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Protein

D-ribitol-5-phosphate cytidylyltransferase

Gene

Ispd

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cytidylyltransferase required for protein O-linked mannosylation. Catalyzes the formation of CDP-ribitol nucleotide sugar from D-ribitol 5-phosphate. CDP-ribitol is a substrate of FKTN during the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Shows activity toward other pentose phosphate sugars and mediates formation of CDP-ribulose or CDP-ribose using CTP and ribulose-5-phosphate or ribose-5-phosphate, respectively. Not Involved in dolichol production.By similarity

Catalytic activityi

CTP + D-ribitol 5-phosphate = diphosphate + CDP-ribitol.By similarity
CTP + D-ribose 5-phosphate = diphosphate + CDP-ribose.By similarity
CTP + D-ribulose 5-phosphate = diphosphate + CDP-ribulose.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei57Transition state stabilizerBy similarity1
Sitei64Transition state stabilizerBy similarity1
Sitei203Positions substrate for the nucleophilic attackBy similarity1
Sitei261Positions substrate for the nucleophilic attackBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
D-ribitol-5-phosphate cytidylyltransferaseBy similarity (EC:2.7.7.40By similarity)
Alternative name(s):
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like proteinBy similarity
Isoprenoid synthase domain-containing proteinImported
Notch1-induced protein1 Publication
Gene namesi
Name:IspdImported
Synonyms:Nip1 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi1359368. Ispd.

Subcellular locationi

  • Cytoplasmcytosol By similarity

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003436991 – 447D-ribitol-5-phosphate cytidylyltransferaseAdd BLAST447

Proteomic databases

PaxDbiQ5S6T3.
PRIDEiQ5S6T3.

Expressioni

Gene expression databases

BgeeiENSRNOG00000006199.
ExpressionAtlasiQ5S6T3. baseline and differential.
GenevisibleiQ5S6T3. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008312.

Structurei

3D structure databases

ProteinModelPortaliQ5S6T3.
SMRiQ5S6T3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IIH2. Eukaryota.
COG1211. LUCA.
GeneTreeiENSGT00390000006412.
HOVERGENiHBG057782.
InParanoidiQ5S6T3.
KOiK21031.
OMAiCYNFVCV.
OrthoDBiEOG091G0JSI.
PhylomeDBiQ5S6T3.
TreeFamiTF328415.

Family and domain databases

CDDicd02516. CDP-ME_synthetase. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiView protein in InterPro
IPR034683. IspD/TarI.
IPR018294. ISPD_synthase_CS.
IPR029044. Nucleotide-diphossugar_trans.
PfamiView protein in Pfam
PF01128. IspD. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiView protein in PROSITE
PS01295. ISPD. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5S6T3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESGPCSRPA EPRHCVSGRA GAGLAFPAFP LSAAGAEPGS RIGTVAAVLP
60 70 80 90 100
AGGCGERMGV RTPKQFCRVL ERPLISYTLQ AMERVCWIKD IVVTVTGENM
110 120 130 140 150
EAMRSIIQRY GHKRISLAEA GATRHRSIFN GLKALAEDQP GCELTRPEVV
160 170 180 190 200
IIHDAVRPFV EEDILLRVVL AAKEHGAAGA IRPLVSTVVS PSADGHLDHS
210 220 230 240 250
LDRAKHRASE MPQAFHFDVI YEAYQKCSDF DLEFGTECLQ LALKYCHRKA
260 270 280 290 300
KLVEGTPDLW KVTYKQDLCA AEAMIKEKIS QEICVVVNTK DEESVGHLLE
310 320 330 340 350
EVLRNELNCI KVTSTVLDRT SGDIENFIDQ CYSFICVNVV SSESRETRKL
360 370 380 390 400
LSILEESSLP LLYPVVVVLV HCFDFTVVPL AQKMENLVWI RELAKEAKGR
410 420 430 440
NVLLSGVLLN HSQDEQKLQE SLVQSAAIIA ALVKERNSAL VGQLLVA
Length:447
Mass (Da):49,192
Last modified:December 21, 2004 - v1
Checksum:i63FC38515B4F8FDE
GO
Isoform 2 (identifier: Q5S6T3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-99: Missing.

Show »
Length:348
Mass (Da):38,840
Checksum:i4575730394E404E2
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0346741 – 99Missing in isoform 2. 1 PublicationAdd BLAST99

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY769991 mRNA. Translation: AAV53695.1.
AY769992 mRNA. Translation: AAV53696.1.
RefSeqiNP_001008387.1. NM_001008386.1. [Q5S6T3-1]
UniGeneiRn.119606.

Genome annotation databases

EnsembliENSRNOT00000008312; ENSRNOP00000008312; ENSRNOG00000006199. [Q5S6T3-1]
GeneIDi493574.
KEGGirno:493574.
UCSCiRGD:1359368. rat. [Q5S6T3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiISPD_RAT
AccessioniPrimary (citable) accession number: Q5S6T3
Secondary accession number(s): Q5S6T4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: December 21, 2004
Last modified: August 30, 2017
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families