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Q5SGD2 (PPM1L_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 1L

EC=3.1.3.16
Alternative name(s):
Protein phosphatase 1-like
Protein phosphatase 2C isoform epsilon
Short name=PP2C-epsilon
Gene names
Name:PPM1L
Synonyms:PP2CE
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a suppressor of the SAPK signaling pathways by associating with and dephosphorylating MAP3K7/TAK1 and MAP3K5, and by attenuating the association between MAP3K7/TAK1 and MAP2K4 or MAP2K6. Ref.4

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Subunit structure

Interacts with MAP3K7/TAK1 By similarity. Interacts with MAP3K5. Ref.4

Subcellular location

Membrane; Single-pass type I membrane protein Potential.

Tissue specificity

Ubiquitous. Highly expressed in heart, placenta, lung, liver, kidney and pancreas. Ref.1

Sequence similarities

Belongs to the PP2C family.

Contains 1 PP2C-like domain.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q5SGD2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5SGD2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-179: Missing.
Isoform 3 (identifier: Q5SGD2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-127: Missing.
     128-133: DGHGGE → MPAFST
Isoform 4 (identifier: Q5SGD2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     192-194: GTT → VTF
     195-360: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360Protein phosphatase 1L
PRO_0000057754

Regions

Topological domain1 – 2525Extracellular Potential
Transmembrane26 – 4217Helical; Potential
Topological domain43 – 360318Cytoplasmic Potential
Domain91 – 344254PP2C-like

Sites

Metal binding1281Manganese 1 By similarity
Metal binding1281Manganese 2 By similarity
Metal binding1291Manganese 1; via carbonyl oxygen By similarity
Metal binding3021Manganese 2 By similarity
Metal binding3421Manganese 2 By similarity

Natural variations

Alternative sequence1 – 179179Missing in isoform 2.
VSP_016927
Alternative sequence1 – 127127Missing in isoform 3.
VSP_037552
Alternative sequence128 – 1336DGHGGE → MPAFST in isoform 3.
VSP_037553
Alternative sequence192 – 1943GTT → VTF in isoform 4.
VSP_037554
Alternative sequence195 – 360166Missing in isoform 4.
VSP_037555
Natural variant2621A → S.
Corresponds to variant rs13326359 [ dbSNP | Ensembl ].
VAR_050622

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: 08212E7BA32AFE2B

FASTA36041,053
        10         20         30         40         50         60 
MIEDTMTLLS LLGRIMRYFL LRPETLFLLC ISLALWSYFF HTDEVKTIVK SSRDAVKMVK 

        70         80         90        100        110        120 
GKVAEIMQND RLGGLDVLEA EFSKTWEFKN HNVAVYSIQG RRDHMEDRFE VLTDLANKTH 

       130        140        150        160        170        180 
PSIFGIFDGH GGETAAEYVK SRLPEALKQH LQDYEKDKEN SVLSYQTILE QQILSIDREM 

       190        200        210        220        230        240 
LEKLTVSYDE AGTTCLIALL SDKDLTVANV GDSRGVLCDK DGNAIPLSHD HKPYQLKERK 

       250        260        270        280        290        300 
RIKRAGGFIS FNGSWRVQGI LAMSRSLGDY PLKNLNVVIP DPDILTFDLD KLQPEFMILA 

       310        320        330        340        350        360 
SDGLWDAFSN EEAVRFIKER LDEPHFGAKS IVLQSFYRGC PDNITVMVVK FRNSSKTEEQ 

« Hide

Isoform 2 [UniParc].

Checksum: 74F07366F3C3FB4B
Show »

FASTA18120,324
Isoform 3 [UniParc].

Checksum: 5522C3B41C506168
Show »

FASTA23326,363
Isoform 4 [UniParc].

Checksum: BD4465CB810BCBB9
Show »

FASTA19422,475

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of a novel human protein phosphatase 2C cDNA (PP2C epsilon)."
Jin F., Ji C., Liu L., Dai J., Gu S., Sun X., Xie Y., Mao Y.
Mol. Biol. Rep. 31:197-202(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Tissue: Fetal brain.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4).
Tissue: Brain.
[4]"Regulation of apoptosis signal-regulating kinase 1 by protein phosphatase 2Cepsilon."
Saito J., Toriumi S., Awano K., Ichijo H., Sasaki K., Kobayashi T., Tamura S.
Biochem. J. 405:591-596(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MAP3K5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY337264 mRNA. Translation: AAR00269.1.
AK055115 mRNA. Translation: BAB70856.1.
BC104885 mRNA. Translation: AAI04886.1.
BC104887 mRNA. Translation: AAI04888.1.
BC110801 mRNA. No translation available.
CCDSCCDS33886.1. [Q5SGD2-1]
RefSeqNP_640338.2. NM_139245.2. [Q5SGD2-1]
UniGeneHs.389027.

3D structure databases

ProteinModelPortalQ5SGD2.
SMRQ5SGD2. Positions 93-352.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid127400. 2 interactions.
STRING9606.ENSP00000417659.

PTM databases

PhosphoSiteQ5SGD2.

Polymorphism databases

DMDM74743437.

Proteomic databases

MaxQBQ5SGD2.
PaxDbQ5SGD2.
PRIDEQ5SGD2.

Protocols and materials databases

DNASU151742.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000295839; ENSP00000295839; ENSG00000163590. [Q5SGD2-3]
ENST00000464260; ENSP00000420746; ENSG00000163590. [Q5SGD2-2]
ENST00000497343; ENSP00000420354; ENSG00000163590. [Q5SGD2-4]
ENST00000498165; ENSP00000417659; ENSG00000163590. [Q5SGD2-1]
GeneID151742.
KEGGhsa:151742.
UCSCuc003fdr.3. human. [Q5SGD2-1]
uc003fdt.3. human. [Q5SGD2-3]

Organism-specific databases

CTD151742.
GeneCardsGC03P160473.
HGNCHGNC:16381. PPM1L.
HPAHPA019891.
HPA019953.
MIM611931. gene.
neXtProtNX_Q5SGD2.
PharmGKBPA134871016.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0631.
HOGENOMHOG000233896.
HOVERGENHBG079483.
InParanoidQ5SGD2.
KOK17506.
OMATERIVAC.
OrthoDBEOG74J98W.
PhylomeDBQ5SGD2.
TreeFamTF332888.

Enzyme and pathway databases

BRENDA3.1.3.16. 2681.
ReactomeREACT_111217. Metabolism.

Gene expression databases

BgeeQ5SGD2.
CleanExHS_PPM1L.
GenevestigatorQ5SGD2.

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 1 hit.
PROSITEPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi151742.
NextBio86779.
PROQ5SGD2.
SOURCESearch...

Entry information

Entry namePPM1L_HUMAN
AccessionPrimary (citable) accession number: Q5SGD2
Secondary accession number(s): Q2M3J2, Q96NM7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: December 21, 2004
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM