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Protein

Molybdenum cofactor biosynthesis protein 1

Gene

Mocs1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform Mocs1a and isoform Mocs1b probably form a complex that catalyzes the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP). Mocs1a catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate and Mocs1b catalyzes the subsequent conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cPMP.By similarity

Catalytic activityi

GTP + S-adenosyl-L-methionine + reduced electron acceptor = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor.By similarity
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate.By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.By similarity

Pathwayi: molybdopterin biosynthesis

This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei73GTPBy similarity1
Metal bindingi80Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi84Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Binding sitei86S-adenosyl-L-methionineBy similarity1
Metal bindingi87Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Binding sitei123GTPBy similarity1
Binding sitei127S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei154GTPBy similarity1
Binding sitei178S-adenosyl-L-methionineBy similarity1
Binding sitei215GTPBy similarity1
Binding sitei249S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1
Metal bindingi312Iron-sulfur 2 (4Fe-4S-substrate)By similarity1
Metal bindingi315Iron-sulfur 2 (4Fe-4S-substrate)By similarity1
Metal bindingi329Iron-sulfur 2 (4Fe-4S-substrate)By similarity1
Active sitei606For molybdenum cofactor biosynthesis protein C activitySequence analysis1

GO - Molecular functioni

GO - Biological processi

  • molybdopterin cofactor biosynthetic process Source: MGI
  • Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Molybdenum cofactor biosynthesis

Keywords - Ligandi

4Fe-4S, GTP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-947581. Molybdenum cofactor biosynthesis.
UniPathwayiUPA00344.

Names & Taxonomyi

Protein namesi
Recommended name:
Molybdenum cofactor biosynthesis protein 1
Including the following 2 domains:
GTP 3',8-cyclase (EC:4.1.99.22By similarity)
Alternative name(s):
Molybdenum cofactor biosynthesis protein A
Cyclic pyranopterin monophosphate synthase (EC:4.6.1.17By similarity)
Alternative name(s):
Molybdenum cofactor biosynthesis protein C
Gene namesi
Name:Mocs1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1928904. Mocs1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Death between days 1 and 11 after birth, due to a progressive neurological disorder caused by massive cell death. Death is caused by the absence of molybdenum cofactor, resulting in elevated sulfite and diminished sulfate levels throughout the organism. Mice do not possess any sulfite oxidase or xanthine dehydrogenase activity. No organ abnormalities are observed and the synaptic localization of inhibitory receptors appears normal. Long-term rescue results have been obtained in mice lacking Mocs1 thanks to an adeno-associated virus-mediated gene transfer.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003694001 – 636Molybdenum cofactor biosynthesis protein 1Add BLAST636

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei64PhosphoserineBy similarity1
Modified residuei198N6-acetyllysineBy similarity1
Modified residuei528N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5RKZ7.
PeptideAtlasiQ5RKZ7.
PRIDEiQ5RKZ7.

PTM databases

iPTMnetiQ5RKZ7.
PhosphoSitePlusiQ5RKZ7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000064120.
ExpressionAtlasiQ5RKZ7. baseline and differential.
GenevisibleiQ5RKZ7. MM.

Interactioni

Subunit structurei

Isoform Mocs1a and isoform Mocs1b probably form a heterooligomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000133694.

Structurei

3D structure databases

ProteinModelPortaliQ5RKZ7.
SMRiQ5RKZ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 383Molybdenum cofactor biosynthesis protein AAdd BLAST383
Regioni317 – 319GTP bindingBy similarity3
Regioni414 – 636Molybdenum cofactor biosynthesis protein CAdd BLAST223

Sequence similaritiesi

In the C-terminal section; belongs to the MoaC family.Curated
In the N-terminal section; belongs to the MoaA/NifB/PqqE family.Curated

Phylogenomic databases

eggNOGiKOG2876. Eukaryota.
COG0315. LUCA.
COG2896. LUCA.
GeneTreeiENSGT00390000016567.
HOGENOMiHOG000228683.
InParanoidiQ5RKZ7.
KOiK03639.
OMAiDMCKAVT.
OrthoDBiEOG091G0HQS.
PhylomeDBiQ5RKZ7.
TreeFamiTF300424.

