Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Acetylornithine/acetyl-lysine aminotransferase

Gene

argD

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in both the arginine and lysine biosynthetic pathways.By similarity

Catalytic activityi

N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate.
N(2)-acetyl-L-lysine + 2-oxoglutarate = N-acetyl-L-aminoadipate semialdehyde + L-glutamate.

Cofactori

pyridoxal 5'-phosphateNote: Binds 1 pyridoxal phosphate per subunit.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Arginine biosynthesis bifunctional protein ArgJ (argJ)
  2. Acetylglutamate kinase (TTHA1903), Acetylglutamate/acetylaminoadipate kinase (argB)
  3. N-acetyl-gamma-glutamyl-phosphate/N-acetyl-gamma-aminoadipyl-phosphate reductase (argC), N-acetyl-gamma-glutamyl-phosphate/N-acetyl-gamma-aminoadipyl-phosphate reductase (argC)
  4. Acetylornithine/acetyl-lysine aminotransferase (argD), Acetylornithine/acetyl-lysine aminotransferase (argD)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-lysine biosynthesis via AAA pathway

This protein is involved in step 4 of the subpathway that synthesizes L-lysine from L-alpha-aminoadipate (Thermus route).
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Alpha-aminoadipate--LysW ligase LysX (lysX)
  2. Acetylglutamate/acetylaminoadipate kinase (argB)
  3. N-acetyl-gamma-glutamyl-phosphate/N-acetyl-gamma-aminoadipyl-phosphate reductase (argC), N-acetyl-gamma-glutamyl-phosphate/N-acetyl-gamma-aminoadipyl-phosphate reductase (argC)
  4. Acetylornithine/acetyl-lysine aminotransferase (argD), Acetylornithine/acetyl-lysine aminotransferase (argD)
  5. N-acetyl-lysine deacetylase (lysK)
This subpathway is part of the pathway L-lysine biosynthesis via AAA pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-lysine from L-alpha-aminoadipate (Thermus route), the pathway L-lysine biosynthesis via AAA pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei140Pyridoxal phosphate; via carbonyl oxygenBy similarity1
Binding sitei143N2-acetyl-L-ornithine1
Binding sitei282N2-acetyl-L-ornithine1
Binding sitei283Pyridoxal phosphate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Lysine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00033; UER00038.
UPA00068; UER00109.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylornithine/acetyl-lysine aminotransferase (EC:2.6.1.-, EC:2.6.1.11)
Short name:
ACOAT
Gene namesi
Name:argD
Synonyms:lysJ
Ordered Locus Names:TTHA1755
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003112621 – 395Acetylornithine/acetyl-lysine aminotransferaseAdd BLAST395

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei254N6-(pyridoxal phosphate)lysine1

Interactioni

Subunit structurei

Homodimer.Curated

Protein-protein interaction databases

STRINGi300852.TTHA1755.

