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Protein

DNA polymerase lambda

Gene

Poll

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei310 – 3101Schiff-base intermediate with DNABy similarity
Metal bindingi425 – 4251ManganeseBy similarity
Metal bindingi427 – 4271ManganeseBy similarity
Metal bindingi488 – 4881ManganeseBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Lyase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication, DNA synthesis

Keywords - Ligandi

DNA-binding, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-5693571. Nonhomologous End-Joining (NHEJ).

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase lambda (EC:2.7.7.7, EC:4.2.99.-)
Short name:
Pol Lambda
Gene namesi
Name:Poll
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1308053. Poll.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 573573DNA polymerase lambdaPRO_0000218786Add
BLAST

Proteomic databases

PaxDbiQ5RKI3.
PRIDEiQ5RKI3.

PTM databases

iPTMnetiQ5RKI3.

Expressioni

Gene expression databases

GenevisibleiQ5RKI3. RN.

Interactioni

Subunit structurei

Binds PCNA.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023137.

Structurei

3D structure databases

ProteinModelPortaliQ5RKI3.
SMRiQ5RKI3. Positions 35-134, 247-573.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 13197BRCTPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni263 – 27715DNA bindingBy similarityAdd
BLAST
Regioni343 – 3464DNA bindingBy similarity
Regioni418 – 42710Involved in primer bindingBy similarity
Regioni464 – 50340DNA bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-X family.Curated
Contains 1 BRCT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2534. Eukaryota.
COG1796. LUCA.
GeneTreeiENSGT00530000063002.
HOGENOMiHOG000007787.
HOVERGENiHBG002788.
InParanoidiQ5RKI3.
KOiK03512.
OMAiEGMDCER.
OrthoDBiEOG73Z2SX.
PhylomeDBiQ5RKI3.
TreeFamiTF103011.

Family and domain databases

Gene3Di1.10.150.110. 1 hit.
3.30.210.10. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR002054. DNA-dir_DNA_pol_X.
IPR019843. DNA_pol-X_BS.
IPR010996. DNA_pol_b-like_N.
IPR028207. DNA_pol_B_palm_palm.
IPR018944. DNA_pol_lambd_fingers_domain.
IPR022312. DNA_pol_X.
IPR002008. DNA_pol_X_beta-like.
IPR027421. DNA_pol_X_lyase_dom.
IPR029398. PolB_thumb.
[Graphical view]
PfamiPF14792. DNA_pol_B_palm. 1 hit.
PF14791. DNA_pol_B_thumb. 1 hit.
PF10391. DNA_pol_lambd_f. 1 hit.
PF14716. HHH_8. 1 hit.
[Graphical view]
PRINTSiPR00869. DNAPOLX.
PR00870. DNAPOLXBETA.
SMARTiSM00483. POLXc. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00522. DNA_POLYMERASE_X. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5RKI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPQGILKAF PKRKKIHADP SSNALAKIPK REAGDARGWL SSLRAHIMPT
60 70 80 90 100
GIGRARAELF EKQIIQHGGQ VCSAQAPGVT HIVVDEGMDY ERALRLLRLP
110 120 130 140 150
QLPPGAQLVK SAWLSLCLQE KKLTDTDGFS LSSPKRSLNE PQPSKSGQDA
160 170 180 190 200
SAPGTQGVLP RTTLSLSPPC TRAVSPPPKA EKPPKTQTQL SSEDEASDGE
210 220 230 240 250
GPQVSSADLQ ALISGHYPTP PGEDGGPDPA PEALGKWVCA QPSSQKATNY
260 270 280 290 300
NLHITEKLEV LAKAYNVQGD KWRALGYAKA INALKSFHKP VSSYQEACSI
310 320 330 340 350
PGVGRRMAEK VMEILESGHL RKLDHISDSV PVLELFSNIW GAGTKTAQMW
360 370 380 390 400
YHQGFRSLED IRGLASLTAQ QAIGLKHYDD FLDRMPREEA AEIEQMVRVS
410 420 430 440 450
AQAFNPGLLC VACGSFRRGK VTCGDVDVLI THPDGRSHQG IFSPLLDSLR
460 470 480 490 500
QQGFLTDDLV SQEENGQQQK YLGVCRLPGA GQRHRRLDII VVPYSEFACA
510 520 530 540 550
LLYFTGSAHF NRSMRALAKT KGMSLSEHAL SAAVVRNSQG VKVGAGQVLP
560 570
TPTEKDVFKL LGLPYREPAE RDW
Length:573
Mass (Da):62,401
Last modified:December 21, 2004 - v1
Checksum:i5978076D6CC463B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085841 mRNA. Translation: AAH85841.1.
RefSeqiNP_001014190.1. NM_001014168.1.
UniGeneiRn.11799.

Genome annotation databases

EnsembliENSRNOT00000023137; ENSRNOP00000023137; ENSRNOG00000016748.
GeneIDi361767.
KEGGirno:361767.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085841 mRNA. Translation: AAH85841.1.
RefSeqiNP_001014190.1. NM_001014168.1.
UniGeneiRn.11799.

3D structure databases

ProteinModelPortaliQ5RKI3.
SMRiQ5RKI3. Positions 35-134, 247-573.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023137.

PTM databases

iPTMnetiQ5RKI3.

Proteomic databases

PaxDbiQ5RKI3.
PRIDEiQ5RKI3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023137; ENSRNOP00000023137; ENSRNOG00000016748.
GeneIDi361767.
KEGGirno:361767.

Organism-specific databases

CTDi27343.
RGDi1308053. Poll.

Phylogenomic databases

eggNOGiKOG2534. Eukaryota.
COG1796. LUCA.
GeneTreeiENSGT00530000063002.
HOGENOMiHOG000007787.
HOVERGENiHBG002788.
InParanoidiQ5RKI3.
KOiK03512.
OMAiEGMDCER.
OrthoDBiEOG73Z2SX.
PhylomeDBiQ5RKI3.
TreeFamiTF103011.

Enzyme and pathway databases

ReactomeiR-RNO-5693571. Nonhomologous End-Joining (NHEJ).

Miscellaneous databases

PROiQ5RKI3.

Gene expression databases

GenevisibleiQ5RKI3. RN.

Family and domain databases

Gene3Di1.10.150.110. 1 hit.
3.30.210.10. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR002054. DNA-dir_DNA_pol_X.
IPR019843. DNA_pol-X_BS.
IPR010996. DNA_pol_b-like_N.
IPR028207. DNA_pol_B_palm_palm.
IPR018944. DNA_pol_lambd_fingers_domain.
IPR022312. DNA_pol_X.
IPR002008. DNA_pol_X_beta-like.
IPR027421. DNA_pol_X_lyase_dom.
IPR029398. PolB_thumb.
[Graphical view]
PfamiPF14792. DNA_pol_B_palm. 1 hit.
PF14791. DNA_pol_B_thumb. 1 hit.
PF10391. DNA_pol_lambd_f. 1 hit.
PF14716. HHH_8. 1 hit.
[Graphical view]
PRINTSiPR00869. DNAPOLX.
PR00870. DNAPOLXBETA.
SMARTiSM00483. POLXc. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00522. DNA_POLYMERASE_X. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiDPOLL_RAT
AccessioniPrimary (citable) accession number: Q5RKI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: December 21, 2004
Last modified: June 8, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.