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Q5RJR2 (TWF1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Twinfilin-1
Gene names
Name:Twf1
Synonyms:Ptk9
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles By similarity.

Subunit structure

Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated By similarity.

Subcellular location

Cytoplasm. Cytoplasm › cytoskeleton By similarity. Note: Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts By similarity.

Post-translational modification

Phosphorylated on serine and threonine residues By similarity.

Sequence similarities

Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.

Contains 2 ADF-H domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 350349Twinfilin-1
PRO_0000232406

Regions

Domain2 – 139138ADF-H 1
Domain175 – 313139ADF-H 2

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue1431Phosphoserine By similarity
Modified residue3091Phosphotyrosine By similarity
Modified residue3491Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5RJR2 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: 99706DAA174B8913

FASTA35040,090
        10         20         30         40         50         60 
MSHQTGIQAS EDVKEIFARA RNGKYRLLKI SIENEQLVVG SCSPPSDSWE QDYDPFVLPL 

        70         80         90        100        110        120 
LEDKQPCYVL FRLDSQNAQG YEWIFIAWSP DHSHVRQKML YAATRATLKK EFGGGHIKDE 

       130        140        150        160        170        180 
VFGTVKEDVS LHGYRKYLLS QSSPAPLTAA EEELRQIKIS EVQTDVSVDT KHQTLQGVAF 

       190        200        210        220        230        240 
PISRDAFQAL EKLSKRQLNY VQLEIDIKNE TIILANTENT ELKDLPKRIP KDSARYHFFL 

       250        260        270        280        290        300 
YKHSHEGDYL ESIVFIYSMP GYTCSIRERM LYSSCKSPLL DIVERQLQMD VIRKIEIDNG 

       310        320        330        340        350 
DELTADFLYD EVHPKQHAHK QSFAKPKGPA GKRGIRRLIR GPAEAEATTD

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.

Web resources

Protein Spotlight

Molecular embrace - Issue 73 of August 2006

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC086536 mRNA. Translation: AAH86536.1.
IPIIPI00361693.
RefSeqNP_001008521.1. NM_001008521.1.
UniGeneRn.162971.

3D structure databases

ProteinModelPortalQ5RJR2.
SMRQ5RJR2. Positions 7-139, 161-313.
ModBaseSearch...

PTM databases

PhosphoSiteQ5RJR2.

Proteomic databases

PaxDbQ5RJR2.
PRIDEQ5RJR2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000038032; ENSRNOP00000030138; ENSRNOG00000022507.
GeneID315265.
KEGGrno:315265.

Organism-specific databases

CTD5756.
RGD1311872. Twf1.

Phylogenomic databases

eggNOGNOG263290.
GeneTreeENSGT00530000063868.
HOGENOMHOG000168296.
HOVERGENHBG000848.
InParanoidQ5RJR2.
KOK08870.
OMAVIRKIEI.
OrthoDBEOG4TTGJJ.

Gene expression databases

GenevestigatorQ5RJR2.
GermOnlineENSRNOG00000022507. Rattus norvegicus.

Family and domain databases

InterProIPR002108. Actin-bd_cofilin/tropomyosin.
[Graphical view]
PfamPF00241. Cofilin_ADF. 2 hits.
[Graphical view]
SMARTSM00102. ADF. 2 hits.
[Graphical view]
PROSITEPS51263. ADF_H. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio668975.

Entry information

Entry nameTWF1_RAT
AccessionPrimary (citable) accession number: Q5RJR2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: December 21, 2004
Last modified: April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

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