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Protein

D-ribitol-5-phosphate cytidylyltransferase

Gene

Ispd

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Cytidylyltransferase required for protein O-linked mannosylation. Catalyzes the formation of CDP-ribitol nucleotide sugar from D-ribitol 5-phosphate. CDP-ribitol is a substrate of FKTN during the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Shows activity toward other pentose phosphate sugars and mediates formation of CDP-ribulose or CDP-ribose using CTP and ribulose-5-phosphate or ribose-5-phosphate, respectively. Not Involved in dolichol production.By similarity

Catalytic activityi

CTP + D-ribitol 5-phosphate = diphosphate + CDP-ribitol.By similarity
CTP + D-ribose 5-phosphate = diphosphate + CDP-ribose.By similarity
CTP + D-ribulose 5-phosphate = diphosphate + CDP-ribulose.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei57Transition state stabilizerBy similarity1
Sitei64Transition state stabilizerBy similarity1
Sitei203Positions substrate for the nucleophilic attackBy similarity1
Sitei261Positions substrate for the nucleophilic attackBy similarity1

GO - Molecular functioni

GO - Biological processi

  • axon guidance Source: MGI
  • isoprenoid biosynthetic process Source: InterPro
  • protein glycosylation Source: MGI
  • protein O-linked mannosylation Source: UniProtKB

Keywordsi

Molecular functionNucleotidyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
D-ribitol-5-phosphate cytidylyltransferaseBy similarity (EC:2.7.7.40By similarity)
Alternative name(s):
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like proteinBy similarity
Isoprenoid synthase domain-containing proteinImported
Gene namesi
Name:IspdImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1923097 Ispd

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003436981 – 447D-ribitol-5-phosphate cytidylyltransferaseAdd BLAST447

Proteomic databases

PaxDbiQ5RJG7
PRIDEiQ5RJG7

PTM databases

PhosphoSitePlusiQ5RJG7

Expressioni

Gene expression databases

BgeeiENSMUSG00000043153
ExpressionAtlasiQ5RJG7 baseline and differential
GenevisibleiQ5RJG7 MM

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ5RJG7, 1 interactor
MINTiQ5RJG7
STRINGi10090.ENSMUSP00000061646

Structurei

3D structure databases

ProteinModelPortaliQ5RJG7
SMRiQ5RJG7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IIH2 Eukaryota
COG1211 LUCA
GeneTreeiENSGT00390000006412
HOVERGENiHBG057782
InParanoidiQ5RJG7
KOiK21031
OMAiCYNFVCV
OrthoDBiEOG091G0JSI
PhylomeDBiQ5RJG7
TreeFamiTF328415

Family and domain databases

CDDicd02516 CDP-ME_synthetase, 1 hit
Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR034683 IspD/TarI
IPR018294 ISPD_synthase_CS
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF01128 IspD, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS01295 ISPD, 1 hit

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5RJG7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPGPCSRPA EPGHCVSGPA GAGSAFPESP LSVAGAEPGN RPGTVAAVLP
60 70 80 90 100
AGGCGERMGV RTPKQFCRVL ERPLISYTLQ AMERVCWIKD IVVTVTGENM
110 120 130 140 150
EAMRSIIQRY GHKRISLAEA GATRHRSIFN GLKALAEDQP DCKLTKPEVV
160 170 180 190 200
IIHDAVRPFV EEDILLRVVL AAKEHGAAGA IRPLVSTVIS PSADGHLDHS
210 220 230 240 250
LDRAKHRASE MPQAFLFDVI YEAYQQCSDF DLEFGTECLQ LALKYCHRKA
260 270 280 290 300
KLVEGPPALW KVTYKQDLCA AEAMIKEKIS QEICVVMNTK DEESVGHLLE
310 320 330 340 350
EALRKELNCM KITSTVMDHI GGDIRNFIEQ CYSFICVNVV SPDSQETRKL
360 370 380 390 400
LRILEESSLP LLYPVVVVLV HCFDFTSVPL AQKMESLVWI RGLAKEVKER
410 420 430 440
NILLSGLLLN YSQDEQKLQE SLGQSAAIIA ALVKERNSAL VGQLLVA
Length:447
Mass (Da):49,136
Last modified:December 21, 2004 - v1
Checksum:iAD883B5816D222AB
GO
Isoform 2 (identifier: Q5RJG7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     309-324: CMKITSTVMDHIGGDI → VSIQRMTWIKCHTFLW
     325-447: Missing.

