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Q5RJQ0

- CHST1_RAT

UniProt

Q5RJQ0 - CHST1_RAT

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Protein
Carbohydrate sulfotransferase 1
Gene
Chst1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation By similarity.

Catalytic activityi

3'-phosphoadenylyl sulfate + keratan = adenosine 3',5'-bisphosphate + keratan 6'-sulfate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi69 – 757PAPS By similarity
Nucleotide bindingi234 – 2429PAPS By similarity

GO - Molecular functioni

  1. keratan sulfotransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. galactose metabolic process Source: Ensembl
  2. inflammatory response Source: UniProtKB-KW
  3. keratan sulfate metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism, Inflammatory response

Enzyme and pathway databases

ReactomeiREACT_196475. Keratan sulfate biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 1 (EC:2.8.2.21)
Alternative name(s):
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1
Short name:
GST-1
Keratan sulfate Gal-6 sulfotransferase
Short name:
KS6ST
Short name:
KSGal6ST
Short name:
KSST
Gene namesi
Name:Chst1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 3

Organism-specific databases

RGDi1308142. Chst1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 11Cytoplasmic Reviewed prediction
Transmembranei2 – 2322Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini24 – 411388Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 411411Carbohydrate sulfotransferase 1
PRO_0000085184Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi56 – 561N-linked (GlcNAc...) Reviewed prediction
Glycosylationi145 – 1451N-linked (GlcNAc...) Reviewed prediction
Glycosylationi189 – 1891N-linked (GlcNAc...) Reviewed prediction
Glycosylationi334 – 3341N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ5RJQ0.

Expressioni

Gene expression databases

GenevestigatoriQ5RJQ0.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010510.

Structurei

3D structure databases

ProteinModelPortaliQ5RJQ0.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi337 – 3393Cell attachment site Reviewed prediction

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG80862.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG106811.
InParanoidiQ5RJQ0.
KOiK01022.
OMAiCEEGPTF.
OrthoDBiEOG7RZ5S0.
PhylomeDBiQ5RJQ0.
TreeFamiTF342871.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Q5RJQ0-1 [UniParc]FASTAAdd to Basket

« Hide

MQCSWKAVLL LALASIAIQY TAIRTFTAKS FHTCPGLTET GLAERLCEEG    50
PTFSYNLSRK THVLILATTR SGSSFVGQLF NQHMDVFYLF EPLYHVQNTL 100
IPRFTQGKSP ADRRVMLGAS RDLLRSLYDC DLYFLENYIK PPPVNHTTDR 150
VFRRGASRVL CSRPVCDPPG ASDLILEEGD CVRKCGLLNL TLAAEACRER 200
SHVAIKTVRV PEVNDLRALV EDPRLNLKVI QLVRDPRGIL ASRSETFRDT 250
YRLWRLWYGT GRKPYNLDVT QLTTVCEDFS SSVSTGLMRP SWLKGKYMLV 300
RYEDLARNPM KKTEEIYEFL GIPLDSHVAR WIQNNTRGDP TLGKHKYGTV 350
RNSAATAEKW RFRLSYDIVA FAQNACQHVL AQLGYKMATS EEELKNPAIS 400
LVEERDFRPF L 411
Length:411
Mass (Da):46,885
Last modified:December 21, 2004 - v1
Checksum:i715998736106E5CD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC086551 mRNA. Translation: AAH86551.1.
RefSeqiNP_001011955.1. NM_001011955.1.
XP_006234611.1. XM_006234549.1.
UniGeneiRn.16695.

Genome annotation databases

EnsembliENSRNOT00000010510; ENSRNOP00000010510; ENSRNOG00000007989.
GeneIDi295934.
KEGGirno:295934.
UCSCiRGD:1308142. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC086551 mRNA. Translation: AAH86551.1 .
RefSeqi NP_001011955.1. NM_001011955.1.
XP_006234611.1. XM_006234549.1.
UniGenei Rn.16695.

3D structure databases

ProteinModelPortali Q5RJQ0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000010510.

Proteomic databases

PaxDbi Q5RJQ0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000010510 ; ENSRNOP00000010510 ; ENSRNOG00000007989 .
GeneIDi 295934.
KEGGi rno:295934.
UCSCi RGD:1308142. rat.

Organism-specific databases

CTDi 8534.
RGDi 1308142. Chst1.

Phylogenomic databases

eggNOGi NOG80862.
GeneTreei ENSGT00530000062902.
HOGENOMi HOG000261614.
HOVERGENi HBG106811.
InParanoidi Q5RJQ0.
KOi K01022.
OMAi CEEGPTF.
OrthoDBi EOG7RZ5S0.
PhylomeDBi Q5RJQ0.
TreeFami TF342871.

Enzyme and pathway databases

Reactomei REACT_196475. Keratan sulfate biosynthesis.

Miscellaneous databases

NextBioi 640351.
PROi Q5RJQ0.

Gene expression databases

Genevestigatori Q5RJQ0.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view ]
Pfami PF00685. Sulfotransfer_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMi SSF52540. SSF52540. 2 hits.
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiCHST1_RAT
AccessioniPrimary (citable) accession number: Q5RJQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: December 21, 2004
Last modified: September 3, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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