Q5SDA5 (GUC2F_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Retinal guanylyl cyclase 2
EC=4.6.1.2

Gene names

Name:

Gucy2f

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1108 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probably plays a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction By similarity.
Catalytic activity	GTP = 3',5'-cyclic GMP + diphosphate.
Enzyme regulation	Activated by GCAP-1; inhibited by calcium By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein By similarity.
Domain	The protein kinase domain is predicted to be catalytically inactive.
Post-translational modification	There are 9 conserved cysteine residues in sensory guanylate cyclases, 6 in the extracellular domain, which may be involved in intra- or interchain disulfide bonds By similarity.
Sequence similarities	Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 1 guanylate cyclase domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Sensory transduction Vision cGMP biosynthesis
Cellular component	Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	GTP-binding Nucleotide-binding
Molecular function	Lyase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: InterPro GTP binding Inferred from electronic annotation. Source: UniProtKB-KW guanylate cyclase activity Inferred from electronic annotation. Source: EC protein kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 50	50	Potential
	Chain	51 – 1108	1058	Retinal guanylyl cyclase 2		PRO_0000280445
Regions
	Topological domain	51 – 469	419	Extracellular Potential
	Transmembrane	470 – 490	21	Helical; Potential
	Topological domain	491 – 1108	618	Cytoplasmic Potential
	Domain	532 – 812	281	Protein kinase
	Domain	884 – 1014	131	Guanylate cyclase
Amino acid modifications
	Disulfide bond	104 ↔ 132		By similarity
	Disulfide bond	452		Interchain By similarity
	Disulfide bond	460		Interchain By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5SDA5 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 8A1456FCB384FA90	FASTA	1,108	124,425
	10 20 30 40 50 60 MFLGPWPFSR LLSWFAISSR LSGQHGLPSS KFLRCLCLLA LLPLLRWGQA LPYKIGVIGP 70 80 90 100 110 120 WTCDPFFSKA LPEVAAALAI ERISRDKTFD RSYSFEYVIL NEDCQTSKAL ASFISHQQMA 130 140 150 160 170 180 SGFVGPANPG FCEAASLLGT SWDKGIFSWA CVNHELDNKH SFPTFSRTLP SPIRVLVTVM 190 200 210 220 230 240 KYFQWAHAGV ISSDEDIWMH TANRVSSALR SQGLPVGVVL TSGRDSQSIQ KALQQIRQAD 250 260 270 280 290 300 RIRIIIMCMH SALIGGETQT HFLELAHDLK MTDGTYVFVP YDVLLYSLPY KHSPYQVLRN 310 320 330 340 350 360 NPKLREAYDA VLTITVESHE KTFYEAYAEA AARGEIPEKP DSNQVSPLFG TIYNSIYFIA 370 380 390 400 410 420 QAMNNAMKKN GRASAASLVQ HSRNMQFYGF NQLIKTDSNG NGISEYVILD TNGKEWELRG 430 440 450 460 470 480 TYTVDMETEL LRFRGTPIHF PGGRPTSADA KCWFAERKIC QGGIDPALAM MVCFALLIAL 490 500 510 520 530 540 LSINGFAYFI RRRINKIQLI KGPNRILLTL EDVTFINPHF GSKRGSRASV SFQIISEVQS 550 560 570 580 590 600 GRSPRLSFSS GSLTPATYEN SNIAIYEGDW VWLKKFPPGD FGDIKSIKSS ASDVFEMMKD 610 620 630 640 650 660 LRHENVNPLL GFFYDSGMFA IVSEFCSRRS LEDILTNDDV KLDWMFKSSL LLDLIKGMKY 670 680 690 700 710 720 LHHREFIHGR LKSRNCVVDG RFVLKVTDYG FNDILEMLRL SEEEPSEEEL LWTAPELLRA 730 740 750 760 770 780 PGGIRLGSFA GDVYSFAIIM QEVMVRGAPF CMMDLPAKEI IDRLKMPPPV YRPVVSPEYA 790 800 810 820 830 840 PAECLQLMKQ CWAEASEQRP TFDEIFNQFK TFNKGKKTNI IDSMLRMLEQ YSSNLEDLIR 850 860 870 880 890 900 ERTEELEIEK QKTEKLLTQM LPLSVAESLK KGCTVEPEGF DLVTLYFSDI VGFTTISAMS 910 920 930 940 950 960 EPIEVVDLLN DLYTLFDAII GSHDVYKVET IGDAYMVASG LPKRNGSRHA AEIANMSLDI 970 980 990 1000 1010 1020 LSSVGTFKMR HMPEVPVRIR IGLHSGPVVA GVVGLTMPRY CLFGDTVNTA SRMESTGLPY 1030 1040 1050 1060 1070 1080 RIHVSLSTVT ILQTLSEGYE VELRGRTELK GKGTEETFWL VGKKGFTKPL PVPPPVGKDG 1090 1100 QVGHGLQPAE IAAFQRRKAE RQLVRNKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Application of functional genomic technologies in a mouse model of retinal degeneration." Shearstone J.R., Wang Y.E., Clement A., Allaire N.E., Yang C., Worley D.S., Carulli J.P., Perrin S. Genomics 85:309-321(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Retina.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-846. Strain: C57BL/6J. Tissue: Retina.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY651761 mRNA. Translation: AAV54098.1. AL671916, BX324191 Genomic DNA. Translation: CAM19986.1. BX324191, AL671916 Genomic DNA. Translation: CAM24899.1. BC115714 mRNA. Translation: AAI15715.1. AK044234 mRNA. Translation: BAC31833.1.
IPI	IPI00345822.
RefSeq	NP_001007577.1. NM_001007576.2.
UniGene	Mm.208511.
3D structure databases
HSSP	HSSP built from PDB template 1YK9 based on UniProtKB P0A4Y0.
ProteinModelPortal	Q5SDA5.
SMR	Q5SDA5. Positions 75-246, 555-837, 884-1060.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000044521.
PTM databases
PhosphoSite	Q5SDA5.
Proteomic databases
PRIDE	Q5SDA5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000042530; ENSMUSP00000044521; ENSMUSG00000042282.
GeneID	245650.
KEGG	mmu:245650.
UCSC	uc009ulq.1. mouse.
Organism-specific databases
CTD	2986.
MGI	MGI:105119. Gucy2f.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00560000076744.
HOGENOM	HOG000293307.
HOVERGEN	HBG098487.
InParanoid	Q5SDA5.
KO	K12321.
OrthoDB	EOG4XD3QC.
Gene expression databases
Bgee	Q5SDA5.
CleanEx	MM_GUCY2F.
Genevestigator	Q5SDA5.
Family and domain databases
Gene3D	3.30.70.1230. 1 hit.
InterPro	IPR001054. A/G_cyclase. IPR018297. A/G_cyclase_CS. IPR001828. ANF_lig-bd_rcpt. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. [Graphical view]
Pfam	PF01094. ANF_receptor. 1 hit. PF00211. Guanylate_cyc. 1 hit. PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
SMART	SM00044. CYCc. 1 hit. [Graphical view]
SUPFAM	SSF55073. A/G_cyclase. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS00452. GUANYLATE_CYCLASE_1. 1 hit. PS50125. GUANYLATE_CYCLASE_2. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	386884.
SOURCE	Search...

Entry information

Entry name

GUC2F_MOUSE

Accession

Primary (citable) accession number: Q5SDA5
Secondary accession number(s): Q8BLL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	December 21, 2004
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program