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Protein

Vacuolar-sorting protein SNF8

Gene

Snf8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization (By similarity). Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL.Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

ReactomeiR-RNO-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar-sorting protein SNF8
Alternative name(s):
ELL-associated protein of 30 kDa
ESCRT-II complex subunit VPS22
Gene namesi
Name:Snf8
Synonyms:Eap30
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1310144 Snf8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002152111 – 258Vacuolar-sorting protein SNF8Add BLAST258

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4Omega-N-methylarginineBy similarity1

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiQ5RK19
PRIDEiQ5RK19

Expressioni

Gene expression databases

BgeeiENSRNOG00000006500
GenevisibleiQ5RK19 RN

Interactioni

Subunit structurei

Interacts with TSG101 (via the C-terminal domain). Interacts with RILPL1 (via the N-terminal domain); which recruits ESCRT-II to the endosome membranes. Interacts with 14-3-3 proteins (By similarity). Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS25 and VPS36 (PubMed:11278625). SNF8 is essential for the stability of the ESCRT-II complex. ESCRT-II interacts with ELL (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008964

Structurei

3D structure databases

ProteinModelPortaliQ5RK19
SMRiQ5RK19
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili27 – 53Sequence analysisAdd BLAST27

Sequence similaritiesi

Belongs to the SNF8 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3341 Eukaryota
ENOG410XPVM LUCA
GeneTreeiENSGT00390000007843
HOGENOMiHOG000170930
HOVERGENiHBG079872
InParanoidiQ5RK19
KOiK12188
OMAiQYWLPAL
OrthoDBiEOG091G0GWO
PhylomeDBiQ5RK19
TreeFamiTF105950

Family and domain databases

Gene3Di1.10.10.10, 2 hits
InterProiView protein in InterPro
IPR016689 ESCRT-2_cplx_Snf8
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12806 PTHR12806, 1 hit
PIRSFiPIRSF017215 ESCRT2_Vps22, 1 hit
SUPFAMiSSF46785 SSF46785, 2 hits

Sequencei

Sequence statusi: Complete.

Q5RK19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRRGVGAGA IAKKKLAEAK YKERGTVLAE DQLAQMSKQL DMFKTNLEEF
60 70 80 90 100
ASKHKQEIRK NPEFRVQFQD MCATIGVDPL ASGKGFWSEM LGVGDFYYEL
110 120 130 140 150
GVQIIEVCLA LKHRNGGLIT LEELHQQVLK GRGKFAQDVS QDDLIRAIKK
160 170 180 190 200
LKALGTGFGI IPVGGTYLIQ SVPAELNMDH TVVLQLAEKN GYVTVSEIKA
210 220 230 240 250
SLKWETERAR QVLEHLLKEG LAWLDLQAPG EAHYWLPALF TDLYSQEISA

EEAKEVFP
Length:258
Mass (Da):28,884
Last modified:December 21, 2004 - v1
Checksum:iFD649E7BD794485E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC086364 mRNA Translation: AAH86364.1
RefSeqiNP_001007805.1, NM_001007804.1
UniGeneiRn.162167

Genome annotation databases

EnsembliENSRNOT00000008964; ENSRNOP00000008964; ENSRNOG00000006500
GeneIDi287645
KEGGirno:287645
UCSCiRGD:1310144 rat

Similar proteinsi

Entry informationi

Entry nameiSNF8_RAT
AccessioniPrimary (citable) accession number: Q5RK19
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: December 21, 2004
Last modified: March 28, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health