Q5SGD2 (PPM1L_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 1L
EC=3.1.3.16

Alternative name(s):
Protein phosphatase 1-like
Protein phosphatase 2C isoform epsilon
Short name=PP2C-epsilon

Gene names

Name:	PPM1L
Synonyms:	PP2CE

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a suppressor of the SAPK signaling pathways by associating with and dephosphorylating MAP3K7/TAK1 and MAP3K5, and by attenuating the association between MAP3K7/TAK1 and MAP2K4 or MAP2K6. Ref.4
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subunit structure	Interacts with MAP3K7/TAK1 By similarity. Interacts with MAP3K5. Ref.4
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Tissue specificity	Ubiquitous. Highly expressed in heart, placenta, lung, liver, kidney and pancreas. Ref.1
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	MAPK cascade Inferred from electronic annotation. Source: Compara phospholipid metabolic process Traceable author statement. Source: Reactome protein dephosphorylation Inferred from electronic annotation. Source: InterPro small molecule metabolic process Traceable author statement. Source: Reactome sphingolipid biosynthetic process Traceable author statement. Source: Reactome transmembrane receptor protein serine/threonine kinase signaling pathway Inferred from electronic annotation. Source: Compara
Cellular_component	endoplasmic reticulum membrane Traceable author statement. Source: Reactome integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Traceable author statement. Source: Reactome
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5SGD2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5SGD2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-179: Missing.

Isoform 3 (identifier: Q5SGD2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-127: Missing.
     128-133: DGHGGE → MPAFST

Isoform 4 (identifier: Q5SGD2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     192-194: GTT → VTF
     195-360: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Protein phosphatase 1L		PRO_0000057754
Regions
	Topological domain	1 – 25	25	Extracellular Potential
	Transmembrane	26 – 42	17	Helical; Potential
	Topological domain	43 – 360	318	Cytoplasmic Potential
	Domain	91 – 344	254	PP2C-like
Sites
	Metal binding	128	1	Manganese 1 By similarity
	Metal binding	128	1	Manganese 2 By similarity
	Metal binding	129	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	302	1	Manganese 2 By similarity
	Metal binding	342	1	Manganese 2 By similarity
Natural variations
	Alternative sequence	1 – 179	179	Missing in isoform 2.		VSP_016927
	Alternative sequence	1 – 127	127	Missing in isoform 3.		VSP_037552
	Alternative sequence	128 – 133	6	DGHGGE → MPAFST in isoform 3.		VSP_037553
	Alternative sequence	192 – 194	3	GTT → VTF in isoform 4.		VSP_037554
	Alternative sequence	195 – 360	166	Missing in isoform 4.		VSP_037555
	Natural variant	262	1	A → S. Corresponds to variant rs13326359 [ dbSNP | Ensembl ].		VAR_050622

