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Protein

Solute carrier family 22 member 7

Gene

Slc22a7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates sodium-independent multispecific organic anion transport. Transport of ketoprofen, indomethacin, salicylate, acetylsalicylate, prostaglandin E2, 3'-azido-3'-deoxythymidine (AZT), 2',3'-dideoxycytidine (ddC), dicarboxylates and p-aminohippurate (PAH).3 Publications

Kineticsi

  1. KM=0.37 µM for indomethacin1 Publication
  2. KM=26 µM for AZT1 Publication
  3. KM=3.08 mM for ddC1 Publication

    GO - Molecular functioni

    • organic anion transmembrane transporter activity Source: RGD

    GO - Biological processi

    • organic anion transport Source: RGD
    Complete GO annotation...

    Keywords - Biological processi

    Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiR-RNO-561048. Organic anion transport.

    Protein family/group databases

    TCDBi2.A.1.19.15. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Solute carrier family 22 member 7
    Alternative name(s):
    Novel liver transporter
    Organic anion transporter 2
    Gene namesi
    Name:Slc22a7
    Synonyms:Nlt, Oat2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Chromosome 9

    Organism-specific databases

    RGDi621699. Slc22a7.

    Subcellular locationi

    • Basolateral cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    • Note: Apical side of the renal tubule.

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei21 – 4121HelicalSequence analysisAdd
    BLAST
    Transmembranei144 – 16421HelicalSequence analysisAdd
    BLAST
    Transmembranei178 – 19821HelicalSequence analysisAdd
    BLAST
    Transmembranei202 – 22221HelicalSequence analysisAdd
    BLAST
    Transmembranei232 – 25221HelicalSequence analysisAdd
    BLAST
    Transmembranei257 – 27721HelicalSequence analysisAdd
    BLAST
    Transmembranei344 – 36421HelicalSequence analysisAdd
    BLAST
    Transmembranei375 – 39521HelicalSequence analysisAdd
    BLAST
    Transmembranei402 – 42221HelicalSequence analysisAdd
    BLAST
    Transmembranei429 – 44921HelicalSequence analysisAdd
    BLAST
    Transmembranei464 – 48421HelicalSequence analysisAdd
    BLAST
    Transmembranei489 – 50921HelicalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    • basolateral plasma membrane Source: UniProtKB-SubCell
    • integral component of plasma membrane Source: RGD
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Chemistry

    ChEMBLiCHEMBL2073671.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 535535Solute carrier family 22 member 7PRO_0000317486Add
    BLAST

    Proteomic databases

    PaxDbiQ5RLM2.
    PRIDEiQ5RLM2.

    Expressioni

    Tissue specificityi

    Expressed in liver and kidney.1 Publication

    Gene expression databases

    BgeeiENSRNOG00000018420.
    GenevisibleiQ5RLM2. RN.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000025094.

    Chemistry

    BindingDBiQ5RLM2.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5RLM2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG0255. Eukaryota.
    COG0477. LUCA.
    GeneTreeiENSGT00760000118852.
    HOGENOMiHOG000234569.
    HOVERGENiHBG108433.
    InParanoidiQ5RLM2.
    KOiK08204.
    OMAiASINYIM.
    OrthoDBiEOG091G07GO.
    PhylomeDBiQ5RLM2.
    TreeFamiTF315847.

