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Protein

Aldehyde oxidase 2

Gene

Aox2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Oxidase with broad substrate specificity, oxidizing aromatic azaheterocycles, such as phthalazine, as well as aldehydes, such as benzaldehyde and retinal. Cannot use hypoxanthine as substrate.1 Publication

Catalytic activityi

An aldehyde + H2O + O2 = a carboxylate + H2O2.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 2 [2Fe-2S] clusters per subunit.By similarity
  • FADBy similarityNote: Binds 1 FAD per subunit.By similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi53 – 531Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi56 – 561Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi78 – 781Iron-sulfur 1 (2Fe-2S)By similarity
Binding sitei117 – 1171MolybdopterinBy similarity
Metal bindingi118 – 1181Iron-sulfur 2 (2Fe-2S)By similarity
Metal bindingi121 – 1211Iron-sulfur 2 (2Fe-2S)By similarity
Metal bindingi153 – 1531Iron-sulfur 2 (2Fe-2S)By similarity
Metal bindingi155 – 1551Iron-sulfur 2 (2Fe-2S)By similarity
Binding sitei356 – 3561FADBy similarity
Binding sitei360 – 3601FADBy similarity
Binding sitei369 – 3691FADBy similarity
Binding sitei413 – 4131FAD; via amide nitrogenBy similarity
Binding sitei812 – 8121Molybdopterin; via amide nitrogenBy similarity
Binding sitei1053 – 10531Molybdopterin; via amide nitrogenBy similarity
Binding sitei1209 – 12091MolybdopterinBy similarity
Active sitei1276 – 12761Proton acceptor; for azaheterocycle hydroxylase activityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi266 – 2738FADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BRENDAi1.2.3.1. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Aldehyde oxidase 2 (EC:1.2.3.1)
Alternative name(s):
Aldehyde oxidase homolog 3
Azaheterocycle hydroxylase 2 (EC:1.17.3.-)
Gene namesi
Name:Aox2
Synonyms:Aoh3, Aox3l1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:3529596. Aox2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13451345Aldehyde oxidase 2PRO_0000425245Add
BLAST

Proteomic databases

MaxQBiQ5SGK3.
PaxDbiQ5SGK3.
PeptideAtlasiQ5SGK3.
PRIDEiQ5SGK3.

PTM databases

iPTMnetiQ5SGK3.
PhosphoSiteiQ5SGK3.

Expressioni

Tissue specificityi

Expressed in olfactory mucosa epithelium (at protein level). Detected in skin.2 Publications

Gene expression databases

BgeeiENSMUSG00000079554.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000110006.

Structurei

3D structure databases

ProteinModelPortaliQ5SGK3.
SMRiQ5SGK3. Positions 8-168.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 96882Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST
Domaini238 – 423186FAD-binding PCMH-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the xanthine dehydrogenase family.Curated
Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0430. Eukaryota.
COG4630. LUCA.
COG4631. LUCA.
GeneTreeiENSGT00390000003772.
HOGENOMiHOG000191197.
HOVERGENiHBG004182.
InParanoidiQ5SGK3.
KOiK00157.
OMAiNSFLCPE.
OrthoDBiEOG091G01AW.
PhylomeDBiQ5SGK3.
TreeFamiTF353036.

