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Protein

Regulator of telomere elongation helicase 1

Gene

Rtel1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

ATP-dependent DNA helicase implicated in telomere-length regulation, DNA repair and the maintenance of genomic stability. Acts as an anti-recombinase to counteract toxic recombination and limit crossover during meiosis. Regulates meiotic recombination and crossover homeostasis by physically dissociating strand invasion events and thereby promotes noncrossover repair by meiotic synthesis dependent strand annealing (SDSA) as well as disassembly of D loop recombination intermediates. Also disassembles T loops and prevents telomere fragility by counteracting telomeric G4-DNA structures, which together ensure the dynamics and stability of the telomere.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi145 – 1451Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi163 – 1631Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi172 – 1721Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi207 – 2071Iron-sulfur (4Fe-4S)UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi42 – 498ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

4Fe-4S, ATP-binding, DNA-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-5693554. Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA).

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of telomere elongation helicase 1UniRule annotation (EC:3.6.4.12UniRule annotation)
Gene namesi
Name:Rtel1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1306721. Rtel1.

Subcellular locationi

  • Nucleus UniRule annotation

  • Note: Colocalizes with PCNA within the replication foci in S-phase cells.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12741274Regulator of telomere elongation helicase 1PRO_0000370613Add
BLAST

Proteomic databases

PaxDbiQ5RJZ1.
PRIDEiQ5RJZ1.

PTM databases

iPTMnetiQ5RJZ1.

Expressioni

Gene expression databases

ExpressionAtlasiQ5RJZ1. baseline and differential.
GenevisibleiQ5RJZ1. RN.

Interactioni

Subunit structurei

Interacts with TERF1. Interacts (via PIP-box) with PCNA; the interaction is direct and essential for suppressing telomere fragility. Interacts with MMS19; the interaction mediates the association of RTEL1 with the cytosolic iron-sulfur protein assembly (CIA) complex.UniRule annotation

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000051909.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 296290Helicase ATP-bindingUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi151 – 16717Nuclear localization signalUniRule annotationAdd
BLAST
Motifi250 – 2534DEAH box
Motifi871 – 8777Nuclear localization signalUniRule annotation

Domaini

The PIP-box (PCNA interacting peptide) motif mediates the interaction with PCNA and localization to replication foci.UniRule annotation

Sequence similaritiesi

Belongs to the helicase family. RAD3/XPD subfamily.UniRule annotation
Contains 1 helicase ATP-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiKOG1132. Eukaryota.
COG1199. LUCA.
GeneTreeiENSGT00530000063199.
HOGENOMiHOG000007558.
InParanoidiQ5RJZ1.
KOiK11136.
OMAiFALEMQI.
OrthoDBiEOG7N8ZTR.
PhylomeDBiQ5RJZ1.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
HAMAPiMF_03065. RTEL1.
InterProiIPR006555. ATP-dep_Helicase_C.
IPR010614. DEAD_2.
IPR013020. DNA_helicase_DNA-repair_Rad3.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR006554. Helicase-like_DEXD_c2.
IPR027417. P-loop_NTPase.
IPR030845. RTEL1.
[Graphical view]
PfamiPF06733. DEAD_2. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view]
SMARTiSM00488. DEXDc2. 1 hit.
SM00491. HELICc2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 8 hits.
TIGRFAMsiTIGR00604. rad3. 1 hit.
PROSITEiPS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5RJZ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPRVVLNGVT VDFPFQPYPC QQEYMTKVLE CLQKKVNGIL ESPTGTGKTL
60 70 80 90 100
CLLCTTLAWR EHLRDAVSSL KIAERVQGEL FASRTLSSWR SAADANGDSI
110 120 130 140 150
DCYTDIPKII YASRTHSQLT QVIGELRNTS YRPKVCVLGS REQLCIHPEV
160 170 180 190 200
KKQESNHMQI SLCRKKVASR SCHFYNNVEE KSLEQELATP ILDIEDLVKN
210 220 230 240 250
GSKHKVCPYY LSRNLKQQAD IIFMPYNYLL DAKSRKAHNI DLKGTVVIFD
260 270 280 290 300
EAHNVEKICE ESASFDLTPR DVASGLEVIN QVLEEQARVA QHGELQQEFI
310 320 330 340 350
IDTSSSGLNM ELEDIAKLKM ILLHLEEAID AVQLPGDDRG VTKPGSYIFE
360 370 380 390 400
LFAEAQITFQ TKGCILESLD QIIQHLTGRT GVFTNTAGLQ KLMDIIQIVF
410 420 430 440 450
SVDPLEGSPG SQVGLGSSHF YKVHIHPETS HRRAAQRSDA WSTTASRKQG
460 470 480 490 500
KVLSYWCFSP SHSMRELVQQ GVRTLILTSG TLAPLSSFAL EMQIPFPVCL
510 520 530 540 550
ENPHIIDKNQ LWVGVIPRGP DGVQLSSAYD KRFSEECLSS LGKALGNIAR
560 570 580 590 600
VVPHGLLVFF PSYPVMEKSL EFWQAQGMSK KVEALKPLFV EPRNKGSFSE
610 620 630 640 650
VIDAYYQQVA SPGSNGATFL AVCRGKASEG LDFSDMNGRG VIVTGLPYPP
660 670 680 690 700
RMDPRVILKM QFLDEMKGRS RVGGQCLSGQ EWYQQQASRA VNQAIGRVIR
710 720 730 740 750
HRHDYGAIFL CDHRFAYADA RAHLPSWVRP YLKVYDNFGR VIRDVAQFFR
760 770 780 790 800
VAQKAMPLPV PQAVTSSVSE GEAAVKEATL SSHSLSTRKA MSLDVHVPSL
810 820 830 840 850
RRRPVGLPTA GDSESSVCVE YEQQTFSAQK RPMGLLAALE YNEQKAGASE
860 870 880 890 900
EQALSSSTPS LRCEKRLSVE QRGGKKKVRL VNHPEEPVAG TQAGRAKMFM
910 920 930 940 950
VAVKQALSQA NFDTFTQALQ HYKSSDDFEA LVASLTCLFA EDPKKHTLLK
960 970 980 990 1000
GFYQFVRPHH KQQFEDICFQ LTGQRCSYQP GNSLPFGEQA QSTASKQGRR
1010 1020 1030 1040 1050
ELESKLTLSE GADRQLDPGE HLNQGWPHLS THLTSKGDTS NCPKVGCVGE
1060 1070 1080 1090 1100
KPGQPAVNDY LSDVHKALGS ASCNQLTAAL RAYKQDDDLD KVLAVVAALT
1110 1120 1130 1140 1150
TAKPEHLSLL QRFGMFIRRH HKPRFVQTCA DLMGLPTIGK GLELPCPRDE
1160 1170 1180 1190 1200
STTVPSELTH EDMKPGPSTS KKPEKTQSKI SSFLRQRPDQ SARSDDTIMQ
1210 1220 1230 1240 1250
LPPRLPPEHT TSQWNFVCPA CATEDTVLFQ CPSCDFCRCR ACWQRQLQAS
1260 1270
RLCPACGAVN RKQSIAQVIW PKPQ
Length:1,274
Mass (Da):141,779
Last modified:May 5, 2009 - v2
Checksum:iCF7D27F451824347
GO
Isoform 2 (identifier: Q5RJZ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     995-998: SKQG → R
     1112-1139: RFGMFIRRHHKPRFVQTCADLMGLPTIG → S

