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Protein

Inosine-5'-monophosphate dehydrogenase 1b

Gene

impdh1b

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

K+UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

Pathwayi: XMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes XMP from IMP.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Inosine-5'-monophosphate dehydrogenase (impdh2), Inosine-5'-monophosphate dehydrogenase (impdh1a), Inosine-5'-monophosphate dehydrogenase 2 (impdh2), Inosine-5'-monophosphate dehydrogenase (impdh1b), Inosine-5'-monophosphate dehydrogenase (impdh1b), Inosine-5'-monophosphate dehydrogenase 1a (impdh1a), Inosine-5'-monophosphate dehydrogenase 1b (impdh1b), Inosine-5'-monophosphate dehydrogenase (impdh2), Inosine-5'-monophosphate dehydrogenase (impdh1a)
This subpathway is part of the pathway XMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes XMP from IMP, the pathway XMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi326Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi328Potassium; via carbonyl oxygenUniRule annotation1
Binding sitei329IMPUniRule annotation1
Active sitei331Thioimidate intermediateUniRule annotation1
Metal bindingi331Potassium; via carbonyl oxygenUniRule annotation1
Active sitei429Proton acceptorUniRule annotation1
Binding sitei441IMPUniRule annotation1
Metal bindingi500Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1
Metal bindingi501Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1
Metal bindingi502Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi274 – 276NADUniRule annotation3
Nucleotide bindingi324 – 326NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGMP biosynthesis, Purine biosynthesis
LigandMetal-binding, NAD, Potassium

Enzyme and pathway databases

ReactomeiR-DRE-6798695 Neutrophil degranulation
R-DRE-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00601; UER00295

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenase 1bUniRule annotation (EC:1.1.1.205UniRule annotation)
Short name:
IMP dehydrogenase 1bUniRule annotation
Short name:
IMPD 1bUniRule annotation
Short name:
IMPDH 1bUniRule annotation
Gene namesi
Name:impdh1b
ORF Names:si:dkey-31f5.7
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 4

Organism-specific databases

ZFINiZDB-GENE-030219-206 impdh1b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004156771 – 514Inosine-5'-monophosphate dehydrogenase 1bAdd BLAST514

Proteomic databases

PaxDbiQ5RGV1
PRIDEiQ5RGV1

Expressioni

Gene expression databases

BgeeiENSDARG00000029524
ExpressionAtlasiQ5RGV1 baseline and differential

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi7955.ENSDARP00000066972

Structurei

3D structure databases

ProteinModelPortaliQ5RGV1
SMRiQ5RGV1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini114 – 174CBS 1UniRule annotationAdd BLAST61
Domaini179 – 237CBS 2UniRule annotationAdd BLAST59

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni364 – 366IMP bindingUniRule annotation3
Regioni387 – 388IMP bindingUniRule annotation2
Regioni411 – 415IMP bindingUniRule annotation5

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.UniRule annotation

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

eggNOGiKOG2550 Eukaryota
COG0516 LUCA
COG0517 LUCA
GeneTreeiENSGT00530000062923
HOGENOMiHOG000165752
HOVERGENiHBG052122
InParanoidiQ5RGV1
KOiK00088
OMAiDQQCVVS

Family and domain databases

CDDicd00381 IMPDH, 1 hit
Gene3Di3.20.20.70, 2 hits
HAMAPiMF_01964 IMPDH, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR000644 CBS_dom
IPR005990 IMP_DH
IPR015875 IMP_DH/GMP_Rdtase_CS
IPR001093 IMP_DH_GMPRt
PfamiView protein in Pfam
PF00571 CBS, 2 hits
PF00478 IMPDH, 1 hit
PIRSFiPIRSF000130 IMPDH, 1 hit
SMARTiView protein in SMART
SM00116 CBS, 2 hits
TIGRFAMsiTIGR01302 IMP_dehydrog, 1 hit
PROSITEiView protein in PROSITE
PS51371 CBS, 2 hits
PS00487 IMP_DH_GMP_RED, 1 hit

Sequencei

Sequence statusi: Complete.

Q5RGV1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADYLISGGT GYIPDDGLSA QQLFAVGDGL TYNDFLILPG FIDFTSDEVD
60 70 80 90 100
LTSALTKKIT LKTPLISSPM DTVTESSMAI AMALMGGIGI IHHNCTPEFQ
110 120 130 140 150
ANEVRKVKRF EQGFITDPVV LSPHHTVGDV LEAKVRHGFS GIPITETGKM
160 170 180 190 200
GSKLVGIVTS RDIDFLSEKD NNKYLEEAMT KREDLVVAPA GVTLKEANDI
210 220 230 240 250
LQRSKKGKLP IVNDKDELVA IIARTDLKKN RDYPLASKDS RKQLLCGAAI
260 270 280 290 300
GTREDDKYRL DLLTQSGVDM VVLDSSQGNS VYQINMIHYI KQKYPELQVV
310 320 330 340 350
GGNVVTAAQA KNLIDAGVDA LRVGMGCGSI CITQEVMACG RPQGTSVYKV
360 370 380 390 400
AEYARRFGVP VIADGGIQTV GHVVKALSLG ASTVMMGSLL AATTEAPGEY
410 420 430 440 450
FFSDGVRLKK YRGMGSLDAM EKNTSSQKRY FSEGDKVKVA QGVSGSVQDK
460 470 480 490 500
GSIHKFVPYL IAGIQHGCQD IGAKSLSVLR SMMYSGELKF EKRTMSAQVE
510
GGVHGLHSFE KRLY
Length:514
Mass (Da):55,641
Last modified:December 21, 2004 - v1
Checksum:iB2B97D97618C2466
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX649534 Genomic DNA Translation: CAI21139.1
BC091790 mRNA Translation: AAH91790.1
RefSeqiNP_001014391.1, NM_001014369.2
UniGeneiDr.150513
Dr.43252

Genome annotation databases

EnsembliENSDART00000066975; ENSDARP00000066974; ENSDARG00000029524
GeneIDi338306
KEGGidre:338306

Similar proteinsi

Entry informationi

Entry nameiIMDH3_DANRE
AccessioniPrimary (citable) accession number: Q5RGV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: December 21, 2004
Last modified: May 23, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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