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Protein

Insulin-like growth factor 2 mRNA-binding protein 2

Gene

Igf2bp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation (By similarity). Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. Binds to beta-actin/ACTB and MYC transcripts (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA transport, Translation regulation, Transport

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like growth factor 2 mRNA-binding protein 2
Short name:
IGF2 mRNA-binding protein 2
Short name:
IMP-2
Alternative name(s):
IGF-II mRNA-binding protein 2
VICKZ family member 2
Gene namesi
Name:Igf2bp2
Synonyms:Imp2, Vickz2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1890358. Igf2bp2.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa. In response to cellular stress, such as oxidative stress, recruited to stress granules (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002444971 – 592Insulin-like growth factor 2 mRNA-binding protein 2Add BLAST592

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei11PhosphoserineBy similarity1
Modified residuei162PhosphoserineCombined sources1
Modified residuei164PhosphoserineCombined sources1
Modified residuei543PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5SF07.
PeptideAtlasiQ5SF07.
PRIDEiQ5SF07.

2D gel databases

REPRODUCTION-2DPAGEQ5SF07.

PTM databases

iPTMnetiQ5SF07.
PhosphoSitePlusiQ5SF07.

Expressioni

Tissue specificityi

Expressed in oocytes, granulosa cells of small and growing follicles and Leydig cells of the testis (at protein level). Expressed in testis and ovary.1 Publication

Developmental stagei

Expressed in zygotes and blastocysts (at protein level). Expressed in gonads at 12.5, 14.5 and 16.5 dpc (at protein level). Expressed during fetal development at 12.5, 14.5 and 17.5 dpc and declining towards birth.2 Publications

Gene expression databases

BgeeiENSMUSG00000033581.
ExpressionAtlasiQ5SF07. baseline and differential.
GenevisibleiQ5SF07. MM.

Interactioni

Subunit structurei

Can form homooligomers and heterooligomers with IGF2BP1 and IGF2BP3 in an RNA-dependent manner. Interacts with HNRPD and IGF2BP1. Interacts with ELAVL1, DHX9 and HNRNPU (By similarity).By similarity

Protein-protein interaction databases

BioGridi235510. 2 interactors.
IntActiQ5SF07. 1 interactor.
STRINGi10090.ENSMUSP00000097629.

Structurei

3D structure databases

ProteinModelPortaliQ5SF07.
SMRiQ5SF07.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 76RRM 1PROSITE-ProRule annotationAdd BLAST74
Domaini82 – 157RRM 2PROSITE-ProRule annotationAdd BLAST76
Domaini186 – 251KH 1PROSITE-ProRule annotationAdd BLAST66
Domaini267 – 334KH 2PROSITE-ProRule annotationAdd BLAST68
Domaini420 – 485KH 3PROSITE-ProRule annotationAdd BLAST66
Domaini502 – 568KH 4PROSITE-ProRule annotationAdd BLAST67

Domaini

Domain KH3 and KH4 are the major RNA-binding modules, although KH1 and KH2 may also contribute. The contribution to RNA-binding of individual KH domains may be target-specific. KH1 and KH2, and possibly KH3 and KH4, promote the formation of higher ordered protein-RNA complexes, which may be essential for IGF2BP1 cytoplasmic retention. KH domains are required for RNA-dependent homo- and heterooligomerization and for localization to stress granules (By similarity).By similarity

Sequence similaritiesi

Belongs to the RRM IMP/VICKZ family.Curated
Contains 4 KH domains.PROSITE-ProRule annotation
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2193. Eukaryota.
ENOG410ZKB4. LUCA.
GeneTreeiENSGT00530000063171.
HOGENOMiHOG000000675.
HOVERGENiHBG052725.
InParanoidiQ5SF07.
KOiK17392.
OMAiPTINARY.
OrthoDBiEOG091G17T1.
PhylomeDBiQ5SF07.
TreeFamiTF320229.

