Reviewed, UniProtKB/Swiss-Prot Q5SHP0 (RL23_THET8)

Names and origin

Protein names

Recommended name:
    50S ribosomal protein L23

Gene names

Name:	rplW
Ordered Locus Names:	TTHA1690

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Complete proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	96 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	One of the early assembly proteins By similarity it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome By similarity. HAMAP MF_01369
Subunit structure	Contacts protein L29, and trigger factor when it is bound to the ribosome By similarity. Part of the 50S ribosomal subunit. HAMAP MF_01369
Sequence similarities	Belongs to the ribosomal protein L23P family.
Mass spectrometry	Molecular mass is 10737 Da from positions 1 - 96. Determined by MALDI. Ref.3

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 96

96

50S ribosomal protein L23 HAMAP MF_01369

PRO_0000129427

Experimental info

Sequence conflict

18

1

Y → R AA sequence Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

96

Helix Strand Turn

Details...

Helix

15 – 18

4

Beta strand

32 – 34

3

Helix

40 – 43

4

Turn

44 – 48

5

Beta strand

89 – 91

3

Sequences

	Sequence		Length	Mass (Da)
	Q5SHP0-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 28ABA3C2D83A102A	FASTA	96	10,737
	10 20 30 40 50 60 MKTAYDVILA PVLSEKAYAG FAEGKYTFWV HPKATKTEIK NAVETAFKVK VVKVNTLHVR 70 80 90 GKKKRLGRYL GKRPDRKKAI VQVAPGQKIE ALEGLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus." Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T. Biol. Chem. 381:1079-1087(2000) [PubMed: 11154066] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-23.
[3]	"Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry." Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A. Proteomics 5:4818-4831(2005) [PubMed: 16287167] [Abstract] Cited for: MASS SPECTROMETRY.
[4]	"The path of messenger RNA through the ribosome." Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F. Cell 106:233-241(2001) [PubMed: 11511350] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
[5]	"Crystal structure of the ribosome at 5.5 A resolution." Yusupov M.M., Yusupova G.Z., Baucom A., Lieberman K., Earnest T.N., Cate J.H.D., Noller H.F. Science 292:883-896(2001) [PubMed: 11283358] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AP008226 Genomic DNA. Translation: BAD71513.1.

RefSeq

YP_144956.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GIY	X-ray	5.50	T	1-96	[»]
1YL3	X-ray	5.50	T	1-96	[»]
2J01	X-ray	2.80	X	1-96	[»]
2J03	X-ray	2.80	X	1-96	[»]
2V47	X-ray	3.80	X	1-96	[»]
2V49	X-ray	3.80	X	1-96	[»]
2WDI	X-ray	3.30	X	1-96	[»]
2WDJ	X-ray	3.30	X	1-96	[»]
2WDL	X-ray	3.50	X	1-96	[»]
2WDN	X-ray	3.50	X	1-96	[»]
2WH2	X-ray	3.45	X	1-96	[»]
2WH4	X-ray	3.45	X	1-96	[»]
2WRJ	X-ray	3.60	X	1-96	[»]
2WRL	X-ray	3.60	X	1-96	[»]
2WRO	X-ray	3.60	X	1-96	[»]
2WRR	X-ray	3.60	X	1-96	[»]
3FIN	electron microscopy	6.40	X	3-95	[»]
3HUX	X-ray	3.10	X	1-96	[»]
3HUZ	X-ray	3.10	X	1-96	[»]
3KIR	X-ray	3.30	X	1-96	[»]
3KIT	X-ray	3.30	X	1-96	[»]
3KIW	X-ray	3.60	X	1-96	[»]
3KIY	X-ray	3.60	X	1-96	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q5SHP0.

Genome annotation databases

GeneID

3168722.

GenomeReviews

Gene locus TTHA1690 in contig AP008226_GR.

KEGG

ttj:TTHA1690.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0089.

HOGENOM

HBG439330.

OMA

NTINRQG.

Enzyme and pathway databases

BioCyc

TTHE300852:TTHA1690-MONOMER.

Family and domain databases

HAMAP

MF_01369_B. Ribosomal_L23_B.
[Tree]

InterPro

IPR012677. a_b_plait_nuc_bd.
IPR012678. Ribosomal_L23/L15e_core.
IPR013025. Ribosomal_L25/23.
[Graphical view]

Gene3D

G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.

Pfam

PF00276. Ribosomal_L23. 1 hit.
[Graphical view]

PROSITE

PS00050. RIBOSOMAL_L23. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RL23_THET8

Accession

Primary (citable) accession number: Q5SHP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	December 21, 2004
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)