Note: No experimental confirmation available.
Show »
Length:1,244
Mass (Da):138,354
Checksum:iE41BE37ABA61D10B
GO

Sequence cautioni

The sequence EDL88727.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei995 – 9984SKQG → R in isoform 2. 1 PublicationVSP_036956
Alternative sequencei1112 – 113928RFGMF…LPTIG → S in isoform 2. 1 PublicationVSP_036957Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474066 Genomic DNA. Translation: EDL88727.1. Sequence problems.
BC086436 mRNA. Translation: AAH86436.1.
RefSeqiNP_001178786.1. NM_001191857.1. [Q5RJZ1-1]
UniGeneiRn.98315.

Genome annotation databases

EnsembliENSRNOT00000055030; ENSRNOP00000051909; ENSRNOG00000027513. [Q5RJZ1-1]
GeneIDi362288.
KEGGirno:362288.
UCSCiRGD:1306721. rat. [Q5RJZ1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474066 Genomic DNA. Translation: EDL88727.1. Sequence problems.
BC086436 mRNA. Translation: AAH86436.1.
RefSeqiNP_001178786.1. NM_001191857.1. [Q5RJZ1-1]
UniGeneiRn.98315.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000051909.

PTM databases

iPTMnetiQ5RJZ1.

Proteomic databases

PaxDbiQ5RJZ1.
PRIDEiQ5RJZ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000055030; ENSRNOP00000051909; ENSRNOG00000027513. [Q5RJZ1-1]
GeneIDi362288.
KEGGirno:362288.
UCSCiRGD:1306721. rat. [Q5RJZ1-1]

Organism-specific databases

CTDi51750.
RGDi1306721. Rtel1.

Phylogenomic databases

eggNOGiKOG1132. Eukaryota.
COG1199. LUCA.
GeneTreeiENSGT00530000063199.
HOGENOMiHOG000007558.
InParanoidiQ5RJZ1.
KOiK11136.
OMAiFALEMQI.
OrthoDBiEOG7N8ZTR.
PhylomeDBiQ5RJZ1.

Enzyme and pathway databases

ReactomeiR-RNO-5693554. Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA).

Miscellaneous databases

PROiQ5RJZ1.

Gene expression databases

ExpressionAtlasiQ5RJZ1. baseline and differential.
GenevisibleiQ5RJZ1. RN.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
HAMAPiMF_03065. RTEL1.
InterProiIPR006555. ATP-dep_Helicase_C.
IPR010614. DEAD_2.
IPR013020. DNA_helicase_DNA-repair_Rad3.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR006554. Helicase-like_DEXD_c2.
IPR027417. P-loop_NTPase.
IPR030845. RTEL1.
[Graphical view]
PfamiPF06733. DEAD_2. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view]
SMARTiSM00488. DEXDc2. 1 hit.
SM00491. HELICc2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 8 hits.
TIGRFAMsiTIGR00604. rad3. 1 hit.
PROSITEiPS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 868-1274 (ISOFORM 2).
    Tissue: Ovary.

Entry informationi

Entry nameiRTEL1_RAT
AccessioniPrimary (citable) accession number: Q5RJZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: June 8, 2016
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.