Structurei

Secondary structure

1395
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 19Combined sources10
Beta strandi31 – 36Combined sources6
Beta strandi38 – 41Combined sources4
Beta strandi46 – 51Combined sources6
Helixi52 – 55Combined sources4
Helixi64 – 76Combined sources13
Helixi87 – 98Combined sources12
Beta strandi104 – 112Combined sources9
Helixi113 – 128Combined sources16
Beta strandi132 – 136Combined sources5
Helixi145 – 149Combined sources5
Helixi154 – 157Combined sources4
Helixi158 – 160Combined sources3
Beta strandi167 – 170Combined sources4
Helixi175 – 181Combined sources7
Beta strandi186 – 191Combined sources6
Beta strandi193 – 195Combined sources3
Turni196 – 199Combined sources4
Beta strandi200 – 202Combined sources3
Helixi205 – 218Combined sources14
Beta strandi221 – 225Combined sources5
Turni227 – 234Combined sources8
Beta strandi235 – 238Combined sources4
Helixi240 – 243Combined sources4
Beta strandi248 – 252Combined sources5
Helixi254 – 257Combined sources4
Beta strandi263 – 268Combined sources6
Helixi269 – 273Combined sources5
Helixi288 – 304Combined sources17
Helixi307 – 322Combined sources16
Beta strandi329 – 335Combined sources7
Beta strandi338 – 345Combined sources8
Helixi348 – 358Combined sources11
Beta strandi363 – 366Combined sources4
Beta strandi369 – 372Combined sources4
Helixi380 – 394Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VEFX-ray1.35A/B1-395[»]
1WKGX-ray2.25A/B1-395[»]
1WKHX-ray2.25A/B1-395[»]
ProteinModelPortaliQ5SHH5.
SMRiQ5SHH5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SHH5.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni113 – 114Pyridoxal phosphate binding2
Regioni225 – 228Pyridoxal phosphate binding4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C8Y. Bacteria.
COG4992. LUCA.
HOGENOMiHOG000020206.
KOiK05830.
OMAiFSEIRGM.
PhylomeDBiQ5SHH5.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPiMF_01107. ArgD_aminotrans_3. 1 hit.
InterProiIPR004636. AcOrn/SuccOrn_fam.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00707. argD. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SHH5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METRTLEDWR ALLEAEKTLD SGVYNKHDLL IVRGQGARVW DAEGNEYIDC
60 70 80 90 100
VGGYGVANLG HGNPEVVEAV KRQAETLMAM PQTLPTPMRG EFYRTLTAIL
110 120 130 140 150
PPELNRVFPV NSGTEANEAA LKFARAHTGR KKFVAAMRGF SGRTMGSLSV
160 170 180 190 200
TWEPKYREPF LPLVEPVEFI PYNDVEALKR AVDEETAAVI LEPVQGEGGV
210 220 230 240 250
RPATPEFLRA AREITQEKGA LLILDEIQTG MGRTGKRFAF EHFGIVPDIL
260 270 280 290 300
TLAKALGGGV PLGAAVMREE VARSMPKGGH GTTFGGNPLA MAAGVAAIRY
310 320 330 340 350
LERTRLWERA AELGPWFMEK LRAIPSPKIR EVRGMGLMVG LELKEKAAPY
360 370 380 390
IARLEKEHRV LALQAGPTVI RFLPPLVIEK EDLERVVEAV RAVLA
Length:395
Mass (Da):43,423
Last modified:December 21, 2004 - v1
Checksum:i1D3B73EAC3454C3A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71578.1.
RefSeqiWP_011228893.1. NC_006461.1.
YP_145021.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71578; BAD71578; BAD71578.
GeneIDi3169449.
KEGGittj:TTHA1755.
PATRICi23958473. VBITheThe93045_1726.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71578.1.
RefSeqiWP_011228893.1. NC_006461.1.
YP_145021.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VEFX-ray1.35A/B1-395[»]
1WKGX-ray2.25A/B1-395[»]
1WKHX-ray2.25A/B1-395[»]
ProteinModelPortaliQ5SHH5.
SMRiQ5SHH5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1755.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71578; BAD71578; BAD71578.
GeneIDi3169449.
KEGGittj:TTHA1755.
PATRICi23958473. VBITheThe93045_1726.

Phylogenomic databases

eggNOGiENOG4105C8Y. Bacteria.
COG4992. LUCA.
HOGENOMiHOG000020206.
KOiK05830.
OMAiFSEIRGM.
PhylomeDBiQ5SHH5.

Enzyme and pathway databases

UniPathwayiUPA00033; UER00038.
UPA00068; UER00109.

Miscellaneous databases

EvolutionaryTraceiQ5SHH5.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPiMF_01107. ArgD_aminotrans_3. 1 hit.
InterProiIPR004636. AcOrn/SuccOrn_fam.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00707. argD. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARGD_THET8
AccessioniPrimary (citable) accession number: Q5SHH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.