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Protein

Carbohydrate sulfotransferase 1

Gene

Chst1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation (By similarity).By similarity

Catalytic activityi

3'-phosphoadenylyl sulfate + keratan = adenosine 3',5'-bisphosphate + keratan 6'-sulfate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi69 – 757PAPSBy similarity
Nucleotide bindingi234 – 2429PAPSBy similarity

GO - Molecular functioni

  1. keratan sulfotransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. galactose metabolic process Source: Ensembl
  2. inflammatory response Source: UniProtKB-KW
  3. keratan sulfate metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism, Inflammatory response

Enzyme and pathway databases

ReactomeiREACT_196475. Keratan sulfate biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 1 (EC:2.8.2.21)
Alternative name(s):
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1
Short name:
GST-1
Keratan sulfate Gal-6 sulfotransferase
Short name:
KS6ST
Short name:
KSGal6ST
Short name:
KSST
Gene namesi
Name:Chst1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 3

Organism-specific databases

RGDi1308142. Chst1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 11CytoplasmicSequence Analysis
Transmembranei2 – 2322Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini24 – 411388LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 411411Carbohydrate sulfotransferase 1PRO_0000085184Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence Analysis
Glycosylationi189 – 1891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi334 – 3341N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ5RJQ0.

Expressioni

Gene expression databases

GenevestigatoriQ5RJQ0.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010510.

Structurei

3D structure databases

ProteinModelPortaliQ5RJQ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi337 – 3393Cell attachment siteSequence Analysis

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG80862.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG106811.
InParanoidiQ5RJQ0.
KOiK01022.
OMAiCEEGPTF.
OrthoDBiEOG7RZ5S0.
PhylomeDBiQ5RJQ0.
TreeFamiTF342871.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Q5RJQ0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQCSWKAVLL LALASIAIQY TAIRTFTAKS FHTCPGLTET GLAERLCEEG
60 70 80 90 100
PTFSYNLSRK THVLILATTR SGSSFVGQLF NQHMDVFYLF EPLYHVQNTL
110 120 130 140 150
IPRFTQGKSP ADRRVMLGAS RDLLRSLYDC DLYFLENYIK PPPVNHTTDR
160 170 180 190 200
VFRRGASRVL CSRPVCDPPG ASDLILEEGD CVRKCGLLNL TLAAEACRER
210 220 230 240 250
SHVAIKTVRV PEVNDLRALV EDPRLNLKVI QLVRDPRGIL ASRSETFRDT
260 270 280 290 300
YRLWRLWYGT GRKPYNLDVT QLTTVCEDFS SSVSTGLMRP SWLKGKYMLV
310 320 330 340 350
RYEDLARNPM KKTEEIYEFL GIPLDSHVAR WIQNNTRGDP TLGKHKYGTV
360 370 380 390 400
RNSAATAEKW RFRLSYDIVA FAQNACQHVL AQLGYKMATS EEELKNPAIS
410
LVEERDFRPF L
Length:411
Mass (Da):46,885
Last modified:December 21, 2004 - v1
Checksum:i715998736106E5CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC086551 mRNA. Translation: AAH86551.1.
RefSeqiNP_001011955.1. NM_001011955.1.
XP_006234611.1. XM_006234549.2.
XP_008760245.1. XM_008762023.1.
UniGeneiRn.16695.

Genome annotation databases

EnsembliENSRNOT00000010510; ENSRNOP00000010510; ENSRNOG00000007989.
GeneIDi295934.
KEGGirno:295934.
UCSCiRGD:1308142. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC086551 mRNA. Translation: AAH86551.1.
RefSeqiNP_001011955.1. NM_001011955.1.
XP_006234611.1. XM_006234549.2.
XP_008760245.1. XM_008762023.1.
UniGeneiRn.16695.

3D structure databases

ProteinModelPortaliQ5RJQ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010510.

Proteomic databases

PaxDbiQ5RJQ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010510; ENSRNOP00000010510; ENSRNOG00000007989.
GeneIDi295934.
KEGGirno:295934.
UCSCiRGD:1308142. rat.

Organism-specific databases

CTDi8534.
RGDi1308142. Chst1.

Phylogenomic databases

eggNOGiNOG80862.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG106811.
InParanoidiQ5RJQ0.
KOiK01022.
OMAiCEEGPTF.
OrthoDBiEOG7RZ5S0.
PhylomeDBiQ5RJQ0.
TreeFamiTF342871.

Enzyme and pathway databases

ReactomeiREACT_196475. Keratan sulfate biosynthesis.

Miscellaneous databases

NextBioi640351.
PROiQ5RJQ0.

Gene expression databases

GenevestigatoriQ5RJQ0.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiCHST1_RAT
AccessioniPrimary (citable) accession number: Q5RJQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: December 21, 2004
Last modified: January 7, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.