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Protein

Radical S-adenosyl methionine domain-containing protein 2

Gene

rsad2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Interferon-inducible iron-sulfur (4FE-4S) cluster-binding antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon.By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi81 – 811Iron-sulfur (4Fe-4S-S-AdoMet)Sequence analysis
Metal bindingi85 – 851Iron-sulfur (4Fe-4S-S-AdoMet)Sequence analysis
Metal bindingi88 – 881Iron-sulfur (4Fe-4S-S-AdoMet)Sequence analysis

GO - Molecular functioni

GO - Biological processi

  • defense response to virus Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
  • response to virus Source: ZFIN
Complete GO annotation...

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Radical S-adenosyl methionine domain-containing protein 2
Alternative name(s):
Viperin
Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible
Gene namesi
Name:rsad2Imported
Synonyms:vig1Imported
ORF Names:si:ch211-276e8.2, zgc:112342
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 17

Organism-specific databases

ZFINiZDB-GENE-050913-129. rsad2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359Radical S-adenosyl methionine domain-containing protein 2PRO_0000309588Add
BLAST

Proteomic databases

PaxDbiQ5RH95.

Expressioni

Inductioni

By interferon type I, type II and LPS. Induced by infection with spring viremia of carp virus, presumably through type I interferon pathway.1 Publication

Gene expression databases

BgeeiQ5RH95.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000015681.

Structurei

3D structure databases

ProteinModelPortaliQ5RH95.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. RSAD2 family.Curated

Phylogenomic databases

eggNOGiENOG410IGZE. Eukaryota.
ENOG410XQM5. LUCA.
GeneTreeiENSGT00390000013670.
HOGENOMiHOG000194793.
HOVERGENiHBG053099.
InParanoidiQ5RH95.
KOiK15045.
OMAiINRFNVE.
OrthoDBiEOG7FFMRW.
PhylomeDBiQ5RH95.
TreeFamiTF300085.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
IPR026372. Viperin.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR04278. viperin. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5RH95-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTSNQLGFA RLLMQLCVKN VQSFFLALLR WLSMQVSGAH VQQTPARKIS
60 70 80 90 100
RPESRTSKQK EGSRAPFTTP SSVNYHFTRQ CNYKCGFCFH TAKTSFVLPI
110 120 130 140 150
EEAKRGLRLL KEAGMEKINF SGGEPFVHQK GSFLGELVLY CKQELQLPSV
160 170 180 190 200
SIVSNGSLIR ESWFQKYGDY LDILAISCDS FIEETNQLIG RAQGRKSHLD
210 220 230 240 250
NLHKVRNWCR EYKVAFKINS VINTYNVEED MTEQITALNP VRWKVFQCLL
260 270 280 290 300
IEGENAGENS LREAEKFVIS DQQFQDFLER HQSIQCLVPE SNQKMRDSYL
310 320 330 340 350
ILDEYMRFLD CREGRKDPSK SILDVGVEEA IKFSGFDEKM FLMRGGKYVW

SKADMKLEW
Length:359
Mass (Da):41,475
Last modified:December 21, 2004 - v1
Checksum:iA56821E369A5B5A4
GO

Sequence cautioni

The sequence ABJ97316.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF014961 mRNA. Translation: ABJ97316.1. Different initiation.
BX571665 Genomic DNA. Translation: CAI11967.1.
BC096889 mRNA. Translation: AAH96889.1.
RefSeqiNP_001020727.1. NM_001025556.1.
UniGeneiDr.80053.

Genome annotation databases

EnsembliENSDART00000019617; ENSDARP00000015681; ENSDARG00000004952.
GeneIDi570456.
KEGGidre:570456.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF014961 mRNA. Translation: ABJ97316.1. Different initiation.
BX571665 Genomic DNA. Translation: CAI11967.1.
BC096889 mRNA. Translation: AAH96889.1.
RefSeqiNP_001020727.1. NM_001025556.1.
UniGeneiDr.80053.

3D structure databases

ProteinModelPortaliQ5RH95.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000015681.

Proteomic databases

PaxDbiQ5RH95.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000019617; ENSDARP00000015681; ENSDARG00000004952.
GeneIDi570456.
KEGGidre:570456.

Organism-specific databases

CTDi91543.
ZFINiZDB-GENE-050913-129. rsad2.

Phylogenomic databases

eggNOGiENOG410IGZE. Eukaryota.
ENOG410XQM5. LUCA.
GeneTreeiENSGT00390000013670.
HOGENOMiHOG000194793.
HOVERGENiHBG053099.
InParanoidiQ5RH95.
KOiK15045.
OMAiINRFNVE.
OrthoDBiEOG7FFMRW.
PhylomeDBiQ5RH95.
TreeFamiTF300085.

Miscellaneous databases

PROiQ5RH95.

Gene expression databases

BgeeiQ5RH95.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
IPR026372. Viperin.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR04278. viperin. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of the zebrafish IFN receptor: implications for the origin of the vertebrate IFN system."
    Levraud J.-P., Boudinot P., Colin I., Benmansour A., Peyrieras N., Herbomel P., Lutfalla G.
    J. Immunol. 178:4385-4394(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
  2. "The zebrafish reference genome sequence and its relationship to the human genome."
    Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
    , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
    Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tuebingen.
  3. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiRSAD2_DANRE
AccessioniPrimary (citable) accession number: Q5RH95
Secondary accession number(s): A0FJI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: December 21, 2004
Last modified: June 8, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.