Family and domain databases

CDDicd00207. fer2. 1 hit.
Gene3Di1.10.150.120. 1 hit.
3.10.20.30. 1 hit.
3.30.365.10. 6 hits.
3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.1170.50. 1 hit.
InterProiIPR002888. 2Fe-2S-bd.
IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR000674. Ald_Oxase/Xan_DH_a/b.
IPR016208. Ald_Oxase/xanthine_DH.
IPR014313. Aldehyde_oxidase.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
IPR012675. Beta-grasp_dom.
IPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
[Graphical view]
PfamiPF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
PF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
PF00111. Fer2. 1 hit.
PF01799. Fer2_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000127. Xanthine_DH. 1 hit.
SMARTiSM01008. Ald_Xan_dh_C. 1 hit.
SM01092. CO_deh_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF47741. SSF47741. 1 hit.
SSF54292. SSF54292. 1 hit.
SSF54665. SSF54665. 1 hit.
SSF55447. SSF55447. 1 hit.
SSF56003. SSF56003. 1 hit.
SSF56176. SSF56176. 1 hit.
TIGRFAMsiTIGR02969. mam_aldehyde_ox. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS51387. FAD_PCMH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SGK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPCPAQISDD LEFFVNGRKV TEKNVDPEVT LLAFLRKNLC LTGTKDACGT
60 70 80 90 100
GGCGACTVMV SQHDPVCKKT RHFSVMACLV PLCSLHGAAV TTVEGVGSIK
110 120 130 140 150
TRLHPVQERI AKSHGTQCGF CTPGMVMSIY TLLRNHPQPS EEQLMEALGG
160 170 180 190 200
NLCRCTGYRP ILESGRTFCM EPDGCPQKGT GQCCLDQKES DSSGSKSDIC
210 220 230 240 250
TKLFVKDEFQ PLDPTQELIF PPELLRMAEN PEKQTLTFYG ERITWIAPGT
260 270 280 290 300
LQELLVLKAK YPEAPLISGN TALGPAMKSQ GHFYPVLLSP ARIPDLRMVT
310 320 330 340 350
KTSGGLTIGA CCSLAQVKDI LAESISELPQ EKTQTYRALL KHLRSLAGQQ
360 370 380 390 400
IRNMASLGGH VISRHCYSDL NPILSVGNTT LNLLSEEGPR QIPLSGHFLA
410 420 430 440 450
GLASADLKPE EILGSVYIPH SQKREFVSAF RQAQCHQNAL PDVNAGMRVL
460 470 480 490 500
FREGTDVIEE LSIAYGGVGP TTVSAQRSCQ QLLGRRWNAL MLDEACRLLL
510 520 530 540 550
DEVSLPGSAL GGKVEFRRTL IVSLFFKFYL EVLQELKADQ KLPPESTDSQ
560 570 580 590 600
RYPEIADRFL SSLGDFQVTL PRGVQTYQRV DSHQPLQDPV GRPIMHLSGL
610 620 630 640 650
KHATGEAVFC DDIPRVDKEL FMALVTSTRA HARIISIDSS EVLDLPGVVD
660 670 680 690 700
VITAEDIPGN NGEEDDKLLA VDKVLCVGQV ICAVVAETDV QAKRATEKIK
710 720 730 740 750
ITYEDLKPVI FTIEDAIKHN SFLCPEKKLE QGNIEEAFEN VDQVAEGTVH
760 770 780 790 800
VGGQEHFYME TQRVLVIPKT EDKELDMYVS TQDPAHVQKT VSSTLNIPIS
810 820 830 840 850
RITCHVKRVG GGFGGKVGRP AVFGAIAAVG AVKTGHPIRL VLDREDDMLI
860 870 880 890 900
TGGRHPLFAK YKVGFMNSGR IKALDIECYI NGGCTLDDSE LVTEFLVLKL
910 920 930 940 950
ENAYKIRNLR LRGRACMTNL PSNTAFRGFG FPQGALVTES CITAVAAKCG
960 970 980 990 1000
LPPEKIREKN MYKTVDKTIY KQAFNPDPLI RCWNECLDKS SFHIRRTRVD
1010 1020 1030 1040 1050
EFNKKSYWKK RGIAIVPMKF SVGFAATSYH QAAALVHIYT DGSVLVAHGG
1060 1070 1080 1090 1100
NELGQGIHTK MLQVASRELK IPLSYLHICE TSTTTVPNTI ATAASVGADV
1110 1120 1130 1140 1150
NGRAVQNACQ ILLKRLEPVI KKNPEGTWRD WIEAAFEKRI SLSATGYFRG
1160 1170 1180 1190 1200
YKAFMDWEKG EGDPFPYYVY GAACSEVEID CLTGAHKKIR TDIVMDACCS
1210 1220 1230 1240 1250
LNPAIDIGQI EGAFIQGMGL YTTEELLYSP EGVLYSRSPD KYKIPTVTDV
1260 1270 1280 1290 1300
PEQFNVSLLP SSQTPLTLYS SKGLGESGMF LGSSVFFAIV DAVAAARRQR
1310 1320 1330 1340
DIAEDFTVKS PATPEWVRMA CADRFTDMIP RDDPKTFKPW SIPIA
Length:1,345
Mass (Da):147,913
Last modified:January 4, 2005 - v2
Checksum:i6D3FA441A3F6F92C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti202 – 2021K → E in AAI50827 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY187018 mRNA. Translation: AAO38750.2.
AY665589 mRNA. Translation: AAV68256.1.
AC121091 Genomic DNA. No translation available.
AC163498 Genomic DNA. No translation available.
BC150826 mRNA. Translation: AAI50827.1.
CCDSiCCDS35576.1.
RefSeqiNP_001008419.1. NM_001008419.2.
UniGeneiMm.414292.

