Reviewed, UniProtKB/Swiss-Prot Q5RIC0 (ELP3_DANRE)

Names and origin

Protein names

Recommended name:
    Elongator complex protein 3
    EC=2.3.1.48

Gene names

Name:	elp3
ORF Names:	si:ch211-63o20.8, zgc:113140

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalytic histone acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling. May also have a methyltransferase activity By similarity.
Catalytic activity	Acetyl-CoA + histone = CoA + acetylhistone.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Subunit structure	Component of the RNA polymerase II elongator complex (Elongator). Elongator associates with the C-terminal domain (CTD) of Pol II largest subunit By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the ELP3 family. Contains 1 N-acetyltransferase domain.
Sequence caution	The sequence CAI12039.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	axonogenesis Inferred from mutant phenotype. Source: ZFIN regulation of transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB transcription elongation factor complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	histone acetyltransferase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW iron-sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorylase kinase regulator activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5RIC0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q5RIC0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q5RIC0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-93: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 548	548	Elongator complex protein 3		PRO_0000283989
Regions
	Domain	397 – 548	152	N-acetyltransferase
Sites
	Metal binding	100	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	110	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	113	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Natural variations
	Alternative sequence	1 – 93	93	Missing in isoform 3.		VSP_026085
	Alternative sequence	1 – 15	15	Missing in isoform 2.		VSP_026084
Experimental info
	Sequence conflict	263	1	H → Y in AAH91787. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 12, 2007. Version 3. Checksum: 8761FBFC0FE684DE	FASTA	548	62,259
	10 20 30 40 50 60 MGKPKKKSDL SRAELMMMTI ADVIKQLVEA HEEGKDINLN KVKTKTSAKY GLSAQPRLVD 70 80 90 100 110 120 IIAAVPPHYR RALVPKLKAK PIRTASGIAV VAVMCKPHRC PHISFTGNIC VYCPGGPDSD 130 140 150 160 170 180 FEYSTQSYTG YEPTSMRAIR ARYDPYLQTR HRVEQLKQLG HSVDKVEFIV MGGTFMALPE 190 200 210 220 230 240 EYRDYFIRNL HDALSGHTSN NVTEAVRYSE RSNTKCVGIT IETRPDYCLK RHLSDMLGYG 250 260 270 280 290 300 CTRLEIGVQS VYEDVARDTN RGHTVRAVCE SFHLAKDAGF KVVAHMMPDL PNVGMERDVE 310 320 330 340 350 360 QFIEFFENPA FRPDGLKLYP TLVIRGTGLY ELWKTGRYKS YSPSALVDLV ARILALVPPW 370 380 390 400 410 420 TRVYRVQRDI PMPLVSSGVE HGNLRELALA RMKDMGTECR DVRTREVGIQ EIHHKVRPYQ 430 440 450 460 470 480 VELIRRDYVA NGGWETFLSY EDPEQDILIG LLRLRRCSPQ SFRPELKGGV SIVRELHVYG 490 500 510 520 530 540 SVVPVSSRDP SKFQHQGFGM MLMEEAERIA RDEHGSSKLA VISGVGTRNY YRKMGYELEG PYMVKNLY
	Isoform 2. Checksum: 70C0C16D4E4E0129	FASTA	533	60,589
	10 20 30 40 50 60 MMMTIADVIK QLVEAHEEGK DINLNKVKTK TSAKYGLSAQ PRLVDIIAAV PPHYRRALVP 70 80 90 100 110 120 KLKAKPIRTA SGIAVVAVMC KPHRCPHISF TGNICVYCPG GPDSDFEYST QSYTGYEPTS 130 140 150 160 170 180 MRAIRARYDP YLQTRHRVEQ LKQLGHSVDK VEFIVMGGTF MALPEEYRDY FIRNLHDALS 190 200 210 220 230 240 GHTSNNVTEA VRYSERSNTK CVGITIETRP DYCLKRHLSD MLGYGCTRLE IGVQSVYEDV 250 260 270 280 290 300 ARDTNRGHTV RAVCESFHLA KDAGFKVVAH MMPDLPNVGM ERDVEQFIEF FENPAFRPDG 310 320 330 340 350 360 LKLYPTLVIR GTGLYELWKT GRYKSYSPSA LVDLVARILA LVPPWTRVYR VQRDIPMPLV 370 380 390 400 410 420 SSGVEHGNLR ELALARMKDM GTECRDVRTR EVGIQEIHHK VRPYQVELIR RDYVANGGWE 430 440 450 460 470 480 TFLSYEDPEQ DILIGLLRLR RCSPQSFRPE LKGGVSIVRE LHVYGSVVPV SSRDPSKFQH 490 500 510 520 530 QGFGMMLMEE AERIARDEHG SSKLAVISGV GTRNYYRKMG YELEGPYMVK NLY
	Isoform 3. Checksum: 1A1227439D70C4E7	FASTA	455	52,132
	10 20 30 40 50 60 MCKPHRCPHI SFTGNICVYC PGGPDSDFEY STQSYTGYEP TSMRAIRARY DPYLQTRHRV 70 80 90 100 110 120 EQLKQLGHSV DKVEFIVMGG TFMALPEEYR DYFIRNLHDA LSGHTSNNVT EAVRYSERSN 130 140 150 160 170 180 TKCVGITIET RPDYCLKRHL SDMLGYGCTR LEIGVQSVYE DVARDTNRGH TVRAVCESFH 190 200 210 220 230 240 LAKDAGFKVV AHMMPDLPNV GMERDVEQFI EFFENPAFRP DGLKLYPTLV IRGTGLYELW 250 260 270 280 290 300 KTGRYKSYSP SALVDLVARI LALVPPWTRV YRVQRDIPMP LVSSGVEHGN LRELALARMK 310 320 330 340 350 360 DMGTECRDVR TREVGIQEIH HKVRPYQVEL IRRDYVANGG WETFLSYEDP EQDILIGLLR 370 380 390 400 410 420 LRRCSPQSFR PELKGGVSIV RELHVYGSVV PVSSRDPSKF QHQGFGMMLM EEAERIARDE 430 440 450 HGSSKLAVIS GVGTRNYYRK MGYELEGPYM VKNLY

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX279523 Genomic DNA. Translation: CAI12039.1. Sequence problems. BC091787 mRNA. Translation: AAH91787.1. BC096810 mRNA. Translation: AAH96810.1.
IPI	IPI00504377. IPI00851479. IPI00851846.
RefSeq	NP_001014344.2.
UniGene	Dr.84313
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5RIC0.
Genome annotation databases
Ensembl	ENSDART00000061557; ENSDARP00000061556; ENSDARG00000042005; Danio rerio. [Genome view]
GeneID	541509.
KEGG	dre:541509.
Organism-specific databases
CTD	541509.
ZFIN	ZDB-GENE-050327-35. elp3.
Phylogenomic databases
HOVERGEN	Q5RIC0.
OMA	WQHRGYG.
Enzyme and pathway databases
BRENDA	2.3.1.48. 96826.
Gene expression databases
Bgee	Q5RIC0.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR006638. Elp3/MiaB/NifB. IPR000182. GCN5-rel_AcTrfase. IPR005910. Hist_AcTrfase_ELP3. IPR007197. Radical_SAM. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00583. Acetyltransf_1. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF005669. Hist_AcTrfase_ELP3. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01211. ELP3. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ELP3_DANRE

Accession

Primary (citable) accession number: Q5RIC0
Secondary accession number(s): Q4V9M7, Q58ER7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	June 12, 2007
Last modified:	November 3, 2009
This is version 37 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project