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Protein

Solute carrier family 12 member 7

Gene

Slc12a7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K+ uptake into Deiters' cells in the cochlea and contribute to K+ recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl- extrusion in the kidney and contribute to renal acidification (By similarity).By similarity

Enzyme regulationi

Inhibited by WNK3.By similarity

GO - Molecular functioni

  • potassium:chloride symporter activity Source: RGD
  • potassium ion symporter activity Source: GO_Central

GO - Biological processi

  • cell volume homeostasis Source: RGD
  • chemical synaptic transmission Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Ion transport, Potassium transport, Symport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiR-RNO-426117. Cation-coupled Chloride cotransporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 12 member 7
Alternative name(s):
Electroneutral potassium-chloride cotransporter 4
K-Cl cotransporter 4
Gene namesi
Name:Slc12a7Imported
Synonyms:Kcc4Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1359672. Slc12a7.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 118CytoplasmicSequence analysisAdd BLAST118
Transmembranei119 – 139HelicalSequence analysisAdd BLAST21
Transmembranei141 – 161HelicalSequence analysisAdd BLAST21
Topological domaini162 – 200CytoplasmicSequence analysisAdd BLAST39
Transmembranei201 – 221HelicalSequence analysisAdd BLAST21
Transmembranei253 – 273HelicalSequence analysisAdd BLAST21
Topological domaini274 – 275CytoplasmicSequence analysis2
Transmembranei276 – 296HelicalSequence analysisAdd BLAST21
Transmembranei416 – 436HelicalSequence analysisAdd BLAST21
Topological domaini437 – 455CytoplasmicSequence analysisAdd BLAST19
Transmembranei456 – 476HelicalSequence analysisAdd BLAST21
Transmembranei494 – 514HelicalSequence analysisAdd BLAST21
Topological domaini515 – 553CytoplasmicSequence analysisAdd BLAST39
Transmembranei554 – 574HelicalSequence analysisAdd BLAST21
Transmembranei577 – 597HelicalSequence analysisAdd BLAST21
Topological domaini598 – 624CytoplasmicSequence analysisAdd BLAST27
Transmembranei625 – 645HelicalSequence analysisAdd BLAST21
Transmembranei845 – 865HelicalSequence analysisAdd BLAST21
Topological domaini866 – 1083CytoplasmicSequence analysisAdd BLAST218

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002990761 – 1083Solute carrier family 12 member 7Add BLAST1083

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei30PhosphoserineBy similarity1
Modified residuei33PhosphoserineBy similarity1
Modified residuei50PhosphoserineBy similarity1
Modified residuei62PhosphoserineBy similarity1
Glycosylationi312N-linked (GlcNAc...)Sequence analysis1
Glycosylationi331N-linked (GlcNAc...)Sequence analysis1
Glycosylationi360N-linked (GlcNAc...)Sequence analysis1
Modified residuei973PhosphothreonineBy similarity1
Modified residuei980PhosphothreonineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ5RK27.
PRIDEiQ5RK27.

PTM databases

iPTMnetiQ5RK27.
PhosphoSitePlusiQ5RK27.

Expressioni

Tissue specificityi

Widely expressed with highest levels in kidney, liver and pancreas. Expressed in choroid plexus and suprachiasmatic nucleus.1 Publication

Gene expression databases

BgeeiENSRNOG00000016372.
ExpressionAtlasiQ5RK27. baseline and differential.
GenevisibleiQ5RK27. RN.

Interactioni

Subunit structurei

Homomultimer and heteromultimer with other K-Cl cotransporters.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022635.

Structurei

3D structure databases

ProteinModelPortaliQ5RK27.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SLC12A transporter family.Sequence analysis

