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Protein

Isoprenoid synthase domain-containing protein

Gene

Ispd

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Required for protein O-linked mannosylation. Probably acts as a nucleotidyltransferase involved in synthesis of a nucleotide sugar. Required for dystroglycan O-mannosylation (By similarity).By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei57 – 571Transition state stabilizerBy similarity
Sitei64 – 641Transition state stabilizerBy similarity
Sitei203 – 2031Positions substrate for the nucleophilic attackBy similarity
Sitei261 – 2611Positions substrate for the nucleophilic attackBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoprenoid synthase domain-containing protein (EC:2.7.7.-)
Alternative name(s):
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like protein
Notch1-induced protein
Gene namesi
Name:Ispd
Synonyms:Nip
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi1359368. Ispd.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447Isoprenoid synthase domain-containing proteinPRO_0000343699Add
BLAST

Proteomic databases

PaxDbiQ5S6T3.
PRIDEiQ5S6T3.

Expressioni

Gene expression databases

BgeeiENSRNOG00000006199.
ExpressionAtlasiQ5S6T3. baseline and differential.
GenevisibleiQ5S6T3. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008312.

Structurei

3D structure databases

ProteinModelPortaliQ5S6T3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IspD family.Curated

Phylogenomic databases

eggNOGiENOG410IIH2. Eukaryota.
COG1211. LUCA.
GeneTreeiENSGT00390000006412.
HOVERGENiHBG057782.
InParanoidiQ5S6T3.
OMAiCYNFVCV.
OrthoDBiEOG091G0JSI.
PhylomeDBiQ5S6T3.
TreeFamiTF328415.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001228. IspD.
IPR018294. ISPD_synthase_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS01295. ISPD. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5S6T3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESGPCSRPA EPRHCVSGRA GAGLAFPAFP LSAAGAEPGS RIGTVAAVLP
60 70 80 90 100
AGGCGERMGV RTPKQFCRVL ERPLISYTLQ AMERVCWIKD IVVTVTGENM
110 120 130 140 150
EAMRSIIQRY GHKRISLAEA GATRHRSIFN GLKALAEDQP GCELTRPEVV
160 170 180 190 200
IIHDAVRPFV EEDILLRVVL AAKEHGAAGA IRPLVSTVVS PSADGHLDHS
210 220 230 240 250
LDRAKHRASE MPQAFHFDVI YEAYQKCSDF DLEFGTECLQ LALKYCHRKA
260 270 280 290 300
KLVEGTPDLW KVTYKQDLCA AEAMIKEKIS QEICVVVNTK DEESVGHLLE
310 320 330 340 350
EVLRNELNCI KVTSTVLDRT SGDIENFIDQ CYSFICVNVV SSESRETRKL
360 370 380 390 400
LSILEESSLP LLYPVVVVLV HCFDFTVVPL AQKMENLVWI RELAKEAKGR
410 420 430 440
NVLLSGVLLN HSQDEQKLQE SLVQSAAIIA ALVKERNSAL VGQLLVA
Length:447
Mass (Da):49,192
Last modified:December 21, 2004 - v1
Checksum:i63FC38515B4F8FDE
GO
Isoform 2 (identifier: Q5S6T3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-99: Missing.

Show »
Length:348
Mass (Da):38,840
Checksum:i4575730394E404E2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9999Missing in isoform 2. 1 PublicationVSP_034674Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY769991 mRNA. Translation: AAV53695.1.
AY769992 mRNA. Translation: AAV53696.1.
RefSeqiNP_001008387.1. NM_001008386.1. [Q5S6T3-1]
UniGeneiRn.119606.

Genome annotation databases

EnsembliENSRNOT00000008312; ENSRNOP00000008312; ENSRNOG00000006199. [Q5S6T3-1]
GeneIDi493574.
KEGGirno:493574.
UCSCiRGD:1359368. rat. [Q5S6T3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY769991 mRNA. Translation: AAV53695.1.
AY769992 mRNA. Translation: AAV53696.1.
RefSeqiNP_001008387.1. NM_001008386.1. [Q5S6T3-1]
UniGeneiRn.119606.

3D structure databases

ProteinModelPortaliQ5S6T3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008312.

Proteomic databases

PaxDbiQ5S6T3.
PRIDEiQ5S6T3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000008312; ENSRNOP00000008312; ENSRNOG00000006199. [Q5S6T3-1]
GeneIDi493574.
KEGGirno:493574.
UCSCiRGD:1359368. rat. [Q5S6T3-1]

Organism-specific databases

CTDi729920.
RGDi1359368. Ispd.

Phylogenomic databases

eggNOGiENOG410IIH2. Eukaryota.
COG1211. LUCA.
GeneTreeiENSGT00390000006412.
HOVERGENiHBG057782.
InParanoidiQ5S6T3.
OMAiCYNFVCV.
OrthoDBiEOG091G0JSI.
PhylomeDBiQ5S6T3.
TreeFamiTF328415.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

PROiQ5S6T3.

Gene expression databases

BgeeiENSRNOG00000006199.
ExpressionAtlasiQ5S6T3. baseline and differential.
GenevisibleiQ5S6T3. RN.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001228. IspD.
IPR018294. ISPD_synthase_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS01295. ISPD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiISPD_RAT
AccessioniPrimary (citable) accession number: Q5S6T3
Secondary accession number(s): Q5S6T4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: December 21, 2004
Last modified: September 7, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.