Family and domain databases

CDDicd01420. MoaC_PE. 1 hit.
Gene3Di3.20.20.70. 1 hit.
3.30.70.640. 1 hit.
HAMAPiMF_01225_B. MoaA_B. 1 hit.
MF_01224_B. MoaC_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR023045. Mo_CF_biosynth-C.
IPR023046. Mo_CF_biosynth-C_bac.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR002820. Mopterin_CF_biosynth-C_dom.
IPR007197. rSAM.
[Graphical view]
PfamiPF01967. MoaC. 1 hit.
PF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
SUPFAMiSSF55040. SSF55040. 1 hit.
TIGRFAMsiTIGR02666. moaA. 1 hit.
TIGR00581. moaC. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Mocs1b (identifier: Q5RKZ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAARPAFGIV RQLLRSNARG CSSGAPVTQP RPGEPSRPTR EGLSLRLQFL
60 70 80 90 100
QEHAAPFSAF LTDSFGRQHS YLRISLTEKC NLRCQYCMPE EGVPLTPKAD
110 120 130 140 150
LLTTEEILTL ARLFVKEGVD KIRLTGGEPL IRPDVVDIVA RLHGLEGLRT
160 170 180 190 200
IGLTTNGINL ARLLPRLQQA GLNAVNISLD TLVPAKFEFI VRRKGFHKVM
210 220 230 240 250
EGIHKAIELG YKPVKVNCVV MRGLNEDELL DFVALTEGLP LDVRFIEYMP
260 270 280 290 300
FDGNKWNFKK MVSYKEMLDT IRQRWPGLEK LPEEDSSTAK AFKIPGFQGQ
310 320 330 340 350
ISFITSMSEH FCGTCNRLRI TADGNLKVCL FGNSEVSLRD HLRAGASEEE
360 370 380 390 400
LLRIIGAAVG RKKRQHAGMF NIAQMKNRPM ILIGVLLMLQ DSPPARWSNF
410 420 430 440 450
SWDPLRVRNP SARQCLSDQM ASLWKRHCIP KALPLSQQCL GSGSPQRHYS
460 470 480 490 500
SYPDPDTHSK CLSTGSQAPD APSGPGPTSN QLTHVDSAGR ASMVDVGGKP
510 520 530 540 550
ETERVAVASA MVLLGPVAFK LVQQNQLKKG DALVVAQLAG VQAAKLTSQL
560 570 580 590 600
IPLCHHVALS HVQVHLELDS TRHAVLIQAS CRARGPTGVE MEALTSAAMA
610 620 630
ALTVYDMCKA VSRDIVVTEV KLISKTGGQR GDFHRA
Length:636
Mass (Da):69,859
Last modified:April 14, 2009 - v2
Checksum:i9AEFB16970B3EE9F
GO
Isoform Mocs1a (identifier: Q5RKZ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     385-385: V → G
     386-636: Missing.

Show »
Length:385
Mass (Da):42,980
Checksum:i5A2486FF1AF54912
GO
Isoform 3 (identifier: Q5RKZ7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-387: Missing.

Show »
Length:249
Mass (Da):26,628
Checksum:iF714394D01C4D122
GO

Sequence cautioni

The sequence AAI47413 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti267M → V in AAF67845 (PubMed:10917590).Curated1
Sequence conflicti274R → Q in AAH49914 (PubMed:15489334).Curated1
Sequence conflicti289A → T in AAF67845 (PubMed:10917590).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0368491 – 387Missing in isoform 3. 1 PublicationAdd BLAST387
Alternative sequenceiVSP_036850385V → G in isoform Mocs1a. 2 Publications1
Alternative sequenceiVSP_036851386 – 636Missing in isoform Mocs1a. 2 PublicationsAdd BLAST251

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC165258 Genomic DNA. No translation available.
BC027444 mRNA. Translation: AAH27444.1.
BC049914 mRNA. Translation: AAH49914.1.
BC147411 mRNA. Translation: AAI47412.1.
BC147412 mRNA. Translation: AAI47413.1. Different initiation.
BC172067 mRNA. Translation: AAI72067.1.
AF214016 mRNA. Translation: AAF67845.1.
AF214016 mRNA. Translation: AAF67846.1.
CCDSiCCDS50143.1. [Q5RKZ7-1]
CCDS57100.1. [Q5RKZ7-2]
RefSeqiNP_064426.2. NM_020042.2. [Q5RKZ7-1]
NP_082740.1. NM_028464.1. [Q5RKZ7-2]
UniGeneiMm.22256.