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2082. Eukaryota.
COG0531. LUCA.
GeneTreeiENSGT00760000119053.
HOGENOMiHOG000092644.
HOVERGENiHBG052852.
InParanoidiQ5RK27.
KOiK13627.
PhylomeDBiQ5RK27.
TreeFamiTF313657.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR030354. KCC4.
IPR000076. KCL_cotranspt.
IPR018491. SLC12_C.
IPR004842. SLC12A_fam.
[Graphical view]
PANTHERiPTHR11827:SF47. PTHR11827:SF47. 4 hits.
PfamiPF00324. AA_permease. 2 hits.
PF03522. SLC12. 2 hits.
[Graphical view]
PRINTSiPR01081. KCLTRNSPORT.
TIGRFAMsiTIGR00930. 2a30. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5RK27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTNFTVVPV EARADGAGDE AAERTEEPGS PESADPACPT PGDGNPRENS
60 70 80 90 100
PFINNVEVER ESYFEGKNMA LFEEEMDSNP MVSSLLNKLA NYTNLSQGVV
110 120 130 140 150
EHEEDEDSRR REIKAPRMGT FIGVYLPCLQ NILGVILFLR LTWIVGAAGV
160 170 180 190 200
LESFLIVAMC CTCTMLTAIS MSAIATNGVV PAGGSYYMIS RSLGPEFGGA
210 220 230 240 250
VGLCFYLGTT FAGAMYILGT IEIFLTYISP SAAIFQAETA DGEAAALLNN
260 270 280 290 300
MRVYGSCALA LMAVVVFVGV KYVNKLALVF LACVVLSILA IYAGVIKTAF
310 320 330 340 350
APPDIPVCLL GNRTLANRNF DTCAKMQVVS NGTVTTALWR LFCNGSSLGA
360 370 380 390 400
SCDEYFVQNN VTEIQGIPGV ASGVFLDNLW STYSDKGAFV EKKGVSSVPV
410 420 430 440 450
SEESRPGGLP YVLTDIMTYF TMLVGIYFPS VTGIMAGSNR SGDLKDAQKS
460 470 480 490 500
IPTGTILAIV TTSFIYLSCI VLFGACIEGV VLRDKFGEAL QGNLVIGMLA
510 520 530 540 550
WPSPWVIVIG SFFSTCGAGL QSLTGAPRLL QAIARDGIIP FLQVFGHGKA
560 570 580 590 600
NGEPTWALLL TALICETGIL IASLDSVAPI LSMFFLMCYM FVNLACAVQT
610 620 630 640 650
LLRTPNWRPR FKFYHWTLSF LGMSLCLALM FICSWYYALF AMLIAGCIYK
660 670 680 690 700
YIEYRGAEKE WGDGIRGLSL NAARYALLRV EHGPPHTKNW RPQVLVMLNL
710 720 730 740 750
DSEQCVKHPR LLSFTSQLKA GKGLTIVGSV LEGTYLDKHV EAQRAEENIR
760 770 780 790 800
SLMSAEKMKG FCQLVVSSNL RDGASHLIQS AGLGGMKHNT VLMAWPEAWK
810 820 830 840 850
QADNPFSWKN FVDTVRDTTA AHQALLVAKN IDLFPQNQER FSDGNIDVWW
860 870 880 890 900
IVHDGGMLML LPFLLRQHKV WRKCRMRIFT VAQVDDNSIQ MKKDLQMFLY
910 920 930 940 950
HLRISAEVEV VEMVENDISA FTYEKTLMME QRSQMLKQMQ LSKNEREREA
960 970 980 990 1000
QLIHDRNTAS HTVATARTEA PPTPDKVQMT WTKEKLIAEK HRNKDTGTSG
1010 1020 1030 1040 1050
FKDLFSLKPD QSNVRRMHTA VKLNGVVLNK SQDAQLVLLN MPGPPKSRQG
1060 1070 1080
DENYMEFLEV LTEGLNRVLL VRGGGREVIT IYS
Length:1,083
Mass (Da):119,378
Last modified:August 21, 2007 - v2
Checksum:i111C2D9EE0A16BA5
GO

Sequence cautioni

The sequence AAH86339 differs from that shown. Intron retention. This sequence is incomplete at its 3'end and extensively differs from that shown in positions 214-251.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti125Y → F in AAQ93481 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03000133 mRNA. No translation available.
BC086339 mRNA. Translation: AAH86339.1. Sequence problems.
AY429042 mRNA. Translation: AAR10805.1.
AY387486 mRNA. Translation: AAQ93480.1.
AY387487 mRNA. Translation: AAQ93481.1.
RefSeqiNP_001013162.2. NM_001013144.2.
UniGeneiRn.64199.

Genome annotation databases

EnsembliENSRNOT00000022635; ENSRNOP00000022635; ENSRNOG00000016372.
GeneIDi308069.
KEGGirno:308069.
UCSCiRGD:1359672. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03000133 mRNA. No translation available.
BC086339 mRNA. Translation: AAH86339.1. Sequence problems.
AY429042 mRNA. Translation: AAR10805.1.
AY387486 mRNA. Translation: AAQ93480.1.
AY387487 mRNA. Translation: AAQ93481.1.
RefSeqiNP_001013162.2. NM_001013144.2.
UniGeneiRn.64199.

3D structure databases

ProteinModelPortaliQ5RK27.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022635.

PTM databases

iPTMnetiQ5RK27.
PhosphoSitePlusiQ5RK27.

Proteomic databases

PaxDbiQ5RK27.
PRIDEiQ5RK27.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022635; ENSRNOP00000022635; ENSRNOG00000016372.
GeneIDi308069.
KEGGirno:308069.
UCSCiRGD:1359672. rat.

Organism-specific databases

CTDi10723.
RGDi1359672. Slc12a7.

Phylogenomic databases

eggNOGiKOG2082. Eukaryota.
COG0531. LUCA.
GeneTreeiENSGT00760000119053.
HOGENOMiHOG000092644.
HOVERGENiHBG052852.
InParanoidiQ5RK27.
KOiK13627.
PhylomeDBiQ5RK27.
TreeFamiTF313657.

Enzyme and pathway databases

ReactomeiR-RNO-426117. Cation-coupled Chloride cotransporters.

Miscellaneous databases

PROiQ5RK27.

Gene expression databases

BgeeiENSRNOG00000016372.
ExpressionAtlasiQ5RK27. baseline and differential.
GenevisibleiQ5RK27. RN.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR030354. KCC4.
IPR000076. KCL_cotranspt.
IPR018491. SLC12_C.
IPR004842. SLC12A_fam.
[Graphical view]
PANTHERiPTHR11827:SF47. PTHR11827:SF47. 4 hits.
PfamiPF00324. AA_permease. 2 hits.
PF03522. SLC12. 2 hits.
[Graphical view]
PRINTSiPR01081. KCLTRNSPORT.
TIGRFAMsiTIGR00930. 2a30. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiS12A7_RAT
AccessioniPrimary (citable) accession number: Q5RK27
Secondary accession number(s): Q6T7Y5, Q6TUA1, Q6TUA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: August 21, 2007
Last modified: November 2, 2016
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.