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Q5SDA5

- GUC2F_MOUSE

UniProt

Q5SDA5 - GUC2F_MOUSE

Protein

Retinal guanylyl cyclase 2

Gene

Gucy2f

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (21 Dec 2004)
      Previous versions | rss
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    Functioni

    Probably plays a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction By similarity.By similarity

    Catalytic activityi

    GTP = 3',5'-cyclic GMP + diphosphate.

    Enzyme regulationi

    Activated by GCAP-1; inhibited by calcium.By similarity

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. GTP binding Source: UniProtKB-KW
    3. guanylate cyclase activity Source: UniProtKB-EC
    4. protein kinase activity Source: InterPro

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. visual perception Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    cGMP biosynthesis, Sensory transduction, Vision

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_188266. Inactivation, recovery and regulation of the phototransduction cascade.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Retinal guanylyl cyclase 2 (EC:4.6.1.2)
    Gene namesi
    Name:Gucy2f
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:105119. Gucy2f.

    Subcellular locationi

    Membrane By similarity; Single-pass type I membrane protein By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 5050Sequence AnalysisAdd
    BLAST
    Chaini51 – 11081058Retinal guanylyl cyclase 2PRO_0000280445Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi104 ↔ 132By similarity
    Disulfide bondi452 – 452InterchainBy similarity
    Disulfide bondi460 – 460InterchainBy similarity

    Post-translational modificationi

    There are 9 conserved cysteine residues in sensory guanylate cyclases, 6 in the extracellular domain, which may be involved in intra- or interchain disulfide bonds.By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiQ5SDA5.

    PTM databases

    PhosphoSiteiQ5SDA5.

    Expressioni

    Gene expression databases

    BgeeiQ5SDA5.
    CleanExiMM_GUCY2F.
    GenevestigatoriQ5SDA5.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000044521.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5SDA5.
    SMRiQ5SDA5. Positions 75-246, 596-855, 884-1060.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini51 – 469419ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini491 – 1108618CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei470 – 49021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini532 – 812281Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini884 – 1014131Guanylate cyclasePROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The protein kinase domain is predicted to be catalytically inactive.

    Sequence similaritiesi

    Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
    Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00730000110520.
    HOGENOMiHOG000293307.
    HOVERGENiHBG098487.
    InParanoidiQ5SDA5.
    KOiK12321.
    OMAiNSIYFIA.
    OrthoDBiEOG7QVM1V.
    PhylomeDBiQ5SDA5.
    TreeFamiTF106338.

    Family and domain databases

    Gene3Di3.30.70.1230. 1 hit.
    InterProiIPR001054. A/G_cyclase.
    IPR018297. A/G_cyclase_CS.
    IPR001828. ANF_lig-bd_rcpt.
    IPR011009. Kinase-like_dom.
    IPR028082. Peripla_BP_I.
    IPR000719. Prot_kinase_dom.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF01094. ANF_receptor. 1 hit.
    PF00211. Guanylate_cyc. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view]
    SMARTiSM00044. CYCc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53822. SSF53822. 1 hit.
    SSF55073. SSF55073. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
    PS50125. GUANYLATE_CYCLASE_2. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5SDA5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFLGPWPFSR LLSWFAISSR LSGQHGLPSS KFLRCLCLLA LLPLLRWGQA     50
    LPYKIGVIGP WTCDPFFSKA LPEVAAALAI ERISRDKTFD RSYSFEYVIL 100
    NEDCQTSKAL ASFISHQQMA SGFVGPANPG FCEAASLLGT SWDKGIFSWA 150
    CVNHELDNKH SFPTFSRTLP SPIRVLVTVM KYFQWAHAGV ISSDEDIWMH 200
    TANRVSSALR SQGLPVGVVL TSGRDSQSIQ KALQQIRQAD RIRIIIMCMH 250
    SALIGGETQT HFLELAHDLK MTDGTYVFVP YDVLLYSLPY KHSPYQVLRN 300
    NPKLREAYDA VLTITVESHE KTFYEAYAEA AARGEIPEKP DSNQVSPLFG 350
    TIYNSIYFIA QAMNNAMKKN GRASAASLVQ HSRNMQFYGF NQLIKTDSNG 400
    NGISEYVILD TNGKEWELRG TYTVDMETEL LRFRGTPIHF PGGRPTSADA 450
    KCWFAERKIC QGGIDPALAM MVCFALLIAL LSINGFAYFI RRRINKIQLI 500
    KGPNRILLTL EDVTFINPHF GSKRGSRASV SFQIISEVQS GRSPRLSFSS 550
    GSLTPATYEN SNIAIYEGDW VWLKKFPPGD FGDIKSIKSS ASDVFEMMKD 600
    LRHENVNPLL GFFYDSGMFA IVSEFCSRRS LEDILTNDDV KLDWMFKSSL 650
    LLDLIKGMKY LHHREFIHGR LKSRNCVVDG RFVLKVTDYG FNDILEMLRL 700
    SEEEPSEEEL LWTAPELLRA PGGIRLGSFA GDVYSFAIIM QEVMVRGAPF 750
    CMMDLPAKEI IDRLKMPPPV YRPVVSPEYA PAECLQLMKQ CWAEASEQRP 800
    TFDEIFNQFK TFNKGKKTNI IDSMLRMLEQ YSSNLEDLIR ERTEELEIEK 850
    QKTEKLLTQM LPLSVAESLK KGCTVEPEGF DLVTLYFSDI VGFTTISAMS 900
    EPIEVVDLLN DLYTLFDAII GSHDVYKVET IGDAYMVASG LPKRNGSRHA 950
    AEIANMSLDI LSSVGTFKMR HMPEVPVRIR IGLHSGPVVA GVVGLTMPRY 1000
    CLFGDTVNTA SRMESTGLPY RIHVSLSTVT ILQTLSEGYE VELRGRTELK 1050
    GKGTEETFWL VGKKGFTKPL PVPPPVGKDG QVGHGLQPAE IAAFQRRKAE 1100
    RQLVRNKP 1108
    Length:1,108
    Mass (Da):124,425
    Last modified:December 21, 2004 - v1
    Checksum:i8A1456FCB384FA90
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY651761 mRNA. Translation: AAV54098.1.
    AL671916, BX324191 Genomic DNA. Translation: CAM19986.1.
    BX324191, AL671916 Genomic DNA. Translation: CAM24899.1.
    BC115714 mRNA. Translation: AAI15715.1.
    AK044234 mRNA. Translation: BAC31833.1.
    CCDSiCCDS30446.1.
    RefSeqiNP_001007577.1. NM_001007576.2.
    XP_006528904.1. XM_006528841.1.
    UniGeneiMm.208511.

