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Protein

Polyprenol reductase

Gene

srd5a3

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT) (By similarity).By similarity

Catalytic activityi

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.
A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta4-steroid + NADPH.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNADP

Enzyme and pathway databases

ReactomeiR-DRE-193048. Androgen biosynthesis.
R-DRE-446199. Synthesis of Dolichyl-phosphate.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprenol reductase (EC:1.3.1.94)
Alternative name(s):
3-oxo-5-alpha-steroid 4-dehydrogenase 3 (EC:1.3.1.22)
Steroid 5-alpha-reductase 3
Short name:
S5AR 3
Short name:
SR type 3
Gene namesi
Name:srd5a3
ORF Names:si:ch211-278f21.3
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 20

Organism-specific databases

ZFINiZDB-GENE-030131-7915. srd5a3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3CytoplasmicSequence analysis3
Transmembranei4 – 24HelicalSequence analysisAdd BLAST21
Topological domaini25 – 67LumenalSequence analysisAdd BLAST43
Transmembranei68 – 88HelicalSequence analysisAdd BLAST21
Topological domaini89 – 114CytoplasmicSequence analysisAdd BLAST26
Transmembranei115 – 135HelicalSequence analysisAdd BLAST21
Topological domaini136 – 150LumenalSequence analysisAdd BLAST15
Transmembranei151 – 171HelicalSequence analysisAdd BLAST21
Topological domaini172 – 185CytoplasmicSequence analysisAdd BLAST14
Transmembranei186 – 206HelicalSequence analysisAdd BLAST21
Topological domaini207 – 255LumenalSequence analysisAdd BLAST49
Transmembranei256 – 276HelicalSequence analysisAdd BLAST21
Topological domaini277 – 309CytoplasmicSequence analysisAdd BLAST33

GO - Cellular componenti

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003986511 – 309Polyprenol reductaseAdd BLAST309

Proteomic databases

PaxDbiQ5RIU9.
PRIDEiQ5RIU9.

Expressioni

Gene expression databases

BgeeiENSDARG00000043307.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000063577.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1640. Eukaryota.
ENOG4111JQM. LUCA.
GeneTreeiENSGT00500000044920.
HOGENOMiHOG000018885.
HOVERGENiHBG057797.
InParanoidiQ5RIU9.
KOiK12345.
OMAiRRFYETN.
OrthoDBiEOG091G0F09.
PhylomeDBiQ5RIU9.
TreeFamiTF315011.

Family and domain databases

InterProiView protein in InterPro
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
PfamiView protein in Pfam
PF02544. Steroid_dh. 1 hit.
PROSITEiView protein in PROSITE
PS50244. S5A_REDUCTASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5RIU9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFHILSIVNI IWLLLALCFG AAFCLNKFSV KLPNRVEHVF QDFIRYGKTK
60 70 80 90 100
ENIKRASWQL VFDLSKRYFY HFYVVSVMWN GLLLLFSIRS VVMSEAFPDW
110 120 130 140 150
IIDVLGSLTG RSRGAWNEIH LSTLLLQVLL WVHTLRRLLE CLFVSVFSDG
160 170 180 190 200
VINVVQYAFG LSYYIILGLT VLCTNDSLPQ SESVSFFNQL TWYHVVGTLL
210 220 230 240 250
FFWASFLQHQ SLSLLAKMRT DSSGKVETLA HKMPCGGWFE LVSCPHYLAE
260 270 280 290 300
LLIYAAMCVC CGCASLTWWM VVLYVLCNQA LAAQLCHEYY RSKFKTYPHH

RKAFIPFVL
Length:309
Mass (Da):35,708
Last modified:December 21, 2004 - v1
Checksum:i89513C21F5D7FEB6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti69F → S in AAI33965 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX088688 Genomic DNA. Translation: CAI11971.1.
BC133964 mRNA. Translation: AAI33965.1.
RefSeqiNP_001038404.1. NM_001044939.1.
UniGeneiDr.81044.

Genome annotation databases

EnsembliENSDART00000063578; ENSDARP00000063577; ENSDARG00000043307.
GeneIDi560717.
KEGGidre:560717.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPORED_DANRE
AccessioniPrimary (citable) accession number: Q5RIU9
Secondary accession number(s): A4FVI8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: December 21, 2004
Last modified: June 7, 2017
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families