Q5S1S4 (CAH3_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbonic anhydrase 3
EC=4.2.1.1

Alternative name(s):
Carbonate dehydratase III
Carbonic anhydrase III
Short name=CA-III

Gene names

Name:

CA3

Organism

Sus scrofa (Pig) [Complete proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Reversible hydration of carbon dioxide.
Catalytic activity	H2CO3 = CO2 + H2O.
Cofactor	Zinc By similarity.
Enzyme regulation	Inhibited by acetazolamide By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	S-glutathionylated in hepatocytes under oxidative stress By similarity.
Sequence similarities	Belongs to the alpha-carbonic anhydrase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Lyase
PTM	Glutathionylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carbonate dehydratase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 260	259	Carbonic anhydrase 3		PRO_0000077428
Regions
	Region	64 – 67	4	Involved in proton transfer By similarity
	Region	198 – 199	2	Substrate binding By similarity
Sites
	Active site	127	1	By similarity
	Metal binding	94	1	Zinc; catalytic By similarity
	Metal binding	96	1	Zinc; catalytic By similarity
	Metal binding	119	1	Zinc; catalytic By similarity
Amino acid modifications
	Modified residue	182	1	S-glutathionyl cysteine By similarity
	Modified residue	187	1	S-glutathionyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5S1S4 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 3256EF36BF48FC2F	FASTA	260	29,411
	10 20 30 40 50 60 MAKEWGYADH NGPDHWHELY PIAKGDNQSP IELHTKDIKH DPSLLPWTAS YDPGSAKTIL 70 80 90 100 110 120 NNGKTCRVVF DDTYDRSMLR GGPLTAAYRL RQFHLHWGSS DDHGSEHTVD GVKYAAELHL 130 140 150 160 170 180 VHWNSKYNSF ATALKHPDGV AVVGIFLKIG REKGEFQLVL DALDKIKTKG KEAPFTNFNP 190 200 210 220 230 240 SCLFPACRDY WTYHGSFTTP PCEECIVWLL LKEPITVSSD QMAKLRSLYS SAENEPPVPL 250 260 VRNWRPPQPI KGRIVKASFK

References

[1]	"Molecular characterization and association analysis of porcine CA3." Wang H.L., Zhu Z.M., Wang H., Yang S.L., Zhao S.H., Li K. Cytogenet. Genome Res. 115:129-133(2006) [PubMed: 17065793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Isolation, prediction of one novel swine gene that is differentially expressed in the longissimus dorsi muscle tissues from landrace large white cross combination." Liu G.Y., Xiong Z.Y. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Characterization of the porcine carbonic anhydrase III (CA3) mRNA." Wu J., Deng C.Y., Xiong Y.Z. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.
[4]	"Characterization of the porcine carbonic anhydrase III (CA3) gene." Wu J., Deng C.Y., Xiong Y.Z., Zhou D.H. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ157048 Genomic DNA. Translation: ABA41599.1. AY789645 mRNA. Translation: AAV65838.1. AY789514 mRNA. Translation: AAV83540.1. DQ675018 Genomic DNA. Translation: ABG49150.1.
RefSeq	NP_001008688.1. NM_001008688.1.
UniGene	Ssc.10960.
3D structure databases
ProteinModelPortal	Q5S1S4.
SMR	Q5S1S4. Positions 2-260.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSSSCT00000006734; ENSSSCP00000006551; ENSSSCG00000006141.
GeneID	494016.
KEGG	ssc:494016.
Organism-specific databases
CTD	761.
Phylogenomic databases
GeneTree	ENSGT00560000076828.
HOVERGEN	HBG002837.
OMA	LQPWSVS.
OrthoDB	EOG4001JV.
Family and domain databases
InterPro	IPR001148. a_carbonic_anhydrase. IPR023561. Carbonic_anhydrase_a-class. IPR018338. Carbonic_anhydrase_a-class_CS. IPR018441. Carbonic_anhydrase_CA3. [Graphical view]
Gene3D	G3DSA:3.10.200.10. Euk_COanhd. 1 hit.
KO	K01672.
PANTHER	PTHR18952:SF29. Carbonic_anhydrase_CA3. 1 hit. PTHR18952. Euk_COanhd. 1 hit.
Pfam	PF00194. Carb_anhydrase. 1 hit. [Graphical view]
SMART	SM01057. Carb_anhydrase. 1 hit. [Graphical view]
SUPFAM	SSF51069. Euk_COanhd. 1 hit.
PROSITE	PS00162. ALPHA_CA_1. 1 hit. PS51144. ALPHA_CA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAH3_PIG

Accession

Primary (citable) accession number: Q5S1S4
Secondary accession number(s): Q0Z809

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 49 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program