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Q5RJQ0

- CHST1_RAT

UniProt

Q5RJQ0 - CHST1_RAT

Protein

Carbohydrate sulfotransferase 1

Gene

Chst1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (21 Dec 2004)
      Previous versions | rss
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    Functioni

    Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation By similarity.By similarity

    Catalytic activityi

    3'-phosphoadenylyl sulfate + keratan = adenosine 3',5'-bisphosphate + keratan 6'-sulfate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi69 – 757PAPSBy similarity
    Nucleotide bindingi234 – 2429PAPSBy similarity

    GO - Molecular functioni

    1. keratan sulfotransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. galactose metabolic process Source: Ensembl
    2. inflammatory response Source: UniProtKB-KW
    3. keratan sulfate metabolic process Source: Ensembl

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Inflammatory response

    Enzyme and pathway databases

    ReactomeiREACT_196475. Keratan sulfate biosynthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbohydrate sulfotransferase 1 (EC:2.8.2.21)
    Alternative name(s):
    Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1
    Short name:
    GST-1
    Keratan sulfate Gal-6 sulfotransferase
    Short name:
    KS6ST
    Short name:
    KSGal6ST
    Short name:
    KSST
    Gene namesi
    Name:Chst1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi1308142. Chst1.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 411411Carbohydrate sulfotransferase 1PRO_0000085184Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi189 – 1891N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi334 – 3341N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ5RJQ0.

    Expressioni

    Gene expression databases

    GenevestigatoriQ5RJQ0.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000010510.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5RJQ0.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 11CytoplasmicSequence Analysis
    Topological domaini24 – 411388LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei2 – 2322Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi337 – 3393Cell attachment siteSequence Analysis

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG80862.
    GeneTreeiENSGT00530000062902.
    HOGENOMiHOG000261614.
    HOVERGENiHBG106811.
    InParanoidiQ5RJQ0.
    KOiK01022.
    OMAiCEEGPTF.
    OrthoDBiEOG7RZ5S0.
    PhylomeDBiQ5RJQ0.
    TreeFamiTF342871.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR016469. Carbohydrate_sulfotransferase.
    IPR027417. P-loop_NTPase.
    IPR000863. Sulfotransferase_dom.
    [Graphical view]
    PfamiPF00685. Sulfotransfer_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
    SUPFAMiSSF52540. SSF52540. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q5RJQ0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQCSWKAVLL LALASIAIQY TAIRTFTAKS FHTCPGLTET GLAERLCEEG    50
    PTFSYNLSRK THVLILATTR SGSSFVGQLF NQHMDVFYLF EPLYHVQNTL 100
    IPRFTQGKSP ADRRVMLGAS RDLLRSLYDC DLYFLENYIK PPPVNHTTDR 150
    VFRRGASRVL CSRPVCDPPG ASDLILEEGD CVRKCGLLNL TLAAEACRER 200
    SHVAIKTVRV PEVNDLRALV EDPRLNLKVI QLVRDPRGIL ASRSETFRDT 250
    YRLWRLWYGT GRKPYNLDVT QLTTVCEDFS SSVSTGLMRP SWLKGKYMLV 300
    RYEDLARNPM KKTEEIYEFL GIPLDSHVAR WIQNNTRGDP TLGKHKYGTV 350
    RNSAATAEKW RFRLSYDIVA FAQNACQHVL AQLGYKMATS EEELKNPAIS 400
    LVEERDFRPF L 411
    Length:411
    Mass (Da):46,885
    Last modified:December 21, 2004 - v1
    Checksum:i715998736106E5CD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC086551 mRNA. Translation: AAH86551.1.
    RefSeqiNP_001011955.1. NM_001011955.1.
    XP_006234611.1. XM_006234549.1.
    UniGeneiRn.16695.

    Genome annotation databases

    EnsembliENSRNOT00000010510; ENSRNOP00000010510; ENSRNOG00000007989.
    GeneIDi295934.
    KEGGirno:295934.
    UCSCiRGD:1308142. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC086551 mRNA. Translation: AAH86551.1 .
    RefSeqi NP_001011955.1. NM_001011955.1.
    XP_006234611.1. XM_006234549.1.
    UniGenei Rn.16695.

    3D structure databases

    ProteinModelPortali Q5RJQ0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000010510.

    Proteomic databases

    PaxDbi Q5RJQ0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000010510 ; ENSRNOP00000010510 ; ENSRNOG00000007989 .
    GeneIDi 295934.
    KEGGi rno:295934.
    UCSCi RGD:1308142. rat.

    Organism-specific databases

    CTDi 8534.
    RGDi 1308142. Chst1.

    Phylogenomic databases

    eggNOGi NOG80862.
    GeneTreei ENSGT00530000062902.
    HOGENOMi HOG000261614.
    HOVERGENi HBG106811.
    InParanoidi Q5RJQ0.
    KOi K01022.
    OMAi CEEGPTF.
    OrthoDBi EOG7RZ5S0.
    PhylomeDBi Q5RJQ0.
    TreeFami TF342871.

    Enzyme and pathway databases

    Reactomei REACT_196475. Keratan sulfate biosynthesis.

    Miscellaneous databases

    NextBioi 640351.
    PROi Q5RJQ0.

    Gene expression databases

    Genevestigatori Q5RJQ0.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR016469. Carbohydrate_sulfotransferase.
    IPR027417. P-loop_NTPase.
    IPR000863. Sulfotransferase_dom.
    [Graphical view ]
    Pfami PF00685. Sulfotransfer_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
    SUPFAMi SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary.

    Entry informationi

    Entry nameiCHST1_RAT
    AccessioniPrimary (citable) accession number: Q5RJQ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: December 21, 2004
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3