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Protein

Elongation factor G

Gene

fusA

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 26GTPBy similarity8
Nucleotide bindingi83 – 87GTPBy similarity5
Nucleotide bindingi137 – 140GTPBy similarity4

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor G
Short name:
EF-G
Gene namesi
Name:fusA
Synonyms:fus
Ordered Locus Names:TTHA1695
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000912491 – 691Elongation factor GAdd BLAST691

Interactioni

Protein-protein interaction databases

STRINGi300852.TTHA1695.

Structurei

Secondary structure

1691
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 11Combined sources3
Beta strandi12 – 19Combined sources8
Helixi21 – 24Combined sources4
Helixi25 – 36Combined sources12
Beta strandi69 – 74Combined sources6
Beta strandi77 – 82Combined sources6
Helixi91 – 100Combined sources10
Beta strandi102 – 109Combined sources8
Turni110 – 112Combined sources3
Helixi116 – 127Combined sources12
Beta strandi132 – 137Combined sources6
Helixi146 – 155Combined sources10
Beta strandi161 – 163Combined sources3
Beta strandi165 – 169Combined sources5
Helixi171 – 173Combined sources3
Beta strandi176 – 179Combined sources4
Turni180 – 183Combined sources4
Beta strandi184 – 188Combined sources5
Beta strandi190 – 194Combined sources5
Beta strandi196 – 199Combined sources4
Helixi203 – 205Combined sources3
Helixi206 – 220Combined sources15
Turni221 – 223Combined sources3
Helixi225 – 233Combined sources9
Helixi239 – 251Combined sources13
Beta strandi256 – 260Combined sources5
Helixi263 – 265Combined sources3
Helixi269 – 279Combined sources11
Turni283 – 285Combined sources3
Beta strandi289 – 292Combined sources4
Beta strandi294 – 296Combined sources3
Beta strandi298 – 301Combined sources4
Beta strandi310 – 319Combined sources10
Turni320 – 322Combined sources3
Beta strandi323 – 338Combined sources16
Beta strandi340 – 343Combined sources4
Turni344 – 346Combined sources3
Beta strandi349 – 351Combined sources3
Beta strandi354 – 358Combined sources5
Beta strandi363 – 370Combined sources8
Beta strandi374 – 378Combined sources5
Beta strandi388 – 391Combined sources4
Turni426 – 433Combined sources8
Beta strandi436 – 438Combined sources3
Beta strandi439 – 441Combined sources3
Beta strandi449 – 454Combined sources6
Helixi458 – 463Combined sources6
Beta strandi464 – 467Combined sources4
Beta strandi470 – 473Combined sources4
Turni474 – 476Combined sources3
Beta strandi484 – 486Combined sources3
Beta strandi491 – 499Combined sources9
Beta strandi502 – 504Combined sources3
Beta strandi506 – 516Combined sources11
Beta strandi523 – 527Combined sources5
Turni536 – 538Combined sources3
Helixi539 – 549Combined sources11
Turni554 – 556Combined sources3
Beta strandi562 – 570Combined sources9
Turni574 – 576Combined sources3
Helixi579 – 595Combined sources17
Beta strandi600 – 612Combined sources13
Turni614 – 618Combined sources5
Helixi619 – 626Combined sources8
Beta strandi631 – 637Combined sources7
Beta strandi640 – 648Combined sources9
Turni649 – 651Combined sources3
Beta strandi652 – 654Combined sources3
Helixi655 – 662Combined sources8
Turni663 – 665Combined sources3
Beta strandi668 – 678Combined sources11
Helixi681 – 688Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DARX-ray2.40A1-691[»]
1EFGX-ray2.70A1-691[»]
1ELOX-ray2.80A1-691[»]
1ZN0electron microscopy15.50B6-688[»]
2EFGX-ray2.60A1-691[»]
4V5FX-ray3.60AY/CY1-691[»]
4V5Melectron microscopy7.80AY1-691[»]
4V5Nelectron microscopy7.60AY1-691[»]
4V8UX-ray3.70AY/CY1-691[»]
4V90X-ray2.95AY1-691[»]
4V9HX-ray2.86AY10-689[»]
4WPOX-ray2.80BZ/DZ8-691[»]
4WQFX-ray2.80BZ/DZ8-691[»]
4WQUX-ray2.80BZ/DZ8-691[»]
4WQYX-ray2.80BZ/DZ8-691[»]
5HAUX-ray3.001z/2z8-691[»]
ProteinModelPortaliQ5SHN5.
SMRiQ5SHN5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SHN5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 284tr-type GAdd BLAST275

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CEJ. Bacteria.
COG0480. LUCA.
HOGENOMiHOG000231587.
KOiK02355.
OMAiKLGVAIQ.
PhylomeDBiQ5SHN5.

