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Protein

Protein SMG7

Gene

Smg7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Nonsense-mediated mRNA decay

Enzyme and pathway databases

ReactomeiR-MMU-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SMG7
Alternative name(s):
EST1-like protein C
SMG-7 homolog
Gene namesi
Name:Smg7
Synonyms:Est1c, Kiaa0250
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2682334. Smg7.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Predominantly cytoplasmic, and nuclear. Shuttles between nucleus and cytoplasm (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 11381137Protein SMG7PRO_0000076325Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei519 – 5191PhosphoserineBy similarity
Modified residuei575 – 5751PhosphothreonineBy similarity
Modified residuei732 – 7321PhosphoserineBy similarity
Modified residuei848 – 8481PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ5RJH6.
MaxQBiQ5RJH6.
PaxDbiQ5RJH6.
PeptideAtlasiQ5RJH6.
PRIDEiQ5RJH6.

PTM databases

iPTMnetiQ5RJH6.
PhosphoSiteiQ5RJH6.

Miscellaneous databases

PMAP-CutDBQ5RJH6.

Expressioni

Gene expression databases

BgeeiQ5RJH6.
GenevisibleiQ5RJH6. MM.

Interactioni

Subunit structurei

Part of a complex that contains SMG5, SMG7, PPP2CA, a short isoform of UPF3A (isoform UPF3AS, but not isoform UPF3AL) and phosphorylated UPF1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041241.

Structurei

3D structure databases

ProteinModelPortaliQ5RJH6.
SMRiQ5RJH6. Positions 1-496.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati152 – 18534TPR 1Add
BLAST
Repeati187 – 21933TPR 2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi599 – 794196Gln/Pro-richAdd
BLAST
Compositional biasi923 – 101694Ser-richAdd
BLAST

Sequence similaritiesi

Contains 2 TPR repeats.Curated

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiENOG410INQX. Eukaryota.
ENOG410XQ61. LUCA.
GeneTreeiENSGT00560000077120.
HOGENOMiHOG000049052.
HOVERGENiHBG056330.
InParanoidiQ5RJH6.
KOiK14409.
OMAiQCENEVG.
OrthoDBiEOG708VZ1.
PhylomeDBiQ5RJH6.
TreeFamiTF327119.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR018834. DNA/RNA-bd_Est1-type.
IPR019458. EST1.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF10374. EST1. 1 hit.
PF10373. EST1_DNA_bind. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5RJH6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLQSAQYLR QAEVLKAEMT DSKLGPAEVW TSRQALQDLY QKMLVTDLEY
60 70 80 90 100
ALDKKVEQDL WNHAFKNQIT TLQGQAKNRA NPNRSEVQAN LSLFLEAASG
110 120 130 140 150
FYTQLLQELC TVFNVDLPCR VKSSQLGIIS NKQTHSSTIV KPQSSSCSYI
160 170 180 190 200
CQHCLVHLGD IARYRNQTSQ AESYYRHAAQ LVPSNGQPYN QLAILASSKG
210 220 230 240 250
DHLTTIFYYC RSIAVKFPFP AASTNLQKAL SKALESRDEL KTKWGVSDFI
260 270 280 290 300
KAFIKFHGHV YLSKSLEKLS PLREKLEEQF KRLLFQKAFN SQQLVHVTVI
310 320 330 340 350
NLFQLHHLRD FSNETEQHSY SQDEQLCWTQ LLALFMSFLG ILCKCPLQND
360 370 380 390 400
SQESNNAYPL PAVKVSMDWL RLRPRVFQEA VVDERQYIWP WLISLLNSFH
410 420 430 440 450
PREDDLSNTN ATPLPEEFEL QGFLALRPSF RNLDFSKGHQ GITGDKEGQQ
460 470 480 490 500
RRIRQQRLIS IGKWIADNQP RLIQCENEVG KLLFITEIPE LILEDPSEAK
510 520 530 540 550
ENLILQETSV VESLATDGSP GLKSVLSTGR NPSNSCDSGE KPVVTFKENI
560 570 580 590 600
KPREVNQGRS FPPKEVKSQT ELRKTPVSEA RKTPVTQTPS QTSNSQFIPI
610 620 630 640 650
HHPGAFPPLP SRPGFPPPTY VIPPPVAFSM GSGYTFPAGV SVPGTFLQST
660 670 680 690 700
AHSPAGNQVQ AGKQSHIPYS QQRPSGPGPM NQGPQQSQPP SQPPLTSLPA
710 720 730 740 750
QPTAQSTSQL QVQALAQQQQ SPTKVIPALG KSPPHHSGFQ QYQQADASKQ
760 770 780 790 800
LWNPPQVQSP LGKIMPVKQS YYLQTQDPIK LFEPSLQPPV IQQQPLEKKM
810 820 830 840 850
KPFPMEPYNH NPSEVKVPEF YWDSSYSMAD NRAVMAQQPN MDRRSKRSPG
860 870 880 890 900
VFRPEQDPVP RMPFEDPKSS PLLPPDLLKS LAALEEEEEL IFSNPPDLYP
910 920 930 940 950
ALLGPLASLP GRSLFKSLLE KPSELMSHSS SFLSLTGFSV NQERYPNSSM
960 970 980 990 1000
FNEVYGKNLT TSSKAELNPS VASQETSLYS LFEGTPWSPS LPASSDHSTP
1010 1020 1030 1040 1050
ASQSPHSSNP SSLPSSPPTH NHNSAPFSNF GPIGTPDNRD RRPADRWKTD
1060 1070 1080 1090 1100
KPAMGGFGVD YLSATSSSES SWHQASTPSG TWTGHGPSME DSSAVLMESL
1110 1120 1130
KSIWSSSMMH PGPSALEQLL MQQKQKQQRG QGAMNPPH
Length:1,138
Mass (Da):126,841
Last modified:December 21, 2004 - v1
Checksum:i330576E241E35AD4
GO
Isoform 2 (identifier: Q5RJH6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: MSLQSAQYL → MRTENLKSEEHLKSSNI

Show »
Length:1,146
Mass (Da):127,817
Checksum:i462775EB9EC356F4
GO
Isoform 3 (identifier: Q5RJH6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     566-566: V → VRRDCSKGVTVTQEDGQKDSSKRRAETKRCTLGKLQETGKQSVAVQV
     866-915: Missing.