    Family and domain databases

    CDDicd06174. MFS. 1 hit.
    InterProiIPR020846. MFS_dom.
    IPR005828. MFS_sugar_transport_like.
    IPR004749. Orgcat_transp/SVOP.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00898. 2A0119. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5RLM2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGFEDLLDKV GGFGPFQLRN LVLMALPRML LPMHFLLPVF MAAVPAHHCA
    60 70 80 90 100
    LPGAPANLSH QDLWLEAHLP RETDGSFSSC LRFAYPQTVP NVTLGTEVSN
    110 120 130 140 150
    SGEPEGEPLT VPCSQGWEYD RSEFSSTIAT EWDLVCQQRG LNKITSTCFF
    160 170 180 190 200
    IGVLVGAVVY GYLSDRFGRR RLLLVAYVSS LVLGLMSAAS INYIMFVVTR
    210 220 230 240 250
    TLTGSALAGF TIIVLPLELE WLDVEHRTVA GVISTVFWSG GVLLLALVGY
    260 270 280 290 300
    LIRSWRWLLL AATLPCVPGI ISIWWVPESA RWLLTQGRVE EAKKYLLSCA
    310 320 330 340 350
    KLNGRPVGEG SLSQEALNNV VTMERALQRP SYLDLFRTSQ LRHISLCCMM
    360 370 380 390 400
    VWFGVNFSYY GLTLDVSGLG LNVYQTQLLF GAVELPSKIM VYFLVRRLGR
    410 420 430 440 450
    RLTEAGMLLG AALTFGTSLL VSLETKSWIT ALVVVGKAFS EAAFTTAYLF
    460 470 480 490 500
    TSELYPTVLR QTGLGLTALM GRLGASLAPL AALLDGVWLL LPKVAYGGIA
    510 520 530
    LVAACTALLL PETKKAQLPE TIQDVERKST QEEDV
    Length:535
    Mass (Da):58,654
    Last modified:December 21, 2004 - v1
    Checksum:iE7C38969DCAE2B73
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti479 – 4791P → R in AAA57157 (PubMed:8056831).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L27651 mRNA. Translation: AAA57157.1.
    AY816233 mRNA. Translation: AAV66454.1.
    RefSeqiNP_445989.2. NM_053537.2.
    UniGeneiRn.10009.

    Genome annotation databases

    EnsembliENSRNOT00000025094; ENSRNOP00000025094; ENSRNOG00000018420.
    GeneIDi89776.
    KEGGirno:89776.
    UCSCiRGD:621699. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L27651 mRNA. Translation: AAA57157.1.
    AY816233 mRNA. Translation: AAV66454.1.
    RefSeqiNP_445989.2. NM_053537.2.
    UniGeneiRn.10009.

    3D structure databases

    ProteinModelPortaliQ5RLM2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000025094.

    Chemistry

    BindingDBiQ5RLM2.
    ChEMBLiCHEMBL2073671.

    Protein family/group databases

    TCDBi2.A.1.19.15. the major facilitator superfamily (mfs).

    Proteomic databases

    PaxDbiQ5RLM2.
    PRIDEiQ5RLM2.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSRNOT00000025094; ENSRNOP00000025094; ENSRNOG00000018420.
    GeneIDi89776.
    KEGGirno:89776.
    UCSCiRGD:621699. rat.

    Organism-specific databases

    CTDi10864.
    RGDi621699. Slc22a7.

    Phylogenomic databases

    eggNOGiKOG0255. Eukaryota.
    COG0477. LUCA.
    GeneTreeiENSGT00760000118852.
    HOGENOMiHOG000234569.
    HOVERGENiHBG108433.
    InParanoidiQ5RLM2.
    KOiK08204.
    OMAiASINYIM.
    OrthoDBiEOG091G07GO.
    PhylomeDBiQ5RLM2.
    TreeFamiTF315847.

    Enzyme and pathway databases

    ReactomeiR-RNO-561048. Organic anion transport.

    Miscellaneous databases

    PROiQ5RLM2.

    Gene expression databases

    BgeeiENSRNOG00000018420.
    GenevisibleiQ5RLM2. RN.

    Family and domain databases

    CDDicd06174. MFS. 1 hit.
    InterProiIPR020846. MFS_dom.
    IPR005828. MFS_sugar_transport_like.
    IPR004749. Orgcat_transp/SVOP.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00898. 2A0119. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiS22A7_RAT
    AccessioniPrimary (citable) accession number: Q5RLM2
    Secondary accession number(s): Q63314
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: December 21, 2004
    Last modified: September 7, 2016
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.