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Q5SHN8 (RL3_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
50S ribosomal protein L3
Gene names
Name:rplC
Ordered Locus Names:TTHA1692
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length206 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit By similarity. HAMAP-Rule MF_01325_B

Subunit structure

Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L19.

Post-translational modification

The mass differences between the observed and calculated masses are suggested to be due to methylation, which is known to occur in the E.coli and R.palustris orthologs. HAMAP-Rule MF_01325_B

Sequence similarities

Belongs to the ribosomal protein L3P family.

Mass spectrometry

Molecular mass is 22437 Da from positions 1 - 206. Determined by MALDI. Ref.3

Ontologies

Keywords
   LigandRNA-binding
rRNA-binding
   Molecular functionRibonucleoprotein
Ribosomal protein
   PTMMethylation
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentribosome

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionrRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

structural constituent of ribosome

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 20620650S ribosomal protein L3 HAMAP-Rule MF_01325_B
PRO_0000077180

Secondary structure

............................................... 206
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q5SHN8 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: A920741478CB6028

FASTA20622,408
        10         20         30         40         50         60 
MKGILGVKVG MTRIFRDDRA VPVTVILAGP CPVVQRRTPE KDGYTAVQLG FLPQNPKRVN 

        70         80         90        100        110        120 
RPLKGHFAKA GVEPVRILRE IRDFNPEGDT VTVEIFKPGE RVDVTGTSKG RGFAGVMKRW 

       130        140        150        160        170        180 
NFAGGPDSHG AHKIHRHPGS IGNRKTPGRV YKGKKMAGHY GAERVTVMNL EVVDVIPEEN 

