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Q5RJI4

- PKDCC_MOUSE

UniProt

Q5RJI4 - PKDCC_MOUSE

Protein

Protein kinase domain-containing protein, cytoplasmic

Gene

Pkdcc

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 2 (23 Mar 2010)
      Previous versions | rss
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    Functioni

    Protein kinase which is required for longitudinal bone growth through regulation of chondrocyte differentiation. Involved in protein transport from the Golgi apparatus to the plasma membrane.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei166 – 1661ATPPROSITE-ProRule annotation
    Active sitei278 – 2781Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi144 – 1529ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein kinase activity Source: UniProtKB

    GO - Biological processi

    1. bone mineralization Source: MGI
    2. cell differentiation Source: UniProtKB-KW
    3. embryonic digestive tract development Source: UniProtKB
    4. lung alveolus development Source: UniProtKB
    5. multicellular organism growth Source: MGI
    6. negative regulation of Golgi to plasma membrane protein transport Source: UniProtKB
    7. palate development Source: UniProtKB
    8. positive regulation of bone mineralization Source: UniProtKB
    9. positive regulation of chondrocyte differentiation Source: UniProtKB
    10. protein transport Source: UniProtKB-KW
    11. skeletal system development Source: MGI

    Keywords - Molecular functioni

    Developmental protein, Kinase, Transferase

    Keywords - Biological processi

    Differentiation, Osteogenesis, Protein transport, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein kinase domain-containing protein, cytoplasmic (EC:2.7.-.-)
    Alternative name(s):
    Protein kinase-like protein SgK493
    Sugen kinase 493
    Vertebrate lonesome kinase
    Gene namesi
    Name:Pkdcc
    Synonyms:Sgk493, Vlk
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:2147077. Pkdcc.

    Subcellular locationi

    Golgi apparatus 1 Publication

    GO - Cellular componenti

    1. Golgi apparatus Source: UniProtKB

    Keywords - Cellular componenti

    Golgi apparatus

    Pathology & Biotechi

    Disruption phenotypei

    Morphological defects at birth including growth retardation, short limbs, cleft palate, sternal dysraphia, shortened intestine and cyanosis. Long bones display reduced mineralization due to delayed chondrocyte differentiation. Neonates breathe abnormally, do not suckle and die within a day after birth, probably due to insufficient respiration as a result of the cleft palate.2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi166 – 1661K → M: Complete loss of phosphorylation. Phosphorylation is restored by co-transfection with wild-type protein. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 492492Protein kinase domain-containing protein, cytoplasmicPRO_0000263011Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei148 – 1481Phosphotyrosine1 Publication
    Modified residuei177 – 1771Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ5RJI4.

    PTM databases

    PhosphoSiteiQ5RJI4.

    Expressioni

    Tissue specificityi

    Strongly expressed in adult heart, liver and testis with weak expression in brain, spleen, lung and thymus. In the humerus, strongly expressed in early flat proliferative chondrocytes. In the embryo, expressed in the anterior visceral endoderm and anterior primitive streak at E6.5. At E7.5, expressed in the anterior definitive endoderm (ADE) and anterior mesoderm but not in the notochord. At E8.0, expressed in the ADE and anterior embryonic mesoderm. At E8.5, expressed more broadly in anterior tissues and at the midline of the neural plate in the midbrain region as well as the lateral margins of the neural plate posterior to the metencephalic region. Also weakly expressed in the anterior mesenchyme. At E9.5, strongest expression in branchial arches and limb buds. During mid-gestation, expression continues in mesenchymal cells, particularly in areas where these cells condense.2 Publications

    Developmental stagei

    Expressed throughout embryogenesis and in adulthood. During embryogenesis, expression is high at transition phases of mesenchymal cell differentiation such as from mesenchymal cells to chondrocytes and from mesenchymal to mesothelial cells. Expressed throughout all stages of humerus bone formation. During lung development, expressed in all mesenchymal cells at the early pseudoglandular stage. Expression is rapidly lost from the mesenchyme of the lung interstitium during the mid-to-late pseudoglandular stage but continues throughout life in the mesothelial pleural cells.2 Publications

    Gene expression databases

    BgeeiQ5RJI4.
    GenevestigatoriQ5RJI4.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000025094.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5RJI4.
    SMRiQ5RJI4. Positions 133-345.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini138 – 422285Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi75 – 12854Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily.Curated
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG254379.
    GeneTreeiENSGT00390000001205.
    HOGENOMiHOG000054185.
    HOVERGENiHBG079871.
    InParanoidiQ5RJI4.
    KOiK17548.
    OMAiYVGSGYT.
    OrthoDBiEOG7034GV.
    PhylomeDBiQ5RJI4.
    TreeFamiTF329248.

