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Protein

pre-rRNA processing protein FTSJ3

Gene

Ftsj3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable methyltransferase involved in the processing of the 34S pre-rRNA to 18S rRNA and in 40S ribosomal subunit formation.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing 2'-O-methylnucleoside.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei56 – 561S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei58 – 581S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei76 – 761S-adenosyl-L-methionineUniRule annotation
Binding sitei92 – 921S-adenosyl-L-methionineUniRule annotation
Binding sitei117 – 1171S-adenosyl-L-methionineUniRule annotation
Active sitei157 – 1571Proton acceptorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
pre-rRNA processing protein FTSJ3UniRule annotation (EC:2.1.1.-UniRule annotation)
Alternative name(s):
2'-O-ribose RNA methyltransferase SPB1 homologUniRule annotation
Protein ftsJ homolog 3UniRule annotation
Putative rRNA methyltransferase 3UniRule annotation
Gene namesi
Name:Ftsj3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1307110. Ftsj3.

Subcellular locationi

  • Nucleusnucleolus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 829829pre-rRNA processing protein FTSJ3PRO_0000155580Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei333 – 3331PhosphoserineBy similarity
Modified residuei335 – 3351PhosphoserineCombined sources
Modified residuei336 – 3361PhosphoserineCombined sources
Modified residuei345 – 3451PhosphoserineCombined sources
Modified residuei353 – 3531PhosphoserineCombined sources
Modified residuei389 – 3891CitrullineBy similarity
Modified residuei531 – 5311PhosphoserineCombined sources
Modified residuei544 – 5441PhosphoserineBy similarity
Modified residuei575 – 5751PhosphoserineBy similarity
Modified residuei659 – 6591PhosphoserineBy similarity
Modified residuei671 – 6711PhosphoserineBy similarity
Modified residuei766 – 7661CitrullineBy similarity

Post-translational modificationi

Citrullinated by PADI4.By similarity

Keywords - PTMi

Citrullination, Phosphoprotein

Proteomic databases

PaxDbiQ5RJT2.
PRIDEiQ5RJT2.

PTM databases

iPTMnetiQ5RJT2.

Expressioni

Gene expression databases

GenevisibleiQ5RJT2. RN.

Interactioni

Subunit structurei

Interacts with NIP7.UniRule annotation

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013295.

