Q5SGY0 (ARGB_THET8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylglutamate/acetylaminoadipate kinase
EC=2.7.2.-
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate/N-acetyl-L-aminoadipate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	TTHA1950

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in both the arginine and lysine biosynthetic pathways By similarity. HAMAP MF_00082_B
Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082_B ATP + N-acetyl-L-aminoadipate = ADP + N-acetyl-L-aminoadipate 5-phosphate. HAMAP MF_00082_B
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082_B Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 2/5. HAMAP MF_00082_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00082_B.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW lysine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetylglutamate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	Acetylglutamate/acetylaminoadipate kinase HAMAP MF_00082_B		PRO_0000264779
Regions
	Region	36 – 37	2	Substrate binding By similarity
Sites
	Binding site	58	1	Substrate By similarity
	Binding site	147	1	Substrate By similarity
	Site	8	1	Transition state stabilizer By similarity
	Site	209	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5SGY0 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: EA132B4C431FF1FD	FASTA	249	26,225
	10 20 30 40 50 60 MSEALLVKVG GSLRGAEALL EELAAYPGPL VLVHGGGPEI GEWLKRLGYE SRFVGGLRVT 70 80 90 100 110 120 PPEQLEVVEM ALYLTGKRLA WGLSRRGRKA LALSGRDALC LRGRALPELG RVGEVVGVEV 130 140 150 160 170 180 GLLLDLLEKG YTPLLAPIAL DEEGPLNVNA DTAAGAVAGA LGWPAVFLTD VEGVYRNPKD 190 200 210 220 230 240 PKTRFPRLTP KEVEALKEEG VIQGGMIPKV EAALAALRAG APWAAVAKGE RGVLRRLLSG EGGTLFSPS

References

[1]

"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008226 Genomic DNA. Translation: BAD71773.1.
RefSeq	YP_145216.1. NC_006461.1.
3D structure databases
HSSP	HSSP built from PDB template 2BUF based on UniProtKB Q9HTN2.
ProteinModelPortal	Q5SGY0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5SGY0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3168085.
GenomeReviews	Gene locus TTHA1950 in contig AP008226_GR.
KEGG	ttj:TTHA1950.
NMPDR	fig|300852.3.peg.1696.
PATRIC	23958881. VBITheThe93045_1922.
Phylogenomic databases
eggNOG	COG0548.
HOGENOM	HBG497643.
OMA	GGCLVDD.
PhylomeDB	Q5SGY0.
ProtClustDB	CLSK445594.
Enzyme and pathway databases
BioCyc	TTHE300852:TTHA1950-MONOMER.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00930.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. ArgB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_THET8

Accession

Primary (citable) accession number: Q5SGY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	December 21, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program