50S ribosomal protein L3 - Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)

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Reviewed, UniProtKB/Swiss-Prot Q5SHN8 (RL3_THET8)

Last modified November 3, 2009. Version 38. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
50S ribosomal protein L3

Gene names

Name:	rplC
Ordered Locus Names:	TTHA1692

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit By similarity.
Subunit structure	Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L19. HAMAP MF_01325
Post-translational modification	The mass differences between the observed and calculated masses are suggested to be due to methylation, which is known to occur in the E.coli and R.palustris orthologs. HAMAP MF_01325
Sequence similarities	Belongs to the ribosomal protein L3P family.
Mass spectrometry	Molecular mass is 22437 Da from positions 1 - 206. Determined by MALDI. Ref.3

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Methylation
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

206

50S ribosomal protein L3 HAMAP MF_01325

PRO_0000077180

Secondary structure

1

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206

Helix Strand Turn

Sequences

Sequence

Length

Mass (Da)

Tools

Q5SHN8-1 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: A920741478CB6028
Show »

206

22,408

        10         20         30         40         50         60 
MKGILGVKVG MTRIFRDDRA VPVTVILAGP CPVVQRRTPE KDGYTAVQLG FLPQNPKRVN 

        70         80         90        100        110        120 
RPLKGHFAKA GVEPVRILRE IRDFNPEGDT VTVEIFKPGE RVDVTGTSKG RGFAGVMKRW 

       130        140        150        160        170        180 
NFAGGPDSHG AHKIHRHPGS IGNRKTPGRV YKGKKMAGHY GAERVTVMNL EVVDVIPEEN 

       190        200 
LLLVKGAVPG PNGGLVIVRE TKKAAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus." Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T. Biol. Chem. 381:1079-1087(2000) [PubMed: 11154066] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-21.
[3]	"Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry." Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A. Proteomics 5:4818-4831(2005) [PubMed: 16287167] [Abstract] Cited for: MASS SPECTROMETRY.
[4]	"The path of messenger RNA through the ribosome." Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F. Cell 106:233-241(2001) [PubMed: 11511350] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
[5]	"Crystal structure of the ribosome at 5.5 A resolution." Yusupov M.M., Yusupova G.Z., Baucom A., Lieberman K., Earnest T.N., Cate J.H.D., Noller H.F. Science 292:883-896(2001) [PubMed: 11283358] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME.
+	Additional computationally mapped references.

Cross-references

Sequence databases

AP008226 Genomic DNA. Translation: BAD71515.1.

RefSeq

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GIY	X-ray	5.50	E	-	[»]
1YL3	X-ray	5.50	E	183-191	[»]
2HGJ	X-ray	5.00	E	-	[»]
2HGQ	X-ray	5.50	E	1-206	[»]
2HGU	X-ray	4.51	E	-	[»]
2J01	X-ray	2.80	E	1-206	[»]
2J03	X-ray	2.80	E	1-206	[»]
2V47	X-ray	3.80	E	1-206	[»]
2V49	X-ray	3.80	E	1-206	[»]
2WDI	X-ray	3.30	E	1-206	[»]
2WDJ	X-ray	3.30	E	1-206	[»]
2WDL	X-ray	3.50	E	1-206	[»]
2WDN	X-ray	3.50	E	1-206	[»]
2WH2	X-ray	3.45	E	1-206	[»]
2WH4	X-ray	3.45	E	1-206	[»]
3FIN	electron microscopy	6.40	E	1-205	[»]

ModBase

Protein-protein interaction databases

STRING

Genome annotation databases

GeneID

GenomeReviews

Gene locus TTHA1692 in contig AP008226_GR.

KEGG

Organism-specific databases

CMR

Phylogenomic databases

HOGENOM

OMA

Enzyme and pathway databases

BioCyc

TTHE300852:TTHA1692-MON.

Family and domain databases

HAMAP

MF_01325.
[Tree]

InterPro

IPR000597. Ribosomal_L3.
IPR019927. Ribosomal_L3_bac/org-type.
IPR019926. Ribosomal_L3_CS.
[Graphical view]

Pfam

PF00297. Ribosomal_L3. 1 hit.
[Graphical view]

ProDom

PD001374. Ribosomal_L3. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR03625. L3_bact. 1 hit.

PROSITE

PS00474. RIBOSOMAL_L3. 1 hit.
[Graphical view]

ProtoNet

Entry information

Entry name

RL3_THET8

Accession

Primary (citable) accession number: Q5SHN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	December 21, 2004
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Ribosomal proteins

Ribosomal proteins families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents