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Protein

Sperm-associated antigen 17

Gene

Spag17

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the central pair apparatus of ciliary axonemes. Plays a critical role in the function and structure of motile cilia (PubMed:23418344, PubMed:15827353). May play a role in endochondral bone formation, most likely because of a function in primary cilia of chondrocytes and osteoblasts (PubMed:26017218).3 Publications

GO - Biological processi

  • axoneme assembly Source: MGI
  • cilium movement Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm-associated antigen 17
Alternative name(s):
Projection protein PF6 homolog
Gene namesi
Name:Spag17
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1921612. Spag17.

Subcellular locationi

GO - Cellular componenti

  • axonemal central apparatus Source: MGI
  • cytoplasm Source: MGI
  • microtubule Source: UniProtKB-KW
  • motile cilium Source: UniProtKB-KW
  • nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum, Microtubule

Pathology & Biotechi

Disruption phenotypei

Deficient mice die within 12 hours of birth with severe defects in motile cilia (PubMed:23418344). Mice also have skeletal malformations (PubMed:26017218).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 23202320Sperm-associated antigen 17PRO_0000331446Add
BLAST

Proteomic databases

PaxDbiQ5S003.
PRIDEiQ5S003.

PTM databases

iPTMnetiQ5S003.
PhosphoSiteiQ5S003.

Expressioni

Tissue specificityi

Highly expressed in testis, round spermatids, testicular sperm, epididymal sperm and in condensing spermatids (at protein level) (PubMed:15827353). Expressed in organs that contain cilia-bearing cells including brain, oviduct, lung, and uterus (PubMed:15827353). Expressed in articular cartilage and bone (PubMed:26017218).2 Publications

Developmental stagei

Expressed from day 16 when pachytene spermatocytes are present.1 Publication

Gene expression databases

GenevisibleiQ5S003. MM.

Interactioni

Subunit structurei

Interacts (via the C-terminus) with SPAG6; the interaction probably occurs on polymerized microtubules.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Spag6Q9JLI73EBI-1783665,EBI-1783654

Protein-protein interaction databases

IntActiQ5S003. 1 interaction.
STRINGi10090.ENSMUSP00000134066.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili865 – 965101Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi888 – 999112Lys-richPROSITE-ProRule annotationAdd
BLAST
Compositional biasi1080 – 113152Glu-richPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGU0. Eukaryota.
ENOG410ZGKQ. LUCA.
GeneTreeiENSGT00390000013693.
HOVERGENiHBG101382.
InParanoidiQ5S003.
OMAiYKTVMPP.
OrthoDBiEOG7DJSKM.

Family and domain databases

InterProiIPR026173. SPAG17.
[Graphical view]
PANTHERiPTHR21963. PTHR21963. 3 hits.

Sequencei

Sequence statusi: Complete.