    Genome annotation databases

    EnsembliENSMUST00000042530; ENSMUSP00000044521; ENSMUSG00000042282.
    GeneIDi245650.
    KEGGimmu:245650.
    UCSCiuc009ulq.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY651761 mRNA. Translation: AAV54098.1 .
    AL671916 , BX324191 Genomic DNA. Translation: CAM19986.1 .
    BX324191 , AL671916 Genomic DNA. Translation: CAM24899.1 .
    BC115714 mRNA. Translation: AAI15715.1 .
    AK044234 mRNA. Translation: BAC31833.1 .
    CCDSi CCDS30446.1.
    RefSeqi NP_001007577.1. NM_001007576.2.
    XP_006528904.1. XM_006528841.1.
    UniGenei Mm.208511.

    3D structure databases

    ProteinModelPortali Q5SDA5.
    SMRi Q5SDA5. Positions 75-246, 596-855, 884-1060.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000044521.

    PTM databases

    PhosphoSitei Q5SDA5.

    Proteomic databases

    PRIDEi Q5SDA5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000042530 ; ENSMUSP00000044521 ; ENSMUSG00000042282 .
    GeneIDi 245650.
    KEGGi mmu:245650.
    UCSCi uc009ulq.2. mouse.

    Organism-specific databases

    CTDi 2986.
    MGIi MGI:105119. Gucy2f.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00730000110520.
    HOGENOMi HOG000293307.
    HOVERGENi HBG098487.
    InParanoidi Q5SDA5.
    KOi K12321.
    OMAi NSIYFIA.
    OrthoDBi EOG7QVM1V.
    PhylomeDBi Q5SDA5.
    TreeFami TF106338.

    Enzyme and pathway databases

    Reactomei REACT_188266. Inactivation, recovery and regulation of the phototransduction cascade.

    Miscellaneous databases

    NextBioi 386884.
    PROi Q5SDA5.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q5SDA5.
    CleanExi MM_GUCY2F.
    Genevestigatori Q5SDA5.

    Family and domain databases

    Gene3Di 3.30.70.1230. 1 hit.
    InterProi IPR001054. A/G_cyclase.
    IPR018297. A/G_cyclase_CS.
    IPR001828. ANF_lig-bd_rcpt.
    IPR011009. Kinase-like_dom.
    IPR028082. Peripla_BP_I.
    IPR000719. Prot_kinase_dom.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF01094. ANF_receptor. 1 hit.
    PF00211. Guanylate_cyc. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view ]
    SMARTi SM00044. CYCc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53822. SSF53822. 1 hit.
    SSF55073. SSF55073. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00452. GUANYLATE_CYCLASE_1. 1 hit.
    PS50125. GUANYLATE_CYCLASE_2. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Application of functional genomic technologies in a mouse model of retinal degeneration."
      Shearstone J.R., Wang Y.E., Clement A., Allaire N.E., Yang C., Worley D.S., Carulli J.P., Perrin S.
      Genomics 85:309-321(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Retina.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-846.
      Strain: C57BL/6J.
      Tissue: Retina.

    Entry informationi

    Entry nameiGUC2F_MOUSE
    AccessioniPrimary (citable) accession number: Q5SDA5
    Secondary accession number(s): Q8BLL8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 2007
    Last sequence update: December 21, 2004
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3