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Protein

Protein-tyrosine sulfotransferase 2

Gene

Tpst2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides.By similarity

Catalytic activityi

3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei77 – 771By similarity
Active sitei98 – 981By similarity
Sitei157 – 1571Transition state stabilizerBy similarity
Sitei284 – 2841Transition state stabilizerBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-tyrosine sulfotransferase 2 (EC:2.8.2.20)
Alternative name(s):
Tyrosylprotein sulfotransferase 2
Short name:
TPST-2
Gene namesi
Name:Tpst2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi1305331. Tpst2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence analysis
Transmembranei9 – 2517Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini26 – 376351LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Protein-tyrosine sulfotransferase 2PRO_0000253725Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi95 ↔ 155By similarity
Disulfide bondi224 ↔ 232By similarity
Glycosylationi342 – 3421N-linked (GlcNAc...)Sequence analysis
Glycosylationi367 – 3671N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ5RJS8.
PRIDEiQ5RJS8.

PTM databases

PhosphoSiteiQ5RJS8.

Expressioni

Gene expression databases

BgeeiENSRNOG00000000664.
GenevisibleiQ5RJS8. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000831.

Structurei

3D structure databases

ProteinModelPortaliQ5RJS8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protein sulfotransferase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3988. Eukaryota.
ENOG410XQ2X. LUCA.
GeneTreeiENSGT00390000006030.
HOGENOMiHOG000253020.
HOVERGENiHBG001270.
InParanoidiQ5RJS8.
OMAiGKDKCLP.
OrthoDBiEOG091G09YJ.
PhylomeDBiQ5RJS8.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR026634. TPST.
[Graphical view]
PANTHERiPTHR12788. PTHR12788. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Q5RJS8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLSVRKVLL AVGCALALVL AVQLGQQVLE CRAVLGGVRS PRRMQPEQEE
60 70 80 90 100
LVMLGADHVE YRYGKTMPLI FVGGVPRSGT TLMRAMLDAH PEVRCGEETR
110 120 130 140 150
IIPRVLAMRQ AWTKSGREKL RLDEAGVTDE VLDAAMQAFI LEVIAKHGEP
160 170 180 190 200
ARVLCNKDPF TLKSSVYLAR LFPNSKFLLM VRDGRASVHS MITRKVTIAG
210 220 230 240 250
FDLSSYRDCL TKWNKAIEVM YAQCMEVGRD KCLPVYYEQL VLHPRRSLKR
260 270 280 290 300
ILDFLGIAWS DTVLHHEDLI GKPGGVSLSK IERSTDQVIK PVNLEALSKW
310 320 330 340 350
TGHIPRDVVR DMAQIAPMLA RLGYDPYANP PNYGNPDPIV INNTHRVLKG
360 370
DYKTPANLKG YFQVNQNSTS PHLGSS
Length:376
Mass (Da):42,068
Last modified:December 21, 2004 - v1
Checksum:iC0FE61AF3B46B114
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC086518 mRNA. Translation: AAH86518.1.
UniGeneiRn.3659.

Genome annotation databases

EnsembliENSRNOT00000000831; ENSRNOP00000000831; ENSRNOG00000000664.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC086518 mRNA. Translation: AAH86518.1.
UniGeneiRn.3659.

3D structure databases

ProteinModelPortaliQ5RJS8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000831.

PTM databases

PhosphoSiteiQ5RJS8.

Proteomic databases

PaxDbiQ5RJS8.
PRIDEiQ5RJS8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000831; ENSRNOP00000000831; ENSRNOG00000000664.

Organism-specific databases

RGDi1305331. Tpst2.

Phylogenomic databases

eggNOGiKOG3988. Eukaryota.
ENOG410XQ2X. LUCA.
GeneTreeiENSGT00390000006030.
HOGENOMiHOG000253020.
HOVERGENiHBG001270.
InParanoidiQ5RJS8.
OMAiGKDKCLP.
OrthoDBiEOG091G09YJ.
PhylomeDBiQ5RJS8.

Miscellaneous databases

PROiQ5RJS8.

Gene expression databases

BgeeiENSRNOG00000000664.
GenevisibleiQ5RJS8. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR026634. TPST.
[Graphical view]
PANTHERiPTHR12788. PTHR12788. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiTPST2_RAT
AccessioniPrimary (citable) accession number: Q5RJS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: December 21, 2004
Last modified: September 7, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Substrate peptides must be flexible in order to adopt an L-shaped conformation in the deep binding cleft.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.