Q5S003-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPKKEKPTG SANYKIWEPS LIAAHLNQND WKASIAFVVG NRVEDDLLIH
60 70 80 90 100
ALDLAVRLPQ RKLFSIVSWE DILQQMDEIQ SLAESASAKK GKKPTSVNLP
110 120 130 140 150
LHYEVFLAAK IIMESGEKLT LPLIGKLLKC QLLHIKSKDQ QRRENEKKMV
160 170 180 190 200
EERTKSEKDK GKGKSPKEKK VASAKPGKGK SKDQPEATVT VRKTTQLKRR
210 220 230 240 250
GEDDEAKSYI DDEPDDGAQY YIIVVGFNNP QLLAIMTELG IPITSVIKIS
260 270 280 290 300
SENYEPLQTH LAAVRQQQEA VLQPEDIEAE KLKRKNSIKE LEVFWKYLEP
310 320 330 340 350
ILNNEKLEIH LFDVARLQYM VKATYFPSDW SDNEQMLALG TEIFENIACL
360 370 380 390 400
MYDSLDWKRQ HHHYLQSMQL INVPQVVSEK TVLEAITIPE PPPSTAPAPT
410 420 430 440 450
GKKKAQYEES HAPPTVAFII TTEVDMRYYN DLLNPIPEEF ISVSLILHCM
460 470 480 490 500
VEQVVATEED LIPPSLVEPA PRADGLDYRI AAHIVSILPS LCLSEKEKKN
510 520 530 540 550
LREIFLTEGE SESKALPKGP LLLNYHDAHA HKKYALKDQK NFDPVQVEQE
560 570 580 590 600
MQSKLPLWEF LQFPLPPPWN STKRLATIHE LMHFCTNEVL SWNEVERAFK
610 620 630 640 650
VFTFESLKLS EVDEEGRLKP TETTSDTDVE NFNIPWDNPA RFAKLIRQRY
660 670 680 690 700
IHRMSMQKAP PVVVEIENTE RTLFVNKNFA KAEQDAQGDE NSPNSDEPDA
710 720 730 740 750
ISVTGSTSNS TKPWNSSNRQ FSEKETSGSM WPQPESMDQT MDTEIKDDAA
760 770 780 790 800
TKDDSPEKKP KKMVVEADIE DIKKTQQRSL MDWSFTEYFQ PKVLLQVLQE
810 820 830 840 850
AHQQYRCVDS YYHTQDNSLL LVFHNPMNLQ RLQCEHWNIA LHSNVGFRNY
860 870 880 890 900
LELVAKSIED WVTQEEAKYQ EAKMAEELNR IRIELELKAT VKTSASKIPG
910 920 930 940 950
PKRSKTNKVS SKTELSDQEK DKEKEKDKIP FVLEGSLKAW KEEQERLAEE
960 970 980 990 1000
ERLKEEKKAE KKGKDTGKKK GKDKADKDDA KALKKKSSSK EKPKEEPAKT
1010 1020 1030 1040 1050
LEVIEETAPL PVPEVVYPFR GYNMGDIPIQ ISGTNYYLYP SDGGQIEVEK
1060 1070 1080 1090 1100
TRFERGSTFI KVKVKKDKHN FIIHLKDPKE IVKKEKEEKN SEEEEEEEEE
1110 1120 1130 1140 1150
KEEVEEKKPK EGEEEKVKQK VEMKRAQIEK EAVSKFGSFS ATLENGICLS
1160 1170 1180 1190 1200
ISYYGSNGMA PEVINSELEA MMNIPSAMTA TVVPAVVTVP QGKGKAKPKG
1210 1220 1230 1240 1250
KEKHKDSIKE EELPKEEEKK NHIQEEVEPE IVIQESPPYV PTFQNLNVSC
1260 1270 1280 1290 1300
PSGLLLTFIG QESTDYSIVD EEPTRNLMIR QSYPQRLKHY EFYKAVMPPL
1310 1320 1330 1340 1350
EQEASRVVTS QGTVIKYMLD GSTQILFADG AVSSSPDSGP VYTSPELPTS
1360 1370 1380 1390 1400
PHNGDLVDSA SQPKSETGPE IIITKKGKGH KNQTVANKSE THDIIPEVPP
1410 1420 1430 1440 1450
PTPVESHIGT WFTTTPDGRR IGTKGLEKIE DLKPYLFFQA TDPINGTVMT
1460 1470 1480 1490 1500
TREDKVIIVE KKDGTRVVDH ADGTRITTFY QVYEDHITPS NDEETTEGPR
1510 1520 1530 1540 1550
TVTRQVKCMR IESSHYATII TNCEDSSCCA TFGDGTSIIA KPQGSYQVLP
1560 1570 1580 1590 1600
PNTGCLYIDK DCSATYCHES SNNLYHPFQK REQLRASRYI MKHTSEVICE
1610 1620 1630 1640 1650
VQDPEGNTFQ VMADGSVSTT LPKKKLEDDF NVQMEGYESL SSLHLEKNHM
1660 1670 1680 1690 1700
QIYGEHVPRF FVVYTDGSGV ELLRDSDIEE YLSLAYGEST TVVLQEPVQE
1710 1720 1730 1740 1750
YPGALSITVL RPFHEASQWI MKKELDTIVP PNLQSRSWER FPSVEKKTPG
1760 1770 1780 1790 1800
PPFGTQIWKG LSIGSKQLTN IPAPILEGPK VLQMRQFIQH EVIKNEVKLK
1810 1820 1830 1840 1850
LQISLKDYIN HILKKEDELQ EMTVKDSRTE EERGNAADLL KLVMSFPKME
1860 1870 1880 1890 1900
ETTKSHMTKV AAHLTVLFKQ SMASAPKCSP DSYSKEFLEK KWRSLSQGTS
1910 1920 1930 1940 1950
WKEKLEQQRN NIKKTQSYLM QIKTKEVTPY FKSELSSLFK SKYDYLEKFS
1960 1970 1980 1990 2000
KSLPPFVKKN EAKLMTAVPD LYSDSTLTVD TEKEASNTHP LLNQEVAENI
2010 2020 2030 2040 2050
QESPREKETE YVNKSLQTSS SQNQYENVTI SPKESVYQSQ TEIETKDTKE
2060 2070 2080 2090 2100
SAIQNFTEKF KKYTKKSASQ NEIEDLIKST KESVSQRQTE NVTRPPTEEP
2110 2120 2130 2140 2150
DIYMPIKIPT QSLLQDVTGQ ARKEKVRLPY YMMSSKPKSQ PYAKVEDPVG
2160 2170 2180 2190 2200
GRVNTSSIAS AAMYNPNASP FGFHLLPPSV KFGVLKEGHT YATIVKLKNV
2210 2220 2230 2240 2250
GVDFCRFRVK QPPPSTGLKV TYKPGPVAAG LQAELKVELF AMAVGEDGAK
2260 2270 2280 2290 2300
GSAHISHNIE IMTEHDVLFL PVEATVLTSS NYDNRPKNLP QGKENPMVFR
2310 2320
TSTISSSSLG VVMSQKATHH
Length:2,320
Mass (Da):263,102
Last modified:January 7, 2015 - v2
Checksum:i2AC5D4505F7514B5
GO

