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Protein

Protein OS-9

Gene

Os9

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei130 – 1301CarbohydrateBy similarity
Binding sitei182 – 1821CarbohydrateBy similarity
Binding sitei188 – 1881CarbohydrateBy similarity
Binding sitei212 – 2121CarbohydrateBy similarity
Binding sitei218 – 2181CarbohydrateBy similarity

GO - Molecular functioni

  • carbohydrate binding Source: UniProtKB-KW
  • glycoprotein binding Source: UniProtKB
  • protease binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Lectin

Enzyme and pathway databases

ReactomeiR-RNO-5358346. Hedgehog ligand biogenesis.
R-RNO-901032. ER Quality Control Compartment (ERQC).

Names & Taxonomyi

Protein namesi
Recommended name:
Protein OS-9
Gene namesi
Name:Os9
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1359574. Os9.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: RGD
  • endoplasmic reticulum lumen Source: UniProtKB
  • Hrd1p ubiquitin ligase complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence analysisAdd
BLAST
Chaini31 – 666636Protein OS-9PRO_0000386450Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi110 ↔ 123By similarity
Glycosylationi177 – 1771N-linked (GlcNAc...)Sequence analysis
Disulfide bondi181 ↔ 216By similarity
Disulfide bondi196 ↔ 228By similarity

Post-translational modificationi

Intramolecular disulfide bonds.By similarity
N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ5RKH6.
PRIDEiQ5RKH6.

Expressioni

Gene expression databases

GenevisibleiQ5RKH6. RN.

Interactioni

Subunit structurei

Probably part of the HRD1 ubiquitin ligase complex composed at least of SYVN1/HRD1 and SEL1L with which it interacts directly. Through this complex it may interact with ERLEC1 and HSPA5. Interacts with DERL2. Interacts with HSP90B1, CREB3 and SYVN1. Interacts (via C-terminus) with CPNE6 (via second C2 domain); this interaction occurs in a calcium-dependent manner in vitro.By similarity

GO - Molecular functioni

  • glycoprotein binding Source: UniProtKB
  • protease binding Source: RGD

Protein-protein interaction databases

BioGridi263807. 2 interactions.
STRINGi10116.ENSRNOP00000044914.

Structurei

3D structure databases

ProteinModelPortaliQ5RKH6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini108 – 17871PRKCSHAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi410 – 42819Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Belongs to the OS-9 family.Curated
Contains 1 PRKCSH domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3394. Eukaryota.
ENOG410XR8A. LUCA.
GeneTreeiENSGT00530000063603.
HOGENOMiHOG000293348.
InParanoidiQ5RKH6.
KOiK10088.
OMAiFQNNVQV.
OrthoDBiEOG7R8315.
PhylomeDBiQ5RKH6.
TreeFamiTF314309.

Family and domain databases

Gene3Di2.70.130.10. 1 hit.
InterProiIPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR012913. PRKCSH.
[Graphical view]
PfamiPF07915. PRKCSH. 1 hit.
[Graphical view]
SUPFAMiSSF50911. SSF50911. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5RKH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAEALLSSL LGLLFLGLLL PAHLTGGVGS LNLEELSEMR YGIQILPLPV
60 70 80 90 100
MGGQSQASDV VVVSSKYKQR YECRLPAGAI HFQREREEET PAYQGPGIPE
110 120 130 140 150
LLSPMRDAPC LLKTKDWWTY EFCYGRHIQQ YHMEDSEIKG DVLYLGYYQS
160 170 180 190 200
AFNWDDETAK ASKQHRLKRY HSQTYGNGSK CDLNGKPREA EVRFLCDEGA
210 220 230 240 250
GISGDYIDRV DEPFSCSYVL SIRTSRLCPH PLLRPPASAA PQAILCHPAL
260 270 280 290 300
QPDEYMAYLQ RQAESKQHEE KVTEEVQDTD HQVWSGSKAA GAPPKKEDVS
310 320 330 340 350
PTKEDKESEF WKMLQEPEEQ ATGTEEAQAG EQDLNHEAAA DPAPAPPTDF
360 370 380 390 400
QNNVQVKLIR SPADLIRLIE ELKGAEKGKP SVRQEQPGDD TTEAPQREAE
410 420 430 440 450
AKGKGGEPRG LVEEEDGDEE EEDEDEDEQQ LLGEFEKELE GMLLPSDRER
460 470 480 490 500
LRSEVKAGME RELENIIQET EKELDPEGLR KESEREQAIL ALTSTLDKLI
510 520 530 540 550
KRLQESQSPE LVQKYKKRRV VPQKPPPSPH PTEEEPEHRV RVRVTKLRHG
560 570 580 590 600
GPNQDLTVLE MNRENPQLKQ IEGLVTEVLE REGLTAEGKI EIKIVRPGAE
610 620 630 640 650
GKEEDTRWLT DEDTRNLKEI FFNILVQGAE EANKERQRQS ELESNYRRVW
660
GSPGGEDTGD LDEFDF
Length:666
Mass (Da):75,365
Last modified:December 21, 2004 - v1
Checksum:i8E1F48228C671ED6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085907 mRNA. Translation: AAH85907.1.
RefSeqiNP_001007266.1. NM_001007265.1.
UniGeneiRn.1579.

Genome annotation databases

EnsembliENSRNOT00000049895; ENSRNOP00000044914; ENSRNOG00000025570.
GeneIDi362891.
KEGGirno:362891.
UCSCiRGD:1359574. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085907 mRNA. Translation: AAH85907.1.
RefSeqiNP_001007266.1. NM_001007265.1.
UniGeneiRn.1579.

3D structure databases

ProteinModelPortaliQ5RKH6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi263807. 2 interactions.
STRINGi10116.ENSRNOP00000044914.

Proteomic databases

PaxDbiQ5RKH6.
PRIDEiQ5RKH6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000049895; ENSRNOP00000044914; ENSRNOG00000025570.
GeneIDi362891.
KEGGirno:362891.
UCSCiRGD:1359574. rat.

Organism-specific databases

CTDi10956.
RGDi1359574. Os9.

Phylogenomic databases

eggNOGiKOG3394. Eukaryota.
ENOG410XR8A. LUCA.
GeneTreeiENSGT00530000063603.
HOGENOMiHOG000293348.
InParanoidiQ5RKH6.
KOiK10088.
OMAiFQNNVQV.
OrthoDBiEOG7R8315.
PhylomeDBiQ5RKH6.
TreeFamiTF314309.

Enzyme and pathway databases

ReactomeiR-RNO-5358346. Hedgehog ligand biogenesis.
R-RNO-901032. ER Quality Control Compartment (ERQC).

Miscellaneous databases

PROiQ5RKH6.

Gene expression databases

GenevisibleiQ5RKH6. RN.

Family and domain databases

Gene3Di2.70.130.10. 1 hit.
InterProiIPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR012913. PRKCSH.
[Graphical view]
PfamiPF07915. PRKCSH. 1 hit.
[Graphical view]
SUPFAMiSSF50911. SSF50911. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  2. "Mammalian OS-9 is upregulated in response to endoplasmic reticulum stress and facilitates ubiquitination of misfolded glycoproteins."
    Alcock F., Swanton E.
    J. Mol. Biol. 385:1032-1042(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.

Entry informationi

Entry nameiOS9_RAT
AccessioniPrimary (citable) accession number: Q5RKH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: December 21, 2004
Last modified: June 8, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.