Q5RK19 (SNF8_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar-sorting protein SNF8

Alternative name(s):
ELL-associated protein of 30 kDa
ESCRT-II complex subunit VPS22

Gene names

Name:	Snf8
Synonyms:	Eap30

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization By similarity. Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Ref.2
Subunit structure	Interacts with TSG101 (via the C-terminal domain). Interacts with RILPL1 (via the N-terminal domain); which recruits ESCRT-II to the endosome membranes. Interacts with 14-3-3 proteins By similarity. Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS25 and VPS36. SNF8 is essential for the stability of the ESCRT-II complex. ESCRT-II interacts with ELL.
Subcellular location	Cytoplasm By similarity. Endosome membrane By similarity. Nucleus Probable. Late endosome membrane By similarity. Note: Recruited to the endosome membrane to participate in vesicle formation By similarity.
Sequence similarities	Belongs to the SNF8 family.

Ontologies

Keywords
Biological process	Protein transport Transcription Transcription regulation Transport
Cellular component	Cytoplasm Endosome Membrane Nucleus
Domain	Coiled coil
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	late endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Vacuolar-sorting protein SNF8		PRO_0000215211
Regions
	Coiled coil	27 – 53	27	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5RK19 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: FD649E7BD794485E	FASTA	258	28,884
	10 20 30 40 50 60 MHRRGVGAGA IAKKKLAEAK YKERGTVLAE DQLAQMSKQL DMFKTNLEEF ASKHKQEIRK 70 80 90 100 110 120 NPEFRVQFQD MCATIGVDPL ASGKGFWSEM LGVGDFYYEL GVQIIEVCLA LKHRNGGLIT 130 140 150 160 170 180 LEELHQQVLK GRGKFAQDVS QDDLIRAIKK LKALGTGFGI IPVGGTYLIQ SVPAELNMDH 190 200 210 220 230 240 TVVLQLAEKN GYVTVSEIKA SLKWETERAR QVLEHLLKEG LAWLDLQAPG EAHYWLPALF 250 TDLYSQEISA EEAKEVFP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[2]	"Cloning and characterization of the EAP30 subunit of the ELL complex that confers derepression of transcription by RNA polymerase II." Schmidt A.E., Miller T., Schmidt S.L., Shiekhattar R., Shilatifard A. J. Biol. Chem. 274:21981-21985(1999) [PubMed: 10419521] [Abstract] Cited for: PROTEIN SEQUENCE OF 37-53; 135-146 AND 211-218, FUNCTION IN TRANSCRIPTION. Tissue: Liver.
[3]	"Cloning and characterization of ELL-associated proteins EAP45 and EAP20. a role for yeast EAP-like proteins in regulation of gene expression by glucose." Kamura T., Burian D., Khalili H., Schmidt S.L., Sato S., Liu W.-J., Conrad M.N., Conaway R.C., Conaway J.W., Shilatifard A. J. Biol. Chem. 276:16528-16533(2001) [PubMed: 11278625] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH ELL; VPS25 AND VPS36.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC086364 mRNA. Translation: AAH86364.1.
IPI	IPI00192521.
RefSeq	NP_001007805.1. NM_001007804.1.
UniGene	Rn.162167.
3D structure databases
ProteinModelPortal	Q5RK19.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5RK19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000008964; ENSRNOP00000008964; ENSRNOG00000006500.
GeneID	287645.
KEGG	rno:287645.
NMPDR	fig|10116.3.peg.5946.
UCSC	BC086364. rat.
Organism-specific databases
CTD	11267.
RGD	1310144. Snf8.
Phylogenomic databases
eggNOG	roNOG08252.
GeneTree	ENSGT00390000007843.
HOVERGEN	HBG079872.
InParanoid	Q5RK19.
OMA	QFQEMCA.
OrthoDB	EOG4V6ZHD.
PhylomeDB	Q5RK19.
Gene expression databases
ArrayExpress	Q5RK19.
Genevestigator	Q5RK19.
GermOnline	ENSRNOG00000006500. Rattus norvegicus.
Family and domain databases
InterPro	IPR007286. EAP30. IPR016689. ESCRT-2_cplx_Snf8. [Graphical view]
KO	K12188.
PANTHER	PTHR12806. EAP30. 1 hit.
Pfam	PF04157. EAP30. 1 hit. [Graphical view]
PIRSF	PIRSF017215. ESCRT2_Vps22. 1 hit.
ProtoNet	Search...
Other
NextBio	626715.

Entry information

Entry name

SNF8_RAT

Accession

Primary (citable) accession number: Q5RK19

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	December 21, 2004
Last modified:	November 16, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program