Sequence cautioni

The sequence AAS66754.1 differs from that shown. Reason: Frameshift at position 872. Curated
The sequence AAV66533.1 differs from that shown.Missing sequence in a highly repetitive region between exons 21 and 22.Curated
The sequence BAC26566.1 differs from that shown. Reason: Frameshift at positions 181 and 872. Curated
The sequence BAE22947.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti193 – 1931K → N in BAC26566 (PubMed:16141072).Curated
Sequence conflicti381 – 3811T → S in BAC26566 (PubMed:16141072).
Sequence conflicti788 – 7881Y → C in AAV66533 (PubMed:15827353).Curated
Sequence conflicti788 – 7881Y → C in AAS66754 (PubMed:15827353).Curated
Sequence conflicti788 – 7881Y → C in BAC26566 (PubMed:16141072).Curated
Sequence conflicti1629 – 16291D → N in AAV66533 (PubMed:15827353).Curated
Sequence conflicti2029 – 20291T → P in BAE22947 (PubMed:16141072).Curated
Sequence conflicti2082 – 20821E → G in BAE22947 (PubMed:16141072).Curated
Sequence conflicti2212 – 22121P → S in AAV66533 (PubMed:15827353).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY555275 mRNA. Translation: AAS66754.1. Frameshift.
AY792594 mRNA. Translation: AAV66533.1. Sequence problems.
AC102209 Genomic DNA. No translation available.
AC167172 Genomic DNA. No translation available.
AL606749 Genomic DNA. No translation available.
AK016477 mRNA. Translation: BAB30259.1.
AK029691 mRNA. Translation: BAC26566.1. Frameshift.
AK136364 mRNA. Translation: BAE22947.1. Different initiation.
CCDSiCCDS57248.1.
RefSeqiNP_083168.3. NM_028892.4.
UniGeneiMm.55847.

Genome annotation databases

EnsembliENSMUST00000164539; ENSMUSP00000134066; ENSMUSG00000027867.
GeneIDi74362.
KEGGimmu:74362.
UCSCiuc012cus.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY555275 mRNA. Translation: AAS66754.1. Frameshift.
AY792594 mRNA. Translation: AAV66533.1. Sequence problems.
AC102209 Genomic DNA. No translation available.
AC167172 Genomic DNA. No translation available.
AL606749 Genomic DNA. No translation available.
AK016477 mRNA. Translation: BAB30259.1.
AK029691 mRNA. Translation: BAC26566.1. Frameshift.
AK136364 mRNA. Translation: BAE22947.1. Different initiation.
CCDSiCCDS57248.1.
RefSeqiNP_083168.3. NM_028892.4.
UniGeneiMm.55847.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ5S003. 1 interaction.
STRINGi10090.ENSMUSP00000134066.

PTM databases

iPTMnetiQ5S003.
PhosphoSiteiQ5S003.

Proteomic databases

PaxDbiQ5S003.
PRIDEiQ5S003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000164539; ENSMUSP00000134066; ENSMUSG00000027867.
GeneIDi74362.
KEGGimmu:74362.
UCSCiuc012cus.1. mouse.

Organism-specific databases

CTDi200162.
MGIiMGI:1921612. Spag17.

Phylogenomic databases

eggNOGiENOG410IGU0. Eukaryota.
ENOG410ZGKQ. LUCA.
GeneTreeiENSGT00390000013693.
HOVERGENiHBG101382.
InParanoidiQ5S003.
OMAiYKTVMPP.
OrthoDBiEOG7DJSKM.

Miscellaneous databases

ChiTaRSiSpag17. mouse.
NextBioi340549.
PROiQ5S003.
SOURCEiSearch...

Gene expression databases

GenevisibleiQ5S003. MM.

Family and domain databases

InterProiIPR026173. SPAG17.
[Graphical view]
PANTHERiPTHR21963. PTHR21963. 3 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Dissecting the axoneme interactome: the mammalian orthologue of Chlamydomonas PF6 interacts with sperm-associated antigen 6, the mammalian orthologue of Chlamydomonas PF16."
    Zhang Z., Jones B.H., Tang W., Moss S.B., Wei Z., Ho C., Pollack M., Horowitz E., Bennett J., Baker M.E., Strauss J.F. III
    Mol. Cell. Proteomics 4:914-923(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH SPAG6, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6JImported.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-939 AND 1808-2320.
    Strain: C57BL/6J.
    Tissue: Lung and Testis.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.
  5. Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  6. Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiSPG17_MOUSE
AccessioniPrimary (citable) accession number: Q5S003
Secondary accession number(s): G3UYF7
, Q3UWG8, Q6Q758, Q8CDR5, Q9CUE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: January 7, 2015
Last modified: January 20, 2016
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.