Show »
Length:324
Mass (Da):35,695
Checksum:i4085DE834F5F32C3
GO
Isoform 3 (identifier: Q5RJG7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     277-286: EKISQEICVV → GVFNLVTVSA
     287-447: Missing.

Show »
Length:286
Mass (Da):30,985
Checksum:iFE87DDD1D5CFE53B
GO
Isoform 4 (identifier: Q5RJG7-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-99: Missing.
     277-286: EKISQEICVV → GVFNLVTVSA
     287-447: Missing.

Show »
Length:187
Mass (Da):20,770
Checksum:iCEDC60A2D423E288
GO
Isoform 5 (identifier: Q5RJG7-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     414-419: DEQKLQ → NFYYGG
     420-447: Missing.

Show »
Length:419
Mass (Da):46,292
Checksum:iFF280F53E42D031F
GO

Sequence cautioni

The sequence BAC32538 differs from that shown. Reason: Frameshift at position 48.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti178A → E in BAC31463 (PubMed:16141072).Curated1
Sequence conflicti228S → N in AAI18039 (PubMed:15489334).Curated1
Sequence conflicti342P → L in AAH24061 (PubMed:15489334).Curated1
Sequence conflicti352R → H in AAH24061 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0346671 – 99Missing in isoform 4. 1 PublicationAdd BLAST99
Alternative sequenceiVSP_034668277 – 286EKISQEICVV → GVFNLVTVSA in isoform 3 and isoform 4. 2 Publications10
Alternative sequenceiVSP_034669287 – 447Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST161
Alternative sequenceiVSP_034670309 – 324CMKIT…IGGDI → VSIQRMTWIKCHTFLW in isoform 2. 2 PublicationsAdd BLAST16
Alternative sequenceiVSP_034671325 – 447Missing in isoform 2. 2 PublicationsAdd BLAST123
Alternative sequenceiVSP_034672414 – 419DEQKLQ → NFYYGG in isoform 5. 1 Publication6
Alternative sequenceiVSP_034673420 – 447Missing in isoform 5. 1 PublicationAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK039004 mRNA Translation: BAC30199.1
AK043122 mRNA Translation: BAC31463.1
AK045940 mRNA Translation: BAC32538.1 Frameshift.
BC024061 mRNA Translation: AAH24061.1
BC086665 mRNA Translation: AAH86665.1
BC096030 mRNA Translation: AAH96030.1
BC118038 mRNA Translation: AAI18039.1
CCDSiCCDS49051.1 [Q5RJG7-1]
RefSeqiNP_001276431.1, NM_001289502.1
NP_001276432.1, NM_001289503.1
NP_001276433.1, NM_001289504.1 [Q5RJG7-3]
NP_848744.2, NM_178629.6 [Q5RJG7-1]
XP_006515307.1, XM_006515244.3 [Q5RJG7-5]
UniGeneiMm.261764

Genome annotation databases

EnsembliENSMUST00000062041; ENSMUSP00000061646; ENSMUSG00000043153 [Q5RJG7-1]
ENSMUST00000220519; ENSMUSP00000152423; ENSMUSG00000043153 [Q5RJG7-3]
ENSMUST00000221452; ENSMUSP00000152115; ENSMUSG00000043153 [Q5RJG7-5]
ENSMUST00000221895; ENSMUSP00000152392; ENSMUSG00000043153 [Q5RJG7-2]
ENSMUST00000223068; ENSMUSP00000152318; ENSMUSG00000043153 [Q5RJG7-4]
GeneIDi75847
KEGGimmu:75847
UCSCiuc007njy.3 mouse [Q5RJG7-3]
uc007njz.3 mouse [Q5RJG7-2]
uc007nkb.3 mouse [Q5RJG7-1]
uc007nkd.1 mouse [Q5RJG7-4]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiISPD_MOUSE
AccessioniPrimary (citable) accession number: Q5RJG7
Secondary accession number(s): Q148Q0
, Q501J8, Q8BR14, Q8C934, Q8CAE0, Q8CIF0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: December 21, 2004
Last modified: May 23, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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