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Protein

Atypical kinase ADCK3, mitochondrial

Gene

adck3

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Atypical kinase involved in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration. Its substrate specificity is unclear: either acts as protein kinase that phosphorylates other proteins in the CoQ complex to stabilize their interactions or acts as a small molecule kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway. Shows an unusual selectivity for binding ADP over ATP.By similarity

Enzyme regulationi

Autoinhibited by the N-terminal domain, containing the KxGQ motif, that completely occludes the typical substrate binding pocket.By similarity

Pathwayi: ubiquinone biosynthesis

This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei327 – 3271ATPBy similarity
Active sitei457 – 4571Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi305 – 3139ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubiquinone biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00232.

Names & Taxonomyi

Protein namesi
Recommended name:
Atypical kinase ADCK3, mitochondrialCurated (EC:2.7.-.-By similarity)
Alternative name(s):
Chaperone activity of bc1 complex-likeBy similarity
Short name:
Chaperone-ABC1-likeBy similarity
aarF domain-containing protein kinase 3
Gene namesi
Name:adck3
Synonyms:cabc1
ORF Names:zgc:92578
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 20

Organism-specific databases

ZFINiZDB-GENE-040718-487. adck3.

Subcellular locationi

  • Mitochondrion By similarity
  • Membrane Sequence analysis; Single-pass membrane protein Sequence analysisBy similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei182 – 19817HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 619Atypical kinase ADCK3, mitochondrialPRO_0000271796
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

PaxDbiQ5RGU1.
PRIDEiQ5RGU1.

Expressioni

Gene expression databases

BgeeiQ5RGU1.

Interactioni

Subunit structurei

Homodimer; homodimerizes via its transmembrane region.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000024635.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini298 – 487190Protein kinaseAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi245 – 2484KxGQ motifBy similarity
Motifi306 – 3094AAAS motifBy similarity

Domaini

Adopts an atypical protein kinase-like fold: while it adopts a core fold similar to that of well-characterized protein kinase-like domains, a number of features are positioned to inhibit the kinase activity: (1) an atypical AAAS motif in an alanine-rich (A-rich) loop that replaces the canonical glycine-rich (G-rich) nucleotide-binding loop and limits ATP binding by establishing an unusual selectivity for ADP and (2) an N-terminal domain, containing the KxGQ motif, that completely occludes the typical substrate binding pocket.By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1234. Eukaryota.
COG0661. LUCA.
GeneTreeiENSGT00550000074739.
HOGENOMiHOG000201140.
HOVERGENiHBG061318.
InParanoidiQ5RGU1.
KOiK08869.
OMAiSEFREEH.
OrthoDBiEOG7X9G6H.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR004147. UbiB_dom.
[Graphical view]
PfamiPF03109. ABC1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5RGU1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGDMLLLMR GLARLSQAVI ETQANSLRSG GVQTMQMTAE QAMGVAMQKI
60 70 80 90 100
QEFTGSQQSV SDFSADMDSK YDFTASEQNF ESAAHGGLDS DSVFRDANTG
110 120 130 140 150
AANTYSQAQG KSKLFDGYKD PTSQFTGHTR SYHQDHSSVG GITAEDIEKA
160 170 180 190 200
REAKQNGSKP HKQMLSERAR ERKVPVTRLG RLANFGGLAV GLGIGALAEV
210 220 230 240 250
AKKSLRSEDK NGNKKAVLDS SPFLSEANAE RIVRTLCKVR GAALKLGQML
260 270 280 290 300
SIQDDAFINP QLAKIFERVR QSADFMPIKQ MTKALSNDLG PNWRDKLEMF
310 320 330 340 350
EERPFAAASI GQVHLARMKD GREVAMKIQY PGVAQSINSD VNNLMTVLSM
360 370 380 390 400
SNALPEGLFP EHLIDVMRRE LALECDYIRE AKCARKFKEL LKDHPFFYVP
410 420 430 440 450
DVISELSSQH VLTTELVPGF PLDQAEALTQ ELKNEICKNI LNLCLRELFE
460 470 480 490 500
FRYMQTDPNW SNFFYDPQTH RVALLDFGAT RGFDESFTDV YIEIIKAAAD
510 520 530 540 550
GNREGVLKQS IDMKFLTGYE SKAMVNAHVD AVMILGEAFA SEEPFDFGAQ
560 570 580 590 600
STTERIHNLI PVMLKQRLIP PPEETYSLHR KMGGSFLICS RLNAKISCKD
610
MFEAAYSNYW SGRKKGPSQ
Length:619
Mass (Da):68,951
Last modified:March 4, 2015 - v2
Checksum:i10CBFECBDC43BFC0
GO
Isoform 2 (identifier: Q5RGU1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     25-263: Missing.

Note: No experimental confirmation available.
Show »
Length:380
Mass (Da):43,231
Checksum:iD2AC1883AA760BEC
GO

Sequence cautioni

The sequence CAI11905.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAI12066.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti404 – 4041S → G in AAH76083 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei25 – 263239Missing in isoform 2. 1 PublicationVSP_022355Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX569783, BX649588 Genomic DNA. Translation: CAI11905.1. Sequence problems.
BX649588, BX569783 Genomic DNA. Translation: CAI12066.1. Sequence problems.
BC076083 mRNA. Translation: AAH76083.1.
RefSeqiNP_001002728.2. NM_001002728.2. [Q5RGU1-1]
UniGeneiDr.124145.
Dr.132698.

Genome annotation databases

EnsembliENSDART00000004721; ENSDARP00000013551; ENSDARG00000020123. [Q5RGU1-2]
ENSDART00000020153; ENSDARP00000024635; ENSDARG00000020123. [Q5RGU1-1]
GeneIDi437001.
KEGGidre:437001.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX569783, BX649588 Genomic DNA. Translation: CAI11905.1. Sequence problems.
BX649588, BX569783 Genomic DNA. Translation: CAI12066.1. Sequence problems.
BC076083 mRNA. Translation: AAH76083.1.
RefSeqiNP_001002728.2. NM_001002728.2. [Q5RGU1-1]
UniGeneiDr.124145.
Dr.132698.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000024635.

Proteomic databases

PaxDbiQ5RGU1.
PRIDEiQ5RGU1.

Protocols and materials databases

DNASUi437001.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000004721; ENSDARP00000013551; ENSDARG00000020123. [Q5RGU1-2]
ENSDART00000020153; ENSDARP00000024635; ENSDARG00000020123. [Q5RGU1-1]
GeneIDi437001.
KEGGidre:437001.

Organism-specific databases

CTDi56997.
ZFINiZDB-GENE-040718-487. adck3.

Phylogenomic databases

eggNOGiKOG1234. Eukaryota.
COG0661. LUCA.
GeneTreeiENSGT00550000074739.
HOGENOMiHOG000201140.
HOVERGENiHBG061318.
InParanoidiQ5RGU1.
KOiK08869.
OMAiSEFREEH.
OrthoDBiEOG7X9G6H.

Enzyme and pathway databases

UniPathwayiUPA00232.

Miscellaneous databases

PROiQ5RGU1.

Gene expression databases

BgeeiQ5RGU1.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR004147. UbiB_dom.
[Graphical view]
PfamiPF03109. ABC1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "The zebrafish reference genome sequence and its relationship to the human genome."
    Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
    , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
    Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tuebingen.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: SJD.

Entry informationi

Entry nameiADCK3_DANRE
AccessioniPrimary (citable) accession number: Q5RGU1
Secondary accession number(s): G1K2I4, Q6DH95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: March 4, 2015
Last modified: June 8, 2016
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.