Family and domain databases

CDDicd01434. EFG_mtEFG1_IV. 1 hit.
Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00054_B. EF_G_EF_2_B. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004540. Transl_elong_EFG/EF2.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00484. EF-G. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SHN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVKVEYDLK RLRNIGIAAH IDAGKTTTTE RILYYTGRIH KIGEVHEGAA
60 70 80 90 100
TMDFMEQERE RGITITAAVT TCFWKDHRIN IIDTPGHVDF TIEVERSMRV
110 120 130 140 150
LDGAIVVFDS SQGVEPQSET VWRQAEKYKV PRIAFANKMD KTGADLWLVI
160 170 180 190 200
RTMQERLGAR PVVMQLPIGR EDTFSGIIDV LRMKAYTYGN DLGTDIREIP
210 220 230 240 250
IPEEYLDQAR EYHEKLVEVA ADFDENIMLK YLEGEEPTEE ELVAAIRKGT
260 270 280 290 300
IDLKITPVFL GSALKNKGVQ LLLDAVVDYL PSPLDIPPIK GTTPEGEVVE
310 320 330 340 350
IHPDPNGPLA ALAFKIMADP YVGRLTFIRV YSGTLTSGSY VYNTTKGRKE
360 370 380 390 400
RVARLLRMHA NHREEVEELK AGDLGAVVGL KETITGDTLV GEDAPRVILE
410 420 430 440 450
SIEVPEPVID VAIEPKTKAD QEKLSQALAR LAEEDPTFRV STHPETGQTI
460 470 480 490 500
ISGMGELHLE IIVDRLKREF KVDANVGKPQ VAYRETITKP VDVEGKFIRQ
510 520 530 540 550
TGGRGQYGHV KIKVEPLPRG SGFEFVNAIV GGVIPKEYIP AVQKGIEEAM
560 570 580 590 600
QSGPLIGFPV VDIKVTLYDG SYHEVDSSEM AFKIAGSMAI KEAVQKGDPV
610 620 630 640 650
ILEPIMRVEV TTPEEYMGDV IGDLNARRGQ ILGMEPRGNA QVIRAFVPLA
660 670 680 690
EMFGYATDLR SKTQGRGSFV MFFDHYQEVP KQVQEKLIKG Q
Length:691
Mass (Da):76,879
Last modified:December 21, 2004 - v1
Checksum:i8F0063EE8123470E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16278 Genomic DNA. Translation: CAA34354.1.
AP008226 Genomic DNA. Translation: BAD71518.1.
X52165 Genomic DNA. Translation: CAA36420.1.
PIRiS15928. EFTWG.
RefSeqiWP_011228848.1. NC_006461.1.
YP_144961.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71518; BAD71518; BAD71518.
GeneIDi3169680.
KEGGittj:TTHA1695.
PATRICi23958345. VBITheThe93045_1665.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16278 Genomic DNA. Translation: CAA34354.1.
AP008226 Genomic DNA. Translation: BAD71518.1.
X52165 Genomic DNA. Translation: CAA36420.1.
PIRiS15928. EFTWG.
RefSeqiWP_011228848.1. NC_006461.1.
YP_144961.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DARX-ray2.40A1-691[»]
1EFGX-ray2.70A1-691[»]
1ELOX-ray2.80A1-691[»]
1ZN0electron microscopy15.50B6-688[»]
2EFGX-ray2.60A1-691[»]
4V5FX-ray3.60AY/CY1-691[»]
4V5Melectron microscopy7.80AY1-691[»]
4V5Nelectron microscopy7.60AY1-691[»]
4V8UX-ray3.70AY/CY1-691[»]
4V90X-ray2.95AY1-691[»]
4V9HX-ray2.86AY10-689[»]
4WPOX-ray2.80BZ/DZ8-691[»]
4WQFX-ray2.80BZ/DZ8-691[»]
4WQUX-ray2.80BZ/DZ8-691[»]
4WQYX-ray2.80BZ/DZ8-691[»]
5HAUX-ray3.001z/2z8-691[»]
ProteinModelPortaliQ5SHN5.
SMRiQ5SHN5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1695.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71518; BAD71518; BAD71518.
GeneIDi3169680.
KEGGittj:TTHA1695.
PATRICi23958345. VBITheThe93045_1665.

Phylogenomic databases

eggNOGiENOG4105CEJ. Bacteria.
COG0480. LUCA.
HOGENOMiHOG000231587.
KOiK02355.
OMAiKLGVAIQ.
PhylomeDBiQ5SHN5.

Miscellaneous databases

EvolutionaryTraceiQ5SHN5.
PROiQ5SHN5.

Family and domain databases

CDDicd01434. EFG_mtEFG1_IV. 1 hit.
Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00054_B. EF_G_EF_2_B. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004540. Transl_elong_EFG/EF2.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00484. EF-G. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEFG_THET8
AccessioniPrimary (citable) accession number: Q5SHN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.