Genome annotation databases

EnsembliENSMUST00000024797; ENSMUSP00000024797; ENSMUSG00000064120. [Q5RKZ7-2]
ENSMUST00000173033; ENSMUSP00000133694; ENSMUSG00000064120. [Q5RKZ7-1]
GeneIDi56738.
KEGGimmu:56738.
UCSCiuc008cyg.2. mouse. [Q5RKZ7-1]
uc008cyh.2. mouse. [Q5RKZ7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC165258 Genomic DNA. No translation available.
BC027444 mRNA. Translation: AAH27444.1.
BC049914 mRNA. Translation: AAH49914.1.
BC147411 mRNA. Translation: AAI47412.1.
BC147412 mRNA. Translation: AAI47413.1. Different initiation.
BC172067 mRNA. Translation: AAI72067.1.
AF214016 mRNA. Translation: AAF67845.1.
AF214016 mRNA. Translation: AAF67846.1.
CCDSiCCDS50143.1. [Q5RKZ7-1]
CCDS57100.1. [Q5RKZ7-2]
RefSeqiNP_064426.2. NM_020042.2. [Q5RKZ7-1]
NP_082740.1. NM_028464.1. [Q5RKZ7-2]
UniGeneiMm.22256.

3D structure databases

ProteinModelPortaliQ5RKZ7.
SMRiQ5RKZ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000133694.

PTM databases

iPTMnetiQ5RKZ7.
PhosphoSitePlusiQ5RKZ7.

Proteomic databases

PaxDbiQ5RKZ7.
PeptideAtlasiQ5RKZ7.
PRIDEiQ5RKZ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024797; ENSMUSP00000024797; ENSMUSG00000064120. [Q5RKZ7-2]
ENSMUST00000173033; ENSMUSP00000133694; ENSMUSG00000064120. [Q5RKZ7-1]
GeneIDi56738.
KEGGimmu:56738.
UCSCiuc008cyg.2. mouse. [Q5RKZ7-1]
uc008cyh.2. mouse. [Q5RKZ7-2]

Organism-specific databases

CTDi4337.
MGIiMGI:1928904. Mocs1.

Phylogenomic databases

eggNOGiKOG2876. Eukaryota.
COG0315. LUCA.
COG2896. LUCA.
GeneTreeiENSGT00390000016567.
HOGENOMiHOG000228683.
InParanoidiQ5RKZ7.
KOiK03639.
OMAiDMCKAVT.
OrthoDBiEOG091G0HQS.
PhylomeDBiQ5RKZ7.
TreeFamiTF300424.

Enzyme and pathway databases

UniPathwayiUPA00344.
ReactomeiR-MMU-947581. Molybdenum cofactor biosynthesis.

Miscellaneous databases

PROiQ5RKZ7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000064120.
ExpressionAtlasiQ5RKZ7. baseline and differential.
GenevisibleiQ5RKZ7. MM.

Family and domain databases

CDDicd01420. MoaC_PE. 1 hit.
Gene3Di3.20.20.70. 1 hit.
3.30.70.640. 1 hit.
HAMAPiMF_01225_B. MoaA_B. 1 hit.
MF_01224_B. MoaC_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR023045. Mo_CF_biosynth-C.
IPR023046. Mo_CF_biosynth-C_bac.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR002820. Mopterin_CF_biosynth-C_dom.
IPR007197. rSAM.
[Graphical view]
PfamiPF01967. MoaC. 1 hit.
PF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
SUPFAMiSSF55040. SSF55040. 1 hit.
TIGRFAMsiTIGR02666. moaA. 1 hit.
TIGR00581. moaC. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMOCS1_MOUSE
AccessioniPrimary (citable) accession number: Q5RKZ7
Secondary accession number(s): B2RVW8
, B7ZWI5, Q8R058, Q9JL32, Q9JL33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 14, 2009
Last modified: November 30, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

The C-terminus of Mocs1a was previously believed to be thiocarboxylated, but it is now known not to be the case.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.