2. "Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus."
    Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T.
    Biol. Chem. 381:1079-1087(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-23.
  3. "Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry."
    Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A.
    Proteomics 5:4818-4831(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: MASS SPECTROMETRY.
  4. "The path of messenger RNA through the ribosome."
    Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F.
    Cell 106:233-241(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
  5. Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME.

Entry informationi

Entry nameiRL23_THET8
AccessioniPrimary (citable) accession number: Q5SHP0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: December 21, 2004
Last modified: April 1, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.