Reviewed, UniProtKB/Swiss-Prot Q5RLY7 (CDO_AJECG)

Names and origin

Protein names

Recommended name:
    Cysteine dioxygenase
      Short name=CDO
    EC=1.13.11.20

Gene names

Name:	CDO1
ORF Names:	HCBG_06968

Organism

Ajellomyces capsulata (strain ATCC 26029 / G186AR / H82 / RMSCC 2432) (Darling's disease fungus) (Histoplasma capsulatum)

Taxonomic identifier

447093 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › Ajellomycetaceae › Ajellomyces

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-cysteine + O2 = 3-sulfinoalanine.
Cofactor	Binds 1 iron ion per subunit By similarity.
Pathway	Organosulfur biosynthesis; taurine biosynthesis; hypotaurine from L-cysteine: step 1/2.
Post-translational modification	The thioether cross-link between Cys-107 and Tyr-177 plays a structural role through stabilizing the Fe2+ ion, and prevents the production of highly damaging free hydroxyl radicals by holding the oxygen radical via hydroxyl hydrogen By similarity.
Sequence similarities	Belongs to the cysteine dioxygenase family.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
PTM	Thioether bond
Gene Ontology (GO)
Biological process	L-cysteine metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cysteine dioxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	Cysteine dioxygenase		PRO_0000206614
Sites
	Metal binding	100	1	Iron; catalytic By similarity
	Metal binding	102	1	Iron; catalytic By similarity
	Metal binding	160	1	Iron; catalytic By similarity
Amino acid modifications
	Cross-link	107 ↔ 177		3'-(S-cysteinyl)-tyrosine (Cys-Tyr) By similarity
Experimental info
	Sequence conflict	146	1	K → E in AAV66535. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5RLY7-1 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: 62C0639517F657FB	FASTA	213	23,768
	10 20 30 40 50 60 MPYLENSESS PDPTPLDAFH CLVQDINKVL GPSSGLDSDD VDPMDIQKLM EDYTSNESEW 70 80 90 100 110 120 ERYAFGDAGR AYTRNLVDEG NGKCNLLILV WSPGKGSAIH DHANAHCVMK VLKGSLRETL 130 140 150 160 170 180 YGWPESDKVQ KGEPSPLTVT RDKVYKEGQV TYMSDKLGLH KISNPDPTNF AISLHLYTPP 190 200 210 NAAHYGFSLF DEKTGKSRHV KQSVLFSRKG HKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the cysteine dioxygenase gene (CDO1) from the dimorphic pathogenic fungus Histoplasma capsulatum." Hasler S., Shearer G. Jr. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of Ajellomyces capsulatus strain G186AR." Champion M., Cuomo C.A., Ma L.-J., Henn M.R., Sil A., Goldman B., Young S.K., Kodira C.D., Zeng Q., Koehrsen M., Alvarado L., Berlin A., Borenstein D., Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J. , Griggs A., Gujja S., Heiman D., Hepburn T., Howarth C., Jen D., Larson L., Lewis B., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., Yandava C., Klein B., McEwen J.G., Puccia R., Goldman G.H., Felipe M.S., Nino-Vega G., San-Blas G., Taylor J., Mendoza L., Galagan J.E., Nusbaum C., Birren B.W. Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY804144 mRNA. Translation: AAV66535.1. GG663372 Genomic DNA. Translation: EEH05017.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.13.11.20. 266116.
Family and domain databases
InterPro	IPR011051. Cupin_RmlC_type. IPR010300. Cys_dOase_I. [Graphical view]
Pfam	PF05995. CDO_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDO_AJECG

Accession

Primary (citable) accession number: Q5RLY7
Secondary accession number(s): C0NUD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	June 16, 2009
Last modified:	November 24, 2009
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)