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 08212E7BA32AFE2B	FASTA	360	41,053
	10 20 30 40 50 60 MIEDTMTLLS LLGRIMRYFL LRPETLFLLC ISLALWSYFF HTDEVKTIVK SSRDAVKMVK 70 80 90 100 110 120 GKVAEIMQND RLGGLDVLEA EFSKTWEFKN HNVAVYSIQG RRDHMEDRFE VLTDLANKTH 130 140 150 160 170 180 PSIFGIFDGH GGETAAEYVK SRLPEALKQH LQDYEKDKEN SVLSYQTILE QQILSIDREM 190 200 210 220 230 240 LEKLTVSYDE AGTTCLIALL SDKDLTVANV GDSRGVLCDK DGNAIPLSHD HKPYQLKERK 250 260 270 280 290 300 RIKRAGGFIS FNGSWRVQGI LAMSRSLGDY PLKNLNVVIP DPDILTFDLD KLQPEFMILA 310 320 330 340 350 360 SDGLWDAFSN EEAVRFIKER LDEPHFGAKS IVLQSFYRGC PDNITVMVVK FRNSSKTEEQ
	Isoform 2 [UniParc]. Checksum: 74F07366F3C3FB4B	FASTA	181	20,324
	10 20 30 40 50 60 MLEKLTVSYD EAGTTCLIAL LSDKDLTVAN VGDSRGVLCD KDGNAIPLSH DHKPYQLKER 70 80 90 100 110 120 KRIKRAGGFI SFNGSWRVQG ILAMSRSLGD YPLKNLNVVI PDPDILTFDL DKLQPEFMIL 130 140 150 160 170 180 ASDGLWDAFS NEEAVRFIKE RLDEPHFGAK SIVLQSFYRG CPDNITVMVV KFRNSSKTEE Q
	Isoform 3 [UniParc]. Checksum: 5522C3B41C506168	FASTA	233	26,363
	10 20 30 40 50 60 MPAFSTTAAE YVKSRLPEAL KQHLQDYEKD KENSVLSYQT ILEQQILSID REMLEKLTVS 70 80 90 100 110 120 YDEAGTTCLI ALLSDKDLTV ANVGDSRGVL CDKDGNAIPL SHDHKPYQLK ERKRIKRAGG 130 140 150 160 170 180 FISFNGSWRV QGILAMSRSL GDYPLKNLNV VIPDPDILTF DLDKLQPEFM ILASDGLWDA 190 200 210 220 230 FSNEEAVRFI KERLDEPHFG AKSIVLQSFY RGCPDNITVM VVKFRNSSKT EEQ
	Isoform 4 [UniParc]. Checksum: BD4465CB810BCBB9	FASTA	194	22,475
	10 20 30 40 50 60 MIEDTMTLLS LLGRIMRYFL LRPETLFLLC ISLALWSYFF HTDEVKTIVK SSRDAVKMVK 70 80 90 100 110 120 GKVAEIMQND RLGGLDVLEA EFSKTWEFKN HNVAVYSIQG RRDHMEDRFE VLTDLANKTH 130 140 150 160 170 180 PSIFGIFDGH GGETAAEYVK SRLPEALKQH LQDYEKDKEN SVLSYQTILE QQILSIDREM 190 LEKLTVSYDE AVTF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a novel human protein phosphatase 2C cDNA (PP2C epsilon)." Jin F., Ji C., Liu L., Dai J., Gu S., Sun X., Xie Y., Mao Y. Mol. Biol. Rep. 31:197-202(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Fetal brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4). Tissue: Brain.
[4]	"Regulation of apoptosis signal-regulating kinase 1 by protein phosphatase 2Cepsilon." Saito J., Toriumi S., Awano K., Ichijo H., Sasaki K., Kobayashi T., Tamura S. Biochem. J. 405:591-596(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MAP3K5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY337264 mRNA. Translation: AAR00269.1. AK055115 mRNA. Translation: BAB70856.1. BC104885 mRNA. Translation: AAI04886.1. BC104887 mRNA. Translation: AAI04888.1. BC110801 mRNA. No translation available.
IPI	IPI00186145. IPI00657835. IPI00938040. IPI00938269.
RefSeq	NP_640338.2. NM_139245.2.
UniGene	Hs.389027.
3D structure databases
ProteinModelPortal	Q5SGD2.
SMR	Q5SGD2. Positions 97-352.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000417659.
PTM databases
PhosphoSite	Q5SGD2.
Polymorphism databases
DMDM	74743437.
Proteomic databases
PaxDb	Q5SGD2.
PRIDE	Q5SGD2.
Protocols and materials databases
DNASU	151742.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000295839; ENSP00000295839; ENSG00000163590. ENST00000464260; ENSP00000420746; ENSG00000163590. ENST00000497343; ENSP00000420354; ENSG00000163590. ENST00000498165; ENSP00000417659; ENSG00000163590.
GeneID	151742.
KEGG	hsa:151742.
UCSC	uc003fdr.3. human. uc003fdt.3. human.
Organism-specific databases
CTD	151742.
GeneCards	GC03P160473.
HGNC	HGNC:16381. PPM1L.
HPA	HPA019891. HPA019953.
MIM	611931. gene.
neXtProt	NX_Q5SGD2.
PharmGKB	PA134871016.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233896.
HOVERGEN	HBG079483.
InParanoid	Q5SGD2.
OMA	TERIVAC.
OrthoDB	EOG466VM4.
Enzyme and pathway databases
BRENDA	3.1.3.16. 2681.
Reactome	REACT_111217. Metabolism.
Gene expression databases
Bgee	Q5SGD2.
CleanEx	HS_PPM1L.
Genevestigator	Q5SGD2.
GermOnline	ENSG00000163590. Homo sapiens.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	151742.
NextBio	86779.
SOURCE	Search...

Entry information

Entry name

PPM1L_HUMAN

Accession

Primary (citable) accession number: Q5SGD2
Secondary accession number(s): Q2M3J2, Q96NM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	December 21, 2004
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.