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Protein

Isoprenoid synthase domain-containing protein

Gene

Ispd

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for protein O-linked mannosylation. Probably acts as a nucleotidyltransferase involved in synthesis of a nucleotide sugar. Required for dystroglycan O-mannosylation (By similarity).By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei57 – 571Transition state stabilizerBy similarity
Sitei64 – 641Transition state stabilizerBy similarity
Sitei203 – 2031Positions substrate for the nucleophilic attackBy similarity
Sitei261 – 2611Positions substrate for the nucleophilic attackBy similarity

GO - Molecular functioni

GO - Biological processi

  • axon guidance Source: MGI
  • isoprenoid biosynthetic process Source: InterPro
  • protein glycosylation Source: MGI
  • protein O-linked mannosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoprenoid synthase domain-containing protein (EC:2.7.7.-)
Alternative name(s):
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like protein
Gene namesi
Name:Ispd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1923097. Ispd.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447Isoprenoid synthase domain-containing proteinPRO_0000343698Add
BLAST

Proteomic databases

MaxQBiQ5RJG7.
PaxDbiQ5RJG7.
PRIDEiQ5RJG7.

PTM databases

PhosphoSiteiQ5RJG7.

Expressioni

Gene expression databases

BgeeiQ5RJG7.
GenevisibleiQ5RJG7. MM.

Interactioni

Protein-protein interaction databases

IntActiQ5RJG7. 1 interaction.
MINTiMINT-4116353.
STRINGi10090.ENSMUSP00000061646.

Structurei

3D structure databases

ProteinModelPortaliQ5RJG7.
SMRiQ5RJG7. Positions 42-447.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IspD family.Curated

Phylogenomic databases

eggNOGiENOG410IIH2. Eukaryota.
COG1211. LUCA.
GeneTreeiENSGT00390000006412.
HOVERGENiHBG057782.
InParanoidiQ5RJG7.
OMAiCYNFVCV.
OrthoDBiEOG7KDFB2.
PhylomeDBiQ5RJG7.
TreeFamiTF328415.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001228. IspD.
IPR018294. ISPD_synthase_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS01295. ISPD. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5RJG7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPGPCSRPA EPGHCVSGPA GAGSAFPESP LSVAGAEPGN RPGTVAAVLP
60 70 80 90 100
AGGCGERMGV RTPKQFCRVL ERPLISYTLQ AMERVCWIKD IVVTVTGENM
110 120 130 140 150
EAMRSIIQRY GHKRISLAEA GATRHRSIFN GLKALAEDQP DCKLTKPEVV
160 170 180 190 200
IIHDAVRPFV EEDILLRVVL AAKEHGAAGA IRPLVSTVIS PSADGHLDHS
210 220 230 240 250
LDRAKHRASE MPQAFLFDVI YEAYQQCSDF DLEFGTECLQ LALKYCHRKA
260 270 280 290 300
KLVEGPPALW KVTYKQDLCA AEAMIKEKIS QEICVVMNTK DEESVGHLLE
310 320 330 340 350
EALRKELNCM KITSTVMDHI GGDIRNFIEQ CYSFICVNVV SPDSQETRKL
360 370 380 390 400
LRILEESSLP LLYPVVVVLV HCFDFTSVPL AQKMESLVWI RGLAKEVKER
410 420 430 440
NILLSGLLLN YSQDEQKLQE SLGQSAAIIA ALVKERNSAL VGQLLVA
Length:447
Mass (Da):49,136
Last modified:December 21, 2004 - v1
Checksum:iAD883B5816D222AB
GO
Isoform 2 (identifier: Q5RJG7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     309-324: CMKITSTVMDHIGGDI → VSIQRMTWIKCHTFLW
     325-447: Missing.

Show »
Length:324
Mass (Da):35,695
Checksum:i4085DE834F5F32C3
GO
Isoform 3 (identifier: Q5RJG7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     277-286: EKISQEICVV → GVFNLVTVSA
     287-447: Missing.

Show »
Length:286
Mass (Da):30,985
Checksum:iFE87DDD1D5CFE53B
GO
Isoform 4 (identifier: Q5RJG7-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-99: Missing.
     277-286: EKISQEICVV → GVFNLVTVSA
     287-447: Missing.

Show »
Length:187
Mass (Da):20,770
Checksum:iCEDC60A2D423E288
GO
Isoform 5 (identifier: Q5RJG7-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     414-419: DEQKLQ → NFYYGG
     420-447: Missing.

