Q5RLY7 (CDO_AJECG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine dioxygenase
Short name=CDO
EC=1.13.11.20

Gene names

Name:	CDO1
ORF Names:	HCBG_06968

Organism

Ajellomyces capsulata (strain G186AR / H82 / ATCC MYA-2454 / RMSCC 2432) (Darling's disease fungus) (Histoplasma capsulatum) [Reference proteome]

Taxonomic identifier

447093 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Onygenales › Ajellomycetaceae › Ajellomyces › Ajellomyces capsulatus

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	L-cysteine + O2 = 3-sulfinoalanine.
Cofactor	Binds 1 iron ion per subunit By similarity.
Pathway	Organosulfur biosynthesis; taurine biosynthesis; hypotaurine from L-cysteine: step 1/2.
Post-translational modification	The thioether cross-link between Cys-107 and Tyr-177 plays a structural role through stabilizing the Fe2+ ion, and prevents the production of highly damaging free hydroxyl radicals by holding the oxygen radical via hydroxyl hydrogen By similarity.
Sequence similarities	Belongs to the cysteine dioxygenase family.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
PTM	Thioether bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-cysteine metabolic process Inferred from electronic annotation. Source: InterPro taurine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	cysteine dioxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	Cysteine dioxygenase		PRO_0000206614
Sites
	Metal binding	100	1	Iron; catalytic By similarity
	Metal binding	102	1	Iron; catalytic By similarity
	Metal binding	160	1	Iron; catalytic By similarity
Amino acid modifications
	Cross-link	107 ↔ 177		3'-(S-cysteinyl)-tyrosine (Cys-Tyr) By similarity
Experimental info
	Sequence conflict	146	1	K → E in AAV66535. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5RLY7 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: 62C0639517F657FB	FASTA	213	23,768
	10 20 30 40 50 60 MPYLENSESS PDPTPLDAFH CLVQDINKVL GPSSGLDSDD VDPMDIQKLM EDYTSNESEW 70 80 90 100 110 120 ERYAFGDAGR AYTRNLVDEG NGKCNLLILV WSPGKGSAIH DHANAHCVMK VLKGSLRETL 130 140 150 160 170 180 YGWPESDKVQ KGEPSPLTVT RDKVYKEGQV TYMSDKLGLH KISNPDPTNF AISLHLYTPP 190 200 210 NAAHYGFSLF DEKTGKSRHV KQSVLFSRKG HKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the cysteine dioxygenase gene (CDO1) from the dimorphic pathogenic fungus Histoplasma capsulatum." Hasler S., Shearer G. Jr. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of Ajellomyces capsulatus strain G186AR." Champion M., Cuomo C.A., Ma L.-J., Henn M.R., Sil A., Goldman B., Young S.K., Kodira C.D., Zeng Q., Koehrsen M., Alvarado L., Berlin A., Borenstein D., Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J. , Griggs A., Gujja S., Heiman D., Hepburn T., Howarth C., Jen D., Larson L., Lewis B., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., Yandava C., Klein B., McEwen J.G., Puccia R., Goldman G.H., Felipe M.S., Nino-Vega G., San-Blas G., Taylor J., Mendoza L., Galagan J.E., Nusbaum C., Birren B.W. Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: G186AR / H82 / ATCC MYA-2454 / RMSCC 2432.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY804144 mRNA. Translation: AAV66535.1. GG663372 Genomic DNA. Translation: EEH05017.1.
3D structure databases
ProteinModelPortal	Q5RLY7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
OrthoDB	EOG44F9KG.
Enzyme and pathway databases
UniPathway	UPA00012; UER00537.
Family and domain databases
Gene3D	2.60.120.10. 1 hit.
InterPro	IPR010300. Cys_dOase_I. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
Pfam	PF05995. CDO_I. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CDO_AJECG

Accession

Primary (citable) accession number: Q5RLY7
Secondary accession number(s): C0NUD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	June 16, 2009
Last modified:	March 6, 2013
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program