Genome annotation databases

EnsembliENSMUST00000114366; ENSMUSP00000110006; ENSMUSG00000079554.
GeneIDi213043.
KEGGimmu:213043.
UCSCiuc007bbp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY187018 mRNA. Translation: AAO38750.2.
AY665589 mRNA. Translation: AAV68256.1.
AC121091 Genomic DNA. No translation available.
AC163498 Genomic DNA. No translation available.
BC150826 mRNA. Translation: AAI50827.1.
CCDSiCCDS35576.1.
RefSeqiNP_001008419.1. NM_001008419.2.
UniGeneiMm.414292.

3D structure databases

ProteinModelPortaliQ5SGK3.
SMRiQ5SGK3. Positions 8-168.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000110006.

PTM databases

iPTMnetiQ5SGK3.
PhosphoSiteiQ5SGK3.

Proteomic databases

MaxQBiQ5SGK3.
PaxDbiQ5SGK3.
PeptideAtlasiQ5SGK3.
PRIDEiQ5SGK3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000114366; ENSMUSP00000110006; ENSMUSG00000079554.
GeneIDi213043.
KEGGimmu:213043.
UCSCiuc007bbp.1. mouse.

Organism-specific databases

CTDi213043.
MGIiMGI:3529596. Aox2.

Phylogenomic databases

eggNOGiKOG0430. Eukaryota.
COG4630. LUCA.
COG4631. LUCA.
GeneTreeiENSGT00390000003772.
HOGENOMiHOG000191197.
HOVERGENiHBG004182.
InParanoidiQ5SGK3.
KOiK00157.
OMAiNSFLCPE.
OrthoDBiEOG091G01AW.
PhylomeDBiQ5SGK3.
TreeFamiTF353036.

Enzyme and pathway databases

BRENDAi1.2.3.1. 3474.

Miscellaneous databases

PROiQ5SGK3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000079554.

Family and domain databases

CDDicd00207. fer2. 1 hit.
Gene3Di1.10.150.120. 1 hit.
3.10.20.30. 1 hit.
3.30.365.10. 6 hits.
3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.1170.50. 1 hit.
InterProiIPR002888. 2Fe-2S-bd.
IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR000674. Ald_Oxase/Xan_DH_a/b.
IPR016208. Ald_Oxase/xanthine_DH.
IPR014313. Aldehyde_oxidase.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
IPR012675. Beta-grasp_dom.
IPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
[Graphical view]
PfamiPF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
PF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
PF00111. Fer2. 1 hit.
PF01799. Fer2_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000127. Xanthine_DH. 1 hit.
SMARTiSM01008. Ald_Xan_dh_C. 1 hit.
SM01092. CO_deh_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF47741. SSF47741. 1 hit.
SSF54292. SSF54292. 1 hit.
SSF54665. SSF54665. 1 hit.
SSF55447. SSF55447. 1 hit.
SSF56003. SSF56003. 1 hit.
SSF56176. SSF56176. 1 hit.
TIGRFAMsiTIGR02969. mam_aldehyde_ox. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS51387. FAD_PCMH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAOXB_MOUSE
AccessioniPrimary (citable) accession number: Q5SGK3
Secondary accession number(s): B9EKC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: January 4, 2005
Last modified: September 7, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

AOX genes evolved from a xanthine oxidoreductase ancestral precursor via a series of gene duplication and suppression/deletion events. Different animal species contain a different complement of AOX genes encoding an equivalent number of AOX isoenzymes. In mammals, the two extremes are represented by certain rodents such as mice and rats, which are endowed with 4 AOX genes, and by humans, whose genome is characterized by a single active gene (PubMed:23263164).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.