Show »
Length:419
Mass (Da):46,292
Checksum:iFF280F53E42D031F
GO

Sequence cautioni

The sequence BAC32538.1 differs from that shown. Reason: Frameshift at position 48. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti178 – 1781A → E in BAC31463 (PubMed:16141072).Curated
Sequence conflicti228 – 2281S → N in AAI18039 (PubMed:15489334).Curated
Sequence conflicti342 – 3421P → L in AAH24061 (PubMed:15489334).Curated
Sequence conflicti352 – 3521R → H in AAH24061 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9999Missing in isoform 4. 1 PublicationVSP_034667Add
BLAST
Alternative sequencei277 – 28610EKISQEICVV → GVFNLVTVSA in isoform 3 and isoform 4. 2 PublicationsVSP_034668
Alternative sequencei287 – 447161Missing in isoform 3 and isoform 4. 2 PublicationsVSP_034669Add
BLAST
Alternative sequencei309 – 32416CMKIT…IGGDI → VSIQRMTWIKCHTFLW in isoform 2. 2 PublicationsVSP_034670Add
BLAST
Alternative sequencei325 – 447123Missing in isoform 2. 2 PublicationsVSP_034671Add
BLAST
Alternative sequencei414 – 4196DEQKLQ → NFYYGG in isoform 5. 1 PublicationVSP_034672
Alternative sequencei420 – 44728Missing in isoform 5. 1 PublicationVSP_034673Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK039004 mRNA. Translation: BAC30199.1.
AK043122 mRNA. Translation: BAC31463.1.
AK045940 mRNA. Translation: BAC32538.1. Frameshift.
BC024061 mRNA. Translation: AAH24061.1.
BC086665 mRNA. Translation: AAH86665.1.
BC096030 mRNA. Translation: AAH96030.1.
BC118038 mRNA. Translation: AAI18039.1.
CCDSiCCDS49051.1. [Q5RJG7-1]
RefSeqiNP_001276431.1. NM_001289502.1.
NP_001276432.1. NM_001289503.1.
NP_001276433.1. NM_001289504.1. [Q5RJG7-3]
NP_848744.2. NM_178629.6. [Q5RJG7-1]
XP_006515307.1. XM_006515244.2. [Q5RJG7-5]
UniGeneiMm.261764.

Genome annotation databases

EnsembliENSMUST00000062041; ENSMUSP00000061646; ENSMUSG00000043153. [Q5RJG7-1]
GeneIDi75847.
KEGGimmu:75847.
UCSCiuc007njy.3. mouse. [Q5RJG7-3]
uc007njz.3. mouse. [Q5RJG7-2]
uc007nkb.3. mouse. [Q5RJG7-1]
uc007nkd.1. mouse. [Q5RJG7-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK039004 mRNA. Translation: BAC30199.1.
AK043122 mRNA. Translation: BAC31463.1.
AK045940 mRNA. Translation: BAC32538.1. Frameshift.
BC024061 mRNA. Translation: AAH24061.1.
BC086665 mRNA. Translation: AAH86665.1.
BC096030 mRNA. Translation: AAH96030.1.
BC118038 mRNA. Translation: AAI18039.1.
CCDSiCCDS49051.1. [Q5RJG7-1]
RefSeqiNP_001276431.1. NM_001289502.1.
NP_001276432.1. NM_001289503.1.
NP_001276433.1. NM_001289504.1. [Q5RJG7-3]
NP_848744.2. NM_178629.6. [Q5RJG7-1]
XP_006515307.1. XM_006515244.2. [Q5RJG7-5]
UniGeneiMm.261764.

3D structure databases

ProteinModelPortaliQ5RJG7.
SMRiQ5RJG7. Positions 42-447.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ5RJG7. 1 interaction.
MINTiMINT-4116353.
STRINGi10090.ENSMUSP00000061646.

PTM databases

PhosphoSiteiQ5RJG7.

Proteomic databases

MaxQBiQ5RJG7.
PaxDbiQ5RJG7.
PRIDEiQ5RJG7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000062041; ENSMUSP00000061646; ENSMUSG00000043153. [Q5RJG7-1]
GeneIDi75847.
KEGGimmu:75847.
UCSCiuc007njy.3. mouse. [Q5RJG7-3]
uc007njz.3. mouse. [Q5RJG7-2]
uc007nkb.3. mouse. [Q5RJG7-1]
uc007nkd.1. mouse. [Q5RJG7-4]

Organism-specific databases

CTDi729920.
MGIiMGI:1923097. Ispd.

Phylogenomic databases

eggNOGiENOG410IIH2. Eukaryota.
COG1211. LUCA.
GeneTreeiENSGT00390000006412.
HOVERGENiHBG057782.
InParanoidiQ5RJG7.
OMAiCYNFVCV.
OrthoDBiEOG7KDFB2.
PhylomeDBiQ5RJG7.
TreeFamiTF328415.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

PROiQ5RJG7.
SOURCEiSearch...

Gene expression databases

BgeeiQ5RJG7.
GenevisibleiQ5RJG7. MM.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001228. IspD.
IPR018294. ISPD_synthase_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS01295. ISPD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 4 AND 5).
    Strain: C57BL/6J.
    Tissue: Cerebellum, Corpora quadrigemina and Hypothalamus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: C57BL/6J and Czech II.
    Tissue: Eye and Mammary tumor.

Entry informationi

Entry nameiISPD_MOUSE
AccessioniPrimary (citable) accession number: Q5RJG7
Secondary accession number(s): Q148Q0
, Q501J8, Q8BR14, Q8C934, Q8CAE0, Q8CIF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: December 21, 2004
Last modified: June 8, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.