Structurei

3D structure databases

ProteinModelPortaliQ5RJT2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili722 – 76039UniRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi337 – 37943Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. SPB1 subfamily.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1098. Eukaryota.
COG0293. LUCA.
GeneTreeiENSGT00550000075004.
HOGENOMiHOG000176256.
HOVERGENiHBG075246.
InParanoidiQ5RJT2.
KOiK14857.
OMAiIANHKKT.
OrthoDBiEOG7WX07S.
PhylomeDBiQ5RJT2.
TreeFamiTF106102.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01547. RNA_methyltr_E.
MF_03163. RNA_methyltr_E_SPB1.
InterProiIPR015507. rRNA-MeTfrase_E.
IPR012920. rRNA_MeTfrase_Spb1_C.
IPR024576. rRNA_MeTfrase_Spb1_DUF3381.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
IPR028589. Spb1.
[Graphical view]
PANTHERiPTHR10920. PTHR10920. 3 hits.
PTHR10920:SF13. PTHR10920:SF13. 3 hits.
PfamiPF11861. DUF3381. 1 hit.
PF01728. FtsJ. 1 hit.
PF07780. Spb1_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5RJT2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKGKVGKS RRDKFYHLAK ETGYRSRSAF KLIQLNRRFQ FLQKARALLD
60 70 80 90 100
LCAAPGGWLQ VAAKFMPVSS LIVGVDLVPI KPLPNVVTLQ EDITTERCRQ
110 120 130 140 150
ALRKELKTWK VDVVLNDGAP NVGASWVHDA YSQAHLTLMA LRLACDFLAR
160 170 180 190 200
GGCFITKVFR SRDYQPLLWI FQQLFHRVQA TKPQASRHES AEIFVVCQGF
210 220 230 240 250
LAPDKVDAKF FDPKFAFKEV EVQAKTVTEL VTKKKPKAEG YAEGDLTLYH
260 270 280 290 300
RTSVTDFLRA ANPVDFLSKA SEISIDDKEL AQHPATTEDI RACCQDIKVL
310 320 330 340 350
GRKELRSLLN WRTKLRRYVA KKLKEQAKAL DISLSSEEEG DEEESAAETK
360 370 380 390 400
QASEEEEERE EEEQLNRTLA EMKAQEVAEL KRKKKKLLRE QRKQRERVEL
410 420 430 440 450
KMDLPGVSIA DEGETGMFSL RTIRGQQLLE EVTQGDMNAA DTFLSDLPRD
460 470 480 490 500
DIYVSDAEDD DDTSLESDLD PEELAGVRTH SDQKEQKSLQ FAQVDDSKEE
510 520 530 540 550
EGENPLLVPL EEKAVLQEEQ ASLWFSKDGF SGIDDDADEA LEISQAQLLY
560 570 580 590 600
KSRQKEQQPT DPPPPPTNLK TEKKSSQCQN EVPKETEAIT DTGGEDRDSS
610 620 630 640 650
DSDSSSSEDE DDWKVSRGKK RSRGSKADED GFEVVPIEDP VKYRILDPEG
660 670 680 690 700
LALGAVIASS KKAKRDLIDN SFNRYAFNEE EEELPEWFVQ EEKQHRIRQL
710 720 730 740 750
PLDKKEVEHY RKRWREINAR PIKKVAEAKA RKKRRMLKKL EQTKKKAEAV
760 770 780 790 800
VNTVDISERE KVAQLRSLYK KAGLGKEKRQ VTYVVAKKGV GRKVRRPAGV
810 820
RGHFKVVDSR MKKDQRAQRK EQKRNHRRK
Length:829
Mass (Da):94,767
Last modified:December 21, 2004 - v1
Checksum:i00CF7A6D13B1B40F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC086512 mRNA. Translation: AAH86512.1.
RefSeqiNP_001012014.1. NM_001012014.1.
UniGeneiRn.47509.

Genome annotation databases

EnsembliENSRNOT00000013295; ENSRNOP00000013295; ENSRNOG00000009857.
GeneIDi303608.
KEGGirno:303608.
UCSCiRGD:1307110. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC086512 mRNA. Translation: AAH86512.1.
RefSeqiNP_001012014.1. NM_001012014.1.
UniGeneiRn.47509.

3D structure databases

ProteinModelPortaliQ5RJT2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013295.

PTM databases

iPTMnetiQ5RJT2.

Proteomic databases

PaxDbiQ5RJT2.
PRIDEiQ5RJT2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013295; ENSRNOP00000013295; ENSRNOG00000009857.
GeneIDi303608.
KEGGirno:303608.
UCSCiRGD:1307110. rat.

Organism-specific databases

CTDi117246.
RGDi1307110. Ftsj3.

Phylogenomic databases

eggNOGiKOG1098. Eukaryota.
COG0293. LUCA.
GeneTreeiENSGT00550000075004.
HOGENOMiHOG000176256.
HOVERGENiHBG075246.
InParanoidiQ5RJT2.
KOiK14857.
OMAiIANHKKT.
OrthoDBiEOG7WX07S.
PhylomeDBiQ5RJT2.
TreeFamiTF106102.

Miscellaneous databases

PROiQ5RJT2.

Gene expression databases

GenevisibleiQ5RJT2. RN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01547. RNA_methyltr_E.
MF_03163. RNA_methyltr_E_SPB1.
InterProiIPR015507. rRNA-MeTfrase_E.
IPR012920. rRNA_MeTfrase_Spb1_C.
IPR024576. rRNA_MeTfrase_Spb1_DUF3381.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
IPR028589. Spb1.
[Graphical view]
PANTHERiPTHR10920. PTHR10920. 3 hits.
PTHR10920:SF13. PTHR10920:SF13. 3 hits.
PfamiPF11861. DUF3381. 1 hit.
PF01728. FtsJ. 1 hit.
PF07780. Spb1_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-335; SER-336; SER-345; SER-353 AND SER-531, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSPB1_RAT
AccessioniPrimary (citable) accession number: Q5RJT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: December 21, 2004
Last modified: July 6, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.