       190        200 
LLLVKGAVPG PNGGLVIVRE TKKAAK 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
[2]"Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus."
Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T.
Biol. Chem. 381:1079-1087(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-21.
[3]"Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry."
Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A.
Proteomics 5:4818-4831(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY.
[4]"The path of messenger RNA through the ribosome."
Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F.
Cell 106:233-241(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
[5]"Crystal structure of the ribosome at 5.5 A resolution."
Yusupov M.M., Yusupova G.Z., Baucom A., Lieberman K., Earnest T.N., Cate J.H.D., Noller H.F.
Science 292:883-896(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008226 Genomic DNA. Translation: BAD71515.1.
RefSeqYP_144958.1. NC_006461.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GIYX-ray5.50E21-26[»]
1YL3X-ray5.50E21-26[»]
2HGJX-ray5.00E1-206[»]
2HGQX-ray5.50E1-206[»]
2HGUX-ray4.51E1-206[»]
2J01X-ray2.80E1-206[»]
2J03X-ray2.80E1-206[»]
2V47X-ray3.80E1-206[»]
2V49X-ray3.80E1-206[»]
2WDIX-ray3.30E1-206[»]
2WDJX-ray3.30E1-206[»]
2WDLX-ray3.50E1-206[»]
2WDNX-ray3.50E1-206[»]
2WH2X-ray3.45E1-206[»]
2WH4X-ray3.45E1-206[»]
2WRJX-ray3.60E1-206[»]
2WRLX-ray3.60E1-206[»]
2WROX-ray3.60E1-206[»]
2WRRX-ray3.60E1-206[»]
2X9SX-ray3.10E1-206[»]
2X9UX-ray3.10E1-206[»]
2XG0X-ray3.20E1-206[»]
2XG2X-ray3.20E1-206[»]
2XQEX-ray3.10E1-206[»]
2XTGelectron microscopy7.80E1-206[»]
2XUXelectron microscopy7.60E1-206[»]
2Y0VX-ray3.10E1-206[»]
2Y0XX-ray3.10E1-206[»]
2Y0ZX-ray3.10E1-206[»]
2Y11X-ray3.10E1-206[»]
2Y13X-ray3.10E1-206[»]
2Y15X-ray3.10E1-206[»]
2Y17X-ray3.10E1-206[»]
2Y19X-ray3.10E1-206[»]
3FINelectron microscopy6.40E1-205[»]
3HUXX-ray3.10E1-206[»]
3HUZX-ray3.10E1-206[»]
3I8FX-ray3.10E1-206[»]
3I8IX-ray3.10E1-206[»]
3I9CX-ray3.50E1-206[»]
3I9EX-ray3.50E1-206[»]
3KIRX-ray3.30E1-206[»]
3KITX-ray3.30E1-206[»]
3KIWX-ray3.60E1-206[»]
3KIYX-ray3.60E1-206[»]
3KNIX-ray3.00E1-206[»]
3KNKX-ray3.00E1-206[»]
3KNMX-ray3.45E1-206[»]
3KNOX-ray3.45E1-206[»]
3OH5X-ray3.00E1-206[»]
3OH7X-ray3.00E1-206[»]
3OHJX-ray3.00E1-206[»]
3OHKX-ray3.00E1-206[»]
3OHZX-ray3.00E1-206[»]
3OI1X-ray3.00E1-206[»]
3OI3X-ray3.10E1-206[»]
3OI5X-ray3.10E1-206[»]
3TVEX-ray3.10E1-205[»]
3TVHX-ray3.10E1-205[»]
3UXQX-ray3.20E1-206[»]
3UXRX-ray3.20E1-206[»]
3UYEX-ray3.00E1-206[»]
3UYGX-ray3.00E1-206[»]
3UZ1X-ray3.30E1-206[»]
3UZ2X-ray3.30E1-206[»]
3UZ8X-ray3.00E1-206[»]
3UZ9X-ray3.00E1-206[»]
3UZFX-ray3.30E1-206[»]
3UZHX-ray3.30E1-206[»]
3UZKX-ray3.30E1-206[»]
3UZNX-ray3.30E1-206[»]
3V23X-ray3.00E1-206[»]
3V25X-ray3.00E1-206[»]
3V27X-ray3.10E1-206[»]
3V29X-ray3.10E1-206[»]
3V2DX-ray2.70E1-206[»]
3V2FX-ray2.70E1-206[»]
3V6WX-ray3.90E1-206[»]
3V6XX-ray3.90E1-206[»]
3ZN9X-ray3.10E1-206[»]
3ZNEX-ray3.10E1-206[»]
3ZVPX-ray3.80E1-206[»]
4ABSX-ray3.10E1-206[»]
4B8GX-ray3.70E1-206[»]
4B8IX-ray3.70E1-206[»]
4BTDX-ray2.95E1-206[»]
4BYCX-ray3.35E1-206[»]
4BYEX-ray3.35E1-206[»]
4DHAX-ray3.20E1-206[»]
4DHCX-ray3.20E1-206[»]
4EJBX-ray3.52E1-206[»]
4EJCX-ray3.52E1-206[»]
4G5LX-ray3.30E1-206[»]
4G5NX-ray3.30E1-206[»]
4G5UX-ray3.10E1-206[»]
4G5WX-ray3.10E1-206[»]
4JUXX-ray2.86E1-206[»]
4K0MX-ray3.30E1-204[»]
4K0QX-ray3.30E1-204[»]
4NVVX-ray3.00E1-206[»]
4NVXX-ray3.00E1-206[»]
4NVZX-ray3.10E1-206[»]
4NW1X-ray3.10E1-206[»]
ProteinModelPortalQ5SHN8.
SMRQ5SHN8. Positions 1-206.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING300852.TTHA1692.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD71515; BAD71515; BAD71515.
GeneID3167929.
KEGGttj:TTHA1692.
PATRIC23958339. VBITheThe93045_1662.

Phylogenomic databases

eggNOGCOG0087.
HOGENOMHOG000100368.
KOK02906.
OMASMQDATH.
OrthoDBEOG6WDSMH.
PhylomeDBQ5SHN8.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-1731-MONOMER.

Family and domain databases

HAMAPMF_01325_B. Ribosomal_L3_B.
InterProIPR000597. Ribosomal_L3.
IPR019927. Ribosomal_L3_bac/org-type.
IPR019926. Ribosomal_L3_CS.
IPR009000. Transl_B-barrel.
[Graphical view]
PANTHERPTHR11229. PTHR11229. 1 hit.
PfamPF00297. Ribosomal_L3. 1 hit.
[Graphical view]
SUPFAMSSF50447. SSF50447. 1 hit.
TIGRFAMsTIGR03625. L3_bact. 1 hit.
PROSITEPS00474. RIBOSOMAL_L3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ5SHN8.

Entry information

Entry nameRL3_THET8
AccessionPrimary (citable) accession number: Q5SHN8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: December 21, 2004
Last modified: July 9, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references