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Protein

Twinfilin-1

Gene

Twf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Twinfilin-1
Gene namesi
Name:Twf1
Synonyms:Ptk9
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1311872 Twf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002324062 – 350Twinfilin-1Add BLAST349

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei143PhosphoserineCombined sources1
Modified residuei277PhosphoserineCombined sources1
Modified residuei309PhosphotyrosineBy similarity1
Modified residuei349PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated on serine and threonine residues.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5RJR2
PRIDEiQ5RJR2

PTM databases

iPTMnetiQ5RJR2
PhosphoSitePlusiQ5RJR2

Expressioni

Gene expression databases

BgeeiENSRNOG00000022507
GenevisibleiQ5RJR2 RN

Interactioni

Subunit structurei

Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated. Interacts with ACTG1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000030138

Structurei

3D structure databases

ProteinModelPortaliQ5RJR2
SMRiQ5RJR2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 139ADF-H 1PROSITE-ProRule annotationAdd BLAST138
Domaini175 – 313ADF-H 2PROSITE-ProRule annotationAdd BLAST139

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1747 Eukaryota
ENOG410XS1J LUCA
GeneTreeiENSGT00530000063868
HOGENOMiHOG000168296
HOVERGENiHBG000848
InParanoidiQ5RJR2
KOiK08870
OMAiYDYLQFR
OrthoDBiEOG091G0BWK
PhylomeDBiQ5RJR2
TreeFamiTF352598

Family and domain databases

Gene3Di3.40.20.10, 2 hits
InterProiView protein in InterPro
IPR002108 ADF-H
IPR029006 ADF-H/Gelsolin-like_dom_sf
IPR028458 Twinfilin
PANTHERiPTHR13759 PTHR13759, 1 hit
PfamiView protein in Pfam
PF00241 Cofilin_ADF, 2 hits
SMARTiView protein in SMART
SM00102 ADF, 2 hits
PROSITEiView protein in PROSITE
PS51263 ADF_H, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5RJR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHQTGIQAS EDVKEIFARA RNGKYRLLKI SIENEQLVVG SCSPPSDSWE
60 70 80 90 100
QDYDPFVLPL LEDKQPCYVL FRLDSQNAQG YEWIFIAWSP DHSHVRQKML
110 120 130 140 150
YAATRATLKK EFGGGHIKDE VFGTVKEDVS LHGYRKYLLS QSSPAPLTAA
160 170 180 190 200
EEELRQIKIS EVQTDVSVDT KHQTLQGVAF PISRDAFQAL EKLSKRQLNY
210 220 230 240 250
VQLEIDIKNE TIILANTENT ELKDLPKRIP KDSARYHFFL YKHSHEGDYL
260 270 280 290 300
ESIVFIYSMP GYTCSIRERM LYSSCKSPLL DIVERQLQMD VIRKIEIDNG
310 320 330 340 350
DELTADFLYD EVHPKQHAHK QSFAKPKGPA GKRGIRRLIR GPAEAEATTD
Length:350
Mass (Da):40,090
Last modified:December 21, 2004 - v1
Checksum:i99706DAA174B8913
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC086536 mRNA Translation: AAH86536.1
RefSeqiNP_001008521.1, NM_001008521.1
UniGeneiRn.162971

Genome annotation databases

EnsembliENSRNOT00000038032; ENSRNOP00000030138; ENSRNOG00000022507
GeneIDi315265
KEGGirno:315265

Similar proteinsi

Entry informationi

Entry nameiTWF1_RAT
AccessioniPrimary (citable) accession number: Q5RJR2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: December 21, 2004
Last modified: April 25, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health