Show »
Length:1,134
Mass (Da):126,563
Checksum:i7F2A9BC1BE152A66
GO

Sequence cautioni

The sequence BAC97911.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti552 – 5521P → S in BAC97911 (PubMed:14621295).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 99MSLQSAQYL → MRTENLKSEEHLKSSNI in isoform 2. 1 PublicationVSP_016577
Alternative sequencei566 – 5661V → VRRDCSKGVTVTQEDGQKDS SKRRAETKRCTLGKLQETGK QSVAVQV in isoform 3. 1 PublicationVSP_016578
Alternative sequencei866 – 91550Missing in isoform 3. 1 PublicationVSP_016579Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129101 mRNA. Translation: BAC97911.1. Different initiation.
BC082789 mRNA. Translation: AAH82789.1.
BC086651 mRNA. Translation: AAH86651.1.
CCDSiCCDS35740.1. [Q5RJH6-3]
CCDS48394.1. [Q5RJH6-2]
CCDS48395.1. [Q5RJH6-1]
RefSeqiNP_001005507.1. NM_001005507.2. [Q5RJH6-3]
NP_001153728.1. NM_001160256.1. [Q5RJH6-1]
NP_001153729.1. NM_001160257.1. [Q5RJH6-2]
XP_006529515.1. XM_006529452.2. [Q5RJH6-2]
UniGeneiMm.270775.

Genome annotation databases

EnsembliENSMUST00000043560; ENSMUSP00000041241; ENSMUSG00000042772. [Q5RJH6-2]
ENSMUST00000073441; ENSMUSP00000073144; ENSMUSG00000042772. [Q5RJH6-3]
ENSMUST00000111836; ENSMUSP00000107467; ENSMUSG00000042772. [Q5RJH6-1]
GeneIDi226517.
KEGGimmu:226517.
UCSCiuc007czn.1. mouse. [Q5RJH6-2]
uc007czo.2. mouse. [Q5RJH6-3]
uc007czp.2. mouse. [Q5RJH6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129101 mRNA. Translation: BAC97911.1. Different initiation.
BC082789 mRNA. Translation: AAH82789.1.
BC086651 mRNA. Translation: AAH86651.1.
CCDSiCCDS35740.1. [Q5RJH6-3]
CCDS48394.1. [Q5RJH6-2]
CCDS48395.1. [Q5RJH6-1]
RefSeqiNP_001005507.1. NM_001005507.2. [Q5RJH6-3]
NP_001153728.1. NM_001160256.1. [Q5RJH6-1]
NP_001153729.1. NM_001160257.1. [Q5RJH6-2]
XP_006529515.1. XM_006529452.2. [Q5RJH6-2]
UniGeneiMm.270775.

3D structure databases

ProteinModelPortaliQ5RJH6.
SMRiQ5RJH6. Positions 1-496.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041241.

PTM databases

iPTMnetiQ5RJH6.
PhosphoSiteiQ5RJH6.

Proteomic databases

EPDiQ5RJH6.
MaxQBiQ5RJH6.
PaxDbiQ5RJH6.
PeptideAtlasiQ5RJH6.
PRIDEiQ5RJH6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043560; ENSMUSP00000041241; ENSMUSG00000042772. [Q5RJH6-2]
ENSMUST00000073441; ENSMUSP00000073144; ENSMUSG00000042772. [Q5RJH6-3]
ENSMUST00000111836; ENSMUSP00000107467; ENSMUSG00000042772. [Q5RJH6-1]
GeneIDi226517.
KEGGimmu:226517.
UCSCiuc007czn.1. mouse. [Q5RJH6-2]
uc007czo.2. mouse. [Q5RJH6-3]
uc007czp.2. mouse. [Q5RJH6-1]

Organism-specific databases

CTDi9887.
MGIiMGI:2682334. Smg7.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410INQX. Eukaryota.
ENOG410XQ61. LUCA.
GeneTreeiENSGT00560000077120.
HOGENOMiHOG000049052.
HOVERGENiHBG056330.
InParanoidiQ5RJH6.
KOiK14409.
OMAiQCENEVG.
OrthoDBiEOG708VZ1.
PhylomeDBiQ5RJH6.
TreeFamiTF327119.

Enzyme and pathway databases

ReactomeiR-MMU-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PMAP-CutDBQ5RJH6.
PROiQ5RJH6.
SOURCEiSearch...

Gene expression databases

BgeeiQ5RJH6.
GenevisibleiQ5RJH6. MM.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR018834. DNA/RNA-bd_Est1-type.
IPR019458. EST1.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF10374. EST1. 1 hit.
PF10373. EST1_DNA_bind. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: C57BL/6J.
    Tissue: Brain and Embryonic germ cell.

Entry informationi

Entry nameiSMG7_MOUSE
AccessioniPrimary (citable) accession number: Q5RJH6
Secondary accession number(s): Q63ZW5, Q6ZQF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 21, 2004
Last modified: July 6, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.