    Family and domain databases

    InterProiIPR022049. FAM69_kinase_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    [Graphical view]
    PfamiPF12260. PIP49_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q5RJI4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRRRRAAVAA GFCASFLLGS VLNVLFAPGS EPPRPGQSPG SSAAPGPGRR    50
    GGRGELARQI RERYEEVQRY SRGGPGPGAG RPERRRLMDL APGGPGLQRP 100
    RPPRVRSPPD GAPGWPPAPG PGSPGPGPRL GCAALRNVSG AQYVGSGYTK 150
    AVYRVRLPGG AAVALKAVDF SGHDLGSCVR EFGARRGCYR LAAHKLLKEM 200
    VLLERLRHPN VLQLYGYCYQ DSEGIPDTLT TITELGAPVE MIQLLQTSWE 250
    DRFRICLSLG RLLHHLAHSP LGSVTLLDFR PRQFVLVNGE LKVTDLDDAR 300
    VEETPCTSSA DCTLEFPARN FSLPCSAQGW CEGMNEKRNL YNAYRFFFTY 350
    LLPHSAPPSL RPLLDSIVNA TGELAWGVDE TLAQLETALH LFRSGQYLQN 400
    STSSRAEYQR IPDSAITQED YRCWPSYHHG GCLLSVFNLA EAIDVCESHA 450
    QCRAFVVTNQ TTWTGRKLVF FKTGWNQVVP DAGKTTYVKA PG 492
    Length:492
    Mass (Da):53,841
    Last modified:March 23, 2010 - v2
    Checksum:iAD95CAF730625FF9
    GO
    Isoform 2 (identifier: Q5RJI4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         72-118: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:445
    Mass (Da):48,988
    Checksum:i5329234F964F15E9
    GO

    Sequence cautioni

    The sequence AAH86639.1 differs from that shown. Reason: Frameshift at position 72.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei72 – 11847Missing in isoform 2. 1 PublicationVSP_038822Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB436780 mRNA. Translation: BAG82823.1.
    AB381893 mRNA. Translation: BAH66379.1.
    BC022157 mRNA. Translation: AAH22157.1.
    BC086639 mRNA. Translation: AAH86639.1. Frameshift.
    AK133913 mRNA. Translation: BAE21924.1.
    CCDSiCCDS28994.2. [Q5RJI4-1]
    RefSeqiNP_598878.2. NM_134117.2. [Q5RJI4-1]
    UniGeneiMm.311974.

    Genome annotation databases

    EnsembliENSMUST00000170794; ENSMUSP00000129238; ENSMUSG00000024247. [Q5RJI4-1]
    GeneIDi106522.
    KEGGimmu:106522.
    UCSCiuc012axu.1. mouse. [Q5RJI4-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB436780 mRNA. Translation: BAG82823.1 .
    AB381893 mRNA. Translation: BAH66379.1 .
    BC022157 mRNA. Translation: AAH22157.1 .
    BC086639 mRNA. Translation: AAH86639.1 . Frameshift.
    AK133913 mRNA. Translation: BAE21924.1 .
    CCDSi CCDS28994.2. [Q5RJI4-1 ]
    RefSeqi NP_598878.2. NM_134117.2. [Q5RJI4-1 ]
    UniGenei Mm.311974.

    3D structure databases

    ProteinModelPortali Q5RJI4.
    SMRi Q5RJI4. Positions 133-345.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000025094.

    PTM databases

    PhosphoSitei Q5RJI4.

    Proteomic databases

    PRIDEi Q5RJI4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000170794 ; ENSMUSP00000129238 ; ENSMUSG00000024247 . [Q5RJI4-1 ]
    GeneIDi 106522.
    KEGGi mmu:106522.
    UCSCi uc012axu.1. mouse. [Q5RJI4-1 ]

    Organism-specific databases

    CTDi 91461.
    MGIi MGI:2147077. Pkdcc.

    Phylogenomic databases

    eggNOGi NOG254379.
    GeneTreei ENSGT00390000001205.
    HOGENOMi HOG000054185.
    HOVERGENi HBG079871.
    InParanoidi Q5RJI4.
    KOi K17548.
    OMAi YVGSGYT.
    OrthoDBi EOG7034GV.
    PhylomeDBi Q5RJI4.
    TreeFami TF329248.

    Miscellaneous databases

    NextBioi 358240.
    PROi Q5RJI4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q5RJI4.
    Genevestigatori Q5RJI4.

    Family and domain databases

    InterProi IPR022049. FAM69_kinase_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    [Graphical view ]
    Pfami PF12260. PIP49_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Short limbs, cleft palate, and delayed formation of flat proliferative chondrocytes in mice with targeted disruption of a putative protein kinase gene, Pkdcc (AW548124)."
      Imuta Y., Nishioka N., Kiyonari H., Sasaki H.
      Dev. Dyn. 238:210-222(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, ALTERNATIVE SPLICING.
      Tissue: Embryo.
    2. "The novel protein kinase Vlk is essential for stromal function of mesenchymal cells."
      Kinoshita M., Era T., Jakt L.M., Nishikawa S.
      Development 136:2069-2079(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, PHOSPHORYLATION AT TYR-148 AND SER-177, MUTAGENESIS OF LYS-166.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6 and FVB/N.
      Tissue: Brain and Liver.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 381-492 (ISOFORMS 1/2).
      Strain: C57BL/6J.
      Tissue: Embryo.

    Entry informationi

    Entry nameiPKDCC_MOUSE
    AccessioniPrimary (citable) accession number: Q5RJI4
    Secondary accession number(s): B6F136, Q3UZD3, Q8VBZ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: March 23, 2010
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3