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Q5RJQ4 (SIR2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD-dependent protein deacetylase sirtuin-2

EC=3.5.1.-
Alternative name(s):
Regulatory protein SIR2 homolog 2
SIR2-like protein 2
Gene names
Name:Sirt2
Synonyms:Sir2l2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and non-histone proteins such as ACLY, PCK1 and RELA. Deacetylates 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Deacetylates PCK1, opposing proteasomal degradation. Deacetylates 'Lys-310' of RELA By similarity.

Catalytic activity

NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Enzyme regulation

Inhibited by Sirtinol, A3 and M15 small molecules. Inhibited by nicotinamide By similarity.

Subunit structure

Interacts with HDAC6, suggesting that these proteins belong to a large complex that deacetylate the cytoskeleton By similarity.

Subcellular location

Cytoplasmcytoskeleton By similarity. Note: Colocalizes with microtubules By similarity.

Induction

In oligodendrocytes during differentiation of CG-4 cells. Ref.3

Post-translational modification

Phosphorylated at the G2/M transition of the cell cycle By similarity.

Miscellaneous

Has some ability to deacetylate histones in vitro, but seeing its subcellular location, this is unlikely in vivo By similarity.

Sequence similarities

Belongs to the sirtuin family. Class I subfamily.

Contains 1 deacetylase sirtuin-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 350350NAD-dependent protein deacetylase sirtuin-2
PRO_0000244536

Regions

Domain28 – 303276Deacetylase sirtuin-type
Nucleotide binding47 – 6721NAD By similarity
Nucleotide binding130 – 1334NAD By similarity
Nucleotide binding224 – 2263NAD By similarity
Nucleotide binding249 – 2513NAD By similarity

Sites

Active site1501Proton acceptor By similarity
Metal binding1581Zinc By similarity
Metal binding1631Zinc By similarity
Metal binding1841Zinc By similarity
Metal binding1871Zinc By similarity
Binding site2871NAD; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5RJQ4 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: EA3621A7CCE695FB

FASTA35039,319
        10         20         30         40         50         60 
MDFLRNLFTQ TLGLGSQKER LLDELTLEGV TRYMQSERCR RVICLVGAGI STSAGIPDFR 

        70         80         90        100        110        120 
SPSTGLYANL EKYHLPYPEA IFEISYFKKH PEPFFALAKE LYPGQFKPTI CHYFIRLLKE 

       130        140        150        160        170        180 
KGLLLRCYTQ NIDTLERVAG LEPQDLVEAH GTFYTSHCVN TSCGKEYTMS WMKEKIFSEA 

       190        200        210        220        230        240 
TPKCEKCQNV VKPDIVFFGE NLPPRFFSCM QSDFSKVDLL IIMGTSLQVQ PFASLISKAP 

       250        260        270        280        290        300 
LATPRLLINK EKTGQTDPFL GMMMGLGGGM DFDSKKAYRD VAWLGDCDQG CLALADLLGW 

       310        320        330        340        350 
KELEDLVRRE HANIDAQSGS QASNPSATVS PRKSPPPAKE AARTKEKEEH 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[2]Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U.
Submitted (JUL-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 6-18; 21-32; 41-116; 176-183; 187-216; 239-245; 302-308 AND 310-332, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain, Hippocampus and Spinal cord.
[3]"A novel seven transmembrane receptor induced during the early steps of astrocyte differentiation identified by differential expression."
De Smet C., Nishimori H., Furnari F.B., Boegler O., Huang H.-J.S., Cavenee W.K.
J. Neurochem. 81:575-588(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC086545 mRNA. Translation: AAH86545.1.
RefSeqNP_001008369.1. NM_001008368.1.
UniGeneRn.59887.

3D structure databases

ProteinModelPortalQ5RJQ4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid262752. 1 interaction.
STRING10116.ENSRNOP00000027384.

PTM databases

PhosphoSiteQ5RJQ4.

Proteomic databases

PaxDbQ5RJQ4.
PRIDEQ5RJQ4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000064153; ENSRNOP00000059450; ENSRNOG00000020102.
GeneID361532.
KEGGrno:361532.
UCSCRGD:621481. rat.

Organism-specific databases

CTD22933.
RGD621481. Sirt2.

Phylogenomic databases

eggNOGCOG0846.
GeneTreeENSGT00740000115546.
HOGENOMHOG000085952.
HOVERGENHBG057095.
InParanoidQ5RJQ4.
KOK11412.
OMATICHYFM.
OrthoDBEOG7WX09C.

Gene expression databases

GenevestigatorQ5RJQ4.

Family and domain databases

InterProIPR003000. Sirtuin.
IPR017328. Sirtuin_class_I.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERPTHR11085. PTHR11085. 1 hit.
PfamPF02146. SIR2. 1 hit.
[Graphical view]
PIRSFPIRSF037938. SIR2_euk. 1 hit.
PROSITEPS50305. SIRTUIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio676633.

Entry information

Entry nameSIR2_RAT
AccessionPrimary (citable) accession number: Q5RJQ4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: December 21, 2004
Last modified: April 16, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families