Family and domain databases

Gene3Di3.30.1370.10. 4 hits.
3.30.70.330. 2 hits.
InterProiIPR028743. IGF2BP2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR10288:SF93. PTHR10288:SF93. 1 hit.
PfamiPF00013. KH_1. 4 hits.
PF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00322. KH. 4 hits.
SM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 4 hits.
SSF54928. SSF54928. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 4 hits.
PS50102. RRM. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5SF07-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMNKLYIGNL SPAVTADDLR QLFGDRKLPL AGQVLLKSGY AFVDYPDQNW
60 70 80 90 100
AIRAIETLSG KVELHGKIME VDYSVSKKLR SRRIQIRNIP PHLQWEVLDG
110 120 130 140 150
LLAEYGTVEN VEQVNTDTET AVVNVTYMTR EEAKLAIEKL SGHQFEDYSF
160 170 180 190 200
KISYIPDEEV SSPSPPHRAR EQGHGPGSSS QARQIDFPLR ILVPTQFVGA
210 220 230 240 250
IIGKEGLTIK NITKQTQSRV DIHRKENSGA AEKPVTIHAT PEGTSEACRM
260 270 280 290 300
ILEIMQKEAD ETKLAEEVPL KILAHNGFVG RLIGKEGRNL KKIEHETGTK
310 320 330 340 350
ITISSLQDLS IYNPERTITV RGTIEACANA EIEIMKKLRE AFENDMLAVN
360 370 380 390 400
QQANLIPGLN LSALGIFSTG LSVLPPPAGP RGVPPSPPYH PFATHSGYFS
410 420 430 440 450
SLYPHHHFGP FPHHHSYPEQ ETVSLFIPTQ AVGAIIGKKG AHIKQLARFA
460 470 480 490 500
GASIKIAPAE GPDVSERMVI ITGPPEAQFK AQGRIFGKLK EENFFNPKEE
510 520 530 540 550
VKLEAHIRVP SSTAGRVIGK GGKTVNELQN LTSAEVIVPR DQTPDENEEV
560 570 580 590
IVRIIGHFFA SQTAQRKIRE IVQQVKQQEQ RYPQGVAPQR SK
Length:592
Mass (Da):65,584
Last modified:December 21, 2004 - v1
Checksum:i9D0E420758F5D1BE
GO
Isoform 2 (identifier: Q5SF07-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: MMNKLYIGNL...EVDYSVSKKL → MHPSQWPRYGAAGKPFS

Show »
Length:530
Mass (Da):58,659
Checksum:iFB5F89AC81D0835D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti568I → V in BAE41861 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0195761 – 79MMNKL…VSKKL → MHPSQWPRYGAAGKPFS in isoform 2. 1 PublicationAdd BLAST79

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY531659 mRNA. Translation: AAT01428.1.
AK170531 mRNA. Translation: BAE41861.1.
CT009567 Genomic DNA. Translation: CAO78169.1.
CCDSiCCDS49797.1. [Q5SF07-1]
RefSeqiNP_898850.2. NM_183029.2. [Q5SF07-1]
UniGeneiMm.489442.

Genome annotation databases

EnsembliENSMUST00000100052; ENSMUSP00000097629; ENSMUSG00000033581. [Q5SF07-1]
GeneIDi319765.
KEGGimmu:319765.
UCSCiuc007yrz.1. mouse. [Q5SF07-2]
uc007ysa.1. mouse. [Q5SF07-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY531659 mRNA. Translation: AAT01428.1.
AK170531 mRNA. Translation: BAE41861.1.
CT009567 Genomic DNA. Translation: CAO78169.1.
CCDSiCCDS49797.1. [Q5SF07-1]
RefSeqiNP_898850.2. NM_183029.2. [Q5SF07-1]
UniGeneiMm.489442.

3D structure databases

ProteinModelPortaliQ5SF07.
SMRiQ5SF07.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi235510. 2 interactors.
IntActiQ5SF07. 1 interactor.
STRINGi10090.ENSMUSP00000097629.

PTM databases

iPTMnetiQ5SF07.
PhosphoSitePlusiQ5SF07.

2D gel databases

REPRODUCTION-2DPAGEQ5SF07.

Proteomic databases

PaxDbiQ5SF07.
PeptideAtlasiQ5SF07.
PRIDEiQ5SF07.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000100052; ENSMUSP00000097629; ENSMUSG00000033581. [Q5SF07-1]
GeneIDi319765.
KEGGimmu:319765.
UCSCiuc007yrz.1. mouse. [Q5SF07-2]
uc007ysa.1. mouse. [Q5SF07-1]

Organism-specific databases

CTDi10644.
MGIiMGI:1890358. Igf2bp2.

Phylogenomic databases

eggNOGiKOG2193. Eukaryota.
ENOG410ZKB4. LUCA.
GeneTreeiENSGT00530000063171.
HOGENOMiHOG000000675.
HOVERGENiHBG052725.
InParanoidiQ5SF07.
KOiK17392.
OMAiPTINARY.
OrthoDBiEOG091G17T1.
PhylomeDBiQ5SF07.
TreeFamiTF320229.

Miscellaneous databases

ChiTaRSiIgf2bp2. mouse.
PROiQ5SF07.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033581.
ExpressionAtlasiQ5SF07. baseline and differential.
GenevisibleiQ5SF07. MM.

Family and domain databases

Gene3Di3.30.1370.10. 4 hits.
3.30.70.330. 2 hits.
InterProiIPR028743. IGF2BP2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR10288:SF93. PTHR10288:SF93. 1 hit.
PfamiPF00013. KH_1. 4 hits.
PF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00322. KH. 4 hits.
SM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 4 hits.
SSF54928. SSF54928. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 4 hits.
PS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF2B2_MOUSE
AccessioniPrimary (citable) accession number: Q5SF07
Secondary accession number(s): A6X8Z4, Q3TCU4, Q7TQF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.