Q5RJM0 (MK67I_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MKI67 FHA domain-interacting nucleolar phosphoprotein

Alternative name(s):
Nucleolar protein interacting with the FHA domain of pKI-67

Gene names

Name:	Mki67ip
Synonyms:	Nifk

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subunit structure	Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis By similarity.
Subcellular location	Nucleus › nucleolus. Chromosome By similarity. Note: Localizes to mitotic chromosomes in conjunction with MKI67 By similarity.
Post-translational modification	Phosphorylated By similarity.
Sequence similarities	Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Cellular component	Chromosome Nucleus
Ligand	RNA-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	condensed nuclear chromosome Inferred from electronic annotation. Source: Compara cytoplasm Inferred from electronic annotation. Source: Compara nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell nucleoplasm Inferred from electronic annotation. Source: Compara
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 271	270	MKI67 FHA domain-interacting nucleolar phosphoprotein		PRO_0000247991
Regions
	Domain	44 – 122	79	RRM
	Compositional bias	34 – 37	4	Poly-Lys
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	213	1	Phosphothreonine By similarity
	Modified residue	217	1	Phosphothreonine By similarity
	Modified residue	226	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5RJM0 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 14472E53C24EC24E	FASTA	271	31,351
	10 20 30 40 50 60 MAEYSGPAKP TLALNPREDS QFEKDLTQIQ RRAKKKKEEK LNSGVVYLGH LPSTLSESHI 70 80 90 100 110 120 YDYCAQFGDI RRFRLSRSKR TGNSRGFAFV EFESEDVAKI VAETMDNYLF GERLLSCKFM 130 140 150 160 170 180 PREKVHKDLF NQCNVPFHPP SFPAVKRYNQ KRGHLQMLKM EYRFKKKEKL LRKKLAKKGI 190 200 210 220 230 240 DYSFPSLVLP KPKKEISSIA NTHGDSEANQ DPTPVCTPTF LERRKSQLME INDDDEIILK 250 260 270 LPVSPVKEDT QKTPAPESSG KKRLRKRKSK Q

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC086585 mRNA. Translation: AAH86585.1.
IPI	IPI00389917.
RefSeq	NP_631925.2. NM_139186.2.
UniGene	Rn.20901.
3D structure databases
HSSP	HSSP built from PDB template 2AFF based on UniProtKB Q9BYG3.
ProteinModelPortal	Q5RJM0.
SMR	Q5RJM0. Positions 209-243.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000058336.
Proteomic databases
PaxDb	Q5RJM0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000061619; ENSRNOP00000058336; ENSRNOG00000025701.
GeneID	246042.
KEGG	rno:246042.
Organism-specific databases
CTD	84365.
RGD	708462. Mki67ip.
Phylogenomic databases
eggNOG	NOG244773.
GeneTree	ENSGT00390000011515.
HOGENOM	HOG000242795.
HOVERGEN	HBG057796.
KO	K14838.
OrthoDB	EOG4MKNGX.
Gene expression databases
Genevestigator	Q5RJM0.
GermOnline	ENSRNOG00000025701. Rattus norvegicus.
Family and domain databases
Gene3D	3.30.70.330. 1 hit.
InterPro	IPR021043. hNIFK_FHA_Ki67_binding. IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Pfam	PF12196. hNIFK_binding. 1 hit. PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	623295.

Entry information

Entry name

MK67I_RAT

Accession

Primary (citable) accession number: Q5RJM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	December 21, 2004
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program